pycom14g03510

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005317
Physical Location & Seq
Reverse (-)
2932580 .. 2936189
3610 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGCTATGCAAACTGTGTATTTCAAAGAACATGAAGGAATTGCCCCTAATCCCATAGGGCAGTTTTCATCAGCATTGTCAGGGCCTTGGTGGAATGCCTTTGGATCTCAACCGGTGTATGGGGAATCCTGTGGCCAGTCGAAACCTTTTAACAGGGAACAGCCCAATACCGGGGACCATTTCACCGCCACTAAACTTGCCGGGCAGGGTACTGAACAAGAACTGGAAAAAGGGCACACAAATCTGTTCACTATCTTTCCTGGGGATGGTCAAAAGTCCCAGGCAGCAATTGGTCCGCCAGGAAAATACCGTGGTCATTTTGAGCTTGGATTCAGCCAGCCTATGATCTGTGCAAAATATCCATATATGGATCAATGCTATGGACTCTTCTCAGCTTATGGACCTCAAATCTCGGGACGCATTATGCTGCCGCTGAACTTGACTACGGATGAAGGACCAATCTATGTAAATGCCAAGCAGTACCATGGAATCATCCGTCGCCGACAATCCCGTGCCAAGGCTGTCATTGAGAACAGGGCAGCTAGACTCCGTAAGCCGTACATGCATGAATCGCGCCATCGCCATGCAATGCGCCGACCAAGAGGCTGTGGTGGTCGTTTTCTGAACACCAAGACTATGAACAATGGGAACAAGGGAACTGAAGGGAAGCAAGGTGGTGATGGGCAGCTGTTTAGGCTTTCTGGTTCTCAGAGTCCTGAAGTCCTGCAATCCGACAGTGGAACTTTAAATTCGTCAAGGGAAACAAATGGCAGCAGTTCAAACATATCCGGGTCAGAGGTGTCAAGTATGTACTCTAGGGGAGATCTTGATCGCTTTTCAATCAATCATCTCGGTCCCTCTTTGCACTCTCTGTCAAACATGATGGACAGTGCACGTGGTATGGTCATTCCGACCAAATGGGTTGCAGCAGGAGATAACCGCTGCAACCTCAAAGTTTGA

Protein Analysis

320

Amino Acids

35.07

Weight (kDa)

