pycom14g01260
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011326
Physical Location & Seq
Reverse (-)
887654 .. 890569
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGTGCCACCGTCGCAATCGTGCTAGTACAGTTGTGGTCGGAGCGAGCTCCAGGAAAAGAGGATTGAGGAGGCTGAACGCGGTGGCGGAGACGGCGGCGATGGAGGCAGTGGAGCTGAAGAAGGGGATTGCTGAGCTGTACGACGAGTCGTCTGGCTTGTGGGAGAACATCTGGGGCGACCACATGCACCACGGATACTACGACCCAGACGCCGACGTTTCGGTCTCCATTCCCGACCATCGCGCCGCCCAGATTCGCATGATCGACGAGGTCCTGCGATTCGCCGGCATTTCTGAGGAGCCCCCAACTAGAATTCCTAAGAATTTGGTGGACGTGGGATGCGGGATAGGAGGGAGCTCCAGATATCTGGCTAGTAAATACGGGGCCAATGCCAAAGGCATCACCCTCAGCCCTGTGCAGGCTGAAAGGGCCAATGCTCTTGCTGCTGCTCAAGGGTTAGCCAACCAGGCTTCGTTTCAAGTTGCAGATGCTCTGGACCAACCATTTCCTGACGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGAGAGCACATGCCTGACAAAGCCAAGTTTGTGAACGAGTTGGTTCGAGTTGCTGCACCAGGAGCAACAATAATTATAGTCACATGGTGCCACAGGGATCTTGGTGCTTCTGAAAAAACCCTGAAGCCAGATGAGAAGAGTCTCCTGGACAGGATATGCAATTCATTCTATCTTCCAGCCTGGTGTTCCACTGCTGATTATCTCAAATTACTCGAGTCCCACTCGCTCGAGGACATCAAGGCGGAAGATTGGTCCCCTTATGTTGCCCCGTTTTGGCCTGCAGTTATCCGCTCGGCATTGACATGGAAGGGTTTTACATCGCTGTTACTCACTGGGATAAAAACAATAAAAGGAGCATTGGTAATGCCATTGATGATCCAAGGATTCAAGAAGGACCTAATCAAGTATTCGGTGATTACGTGCCGAAAGCCGGAATAA

Protein Analysis

328

Amino Acids

36.02

Weight (kDa)

