pycom14g03970

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012069
Physical Location & Seq
Reverse (-)
3436419 .. 3438037
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGGAACAAAACCTCTCCGCCCAAACCCTCCCCAACTCACTCCCCAACCACCGGAACGTCGCCTCAGACGACGACGACGATGCACCGACCCTAAGCCTTCAAGCCCTAGAAGCTCTGAAGGAGTTCCTAGGCGAGCAAACCCAGTCATCACCCGACACCGCCGCCGAGAGCCGAGAAGCTGATTCGACTGGTCCTGCTCTGATCGCCGAAGACTGGAGACTGAGTCAGTTCTGGTATGACCCCCGGACCGCCCAAACCCTAGCCCAAGAGGTTCTCACTCTCTGCAACTCCGCCACCGCCTCCGCTCGGGTCGCCTGCGTGGCTTGCCCCTCACTCTACGCCTATCTCAAGAATGCTGATTCCGATGTGTCTGTACAACTGCTCGAGTATGACAAGCGGTTTGAGCAGTACGGCAGTGATTTCTCGTTCTACGATTACAACCGGCCTGAAGATTTGCCATTGGAGCTGAAGAATGCTTTTGACATTGTGGTTGCTGATCCTCCTTACCTGAGTCAGGAGTGCTTGGAGAAAGTCGCTCAAACAATTTCATTTCTTGCACGATCAGAAAAATCTTACTTGCTTCTCCTCACAGGAGCTGTGCAGAAAGAGAGAGCAGCTGAACTCATGGGATTGCATCCATGTGGTTTTAGGCCTCAACACTCTAGCAAACTAGGAAACGAGTTTCGTCTGTTCACAAACTATGATCCTGAGACGAGATTAGAAGGGTGGGAGTTAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N6-adenineMlase

Protein Analysis

248

Amino Acids

27.7

Weight (kDa)