9.57

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 153 - 207 9e-25 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 574
AciI CCGC 4 cut(s) 186, 296, 431, 940
AclWI GGATC 2 cut(s) 112, 378
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 2 cut(s) 681, 738
AcvI CACGTG 1 cut(s) 896
AfaI GTAC 4 cut(s) 211, 482, 560, 812
AfiI CCNNNNNNNGG 5 cut(s) 119, 170, 171, 266, 517
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 3 cut(s) 25, 780, 840
AjnI CCWGG 3 cut(s) 259, 279, 298
AluBI AGCT 4 cut(s) 325, 395, 542, 688
AluI AGCT 4 cut(s) 325, 395, 542, 688
Alw21I GWGCWC 1 cut(s) 895
Alw44I GTGCAC 1 cut(s) 891
AlwI GGATC 2 cut(s) 112, 378
Ama87I CYCGRG 1 cut(s) 412
AoxI GGCC 2 cut(s) 83, 133
ApaLI GTGCAC 1 cut(s) 891
ApeKI GCWGC 7 cut(s) 284, 427, 539, 685, 771, 926, 942
ApoI RAATTY 1 cut(s) 748
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 2 cut(s) 576, 594
AspS9I GGNCC 6 cut(s) 83, 175, 293, 401, 455, 854
AsuC2I CCSGG 3 cut(s) 172, 202, 790
AsuHPI GGTGA 2 cut(s) 175, 689
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 5 cut(s) 175, 293, 401, 455, 854
BaeGI GKGCMC 2 cut(s) 237, 895
BalI TGGCCA 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 7 cut(s) 296, 414, 551, 697, 783, 929, 938
BccI CCATC 4 cut(s) 260, 585, 674, 877
BceAI ACGGC 1 cut(s) 541
BciT130I CCWGG 3 cut(s) 261, 281, 300
BcnI CCSGG 3 cut(s) 172, 202, 790
BfaI CTAG 2 cut(s) 543, 816
BglII AGATCT 1 cut(s) 823
BisI GCNGC 8 cut(s) 285, 428, 431, 540, 686, 772, 927, 943
BlsI GCNGC 8 cut(s) 286, 429, 432, 541, 687, 773, 928, 944
Bme1390I CCNGG 6 cut(s) 172, 202, 261, 281, 300, 790
Bme18I GGWCC 5 cut(s) 175, 293, 401, 455, 854
BmeT110I CYCGRG 1 cut(s) 412
BmgT120I GGNCC 6 cut(s) 83, 175, 293, 401, 455, 854
BmiI GGNNCC 2 cut(s) 176, 856
BmrFI CCNGG 6 cut(s) 172, 202, 261, 281, 300, 790
BpuMI CCSGG 3 cut(s) 172, 202, 790
BsaAI YACGTR 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 828
BsaJI CCNNGG 7 cut(s) 86, 171, 260, 279, 310, 484, 516
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 170, 171, 266, 517
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 2 cut(s) 136, 228
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 3 cut(s) 261, 281, 300
BseDI CCNNGG 7 cut(s) 86, 171, 260, 279, 310, 484, 516
BseGI GGATG 3 cut(s) 271, 454, 492
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 5 cut(s) 119, 170, 171, 266, 517
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 405, 722
BseNI ACTGG 2 cut(s) 136, 228
BseSI GKGCMC 2 cut(s) 237, 895
BseXI GCAGC 7 cut(s) 296, 414, 551, 697, 783, 929, 938
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 85, 135
BshTI ACCGGT 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 4 cut(s) 113, 171, 201, 789
BslFI GGGAC 4 cut(s) 188, 262, 429, 840
BslI CCNNNNNNNGG 5 cut(s) 119, 170, 171, 266, 517
BsmFI GGGAC 4 cut(s) 188, 262, 429, 840
BsmI GAATGC 1 cut(s) 100
BsnI GGCC 2 cut(s) 85, 135
BsoBI CYCGRG 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 237, 895
Bsp143I GATC 5 cut(s) 104, 345, 370, 823, 829
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 4 cut(s) 186, 296, 431, 940
BspANI GGCC 2 cut(s) 85, 135
BspCNI CTCAG 2 cut(s) 404, 721
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 176, 856
BspPI GGATC 2 cut(s) 112, 378
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 2 cut(s) 136, 228
