pycom13g22620

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009414
Physical Location & Seq
Reverse (-)
20272453 .. 20280814
8362 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2523 bp
ATGCTCGAAGTCGAGGGCTCTTGTATTTCCTTCTGGTTTTTGATTTTTCAGCAGCCACCGCTCTTAAACCCCCAAAACCTTCCAGCGGCCACGAAAGCGGTTGCTCCGCCGTTGCTGAGAGAGACAGAGATGATTAGGGGCGGCAAGAGCTACGTGTCAGCACATCCTGCCTTCTCCGCCGATGCCAAGCGCCTCCTCGTCTGCACAGGCACCGCCGTCTCCATCTTTAGCACCTCCACCGGTTTGCAGATATCGTCCTTGGAGGGTCACAATGGCTTGGTCACCTCCGTCATAGTAGTTCCGGGCAACAAGGCTCTGAGCTTCTGCTGGACCGCCTCCGTCGACGGCACCATTCGCTATTGGGACTTTGCTGTTCCTGAGTTGATGAAGACCATTGATATCAAAATGCCCATTTTCTCTATGGTTATTCCGTCCATAATGGGCCAACCCGGGAAAGGAACACGAGAGGATGATTCTAATGCAGCTCATGTTTTTGCTTATTTGTCTGTTGAAAATACCCAAGTGCAACAAAACACACCAAAGCCGTTGCGTGGACGGATTCGGAAGTGTAATTTGACTGAATCTCGTTTGGCTGGCGGTGCTCTCTCTGAGACTCGAAAACCAGAGGCTATAACTATCAGTCCCTCAGGAAAATTCTTTGGCATCCGGAACAAACGGAAGCTTCATATATGGAGAGTCCCTGCTATAGACTCGAAACTCATGATTGCTAAGAAGATTACTCTGCATCATACAAGAAACTTTAACGTTCTTGCATTTCATCCAACTCAGGCAATTGTTGCCGCAGGTGATACAAGTGGGAGAATTCTAATCTGGACAGGATTCGGTAAAGGTACATTTTCCGATGGTGATAAATTGATGAAAGGAAGATCAATGAATAATGAGGAAGAAAGAGATGGAGTTAGGGGAGATGACGATGCTGATGCTTCCTCCACCTGGCATTGGCATGCGGCCGGAATAAGTGTACTTTCTTTCTCTTCTGACGGTGCCTATTTATATTCAGGTGGAATGGAAGGAGTTCTTGTGGTCTGGCAGCTAGACACAGGGAAAAAGAAGTTCTTGCCCAGAATTGGATCTCCTCTTCTATATTTGACTGATTCTCCCGACCCTTCACTTTCTTCTATATCTTGTGCAGATAATCAAATTCATATACTAAAGATGCCCTCCATGGAAATCGTGAAGTCTATTTCAGGGATCAAGCTTCCTTATTCATGTCCAGAGATATATGATGGAATATGCAGCAAGTTTGCCTTTGATCACACTGATGGGTTGGTTGCCTTGCGTACCGTGAATTATCGCATCCAATTCTACAGCTTGTTTGATGACCGTGAAATGTCTGAGGTTCAAATATGTGAAAGAAACCATCAACCAGGGGATGAAGTCAGAGTAACAGTGACTTTGGTGTCTTTATCTAAAGATGGGTCTATGATGTGCACTGTTGAAGTTAAGCTTCCCGAGGAGGGGTTAGGTAGTCTTGTTTGTCTTAAATTTTGGGCATCAGGGCCACAGAACAAAAATTTCAGCCTGTCCACTGTTGTTTATGAACCTCACAGGGATGCTAGAATTTCTTCCCTTGCGTTCCATCCTACTCGTCAAATGGTTGCTAGCTCATCATATGGTGGGGATTTCAAGATTTGGGTTTGCAGTGATGAGATTCAGCAGAAGCATGAGGCGCTGCAACATTCTGGGTGGATGTGTCATGCAATAGGTTCATACAAGAATAAGCCAATGACAGCTGCTGCATTTTCTGGCGATGGTTCTGTTCTGGCGGTTGCAGCTGAAAACGTAATCACTCTATGGGACCCTGACAACAATGCACTTGTAGCTGTCATTGGAGAGATGCAGATGCCAATTGCGACCCTATCTTTTGCTGGGAAATCAGAGTATCTCGTGTCTGTCACTAAAGGTTCAAAACCGCAACTATCGGTGTGGAGCATGTCAGAACTATCTGAATCTTGGTCATATAAGCTACACGTAGAAGCTGTTACCTGTGCGGTAGATTCGTCATCATTTGCAGTTCTATCTTTACTAGGAAAAGATGGGGTAATCTTATTATTCAATGCAACAGATCCTGTTCCATTGGCTATGTGGTCTGTAAGGAAGGCTCAGGGAGGCGGGCTTGCTTTTCTCAACAGCGACCAACTTTCTAACCAACAAAATATTTTAGATAGGAACCGTCCACAAGCTTTGCTTGTCTATATAAATGGTGACCATGAATACGTCATCTTTGACCCATATAACATTGAAGCAAAAGAACTTAGTCTAACCAGGCCGGGGAGTCGTGTTGCCCCTGATGAAACAGGGCAATTTGGATATTCATCTATCTACGGGGAGCTACCGAAGTTTGATATAAAGAGAGACCAGACCGTGGCCCCATCTGCTCCATCACAGAGGCCTTGGGAGACCATATTTAGTGGGTCATCTCATGAACTTCCACCCCTAACCAAACTGTGTTCAGATTTTATGGAATCACTGCTAGAAAGGAGGACTGCAATGGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

841

Amino Acids

92.23

Weight (kDa)

6.31

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 45 - 132 5.8e-06 WDR3 first beta-propeller domain
Beta-prop_WDR75_1st PF23869 58 - 282 2.5e-19 WD repeat-containing protein 75 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 228 - 412 1.2e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st PF23869 316 - 386 1.6e-14 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 439 - 702 2.8e-37 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 517 - 668 2.7e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 520 - 696 6.9e-10 THOC3 beta-propeller domain
Beta-prop_EML PF23409 522 - 671 4.6e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 525 - 655 1.9e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 525 - 654 4.6e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 525 - 695 1.9e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 794
AasI GACNNNNNNGTC 1 cut(s) 1420
Acc36I ACCTGC 1 cut(s) 794
AccB1I GGYRCC 3 cut(s) 209, 347, 1004
AccB7I CCANNNNNTGG 1 cut(s) 2386
AccBSI CCGCTC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 666
AclI AACGTT 1 cut(s) 765
AclWI GGATC 3 cut(s) 1099, 1220, 2081
AcoI YGGCCR 2 cut(s) 87, 969
AcsI RAATTY 6 cut(s) 653, 822, 1161, 1505, 1534, 1581
AfaI GTAC 3 cut(s) 853, 984, 1303
AflIII ACRYGT 2 cut(s) 153, 1990
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 7 cut(s) 512, 1364, 1460, 1648, 1929, 2077, 2264
AjnI CCWGG 3 cut(s) 953, 1387, 2285
Alw21I GWGCWC 2 cut(s) 604, 1454
Alw26I GTCTC 5 cut(s) 116, 223, 605, 2370, 2414
Alw44I GTGCAC 1 cut(s) 1450
AlwI GGATC 3 cut(s) 1099, 1220, 2081
AlwNI CAGNNNCTG 2 cut(s) 1757, 2090
Ama87I CYCGRG 2 cut(s) 449, 1472
Aor13HI TCCGGA 1 cut(s) 666
AoxI GGCC 7 cut(s) 87, 442, 969, 1520, 2288, 2388, 2411
ApaLI GTGCAC 1 cut(s) 1450
ApeKI GCWGC 8 cut(s) 52, 482, 1051, 1257, 1693, 1754, 1757, 1793
ApoI RAATTY 6 cut(s) 653, 822, 1161, 1505, 1534, 1581
AsiGI ACCGGT 1 cut(s) 239
Asp700I GAANNNNTTC 2 cut(s) 1035, 1585
AspLEI GCGC 2 cut(s) 192, 1693