6.47

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 86 - 224 4e-13 Methyltransferase domain
CMAS PF02353 107 - 208 3.9e-11 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 108 - 219 2.1e-14 Methyltransferase domain
Methyltransf_11 PF08241 110 - 208 1.6e-20 Methyltransferase domain
Methyltransf_25 PF13649 110 - 203 5.4e-18 Methyltransferase domain
Methyltransf_12 PF08242 110 - 205 7.3e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccB1I GGYRCC 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 837
AccII CGCG 2 cut(s) 80, 243
AciI CCGC 7 cut(s) 80, 86, 95, 246, 342, 788, 835
AclWI GGATC 2 cut(s) 651, 916
AcsI RAATTY 3 cut(s) 312, 322, 518
AcuI CTGAAG 2 cut(s) 137, 689
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 633
AfaI GTAC 2 cut(s) 28, 140
AfiI CCNNNNNNNGG 3 cut(s) 418, 819, 976
AgsI TTSAA 2 cut(s) 479, 934
AhdI GACNNNNNGTC 1 cut(s) 148
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 5 cut(s) 50, 465, 604, 690, 725
AluBI AGCT 4 cut(s) 48, 115, 136, 357
AluI AGCT 4 cut(s) 48, 115, 136, 357
Alw21I GWGCWC 3 cut(s) 50, 359, 555
Alw26I GTCTC 3 cut(s) 83, 229, 692
AlwI GGATC 2 cut(s) 651, 916
Ama87I CYCGRG 2 cut(s) 758, 773
AoxI GGCC 3 cut(s) 384, 429, 821
ApeKI GCWGC 3 cut(s) 443, 446, 599
ApoI RAATTY 3 cut(s) 312, 322, 518
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
AsuHPI GGTGA 2 cut(s) 394, 970
AvaI CYCGRG 2 cut(s) 758, 773
AvaII GGWCC 5 cut(s) 271, 496, 534, 798, 940
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 3 cut(s) 50, 303, 359
Bbv12I GWGCWC 3 cut(s) 50, 359, 555
BbvCI CCTCAGC 1 cut(s) 407
BbvI GCAGC 3 cut(s) 430, 433, 586
BccI CCATC 2 cut(s) 94, 246
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 5 cut(s) 52, 467, 606, 692, 727
BciVI GTATCC 1 cut(s) 188
BcoDI GTCTC 3 cut(s) 83, 229, 692
BfaI CTAG 3 cut(s) 24, 309, 372
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 5 cut(s) 96, 246, 444, 447, 600
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 5 cut(s) 97, 247, 445, 448, 601
Bme1390I CCNGG 5 cut(s) 52, 467, 606, 692, 727
Bme18I GGWCC 5 cut(s) 271, 496, 534, 798, 940
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 2 cut(s) 758, 773
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
BmiI GGNNCC 4 cut(s) 300, 385, 635, 800
BmrFI CCNGG 5 cut(s) 52, 467, 606, 692, 727
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 3 cut(s) 329, 408, 478
BmuI ACTGGG 1 cut(s) 888
BplI GAGNNNNNCTC 2 cut(s) 752, 784
BpmI CTGGAG 2 cut(s) 34, 343
Bpu10I CCTNAGC 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 966
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 3 cut(s) 190, 537, 925
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 819, 976
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 883
BseBI CCWGG 5 cut(s) 52, 467, 606, 692, 727
BseDI CCNNGG 3 cut(s) 190, 537, 925
BseGI GGATG 1 cut(s) 344
BseLI CCNNNNNNNGG 3 cut(s) 418, 819, 976
BseMII CTCAG 3 cut(s) 123, 285, 421
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 2 cut(s) 82, 311
BseXI GCAGC 3 cut(s) 430, 433, 586
BsgI GTGCAG 2 cut(s) 437, 585
Bsh1236I CGCG 2 cut(s) 80, 243
BshFI GGCC 3 cut(s) 386, 431, 823
BshNI GGYRCC 1 cut(s) 633
BsiHKAI GWGCWC 3 cut(s) 50, 359, 555
BsiHKCI CYCGRG 2 cut(s) 758, 773
BsiSI CCGG 2 cut(s) 285, 977
BslFI GGGAC 2 cut(s) 748, 784
BslI CCNNNNNNNGG 3 cut(s) 418, 819, 976
BsmAI GTCTC 3 cut(s) 83, 229, 692
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 748, 784
BsnI GGCC 3 cut(s) 386, 431, 823
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 758, 773
Bsp1286I GDGCHC 4 cut(s) 50, 303, 359, 555
Bsp143I GATC 4 cut(s) 261, 523, 643, 921
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 7 cut(s) 80, 86, 95, 246, 342, 788, 835
BspANI GGCC 3 cut(s) 386, 431, 823
BspCNI CTCAG 3 cut(s) 124, 286, 420
BspFNI CGCG 2 cut(s) 80, 243
BspLI GGNNCC 4 cut(s) 300, 385, 635, 800
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 2 cut(s) 651, 916
