pycom14g02250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014262
Physical Location & Seq
Reverse (-)
1740534 .. 1741430
897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 897 bp
ATGCCACAAGTCGATTTGGAAACACTAGTTTCGGCGTGTGCGGGGGGCTCAATGGATCGGAAAATCGCCTGCGAGACGCAGGCTGACGCCACCACTGCCGGACACGACCGGCCGGAAGAAGATGCCGATGAACCGCAAGTTGCGCCGGATTTCCCGCCGGAATCCTTCTGGCTCTCGAAAGACGCAGAGTACGAGTGGTTCGACCGCAACGCCTTCTACGAGCGCAAAGACTCCACCAAAGGAAACTCCAACGCCAATAACTTGAATTCGAATCAATATTCGCACTCGAATTCGAGCTCGCAGCGGTTTTCGAAGAACTTGAAGTCGAAGGCGGCGATCATCGGGCTTCCGAAGCCGCAGAAGCACAACTACGCCGACCCGAAGAACCGGCGGCACTCCAAGGCCGGAAACACGAGACTGTTCCCGAAACGGACCGGATCCGTCGGGAAATCGGACGCTCCGATGATCGAGCCGTCGTCTCCTAACGTCTCGTGTATGGGGAGGGTGAGATCGAAGAGGGATAGAAAGCGTCGGTTCAGGAATCGGCACGGATCGTCGGCCGAGACGTCCATGGAGAAGTCCGTTAAACCGGTGGCGGAGAGACGAAAACTCGGTTTCTTTGCGAGTTTTCGGGCGATTTTTAGGCACGGAGGACACCGGGAGAAATCGGCGAAATCGCCGTTCGTGGAAGACTCGCCGCCGAGAAACAGCAGCATGATGTCGACGTCGAGAGCGCGCGACAGGGCGGCGGCGTACGATTTCGACGCGCTGTCTATCGACTCGACGCCGAGACACAGCGTGGAGAGCGAACCGCCTGGTTTGGGCGGCATGAAGCGGTTCGTTTCGGGTCGGAGGTCCGGGGCGTGGGCGGGCGAAGTCGGTGTCGACGTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

33.03

Weight (kDa)

9.81

Isoelectric Point (pI)

59.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014262)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36220 AT3G52710
fragaria_vesca FvH4_6g18850
malus_domestica MD12G1024700.v1.1 MD14G1023600.v1.1
prunus_persica Prupe.7G109200_v2.0.a1
pyrus_communis pycom12g02440 pycom14g02250
rosa_laevigata RLG00000023978
rosa_multiflora Rmu_sc0005561.1_g000001
rosa_roxburghii Rroxscaffold_6G00407720
rosa_rugosa Rorug03G0136100
rosa_samantha Rh3AG186500 Rh3BG215100 Rh3CG211800 Rh3DG211000
rosa_wichuraiana Rw3G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 569, 728
AccI GTMKAC 2 cut(s) 722, 885
AccII CGCG 3 cut(s) 736, 738, 767
AclWI GGATC 4 cut(s) 63, 432, 445, 559
AcoI YGGCCR 2 cut(s) 110, 558
AcsI RAATTY 2 cut(s) 265, 289
AcyI GRCGYC 4 cut(s) 87, 566, 725, 785
AdeI CACNNNGTG 1 cut(s) 799
AfaI GTAC 2 cut(s) 191, 755
AfiI CCNNNNNNNGG 2 cut(s) 821, 864
AgeI ACCGGT 1 cut(s) 589
AgsI TTSAA 2 cut(s) 265, 322
AhdI GACNNNNNGTC 1 cut(s) 769
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 1 cut(s) 814
AluBI AGCT 1 cut(s) 297
AluI AGCT 1 cut(s) 297
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 7 cut(s) 68, 409, 483, 493, 557, 595, 784
AlwI GGATC 4 cut(s) 63, 432, 445, 559
AoxI GGCC 3 cut(s) 110, 402, 558
ApeKI GCWGC 2 cut(s) 301, 711
ApoI RAATTY 2 cut(s) 265, 289
ArsI GACNNNNNNTTYG 1 cut(s) 867
AsiGI ACCGGT 1 cut(s) 589
Asp700I GAANNNNTTC 1 cut(s) 836
AspLEI GCGC 5 cut(s) 145, 225, 736, 738, 769
AspS9I GGNCC 2 cut(s) 432, 855
AsuC2I CCSGG 2 cut(s) 659, 859
AsuHPI GGTGA 1 cut(s) 517
AsuII TTCGAA 2 cut(s) 269, 311
AvaII GGWCC 2 cut(s) 432, 855
BamHI GGATCC 1 cut(s) 437
BanII GRGCYC 2 cut(s) 50, 299
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 2 cut(s) 412, 490