4.46

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N6-adenineMlase PF10237 70 - 235 1.5e-57 Probable N6-adenine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58470
fragaria_vesca FvH4_6g21270
malus_domestica MD14G1048200.v1.1
prunus_persica Prupe.7G087000_v2.0.a1
pyrus_communis pycom14g03970
rosa_chinensis RchiOBHm_Chr3g0477741 RchiOBHm_Chr3g0477781
rosa_laevigata RLG00000023692
rosa_multiflora Rmu_sc0017665.1_g000001 Rmu_sc0026290.1_g000001
rosa_roxburghii Rroxscaffold_6G00403710
rosa_rugosa Rorug03G0162900 Rorug03G0163000 Rorug03G0163100
rosa_samantha Rh3AG213400 Rh3BG247200 Rh3CG241000 Rh3DG240300
rosa_wichuraiana Rw3G019310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 305
AciI CCGC 8 cut(s) 18, 159, 162, 249, 291, 297, 303, 397
AclWI GGATC 2 cut(s) 491, 698
AcuI CTGAAG 3 cut(s) 137, 468, 488
AfaI GTAC 2 cut(s) 375, 410
AfiI CCNNNNNNNGG 4 cut(s) 51, 213, 306, 514
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 5 cut(s) 113, 179, 466, 596, 617
AluI AGCT 5 cut(s) 113, 179, 466, 596, 617
Alw26I GTCTC 2 cut(s) 211, 704
AlwI GGATC 2 cut(s) 491, 698
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 2 cut(s) 306, 383
AoxI GGCC 2 cut(s) 443, 650
ApeKI GCWGC 1 cut(s) 614
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
Asp700I GAANNNNTTC 1 cut(s) 122
AspA2I CCTAGG 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 191, 246
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 141
AvaI CYCGRG 2 cut(s) 306, 383
AvaII GGWCC 2 cut(s) 191, 246
AvrII CCTAGG 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 626
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 211, 704
BfaI CTAG 5 cut(s) 107, 128, 260, 663, 671
BglI GCCNNNNNGGC 1 cut(s) 320
BisI GCNGC 2 cut(s) 162, 615
BlnI CCTAGG 1 cut(s) 127
BlsI GCNGC 2 cut(s) 163, 616
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 2 cut(s) 191, 246
BmeT110I CYCGRG 2 cut(s) 306, 383
BmgT120I GGNCC 2 cut(s) 191, 246
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 2 cut(s) 70, 643
BmuI ACTGGG 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 235
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 127, 242
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 213, 306, 514
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 3 cut(s) 142, 193, 218
BseDI CCNNGG 2 cut(s) 127, 242
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 4 cut(s) 51, 213, 306, 514
BseMII CTCAG 4 cut(s) 78, 212, 500, 699
BseNI ACTGG 3 cut(s) 142, 193, 218
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 626
BsgI GTGCAG 1 cut(s) 620
BshFI GGCC 2 cut(s) 445, 652
BsiHKCI CYCGRG 2 cut(s) 306, 383
BsiSI CCGG 3 cut(s) 52, 244, 442
BslI CCNNNNNNNGG 4 cut(s) 51, 213, 306, 514
BsmAI GTCTC 2 cut(s) 211, 704
BsmBI CGTCTC 1 cut(s) 704
BsmI GAATGC 2 cut(s) 358, 478
BsnI GGCC 2 cut(s) 445, 652
BsoBI CYCGRG 2 cut(s) 306, 383
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 201, 496, 560, 703
BspACI CCGC 8 cut(s) 18, 159, 162, 249, 291, 297, 303, 397
BspANI GGCC 2 cut(s) 445, 652
BspCNI CTCAG 4 cut(s) 77, 213, 501, 700
BspPI GGATC 2 cut(s) 491, 698
BsrBI CCGCTC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 441
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 3 cut(s) 142, 193, 218
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 2 cut(s) 127, 242
BssMI GATC 4 cut(s) 201, 496, 560, 703
BssT1I CCWWGG 1 cut(s) 127
BstAUI TGTACA 1 cut(s) 373
BstC8I GCNNGC 3 cut(s) 134, 316, 325
BstDEI CTNAG 5 cut(s) 64, 92, 221, 509, 708
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 1 cut(s) 634
BstKTI GATC 4 cut(s) 204, 499, 563, 706
BstMAI GTCTC 2 cut(s) 211, 704
BstMBI GATC 4 cut(s) 201, 496, 560, 703
BstMWI GCNNNNNNNGC 7 cut(s) 110, 203, 311, 320, 324, 403, 463
BstSCI CCNGG 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 626
BstV2I GAAGAC 1 cut(s) 216
BsuRI GGCC 2 cut(s) 445, 652
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 3 cut(s) 134, 316, 325
CaiI CAGNNNCTG 1 cut(s) 596
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 2 cut(s) 191, 246
CpoI CGGWCCG 1 cut(s) 246