AspS9I GGNCC 5 cut(s) 330, 442, 1520, 1819, 2389
AsuC2I CCSGG 4 cut(s) 303, 450, 451, 2292
AsuHPI GGTGA 4 cut(s) 274, 818, 878, 2237
AsuNHI GCTAGC 1 cut(s) 1622
AvaI CYCGRG 2 cut(s) 449, 1472
AvaII GGWCC 2 cut(s) 330, 1819
AxyI CCTNAGG 1 cut(s) 646
BaeGI GKGCMC 1 cut(s) 1454
BanI GGYRCC 3 cut(s) 209, 347, 1004
BanII GRGCYC 1 cut(s) 20
BauI CACGAG 2 cut(s) 462, 1907
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 2 cut(s) 604, 1454
BbvI GCAGC 8 cut(s) 64, 494, 1063, 1269, 1680, 1741, 1744, 1805
BceAI ACGGC 4 cut(s) 94, 200, 361, 529
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 3 cut(s) 955, 1389, 2287
BclI TGATCA 1 cut(s) 1273
BcnI CCSGG 4 cut(s) 303, 450, 451, 2292
BcoDI GTCTC 5 cut(s) 116, 223, 605, 2370, 2414
BfaI CTAG 5 cut(s) 1055, 1578, 1623, 2048, 2495
BfmI CTRYAG 2 cut(s) 705, 1327
BfoI RGCGCY 2 cut(s) 193, 1694
BfuAI ACCTGC 1 cut(s) 794
Bme1390I CCNGG 7 cut(s) 303, 450, 451, 955, 1389, 2287, 2292
Bme18I GGWCC 2 cut(s) 330, 1819
BmeT110I CYCGRG 2 cut(s) 449, 1472
BmgT120I GGNCC 5 cut(s) 330, 442, 1520, 1819, 2389
BmiI GGNNCC 7 cut(s) 211, 349, 1006, 1820, 1821, 2192, 2391
BmrFI CCNGG 7 cut(s) 303, 450, 451, 955, 1389, 2287, 2292
BmtI GCTAGC 1 cut(s) 1626
BpiI GAAGAC 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 2124
BpuMI CCSGG 4 cut(s) 303, 450, 451, 2292
BsaAI YACGTR 2 cut(s) 154, 1993
BsaBI GATNNNNATC 1 cut(s) 2335
BsaI GGTCTC 2 cut(s) 2370, 2414
BsaJI CCNNGG 8 cut(s) 258, 449, 1185, 1388, 1473, 2291, 2385, 2414
BsaWI WCCGGW 2 cut(s) 239, 666
BsaXI ACNNNNNCTCC 4 cut(s) 1026, 1056, 1102, 1132
Bse118I RCCGGY 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 2517
Bse8I GATNNNNATC 1 cut(s) 2335
BseAI TCCGGA 1 cut(s) 666
BseBI CCWGG 3 cut(s) 955, 1389, 2287
BseDI CCNNGG 8 cut(s) 258, 449, 1185, 1388, 1473, 2291, 2385, 2414
BseGI GGATG 9 cut(s) 163, 475, 663, 778, 1317, 1399, 1579, 1600, 1716
BseJI GATNNNNATC 1 cut(s) 2335
BseMI GCAATG 1 cut(s) 2517
BseMII CTCAG 8 cut(s) 107, 308, 369, 600, 660, 800, 1347, 2138
BseRI GAGGAG 3 cut(s) 185, 1086, 1490
BseSI GKGCMC 1 cut(s) 1454
BseX3I CGGCCG 1 cut(s) 969
BseXI GCAGC 8 cut(s) 64, 494, 1063, 1269, 1680, 1741, 1744, 1805
BseYI CCCAGC 1 cut(s) 1889
BsgI GTGCAG 2 cut(s) 187, 1170
Bsh1285I CGRYCG 1 cut(s) 972
BshFI GGCC 7 cut(s) 89, 444, 971, 1522, 2290, 2390, 2413
BshNI GGYRCC 3 cut(s) 209, 347, 1004
BshTI ACCGGT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 972