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 883
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 190, 537, 925
BssMI GATC 4 cut(s) 261, 523, 643, 921
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 537, 925
Bst2UI CCWGG 5 cut(s) 52, 467, 606, 692, 727
Bst4CI ACNGT 2 cut(s) 11, 31
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 966
BstC8I GCNNGC 4 cut(s) 46, 286, 420, 825
BstDEI CTNAG 4 cut(s) 132, 294, 318, 407
BstDSI CCRYGG 2 cut(s) 190, 537
BstF5I GGATG 1 cut(s) 344
BstFNI CGCG 2 cut(s) 80, 243
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 4 cut(s) 264, 526, 646, 924
BstMAI GTCTC 3 cut(s) 83, 229, 692
BstMBI GATC 4 cut(s) 261, 523, 643, 921
BstMWI GCNNNNNNNGC 7 cut(s) 12, 92, 104, 428, 443, 467, 608
BstNI CCWGG 5 cut(s) 52, 467, 606, 692, 727
BstNSI RCATGY 2 cut(s) 187, 559
BstSCI CCNGG 5 cut(s) 50, 465, 604, 690, 725
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 2 cut(s) 80, 243
BstV1I GCAGC 3 cut(s) 430, 433, 586
BstX2I RGATCY 1 cut(s) 643
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 643
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 3 cut(s) 386, 431, 823
BtgI CCRYGG 2 cut(s) 190, 537
BtgZI GCGATG 3 cut(s) 113, 224, 849
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 344
BtsI GCAGTG 2 cut(s) 114, 735
BtsIMutI CAGTG 3 cut(s) 114, 735, 876
Cac8I GCNNGC 4 cut(s) 46, 286, 420, 825
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 27, 139
CviAII CATG 6 cut(s) 184, 259, 538, 556, 630, 849
CviQI GTAC 2 cut(s) 27, 139
DdeI CTNAG 4 cut(s) 132, 294, 318, 407
DpnI GATC 4 cut(s) 263, 525, 645, 923
DpnII GATC 4 cut(s) 261, 523, 643, 921
DraIII CACNNNGTG 1 cut(s) 633
DrdI GACNNNNNNGTC 1 cut(s) 221
DriI GACNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 677
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 677
EciI GGCGGA 2 cut(s) 101, 803
Ecl136II GAGCTC 2 cut(s) 48, 357
Eco130I CCWWGG 2 cut(s) 537, 925
Eco24I GRGCYC 3 cut(s) 50, 303, 359
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 5 cut(s) 271, 496, 534, 798, 940
Eco53kI GAGCTC 2 cut(s) 48, 357
Eco57I CTGAAG 2 cut(s) 137, 689
Eco88I CYCGRG 2 cut(s) 758, 773
EcoICRI GAGCTC 2 cut(s) 48, 357
EcoO109I RGGNCCY 2 cut(s) 271, 940
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 5 cut(s) 50, 465, 604, 690, 725
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 2 cut(s) 537, 925
EcoT38I GRGCYC 3 cut(s) 50, 303, 359
ErhI CCWWGG 2 cut(s) 537, 925
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 6 cut(s) 187, 262, 541, 559, 633, 852
FaqI GGGAC 2 cut(s) 748, 784
FatI CATG 6 cut(s) 183, 258, 537, 555, 629, 848
FauI CCCGC 1 cut(s) 335
Fnu4HI GCNGC 5 cut(s) 96, 246, 444, 447, 600
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 3 cut(s) 50, 303, 359
Fsp4HI GCNGC 5 cut(s) 96, 246, 444, 447, 600
FspBI CTAG 3 cut(s) 24, 309, 372
GlaI GCGC 1 cut(s) 244
GluI GCNGC 5 cut(s) 96, 246, 444, 447, 600
GsuI CTGGAG 2 cut(s) 34, 343
HaeIII GGCC 3 cut(s) 386, 431, 823
HapII CCGG 2 cut(s) 285, 977
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 6 cut(s) 187, 262, 541, 559, 633, 852
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 6 cut(s) 146, 253, 279, 685, 761, 930
HpaII CCGG 2 cut(s) 285, 977
HphI GGTGA 2 cut(s) 394, 970
Hpy166II GTNNAC 2 cut(s) 331, 580
Hpy188I TCNGA 3 cut(s) 41, 295, 658
Hpy188III TCNNGA 5 cut(s) 233, 360, 494, 509, 934
Hpy8I GTNNAC 2 cut(s) 331, 580
Hpy99I CGWCG 4 cut(s) 15, 146, 218, 269
HpyAV CCTTC 3 cut(s) 115, 847, 931
HpyCH4III ACNGT 2 cut(s) 11, 31
HpyCH4IV ACGT 3 cut(s) 216, 333, 965
HpyCH4V TGCA 6 cut(s) 187, 418, 485, 602, 705, 827
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 92, 104, 428, 443, 467, 608
HpyF3I CTNAG 4 cut(s) 132, 294, 318, 407
HpySE526I ACGT 3 cut(s) 216, 333, 965
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 6 cut(s) 187, 262, 541, 559, 633, 852
HspAI GCGC 1 cut(s) 243