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 7 cut(s) 86, 171, 260, 279, 310, 484, 516
BssMI GATC 5 cut(s) 104, 345, 370, 823, 829
BssT1I CCWWGG 3 cut(s) 86, 484, 516
Bst2UI CCWGG 3 cut(s) 261, 281, 300
Bst4CI ACNGT 4 cut(s) 16, 311, 737, 890
Bst6I CTCTTC 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 896
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 391, 708
BstDSI CCRYGG 2 cut(s) 310, 484
BstF5I GGATG 3 cut(s) 271, 454, 492
BstFNI CGCG 1 cut(s) 574
BstHHI GCGC 2 cut(s) 576, 594
BstKTI GATC 5 cut(s) 107, 348, 373, 826, 832
BstMBI GATC 5 cut(s) 104, 345, 370, 823, 829
BstMWI GCNNNNNNNGC 3 cut(s) 562, 571, 694
BstNI CCWGG 3 cut(s) 261, 281, 300
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 6 cut(s) 170, 200, 259, 279, 298, 788
BstSLI GKGCMC 2 cut(s) 237, 895
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 7 cut(s) 296, 414, 551, 697, 783, 929, 938
BstX2I RGATCY 2 cut(s) 104, 823
BstYI RGATCY 2 cut(s) 104, 823
BsuRI GGCC 2 cut(s) 85, 135
BtgI CCRYGG 2 cut(s) 310, 484
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 3 cut(s) 271, 454, 492
BtsIMutI CAGTG 2 cut(s) 742, 895
Cac8I GCNNGC 1 cut(s) 338
CfoI GCGC 2 cut(s) 576, 594
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 6 cut(s) 83, 175, 293, 401, 455, 854
CseI GACGC 1 cut(s) 426
Csp6I GTAC 4 cut(s) 210, 481, 559, 811
CspAI ACCGGT 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 6 cut(s) 32, 485, 562, 566, 584, 880
CviQI GTAC 4 cut(s) 210, 481, 559, 811
DdeI CTNAG 2 cut(s) 391, 708
DpnI GATC 5 cut(s) 106, 347, 372, 825, 831
DpnII GATC 5 cut(s) 104, 345, 370, 823, 829
DraI TTTAAA 1 cut(s) 747
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
EciI GGCGGA 1 cut(s) 285
Eco130I CCWWGG 3 cut(s) 86, 484, 516
Eco47I GGWCC 5 cut(s) 175, 293, 401, 455, 854
Eco57I CTGAAG 2 cut(s) 681, 738
Eco72I CACGTG 1 cut(s) 896
Eco88I CYCGRG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 83
EcoRII CCWGG 3 cut(s) 259, 279, 298
EcoT14I CCWWGG 3 cut(s) 86, 484, 516
EcoT22I ATGCAT 1 cut(s) 567
ErhI CCWWGG 3 cut(s) 86, 484, 516
FaeI CATG 6 cut(s) 35, 488, 565, 569, 587, 883
FaqI GGGAC 4 cut(s) 188, 262, 429, 840
FatI CATG 6 cut(s) 31, 484, 561, 565, 583, 879
Fnu4HI GCNGC 8 cut(s) 285, 428, 431, 540, 686, 772, 927, 943
FokI GGATG 3 cut(s) 278, 461, 479
Fsp4HI GCNGC 8 cut(s) 285, 428, 431, 540, 686, 772, 927, 943
FspBI CTAG 2 cut(s) 543, 816
GlaI GCGC 2 cut(s) 575, 593
GluI GCNGC 8 cut(s) 285, 428, 431, 540, 686, 772, 927, 943
HaeIII GGCC 2 cut(s) 85, 135
HapII CCGG 4 cut(s) 113, 171, 201, 789
HgaI GACGC 1 cut(s) 426
HhaI GCGC 2 cut(s) 576, 594
Hin1II CATG 6 cut(s) 35, 488, 565, 569, 587, 883
Hin6I GCGC 2 cut(s) 574, 592
HinP1I GCGC 2 cut(s) 574, 592
HinfI GANTC 7 cut(s) 125, 330, 384, 489, 546, 569, 712
HpaII CCGG 4 cut(s) 113, 171, 201, 789
HphI GGTGA 2 cut(s) 175, 689
Hpy166II GTNNAC 2 cut(s) 249, 893
Hpy188I TCNGA 5 cut(s) 624, 711, 733, 796, 912
Hpy188III TCNNGA 3 cut(s) 414, 716, 827
Hpy8I GTNNAC 2 cut(s) 249, 893
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 3 cut(s) 29, 446, 656
HpyCH4III ACNGT 4 cut(s) 16, 311, 737, 890
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 9 cut(s) 10, 353, 565, 587, 727, 865, 893, 926, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 562, 571, 694
HpyF3I CTNAG 2 cut(s) 391, 708
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 6 cut(s) 35, 488, 565, 569, 587, 883