BsiHKAI GWGCWC 2 cut(s) 604, 1454
BsiHKCI CYCGRG 2 cut(s) 449, 1472
BsiSI CCGG 6 cut(s) 240, 302, 450, 667, 972, 2291
BslFI GGGAC 4 cut(s) 377, 627, 683, 1832
BsmAI GTCTC 5 cut(s) 116, 223, 605, 2370, 2414
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 4 cut(s) 377, 627, 683, 1832
BsnI GGCC 7 cut(s) 89, 444, 971, 1522, 2290, 2390, 2413
Bso31I GGTCTC 2 cut(s) 2370, 2414
BsoBI CYCGRG 2 cut(s) 449, 1472
Bsp1286I GDGCHC 3 cut(s) 20, 604, 1454
Bsp13I TCCGGA 1 cut(s) 666
Bsp143I GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
Bsp19I CCATGG 1 cut(s) 1185
BspANI GGCC 7 cut(s) 89, 444, 971, 1522, 2290, 2390, 2413
BspCNI CTCAG 8 cut(s) 108, 309, 370, 601, 659, 799, 1348, 2137
BspEI TCCGGA 1 cut(s) 666
BspHI TCATGA 2 cut(s) 720, 2443
BspLI GGNNCC 7 cut(s) 211, 349, 1006, 1820, 1821, 2192, 2391
BspMI ACCTGC 1 cut(s) 794
BspOI GCTAGC 1 cut(s) 1626
BspPI GGATC 3 cut(s) 1099, 1220, 2081
BspT107I GGYRCC 3 cut(s) 209, 347, 1004
BspTNI GGTCTC 2 cut(s) 2370, 2414
BsrBI CCGCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 2517
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 8 cut(s) 258, 449, 1185, 1388, 1473, 2291, 2385, 2414
BssMI GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
BssSI CACGAG 2 cut(s) 462, 1907
BssT1I CCWWGG 3 cut(s) 258, 1185, 2414
Bst2BI CACGAG 2 cut(s) 462, 1907
Bst2UI CCWGG 3 cut(s) 955, 1389, 2287
Bst4CI ACNGT 9 cut(s) 1004, 1306, 1346, 1411, 1456, 1552, 2195, 2386, 2469
Bst6I CTCTTC 2 cut(s) 1000, 1104
BstAPI GCANNNNNTGC 2 cut(s) 167, 797
BstBAI YACGTR 2 cut(s) 154, 1993
BstC8I GCNNGC 5 cut(s) 595, 966, 1624, 2135, 2139
BstDSI CCRYGG 2 cut(s) 1185, 2385
BstEII GGTNACC 2 cut(s) 280, 2225
BstF5I GGATG 9 cut(s) 163, 475, 663, 778, 1317, 1399, 1579, 1600, 1716
BstH2I RGCGCY 2 cut(s) 193, 1694
BstHHI GCGC 2 cut(s) 192, 1693
BstKTI GATC 5 cut(s) 890, 1094, 1215, 1276, 2089
BstMAI GTCTC 5 cut(s) 116, 223, 605, 2370, 2414
BstMBI GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
BstMCI CGRYCG 1 cut(s) 972
BstNI CCWGG 3 cut(s) 955, 1389, 2287
BstNSI RCATGY 2 cut(s) 968, 1957
BstPI GGTNACC 2 cut(s) 280, 2225
BstSCI CCNGG 7 cut(s) 301, 448, 449, 953, 1387, 2285, 2290
BstSFI CTRYAG 2 cut(s) 705, 1327
BstSLI GKGCMC 1 cut(s) 1454
BstV1I GCAGC 8 cut(s) 64, 494, 1063, 1269, 1680, 1741, 1744, 1805
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 2 cut(s) 1091, 2086
BstYI RGATCY 2 cut(s) 1091, 2086
BstZI CGGCCG 1 cut(s) 969
