pycom14g02290

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013403
Physical Location & Seq
Forward (+)
1763672 .. 1765978
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGAACCGCACACGCACGCAAACAGTAACACGGCATCCGGTTCCAGTGATTACACTGCAGTTTCATTCACCTACAAACCCCTCCAAGTTACCTCACGAGCTACAACCATTAGAAAACTGCCACATAATATTTTTTTTTTTAAATTTCATCTTTTCACCTCTCACACGTGTTGTTTTCTATGCAAGTAGTGACGGGAGTGATAATATAACACCTCCGTCTCTCCTCCCTCAAACTCTCTCTCCTCTCTCTCTCTCCTTCTCTCTCTCTCTAAACAGCAGTGCAATTGGGTTCGCCATGGCCGGAAGAAAGAGGAAGCTGAAATCGTTAGCTGAAACAATTACAAATAGTAATAGAAAAGCAAGCAAGACAAAAAAGGAGAAACTGGACCTGCAAACTTGGTCTGACCTTCCTGCTGAACTTTTGGAACTCATTGTGTCTACTTTAACTCTGCGGGATAACATTCGTGTATCTGTTGTATGCAAGAGATGGCACTCAGCTGCCATATCTGTGCGGGTGGTCAACCAATCACCACTGCTTATGTACTTTCCCAGATTTGGTGACCTATATGAATTCTATGATCGATCTCAGCGCAAAATCTACTCCTTGGAGTTACCTGAGCTGACTGGGTCTAGGATTTGCTACACTAAAGACGGTTGGCTACTGTTATACAGACCCAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

228

Amino Acids

25.97

Weight (kDa)