Csp6I GTAC 2 cut(s) 374, 409
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 2 cut(s) 625, 639
CviQI GTAC 2 cut(s) 374, 409
DdeI CTNAG 5 cut(s) 64, 92, 221, 509, 708
DpnI GATC 4 cut(s) 203, 498, 562, 705
DpnII GATC 4 cut(s) 201, 496, 560, 703
EciI GGCGGA 2 cut(s) 7, 280
Eco130I CCWWGG 1 cut(s) 127
Eco147I AGGCCT 1 cut(s) 652
Eco47I GGWCC 2 cut(s) 191, 246
Eco57I CTGAAG 3 cut(s) 137, 468, 488
Eco88I CYCGRG 2 cut(s) 306, 383
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoT14I CCWWGG 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 2 cut(s) 628, 642
FaiI YATR 5 cut(s) 237, 390, 626, 640, 702
FatI CATG 2 cut(s) 624, 638
Fnu4HI GCNGC 2 cut(s) 162, 615
FokI GGATG 1 cut(s) 621
Fsp4HI GCNGC 2 cut(s) 162, 615
FspBI CTAG 5 cut(s) 107, 128, 260, 663, 671
GluI GCNGC 2 cut(s) 162, 615
GsuI CTGGAG 1 cut(s) 235
HaeIII GGCC 2 cut(s) 445, 652
HapII CCGG 3 cut(s) 52, 244, 442
Hin1II CATG 2 cut(s) 628, 642
HinfI GANTC 4 cut(s) 182, 223, 359, 511
HpaII CCGG 3 cut(s) 52, 244, 442
HphI GGTGA 1 cut(s) 141
Hpy166II GTNNAC 1 cut(s) 693
Hpy188I TCNGA 5 cut(s) 67, 117, 201, 364, 565
Hpy188III TCNNGA 3 cut(s) 349, 515, 707
Hpy8I GTNNAC 1 cut(s) 693
Hpy99I CGWCG 4 cut(s) 62, 74, 77, 80
HpyAV CCTTC 3 cut(s) 107, 112, 716
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 5 cut(s) 83, 285, 557, 601, 634
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 203, 311, 320, 324, 403, 463
HpyF3I CTNAG 5 cut(s) 64, 92, 221, 509, 708
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 2 cut(s) 628, 642
Kzo9I GATC 4 cut(s) 201, 496, 560, 703
LmnI GCTCC 2 cut(s) 463, 593
Lsp1109I GCAGC 1 cut(s) 626
LweI GCATC 2 cut(s) 70, 643
MaeI CTAG 5 cut(s) 107, 128, 260, 663, 671
MaeII ACGT 1 cut(s) 57
MalI GATC 4 cut(s) 203, 498, 562, 705
MbiI CCGCTC 1 cut(s) 305
MboI GATC 4 cut(s) 201, 496, 560, 703
MboII GAAGA 3 cut(s) 221, 461, 481
MluCI AATT 1 cut(s) 543
MlyI GAGTC 2 cut(s) 232, 520
MnlI CCTC 9 cut(s) 23, 38, 73, 262, 310, 340, 510, 596, 663
MroXI GAANNNNTTC 1 cut(s) 122
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 3 cut(s) 52, 244, 442
MspR9I CCNGG 1 cut(s) 244
Mva1269I GAATGC 2 cut(s) 358, 478
MwoI GCNNNNNNNGC 7 cut(s) 110, 203, 311, 320, 324, 403, 463
NciI CCSGG 1 cut(s) 244
NdeII GATC 4 cut(s) 201, 496, 560, 703
NlaIII CATG 2 cut(s) 628, 642
NmeAIII GCCGAG 2 cut(s) 190, 197
PaeR7I CTCGAG 1 cut(s) 383
PceI AGGCCT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 75
PctI GAATGC 2 cut(s) 358, 478
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 182, 359
PflFI GACNNNGTC 1 cut(s) 222
PkrI GCNGC 2 cut(s) 163, 616
PleI GAGTC 2 cut(s) 231, 519
PpsI GAGTC 2 cut(s) 231, 519
PspPI GGNCC 2 cut(s) 191, 246
PspXI VCTCGAGB 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 596
PsyI GACNNNGTC 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 375, 410
RsaNI GTAC 2 cut(s) 374, 409
RseI CAYNNNNRTG 1 cut(s) 639
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SatI GCNGC 2 cut(s) 162, 615
Sau3AI GATC 4 cut(s) 201, 496, 560, 703
Sau96I GGNCC 2 cut(s) 191, 246
SchI GAGTC 2 cut(s) 232, 520
ScrFI CCNGG 1 cut(s) 244
SetI ASST 9 cut(s) 15, 60, 115, 181, 273, 468, 510, 598, 619
SfaNI GCATC 2 cut(s) 70, 643
Sfr274I CTCGAG 1 cut(s) 383
SinI GGWCC 2 cut(s) 191, 246
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 347, 383
SmoI CTYRAG 2 cut(s) 347, 383
Sse9I AATT 1 cut(s) 543
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 8 cut(s) 18, 159, 162, 249, 291, 297, 303, 397
SspMI CTAG 5 cut(s) 107, 128, 260, 663, 671
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 127
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 185, 384
TasI AATT 1 cut(s) 543
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 182, 359
TscAI CASTG 1 cut(s) 421
TseI GCWGC 1 cut(s) 614
TspDTI ATGAA 1 cut(s) 537
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 222
VpaK11BI GGWCC 2 cut(s) 191, 246
XagI CCTNNNNNAGG 1 cut(s) 512
XhoI CTCGAG 1 cut(s) 383
XmaJI CCTAGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 5 cut(s) 107, 128, 260, 663, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.