Bsu36I CCTNAGG 1 cut(s) 646
BsuRI GGCC 7 cut(s) 89, 444, 971, 1522, 2290, 2390, 2413
BtgI CCRYGG 2 cut(s) 1185, 2385
BtgZI GCGATG 1 cut(s) 1785
BtsCI GGATG 9 cut(s) 163, 475, 663, 778, 1317, 1399, 1579, 1600, 1716
BtsI GCAGTG 2 cut(s) 1669, 2489
BtsIMutI CAGTG 6 cut(s) 1278, 1416, 1452, 1548, 1669, 2489
BveI ACCTGC 1 cut(s) 794
Cac8I GCNNGC 5 cut(s) 595, 966, 1624, 2135, 2139
CaiI CAGNNNCTG 2 cut(s) 1757, 2090
CciI TCATGA 2 cut(s) 720, 2443
CfoI GCGC 2 cut(s) 192, 1693
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 5 cut(s) 330, 442, 1520, 1819, 2389
Cfr9I CCCGGG 1 cut(s) 449
Csp6I GTAC 3 cut(s) 852, 983, 1302
CspAI ACCGGT 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 2187, 2222
CviQI GTAC 3 cut(s) 852, 983, 1302
DpnI GATC 5 cut(s) 889, 1093, 1214, 1275, 2088
DpnII GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
DrdI GACNNNNNNGTC 1 cut(s) 1420
DseDI GACNNNNNNGTC 1 cut(s) 1420
EaeI YGGCCR 2 cut(s) 87, 969
EagI CGGCCG 1 cut(s) 969
Eam1104I CTCTTC 2 cut(s) 1000, 1104
EarI CTCTTC 2 cut(s) 1000, 1104
EciI GGCGGA 2 cut(s) 96, 166
EclXI CGGCCG 1 cut(s) 969
Eco130I CCWWGG 3 cut(s) 258, 1185, 2414
Eco147I AGGCCT 1 cut(s) 2413
Eco24I GRGCYC 1 cut(s) 20
Eco31I GGTCTC 2 cut(s) 2370, 2414
Eco32I GATATC 2 cut(s) 252, 400
Eco47I GGWCC 2 cut(s) 330, 1819
Eco52I CGGCCG 1 cut(s) 969
Eco81I CCTNAGG 1 cut(s) 646
Eco88I CYCGRG 2 cut(s) 449, 1472
Eco91I GGTNACC 2 cut(s) 280, 2225
EcoO109I RGGNCCY 1 cut(s) 1819
EcoO65I GGTNACC 2 cut(s) 280, 2225
EcoRI GAATTC 1 cut(s) 822
EcoRII CCWGG 3 cut(s) 953, 1387, 2285
EcoRV GATATC 2 cut(s) 252, 400
EcoT14I CCWWGG 3 cut(s) 258, 1185, 2414
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 3 cut(s) 258, 1185, 2414
Esp3I CGTCTC 1 cut(s) 223
FaqI GGGAC 4 cut(s) 377, 627, 683, 1832
FauI CCCGC 1 cut(s) 2126
FauNDI CATATG 1 cut(s) 1633
FbaI TGATCA 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 342
FokI GGATG 9 cut(s) 150, 482, 650, 765, 1304, 1406, 1586, 1587, 1723
FriOI GRGCYC 1 cut(s) 20
FspBI CTAG 5 cut(s) 1055, 1578, 1623, 2048, 2495
GlaI GCGC 2 cut(s) 191, 1692
GsaI CCCAGC 1 cut(s) 1893
HaeII RGCGCY 2 cut(s) 193, 1694
HaeIII GGCC 7 cut(s) 89, 444, 971, 1522, 2290, 2390, 2413
HapII CCGG 6 cut(s) 240, 302, 450, 667, 972, 2291
HhaI GCGC 2 cut(s) 192, 1693
Hin6I GCGC 2 cut(s) 190, 1691
HinP1I GCGC 2 cut(s) 190, 1691
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 4 cut(s) 680, 1217, 1466, 2202