9.85

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 133 - 171 2.5e-08 F-box domain
F-box-like PF12937 133 - 167 3.2e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57790
fragaria_vesca FvH4_6g18890
malus_domestica MD12G1025100.v1.1 MD14G1023800.v1.1
prunus_persica Prupe.7G109000_v2.0.a1
pyrus_communis pycom12g02460 pycom14g02290
rosa_chinensis RchiOBHm_Chr3g0473881
rosa_laevigata RLG00000023976
rosa_multiflora Rmu_sc0002414.1_g000044
rosa_roxburghii Rroxscaffold_6G00407700
rosa_rugosa Rorug03G0136200 Rorug03G0136300
rosa_samantha Rh3AG186800 Rh3BG215400 Rh3CG212100 Rh3DG211300
rosa_wichuraiana Rw3G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 437
AciI CCGC 3 cut(s) 7, 451, 511
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 2 cut(s) 142, 569
AcvI CACGTG 1 cut(s) 167
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 1 cut(s) 554
AflIII ACRYGT 2 cut(s) 164, 166
AluBI AGCT 5 cut(s) 100, 316, 329, 497, 619
AluI AGCT 5 cut(s) 100, 316, 329, 497, 619
Alw26I GTCTC 1 cut(s) 222
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 2 cut(s) 142, 569
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 385
AsuHPI GGTGA 4 cut(s) 60, 147, 519, 569
AvaII GGWCC 1 cut(s) 385
BauI CACGAG 1 cut(s) 95
BbrPI CACGTG 1 cut(s) 167
BbvI GCAGC 1 cut(s) 484
BccI CCATC 1 cut(s) 480
BceAI ACGGC 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 1 cut(s) 630
BfmI CTRYAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 396
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 42
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 633
Bpu10I CCTNAGC 1 cut(s) 615
Bsa29I ATCGAT 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 294, 603
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse1I ACTGG 3 cut(s) 44, 387, 628
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 294, 603
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMII CTCAG 3 cut(s) 507, 599, 606
BseNI ACTGG 3 cut(s) 44, 387, 628
BseRI GAGGAG 2 cut(s) 212, 231
BseXI GCAGC 1 cut(s) 484
BshFI GGCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 580
BsiSI CCGG 2 cut(s) 38, 300
BslI CCNNNNNNNGG 1 cut(s) 554
BsmAI GTCTC 1 cut(s) 222
BsmBI CGTCTC 1 cut(s) 222
BsnI GGCC 1 cut(s) 299
Bsp143I GATC 2 cut(s) 577, 581
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 3 cut(s) 7, 451, 511
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 3 cut(s) 506, 598, 607
BspDI ATCGAT 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 396
BsrI ACTGG 3 cut(s) 44, 387, 628
BssECI CCNNGG 2 cut(s) 294, 603
BssMI GATC 2 cut(s) 577, 581
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 2 cut(s) 294, 603
Bst2BI CACGAG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 25, 653, 663
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 17, 361
BstDEI CTNAG 3 cut(s) 493, 585, 615
BstDSI CCRYGG 1 cut(s) 294
BstEII GGTNACC 1 cut(s) 557
BstF5I GGATG 1 cut(s) 34
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 2 cut(s) 580, 584
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 2 cut(s) 577, 581
BstPI GGTNACC 1 cut(s) 557
BstSFI CTRYAG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 580
BsuRI GGCC 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 580
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 3 cut(s) 53, 283, 530
BtsIMutI CAGTG 4 cut(s) 51, 53, 283, 530
BveI ACCTGC 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 17, 361
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 1 cut(s) 385
ClaI ATCGAT 1 cut(s) 580
Csp6I GTAC 1 cut(s) 541
CviAII CATG 1 cut(s) 295
CviJI RGCY 7 cut(s) 100, 299, 316, 329, 497, 619, 658
CviKI_1 RGCY 7 cut(s) 100, 299, 316, 329, 497, 619, 658
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 3 cut(s) 493, 585, 615
DpnI GATC 2 cut(s) 579, 583
DpnII GATC 2 cut(s) 577, 581
DraI TTTAAA 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 294, 603
Eco47I GGWCC 1 cut(s) 385
Eco72I CACGTG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 557
EcoO65I GGTNACC 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 569
EcoT14I CCWWGG 2 cut(s) 294, 603
ErhI CCWWGG 2 cut(s) 294, 603
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 1 cut(s) 298
FatI CATG 1 cut(s) 294
FauI CCCGC 2 cut(s) 444, 504
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 1 cut(s) 630
GlaI GCGC 1 cut(s) 590
GluI GCNGC 1 cut(s) 498
HaeIII GGCC 1 cut(s) 299
HapII CCGG 2 cut(s) 38, 300
HhaI GCGC 1 cut(s) 591
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HpaII CCGG 2 cut(s) 38, 300
HphI GGTGA 4 cut(s) 60, 147, 519, 569
Hpy166II GTNNAC 2 cut(s) 438, 520
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 438, 520
HpyAV CCTTC 2 cut(s) 265, 416
HpyCH4III ACNGT 3 cut(s) 25, 653, 663
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 58, 182, 281, 391, 480
HpyF3I CTNAG 3 cut(s) 493, 585, 615
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 1 cut(s) 589
Kzo9I GATC 2 cut(s) 577, 581
LpnPI CCDG 9 cut(s) 51, 57, 313, 368, 401, 423, 562, 609, 627
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 630
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 5 cut(s) 25, 87, 188, 557, 609
MalI GATC 2 cut(s) 579, 583
MboI GATC 2 cut(s) 577, 581
MboII GAAGA 1 cut(s) 315
MfeI CAATTG 1 cut(s) 282
MluCI AATT 4 cut(s) 142, 282, 336, 569
MnlI CCTC 8 cut(s) 91, 102, 168, 222, 233, 237, 252, 303
MseI TTAA 2 cut(s) 140, 443
MslI CAYNNNNRTG 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 2 cut(s) 38, 300
MunI CAATTG 1 cut(s) 282
NcoI CCATGG 1 cut(s) 294
NdeII GATC 2 cut(s) 577, 581
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 188, 557
PflFI GACNNNGTC 1 cut(s) 625
PkrI GCNGC 1 cut(s) 499
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 167
PspCI CACGTG 1 cut(s) 167
PspEI GGTNACC 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 1 cut(s) 385
PstI CTGCAG 1 cut(s) 60
PsyI GACNNNGTC 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 2 cut(s) 140, 443
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 2 cut(s) 577, 581
Sau96I GGNCC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 56
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 506
Sse9I AATT 4 cut(s) 142, 282, 336, 569
SsiI CCGC 3 cut(s) 7, 451, 511
SspI AATATT 1 cut(s) 129
SspMI CTAG 1 cut(s) 630
StyI CCWWGG 2 cut(s) 294, 603
TaaI ACNGT 3 cut(s) 25, 653, 663
TaiI ACGT 1 cut(s) 169
TaqI TCGA 1 cut(s) 580
TasI AATT 4 cut(s) 142, 282, 336, 569
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 2 cut(s) 140, 443
Tru9I TTAA 2 cut(s) 140, 443
TscAI CASTG 4 cut(s) 51, 60, 283, 537
TseFI GTSAC 2 cut(s) 188, 557
TseI GCWGC 1 cut(s) 497
Tsp45I GTSAC 2 cut(s) 188, 557
TspDTI ATGAA 4 cut(s) 17, 53, 136, 582
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 4 cut(s) 51, 60, 283, 537
Tth111I GACNNNGTC 1 cut(s) 625
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 2 cut(s) 142, 569
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.