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 313, 723
BceAI ACGGC 2 cut(s) 457, 664
BciT130I CCWGG 1 cut(s) 816
BcnI CCSGG 2 cut(s) 659, 859
BcoDI GTCTC 7 cut(s) 68, 409, 483, 493, 557, 595, 784
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 1 cut(s) 26
BisI GCNGC 9 cut(s) 302, 333, 356, 392, 698, 712, 747, 750, 826
BlsI GCNGC 9 cut(s) 303, 334, 357, 393, 699, 713, 748, 751, 827
Bme1390I CCNGG 3 cut(s) 659, 816, 859
Bme18I GGWCC 2 cut(s) 432, 855
BmeRI GACNNNNNGTC 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 432, 855
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 3 cut(s) 659, 816, 859
BmsI GCATC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 696
Bpu14I TTCGAA 2 cut(s) 269, 311
BpuMI CCSGG 2 cut(s) 659, 859
BsaHI GRCGYC 4 cut(s) 87, 566, 725, 785
BsaJI CCNNGG 3 cut(s) 399, 570, 858
BsaWI WCCGGW 2 cut(s) 434, 589
Bsc4I CCNNNNNNNGG 2 cut(s) 821, 864
Bse118I RCCGGY 3 cut(s) 108, 387, 589
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 3 cut(s) 399, 570, 858
BseLI CCNNNNNNNGG 2 cut(s) 821, 864
BsePI GCGCGC 1 cut(s) 734
BseX3I CGGCCG 2 cut(s) 110, 558
BseXI GCAGC 2 cut(s) 313, 723
Bsh1236I CGCG 3 cut(s) 736, 738, 767
Bsh1285I CGRYCG 4 cut(s) 109, 113, 205, 561
BshFI GGCC 3 cut(s) 112, 404, 560
BshTI ACCGGT 1 cut(s) 589
BsiEI CGRYCG 4 cut(s) 109, 113, 205, 561
BsiHKAI GWGCWC 1 cut(s) 299
BsiWI CGTACG 1 cut(s) 753
BslI CCNNNNNNNGG 2 cut(s) 821, 864
BsmAI GTCTC 7 cut(s) 68, 409, 483, 493, 557, 595, 784
BsmBI CGTCTC 5 cut(s) 68, 483, 493, 557, 595
BsnI GGCC 3 cut(s) 112, 404, 560
Bsp119I TTCGAA 2 cut(s) 269, 311
Bsp1286I GDGCHC 2 cut(s) 50, 299
Bsp143I GATC 6 cut(s) 55, 336, 437, 465, 509, 551
Bsp19I CCATGG 1 cut(s) 570
BspANI GGCC 3 cut(s) 112, 404, 560
BspFNI CGCG 3 cut(s) 736, 738, 767
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 4 cut(s) 63, 432, 445, 559
BspT104I TTCGAA 2 cut(s) 269, 311
BsrFI RCCGGY 3 cut(s) 108, 387, 589
BssAI RCCGGY 3 cut(s) 108, 387, 589
BssECI CCNNGG 3 cut(s) 399, 570, 858
BssHII GCGCGC 1 cut(s) 734
BssMI GATC 6 cut(s) 55, 336, 437, 465, 509, 551
BssNI GRCGYC 4 cut(s) 87, 566, 725, 785
BssSI CACGAG 2 cut(s) 412, 490
BssT1I CCWWGG 2 cut(s) 399, 570
Bst2BI CACGAG 2 cut(s) 412, 490
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 1 cut(s) 420
Bst6I CTCTTC 1 cut(s) 509
BstACI GRCGYC 4 cut(s) 87, 566, 725, 785
BstBI TTCGAA 2 cut(s) 269, 311
BstC8I GCNNGC 5 cut(s) 70, 81, 299, 736, 871
BstDSI CCRYGG 1 cut(s) 570
BstFNI CGCG 3 cut(s) 736, 738, 767
BstHHI GCGC 5 cut(s) 145, 225, 736, 738, 769
BstKTI GATC 6 cut(s) 58, 339, 440, 468, 512, 554
BstMAI GTCTC 7 cut(s) 68, 409, 483, 493, 557, 595, 784
BstMBI GATC 6 cut(s) 55, 336, 437, 465, 509, 551
BstMCI CGRYCG 4 cut(s) 109, 113, 205, 561
BstMWI GCNNNNNNNGC 5 cut(s) 95, 142, 352, 361, 804
BstNI CCWGG 1 cut(s) 816
BstSCI CCNGG 3 cut(s) 657, 814, 857
BstUI CGCG 3 cut(s) 736, 738, 767
BstV1I GCAGC 2 cut(s) 313, 723
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