HpaII CCGG 6 cut(s) 240, 302, 450, 667, 972, 2291
HphI GGTGA 4 cut(s) 274, 818, 878, 2237
Hpy166II GTNNAC 6 cut(s) 343, 554, 983, 1452, 1548, 2198
Hpy8I GTNNAC 6 cut(s) 343, 554, 983, 1452, 1548, 2198
Hpy99I CGWCG 2 cut(s) 344, 347
HpyAV CCTTC 6 cut(s) 40, 89, 181, 1025, 1137, 2113
HpyCH4III ACNGT 9 cut(s) 1004, 1306, 1346, 1411, 1456, 1552, 2195, 2386, 2469
HpyCH4IV ACGT 5 cut(s) 153, 765, 1803, 1992, 2238
HpySE526I ACGT 5 cut(s) 153, 765, 1803, 1992, 2238
HspAI GCGC 2 cut(s) 190, 1691
KflI GGGWCCC 1 cut(s) 1819
Kpn2I TCCGGA 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 1273
Kzo9I GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
LmnI GCTCC 4 cut(s) 109, 1950, 2350, 2404
Lsp1109I GCAGC 8 cut(s) 64, 494, 1063, 1269, 1680, 1741, 1744, 1805
MaeI CTAG 5 cut(s) 1055, 1578, 1623, 2048, 2495
MaeII ACGT 5 cut(s) 153, 765, 1803, 1992, 2238
MaeIII GTNAC 7 cut(s) 266, 280, 1405, 1411, 1915, 2002, 2225
MalI GATC 5 cut(s) 889, 1093, 1214, 1275, 2088
MbiI CCGCTC 1 cut(s) 61
MboI GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
MboII GAAGA 8 cut(s) 400, 745, 897, 917, 987, 1091, 1128, 1578
MfeI CAATTG 2 cut(s) 792, 1869
MflI RGATCY 2 cut(s) 1091, 2086
MhlI GDGCHC 3 cut(s) 20, 604, 1454
MlyI GAGTC 4 cut(s) 607, 704, 705, 2305
MmeI TCCRAC 1 cut(s) 806
MroI TCCGGA 1 cut(s) 666
MroXI GAANNNNTTC 2 cut(s) 1035, 1585
MseI TTAA 4 cut(s) 65, 762, 1464, 1503
MslI CAYNNNNRTG 3 cut(s) 963, 1281, 1572
MspA1I CMGCKG 3 cut(s) 86, 1754, 1796
MspI CCGG 6 cut(s) 240, 302, 450, 667, 972, 2291
MspR9I CCNGG 7 cut(s) 303, 450, 451, 955, 1389, 2287, 2292
MunI CAATTG 2 cut(s) 792, 1869
MvaI CCWGG 3 cut(s) 955, 1389, 2287
NciI CCSGG 4 cut(s) 303, 450, 451, 2292
NcoI CCATGG 1 cut(s) 1185
NdeI CATATG 1 cut(s) 1633
NdeII GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
NheI GCTAGC 1 cut(s) 1622
NlaIV GGNNCC 7 cut(s) 211, 349, 1006, 1820, 1821, 2192, 2391
NmuCI GTSAC 5 cut(s) 266, 280, 1411, 1915, 2225
NspI RCATGY 2 cut(s) 968, 1957
PaeI GCATGC 1 cut(s) 968
PagI TCATGA 2 cut(s) 720, 2443
PaqCI CACCTGC 1 cut(s) 794
PceI AGGCCT 1 cut(s) 2413
PdmI GAANNNNTTC 2 cut(s) 1035, 1585
PfeI GAWTC 9 cut(s) 473, 559, 581, 840, 1115, 1672, 1970, 2018, 2486
PflMI CCANNNNNTGG 1 cut(s) 2386
PinAI ACCGGT 1 cut(s) 239
PleI GAGTC 4 cut(s) 607, 704, 704, 2304
PpsI GAGTC 4 cut(s) 607, 704, 704, 2304
Ppu21I YACGTR 2 cut(s) 154, 1993
PpuMI RGGWCCY 1 cut(s) 1819
Psp1406I AACGTT 1 cut(s) 765
