pycom14g00650

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009055
Physical Location & Seq
Reverse (-)
434526 .. 435284
759 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGCTGATTTTCATACTTGAGCTGCCGCTTTATCTGCCTAGGAGATTGACTATTCCTGTTGTTTGTGAAGAAAGATGGTGTAAGCCGTATGTAGTTGCTTCAGTGACACTAGCACCAGTGCTTTTGTCAACACTTTGGAACCATCAATTTGGAAATTTTAGCTTCAAGACAAAGCTTGCCATCTATATTATCGGTTTGATTTTTGGAATCACTTTTGGGATTGTTGCTTTTTTGACAACTGAGAAGTCAAGCCCACCAAAAAAGTGCTTATTTCCTTGGCTTGCCGCAGGGTTTGTAATGAGTGTTACTTGGAGTTACCTTACAGCTCAAGAATTGGTAGGGTTGTTAGTTTCAATAGGGTACATATCAGGTGTGAGTCCTTCAATATTAGGGCTGACAGTCCTTGCTTGGGGCAACTCACTTGGGGACTTGATTACAAATTTGACAATGGCTTTGAATGGTGGAGCAGAAGGAGCTCAGATTGCATTTTCAGCATGTTATGCTGGCCCTATCTTCAACACTCTGTTTGGATTAGGGTTATCGCTTGTTGGCTCAGCTTGTTCTAAATTTCCGTCACCTGTTTTGATCCAGAGTGATGCATACCTCTTGGAGACCGTTGGTTTCTTGGTTGGCGGCTTGCTTTGGGCACTCGTGGTTCTACCGAGGAGAGGTATGAGGCTTGATGGGGTGTTGGGAGGTGGGCTTTTGGTTGCGTACATCGGCTCCATGTCTTTGAGGTTGATTCAAACACTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

253

Amino Acids

27.11

Weight (kDa)