BstZI CGGCCG 2 cut(s) 110, 558
BsuRI GGCC 3 cut(s) 112, 404, 560
BtgI CCRYGG 1 cut(s) 570
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 5 cut(s) 70, 81, 299, 736, 871
CfoI GCGC 5 cut(s) 145, 225, 736, 738, 769
Cfr10I RCCGGY 3 cut(s) 108, 387, 589
Cfr13I GGNCC 2 cut(s) 432, 855
CpoI CGGWCCG 1 cut(s) 432
CseI GACGC 7 cut(s) 85, 95, 191, 464, 518, 773, 793
Csp6I GTAC 2 cut(s) 190, 754
CspAI ACCGGT 1 cut(s) 589
CspI CGGWCCG 1 cut(s) 432
CviAII CATG 3 cut(s) 571, 715, 829
CviQI GTAC 2 cut(s) 190, 754
DpnI GATC 6 cut(s) 57, 338, 439, 467, 511, 553
DpnII GATC 6 cut(s) 55, 336, 437, 465, 509, 551
DraIII CACNNNGTG 1 cut(s) 799
DriI GACNNNNNGTC 1 cut(s) 769
EaeI YGGCCR 2 cut(s) 110, 558
EagI CGGCCG 2 cut(s) 110, 558
Eam1104I CTCTTC 1 cut(s) 509
Eam1105I GACNNNNNGTC 1 cut(s) 769
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 1 cut(s) 611
Ecl136II GAGCTC 1 cut(s) 297
EclXI CGGCCG 2 cut(s) 110, 558
Eco130I CCWWGG 2 cut(s) 399, 570
Eco24I GRGCYC 2 cut(s) 50, 299
Eco47I GGWCC 2 cut(s) 432, 855
Eco52I CGGCCG 2 cut(s) 110, 558
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRI GAATTC 2 cut(s) 265, 289
EcoRII CCWGG 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 399, 570
EcoT38I GRGCYC 2 cut(s) 50, 299
ErhI CCWWGG 2 cut(s) 399, 570
Esp3I CGTCTC 5 cut(s) 68, 483, 493, 557, 595
FaeI CATG 3 cut(s) 574, 718, 832
FaiI YATR 4 cut(s) 497, 572, 716, 830
FatI CATG 3 cut(s) 570, 714, 828
FauI CCCGC 3 cut(s) 34, 162, 862
FblI GTMKAC 2 cut(s) 722, 885
Fnu4HI GCNGC 9 cut(s) 302, 333, 356, 392, 698, 712, 747, 750, 826
FriOI GRGCYC 2 cut(s) 50, 299
Fsp4HI GCNGC 9 cut(s) 302, 333, 356, 392, 698, 712, 747, 750, 826
FspBI CTAG 1 cut(s) 26
GlaI GCGC 5 cut(s) 144, 224, 735, 737, 768
GluI GCNGC 9 cut(s) 302, 333, 356, 392, 698, 712, 747, 750, 826
HaeIII GGCC 3 cut(s) 112, 404, 560
HgaI GACGC 7 cut(s) 85, 95, 191, 464, 518, 773, 793
HhaI GCGC 5 cut(s) 145, 225, 736, 738, 769
Hin1I GRCGYC 4 cut(s) 87, 566, 725, 785
Hin1II CATG 3 cut(s) 574, 718, 832
Hin6I GCGC 5 cut(s) 143, 223, 734, 736, 767
HinP1I GCGC 5 cut(s) 143, 223, 734, 736, 767
HincII GTYRAC 2 cut(s) 723, 886
HindII GTYRAC 2 cut(s) 723, 886
HinfI GANTC 6 cut(s) 161, 230, 271, 541, 692, 779
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 2 cut(s) 723, 886
Hpy188I TCNGA 5 cut(s) 60, 351, 454, 462, 852
Hpy188III TCNNGA 5 cut(s) 175, 424, 445, 538, 729
Hpy8I GTNNAC 2 cut(s) 723, 886
Hpy99I CGWCG 9 cut(s) 446, 478, 534, 559, 727, 730, 767, 787, 890
HpyAV CCTTC 3 cut(s) 175, 223, 322
HpyCH4III ACNGT 1 cut(s) 420
HpyCH4IV ACGT 4 cut(s) 486, 566, 725, 888
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 142, 352, 361, 804
HpySE526I ACGT 4 cut(s) 486, 566, 725, 888
Hsp92I GRCGYC 4 cut(s) 87, 566, 725, 785
Hsp92II CATG 3 cut(s) 574, 718, 832
HspAI GCGC 5 cut(s) 143, 223, 734, 736, 767
Kzo9I GATC 6 cut(s) 55, 336, 437, 465, 509, 551
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 2 cut(s) 313, 723