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Kzo9I GATC 4 cut(s) 261, 523, 643, 921
LmnI GCTCC 8 cut(s) 41, 53, 112, 298, 354, 362, 608, 899
Lsp1109I GCAGC 3 cut(s) 430, 433, 586
LweI GCATC 3 cut(s) 329, 408, 478
MaeI CTAG 3 cut(s) 24, 309, 372
MaeII ACGT 3 cut(s) 216, 333, 965
MaeIII GTNAC 2 cut(s) 625, 870
MalI GATC 4 cut(s) 263, 525, 645, 923
MbiI CCGCTC 1 cut(s) 837
MboI GATC 4 cut(s) 261, 523, 643, 921
MboII GAAGA 4 cut(s) 130, 694, 710, 803
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 4 cut(s) 50, 303, 359, 555
MluCI AATT 6 cut(s) 312, 322, 518, 618, 706, 752
MlyI GAGTC 3 cut(s) 155, 694, 770
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 9 cut(s) 53, 60, 63, 97, 262, 289, 344, 416, 769
MroNI GCCGGC 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 847
MspI CCGG 2 cut(s) 285, 977
MspR9I CCNGG 5 cut(s) 52, 467, 606, 692, 727
MvaI CCWGG 5 cut(s) 52, 467, 606, 692, 727
MvnI CGCG 2 cut(s) 80, 243
MwoI GCNNNNNNNGC 7 cut(s) 12, 92, 104, 428, 443, 467, 608
NaeI GCCGGC 1 cut(s) 286
NcoI CCATGG 1 cut(s) 537
NdeII GATC 4 cut(s) 261, 523, 643, 921
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 6 cut(s) 187, 262, 541, 559, 633, 852
NlaIV GGNNCC 4 cut(s) 300, 385, 635, 800
NmeAIII GCCGAG 1 cut(s) 818
NmuCI GTSAC 1 cut(s) 625
NspI RCATGY 2 cut(s) 187, 559
PaeR7I CTCGAG 2 cut(s) 758, 773
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 962
PdiI GCCGGC 1 cut(s) 286
PfeI GAWTC 3 cut(s) 253, 279, 930
PflFI GACNNNGTC 1 cut(s) 269
PfoI TCCNGGA 2 cut(s) 50, 690
PkrI GCNGC 5 cut(s) 97, 247, 445, 448, 601
PleI GAGTC 3 cut(s) 154, 693, 769
PpsI GAGTC 3 cut(s) 154, 693, 769
Ppu21I YACGTR 1 cut(s) 966
PpuMI RGGWCCY 2 cut(s) 271, 940
Psp124BI GAGCTC 2 cut(s) 50, 359
Psp5II RGGWCCY 2 cut(s) 271, 940
Psp6I CCWGG 5 cut(s) 50, 465, 604, 690, 725
PspGI CCWGG 5 cut(s) 50, 465, 604, 690, 725
PspN4I GGNNCC 4 cut(s) 300, 385, 635, 800
PspPI GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
PspPPI RGGWCCY 2 cut(s) 271, 940
PspXI VCTCGAGB 2 cut(s) 758, 773
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 1 cut(s) 643
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 2 cut(s) 28, 140
RsaNI GTAC 2 cut(s) 27, 139
RseI CAYNNNNRTG 1 cut(s) 847
SacI GAGCTC 2 cut(s) 50, 359
SatI GCNGC 5 cut(s) 96, 246, 444, 447, 600
Sau3AI GATC 4 cut(s) 261, 523, 643, 921
Sau96I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
SchI GAGTC 3 cut(s) 155, 694, 770
ScrFI CCNGG 5 cut(s) 52, 467, 606, 692, 727
SduI GDGCHC 4 cut(s) 50, 303, 359, 555
SetI ASST 9 cut(s) 50, 117, 138, 219, 273, 336, 359, 945, 968
SfaNI GCATC 3 cut(s) 329, 408, 478
SfcI CTRYAG 1 cut(s) 825
Sfr274I CTCGAG 2 cut(s) 758, 773
SinI GGWCC 5 cut(s) 271, 496, 534, 798, 940
SlaI CTCGAG 2 cut(s) 758, 773
SmiMI CAYNNNNRTG 1 cut(s) 847
SmlI CTYRAG 3 cut(s) 450, 758, 773
SmoI CTYRAG 3 cut(s) 450, 758, 773
Sse9I AATT 6 cut(s) 312, 322, 518, 618, 706, 752
SsiI CCGC 7 cut(s) 80, 86, 95, 246, 342, 788, 835
SspMI CTAG 3 cut(s) 24, 309, 372
SstI GAGCTC 2 cut(s) 50, 359
StyD4I CCNGG 5 cut(s) 50, 465, 604, 690, 725
StyI CCWWGG 2 cut(s) 537, 925
TaaI ACNGT 2 cut(s) 11, 31
TaiI ACGT 3 cut(s) 219, 336, 968
TaqI TCGA 4 cut(s) 264, 592, 759, 774
TaqII GACCGA 1 cut(s) 211
TasI AATT 6 cut(s) 312, 322, 518, 618, 706, 752
TatI WGTACW 1 cut(s) 26
TauI GCSGC 2 cut(s) 98, 248
TfiI GAWTC 3 cut(s) 253, 279, 930
TscAI CASTG 3 cut(s) 114, 742, 883
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 3 cut(s) 443, 446, 599
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 699
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 3 cut(s) 114, 742, 883
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 5 cut(s) 271, 496, 534, 798, 940
XapI RAATTY 3 cut(s) 312, 322, 518
XceI RCATGY 2 cut(s) 187, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XhoI CTCGAG 2 cut(s) 758, 773
XspI CTAG 3 cut(s) 24, 309, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.