Psp5II RGGWCCY 1 cut(s) 1819
Psp6I CCWGG 3 cut(s) 953, 1387, 2285
PspEI GGTNACC 2 cut(s) 280, 2225
PspFI CCCAGC 1 cut(s) 1889
PspGI CCWGG 3 cut(s) 953, 1387, 2285
PspN4I GGNNCC 7 cut(s) 211, 349, 1006, 1820, 1821, 2192, 2391
PspPI GGNCC 5 cut(s) 330, 442, 1520, 1819, 2389
PspPPI RGGWCCY 1 cut(s) 1819
PstNI CAGNNNCTG 2 cut(s) 1757, 2090
PsuI RGATCY 2 cut(s) 1091, 2086
PvuII CAGCTG 2 cut(s) 1754, 1796
RsaI GTAC 3 cut(s) 853, 984, 1303
RsaNI GTAC 3 cut(s) 852, 983, 1302
RseI CAYNNNNRTG 3 cut(s) 963, 1281, 1572
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 4 cut(s) 65, 762, 1464, 1503
Sau3AI GATC 5 cut(s) 887, 1091, 1212, 1273, 2086
Sau96I GGNCC 5 cut(s) 330, 442, 1520, 1819, 2389
SchI GAGTC 4 cut(s) 607, 704, 705, 2305
ScrFI CCNGG 7 cut(s) 303, 450, 451, 955, 1389, 2287, 2292
SduI GDGCHC 3 cut(s) 20, 604, 1454
SfcI CTRYAG 2 cut(s) 705, 1327
SgrDI CGTCGACG 1 cut(s) 341
SinI GGWCC 2 cut(s) 330, 1819
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 3 cut(s) 963, 1281, 1572
SphI GCATGC 1 cut(s) 968
SseBI AGGCCT 1 cut(s) 2413
SspI AATATT 1 cut(s) 2179
SspMI CTAG 5 cut(s) 1055, 1578, 1623, 2048, 2495
StuI AGGCCT 1 cut(s) 2413
StyD4I CCNGG 7 cut(s) 301, 448, 449, 953, 1387, 2285, 2290
StyI CCWWGG 3 cut(s) 258, 1185, 2414
TaaI ACNGT 9 cut(s) 1004, 1306, 1346, 1411, 1456, 1552, 2195, 2386, 2469
TaiI ACGT 5 cut(s) 156, 768, 1806, 1995, 2241
TaqI TCGA 5 cut(s) 6, 12, 342, 616, 713
TatI WGTACW 1 cut(s) 982
TauI GCSGC 4 cut(s) 89, 144, 803, 971
TfiI GAWTC 9 cut(s) 473, 559, 581, 840, 1115, 1672, 1970, 2018, 2486
Tru1I TTAA 4 cut(s) 65, 762, 1464, 1503
Tru9I TTAA 4 cut(s) 65, 762, 1464, 1503
TscAI CASTG 6 cut(s) 1285, 1416, 1459, 1555, 1669, 2496
TseFI GTSAC 5 cut(s) 266, 280, 1411, 1915, 2225
TseI GCWGC 8 cut(s) 52, 482, 1051, 1257, 1693, 1754, 1757, 1793
Tsp45I GTSAC 5 cut(s) 266, 280, 1411, 1915, 2225
TspGWI ACGGA 5 cut(s) 277, 328, 420, 571, 691
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 6 cut(s) 1285, 1416, 1459, 1555, 1669, 2496
Van91I CCANNNNNTGG 1 cut(s) 2386
VneI GTGCAC 1 cut(s) 1450
VpaK11BI GGWCC 2 cut(s) 330, 1819
XapI RAATTY 6 cut(s) 653, 822, 1161, 1505, 1534, 1581
XceI RCATGY 2 cut(s) 968, 1957
XcmI CCANNNNNNNNNTGG 2 cut(s) 418, 2103
XmaI CCCGGG 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 1035, 1585
XspI CTAG 5 cut(s) 1055, 1578, 1623, 2048, 2495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.