LweI GCATC 1 cut(s) 112
MaeI CTAG 1 cut(s) 26
MaeII ACGT 4 cut(s) 486, 566, 725, 888
MalI GATC 6 cut(s) 57, 338, 439, 467, 511, 553
MboI GATC 6 cut(s) 55, 336, 437, 465, 509, 551
MboII GAAGA 6 cut(s) 128, 131, 325, 394, 526, 701
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 2 cut(s) 50, 299
MluCI AATT 2 cut(s) 265, 289
MlyI GAGTC 3 cut(s) 224, 686, 773
MmeI TCCRAC 2 cut(s) 273, 830
MnlI CCTC 4 cut(s) 495, 510, 644, 846
MroXI GAANNNNTTC 1 cut(s) 836
MseI TTAA 1 cut(s) 585
MspA1I CMGCKG 1 cut(s) 304
MspR9I CCNGG 3 cut(s) 659, 816, 859
MvaI CCWGG 1 cut(s) 816
MvnI CGCG 3 cut(s) 736, 738, 767
MwoI GCNNNNNNNGC 5 cut(s) 95, 142, 352, 361, 804
NciI CCSGG 2 cut(s) 659, 859
NcoI CCATGG 1 cut(s) 570
NdeII GATC 6 cut(s) 55, 336, 437, 465, 509, 551
NlaIII CATG 3 cut(s) 574, 718, 832
NlaIV GGNNCC 1 cut(s) 439
NmeAIII GCCGAG 3 cut(s) 586, 726, 813
NspV TTCGAA 2 cut(s) 269, 311
PauI GCGCGC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 7 cut(s) 189, 198, 216, 332, 458, 563, 885
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 3 cut(s) 161, 271, 541
Pfl23II CGTACG 1 cut(s) 753
PinAI ACCGGT 1 cut(s) 589
PkrI GCNGC 9 cut(s) 303, 334, 357, 393, 699, 713, 748, 751, 827
PleI GAGTC 3 cut(s) 224, 686, 773
PpsI GAGTC 3 cut(s) 224, 686, 773
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspLI CGTACG 1 cut(s) 753
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 2 cut(s) 432, 855
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PsuI RGATCY 1 cut(s) 437
PteI GCGCGC 1 cut(s) 734
RsaI GTAC 2 cut(s) 191, 755
RsaNI GTAC 2 cut(s) 190, 754
Rsr2I CGGWCCG 1 cut(s) 432
RsrII CGGWCCG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 299
SalI GTCGAC 2 cut(s) 721, 884
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 9 cut(s) 302, 333, 356, 392, 698, 712, 747, 750, 826
Sau3AI GATC 6 cut(s) 55, 336, 437, 465, 509, 551
Sau96I GGNCC 2 cut(s) 432, 855
SchI GAGTC 3 cut(s) 224, 686, 773
ScrFI CCNGG 3 cut(s) 659, 816, 859
SduI GDGCHC 2 cut(s) 50, 299
SetI ASST 6 cut(s) 299, 489, 569, 728, 857, 891
SfaNI GCATC 1 cut(s) 112
SfuI TTCGAA 2 cut(s) 269, 311
SinI GGWCC 2 cut(s) 432, 855
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 2 cut(s) 265, 289
SspI AATATT 1 cut(s) 278
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 657, 814, 857
StyI CCWWGG 2 cut(s) 399, 570
TaaI ACNGT 1 cut(s) 420
TaiI ACGT 4 cut(s) 489, 569, 728, 891
TasI AATT 2 cut(s) 265, 289
TauI GCSGC 7 cut(s) 335, 358, 394, 700, 749, 752, 828
TfiI GAWTC 3 cut(s) 161, 271, 541
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 100
TseI GCWGC 2 cut(s) 301, 711
TspDTI ATGAA 2 cut(s) 144, 845
TspGWI ACGGA 5 cut(s) 430, 445, 564, 571, 663
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 432, 855
XapI RAATTY 2 cut(s) 265, 289
XmiI GTMKAC 2 cut(s) 722, 885
XmnI GAANNNNTTC 1 cut(s) 836
XspI CTAG 1 cut(s) 26
ZraI GACGTC 2 cut(s) 567, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.