HspAI GCGC 2 cut(s) 574, 592
Kzo9I GATC 5 cut(s) 104, 345, 370, 823, 829
Lsp1109I GCAGC 7 cut(s) 296, 414, 551, 697, 783, 929, 938
MaeI CTAG 2 cut(s) 543, 816
MaeII ACGT 1 cut(s) 895
MalI GATC 5 cut(s) 106, 347, 372, 825, 831
MboI GATC 5 cut(s) 104, 345, 370, 823, 829
MboII GAAGA 1 cut(s) 379
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 2 cut(s) 104, 823
MhlI GDGCHC 2 cut(s) 237, 895
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 3 cut(s) 39, 288, 748
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 3 cut(s) 378, 540, 721
MmeI TCCRAC 2 cut(s) 756, 935
MnlI CCTC 5 cut(s) 414, 596, 790, 868, 959
Mox20I TGGCCA 1 cut(s) 135
Mph1103I ATGCAT 1 cut(s) 567
MscI TGGCCA 1 cut(s) 135
MseI TTAA 2 cut(s) 150, 746
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 3 cut(s) 433, 688, 942
MspI CCGG 4 cut(s) 113, 171, 201, 789
MspR9I CCNGG 6 cut(s) 172, 202, 261, 281, 300, 790
MunI CAATTG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 100
MvaI CCWGG 3 cut(s) 261, 281, 300
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 3 cut(s) 562, 571, 694
NciI CCSGG 3 cut(s) 172, 202, 790
NcoI CCATGG 1 cut(s) 484
NdeII GATC 5 cut(s) 104, 345, 370, 823, 829
NlaIII CATG 6 cut(s) 35, 488, 565, 569, 587, 883
NlaIV GGNNCC 2 cut(s) 176, 856
NsiI ATGCAT 1 cut(s) 567
NspI RCATGY 1 cut(s) 565
PctI GAATGC 1 cut(s) 100
PfeI GAWTC 4 cut(s) 125, 330, 489, 569
PinAI ACCGGT 1 cut(s) 112
PkrI GCNGC 8 cut(s) 286, 429, 432, 541, 687, 773, 928, 944
PleI GAGTC 3 cut(s) 378, 540, 720
PmaCI CACGTG 1 cut(s) 896
PmlI CACGTG 1 cut(s) 896
PpsI GAGTC 3 cut(s) 378, 540, 720
Ppu21I YACGTR 1 cut(s) 896
Psp6I CCWGG 3 cut(s) 259, 279, 298
PspCI CACGTG 1 cut(s) 896
PspGI CCWGG 3 cut(s) 259, 279, 298
PspN4I GGNNCC 2 cut(s) 176, 856
PspPI GGNCC 6 cut(s) 83, 175, 293, 401, 455, 854
PsuI RGATCY 2 cut(s) 104, 823
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 4 cut(s) 211, 482, 560, 812
RsaNI GTAC 4 cut(s) 210, 481, 559, 811
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 2 cut(s) 150, 746
SatI GCNGC 8 cut(s) 285, 428, 431, 540, 686, 772, 927, 943
Sau3AI GATC 5 cut(s) 104, 345, 370, 823, 829
Sau96I GGNCC 6 cut(s) 83, 175, 293, 401, 455, 854
SchI GAGTC 3 cut(s) 378, 540, 721
ScrFI CCNGG 6 cut(s) 172, 202, 261, 281, 300, 790
SduI GDGCHC 2 cut(s) 237, 895
SinI GGWCC 5 cut(s) 175, 293, 401, 455, 854
SmiMI CAYNNNNRTG 1 cut(s) 582
Sse9I AATT 3 cut(s) 39, 288, 748
SsiI CCGC 4 cut(s) 186, 296, 431, 940
SspMI CTAG 2 cut(s) 543, 816
StyD4I CCNGG 6 cut(s) 170, 200, 259, 279, 298, 788
StyI CCWWGG 3 cut(s) 86, 484, 516
TaaI ACNGT 4 cut(s) 16, 311, 737, 890
TaiI ACGT 1 cut(s) 898
TaqI TCGA 1 cut(s) 140
TaqII GACCGA 1 cut(s) 842
TasI AATT 3 cut(s) 39, 288, 748
TatI WGTACW 1 cut(s) 810
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 4 cut(s) 125, 330, 489, 569
Tru1I TTAA 2 cut(s) 150, 746
Tru9I TTAA 2 cut(s) 150, 746
TscAI CASTG 2 cut(s) 742, 895
TseI GCWGC 7 cut(s) 284, 427, 539, 685, 771, 926, 942
TspDTI ATGAA 5 cut(s) 48, 57, 465, 582, 653
TspGWI ACGGA 3 cut(s) 461, 485, 539
TspRI CASTG 2 cut(s) 742, 895
VneI GTGCAC 1 cut(s) 891
VpaK11BI GGWCC 5 cut(s) 175, 293, 401, 455, 854
XapI RAATTY 1 cut(s) 748
XceI RCATGY 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XspI CTAG 2 cut(s) 543, 816
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.