8.84

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 94 - 245 1.4e-23 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 26, 285, 633
AclWI GGATC 1 cut(s) 580
AcsI RAATTY 3 cut(s) 154, 439, 566
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 2 cut(s) 362, 716
AfiI CCNNNNNNNGG 2 cut(s) 409, 668
AgsI TTSAA 6 cut(s) 166, 354, 384, 457, 517, 746
AluBI AGCT 6 cut(s) 22, 162, 175, 326, 476, 557
AluI AGCT 6 cut(s) 22, 162, 175, 326, 476, 557
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 1 cut(s) 605
AlwI GGATC 1 cut(s) 580
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 1 cut(s) 22
ApoI RAATTY 3 cut(s) 154, 439, 566
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AspA2I CCTAGG 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 567
AvrII CCTAGG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 9
BccI CCATC 4 cut(s) 69, 150, 188, 677
BceAI ACGGC 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 605
BfaI CTAG 2 cut(s) 39, 110
BisI GCNGC 4 cut(s) 23, 26, 285, 634
BlnI CCTAGG 1 cut(s) 38
BlpI GCTNAGC 1 cut(s) 553
BlsI GCNGC 4 cut(s) 24, 27, 286, 635
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 2 cut(s) 140, 724
BmsI GCATC 1 cut(s) 586
Bpu1102I GCTNAGC 1 cut(s) 553
BpuEI CTTGAG 2 cut(s) 38, 312
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 38, 275, 662
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 668
Bse1I ACTGG 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 38, 275, 662
BseLI CCNNNNNNNGG 2 cut(s) 409, 668
BseMII CTCAG 3 cut(s) 231, 491, 567
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 679
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 1 cut(s) 9
BshFI GGCC 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 478
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 409, 668
BsmAI GTCTC 1 cut(s) 605
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 1 cut(s) 507
Bso31I GGTCTC 1 cut(s) 605
Bsp1286I GDGCHC 2 cut(s) 478, 649
Bsp143I GATC 1 cut(s) 585
Bsp1720I GCTNAGC 1 cut(s) 553
BspACI CCGC 3 cut(s) 26, 285, 633
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 3 cut(s) 232, 490, 566
BspLI GGNNCC 2 cut(s) 140, 724
BspPI GGATC 1 cut(s) 580
BspTNI GGTCTC 1 cut(s) 605
BsrI ACTGG 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 38, 275, 662
BssMI GATC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 2 cut(s) 38, 275
Bst2BI CACGAG 1 cut(s) 650
Bst4CI ACNGT 2 cut(s) 400, 616
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 4 cut(s) 177, 282, 505, 638
BstDEI CTNAG 3 cut(s) 240, 477, 553
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 1 cut(s) 605
BstMBI GATC 1 cut(s) 585
BstMWI GCNNNNNNNGC 5 cut(s) 34, 473, 482, 491, 500
BstNSI RCATGY 1 cut(s) 498
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 149
BsuRI GGCC 1 cut(s) 507
BtsIMutI CAGTG 2 cut(s) 108, 123
Cac8I GCNNGC 4 cut(s) 177, 282, 505, 638
Cfr13I GGNCC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 361, 715
CviAII CATG 2 cut(s) 495, 727
CviQI GTAC 2 cut(s) 361, 715
DdeI CTNAG 3 cut(s) 240, 477, 553
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 476
Eco130I CCWWGG 2 cut(s) 38, 275
Eco24I GRGCYC 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 605
Eco53kI GAGCTC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 476
EcoT14I CCWWGG 2 cut(s) 38, 275
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 38, 275
FaeI CATG 2 cut(s) 498, 730
FaiI YATR 9 cut(s) 14, 90, 186, 365, 496, 501, 601, 674, 728
FaqI GGGAC 1 cut(s) 440
FatI CATG 2 cut(s) 494, 726
Fnu4HI GCNGC 4 cut(s) 23, 26, 285, 634
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 4 cut(s) 23, 26, 285, 634
FspBI CTAG 2 cut(s) 39, 110
GluI GCNGC 4 cut(s) 23, 26, 285, 634
HaeIII GGCC 1 cut(s) 507
Hin1II CATG 2 cut(s) 498, 730
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 3 cut(s) 207, 376, 742
HphI GGTGA 1 cut(s) 567
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 1 cut(s) 480
Hpy188III TCNNGA 3 cut(s) 166, 329, 589
Hpy8I GTNNAC 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 390, 464
HpyCH4III ACNGT 2 cut(s) 400, 616
HpyCH4V TGCA 2 cut(s) 485, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 473, 482, 491, 500
HpyF3I CTNAG 3 cut(s) 240, 477, 553
Hsp92II CATG 2 cut(s) 498, 730
Kzo9I GATC 1 cut(s) 585
LmnI GCTCC 3 cut(s) 464, 473, 728
LpnPI CCDG 7 cut(s) 69, 129, 273, 354, 489, 591, 602
Lsp1109I GCAGC 1 cut(s) 9
LweI GCATC 1 cut(s) 586
MaeI CTAG 2 cut(s) 39, 110
MaeIII GTNAC 4 cut(s) 103, 304, 314, 573
MalI GATC 1 cut(s) 587
MboI GATC 1 cut(s) 585
MboII GAAGA 2 cut(s) 80, 505
MhlI GDGCHC 2 cut(s) 478, 649
MluCI AATT 5 cut(s) 146, 154, 332, 439, 566
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 6 cut(s) 614, 657, 662, 669, 689, 729
Mph1103I ATGCAT 1 cut(s) 601
MwoI GCNNNNNNNGC 5 cut(s) 34, 473, 482, 491, 500
NdeII GATC 1 cut(s) 585
NlaIII CATG 2 cut(s) 498, 730
NlaIV GGNNCC 2 cut(s) 140, 724
NmuCI GTSAC 2 cut(s) 103, 573
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 1 cut(s) 498
PfeI GAWTC 2 cut(s) 207, 742
PkrI GCNGC 4 cut(s) 24, 27, 286, 635
PleI GAGTC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 140, 724
PspPI GGNCC 1 cut(s) 506
RsaI GTAC 2 cut(s) 362, 716
RsaNI GTAC 2 cut(s) 361, 715
SacI GAGCTC 1 cut(s) 478
SatI GCNGC 4 cut(s) 23, 26, 285, 634
Sau3AI GATC 1 cut(s) 585
Sau96I GGNCC 1 cut(s) 506
SchI GAGTC 1 cut(s) 385
SduI GDGCHC 2 cut(s) 478, 649
SfaNI GCATC 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 17, 327
SmoI CTYRAG 2 cut(s) 17, 327
Sse9I AATT 5 cut(s) 146, 154, 332, 439, 566
SsiI CCGC 3 cut(s) 26, 285, 633
SspI AATATT 1 cut(s) 387
SspMI CTAG 2 cut(s) 39, 110
SstI GAGCTC 1 cut(s) 478
StyI CCWWGG 2 cut(s) 38, 275
TaaI ACNGT 2 cut(s) 400, 616
TasI AATT 5 cut(s) 146, 154, 332, 439, 566
TauI GCSGC 3 cut(s) 28, 287, 636
TfiI GAWTC 2 cut(s) 207, 742
TscAI CASTG 2 cut(s) 108, 123
TseFI GTSAC 2 cut(s) 103, 573
TseI GCWGC 1 cut(s) 22
Tsp45I GTSAC 2 cut(s) 103, 573
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 2 cut(s) 108, 123
XapI RAATTY 3 cut(s) 154, 439, 566
XceI RCATGY 1 cut(s) 498
XmaJI CCTAGG 1 cut(s) 38
XspI CTAG 2 cut(s) 39, 110
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.