pycom14g04210

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007318
Physical Location & Seq
Forward (+)
3647231 .. 3650757
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGAACCGGCTCGCACCGTTGTCGGAAGAGCCGAACATCAACGGAGAAGAGGACGCCGCGAACCGGTCGAGCAAAGGCATTCTGCGGAGCACTCAGCCGTGGCGGAACTGGATCAAGACCCACCTTTCTCCTCTCGTGCTCTTCCACAAGAGGTCCGACTTCAATGTGCTTCTCAGTGTTCTTGGTTGCCCTCTCTTCCCTGTCTCTGCCCTCCCCAAACTACCCCTCAATGAGGTTTCCTCAACTGCACAATACATAATACAACACTTCACAGCTGCCACCGGTTGCCGCAAGCTAGAAGGCAAAGTGAAGAACATTTTCGCAACTGGGAAAGTGACGATGGCAATGGTTGATGATCCCGGCCCCGGTGGTGGCTCGGCTGCCGGAACCACCGCAGTTTCGGAAAAAGGGTGCTTTGTGATGTGGCAGATGGTTCCTAACAAGTGGCTGATTGAGCTAGTTCTAGGTGGTCACAAGGTGGCAGCTGGTAGCGATGGCTTAGTCGCTTGGCGTCACACGCCGTGGCTAGGTGTCCACGCTGCCAAGGGCCGCATTCGTCCACTCCGGCGAGCTCTTCAGGGATTAGATCCAATGGCAATATCAGCTGTATTTTCCCCAGCGCAGTACGTGGGGGAAAAGCAAATCTCCGGCGTTGATTGCTTCGTCTTGAAATTGTCAGCTGATCAAACGGATCTGGCTGAACGAAGTGATAGCACAGCAGAGATGATCAAACATGTAATATTTGGCTACTTCAGCCAAAGAAATGGGCTGCTAGTGTACCTAGAGGACTCTTATTTAACCAGGATCCAGTCACCTGGTGCCTACCCTACCTACTGGGAGACCACAATGTCGACGAAAATCGAGGACTATCGGACGGTGGAGGGTGTGATGATCGCACACGCCGGTCAGACGAATGTGATCATCACACGGTTTGGGGACAATCTAAAGACAGGGTCAGCGATCACGCGGCTGGAAGAAACATGGACGATTGATGATGTTGCATTCAACGTCCCTGGATTGTCCATGGATTGCTTCATTCCTCCAAAAGAAGTACAGAAAGATTACCCTAAGAAGAATTTTGATTGGAGATCACCATTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

368

Amino Acids

40.54

Weight (kDa)

8.48

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 106 - 348 1.9e-105 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 847
AccB1I GGYRCC 1 cut(s) 818
AccI GTMKAC 1 cut(s) 851
AccII CGCG 2 cut(s) 59, 967
AciI CCGC 7 cut(s) 57, 85, 103, 289, 393, 550, 967
AclWI GGATC 6 cut(s) 119, 350, 581, 699, 799, 812
AcsI RAATTY 1 cut(s) 1075
AcuI CTGAAG 2 cut(s) 560, 736
AcyI GRCGYC 2 cut(s) 54, 511
AdeI CACNNNGTG 3 cut(s) 478, 522, 818
AfaI GTAC 3 cut(s) 626, 779, 1053
AfiI CCNNNNNNNGG 5 cut(s) 63, 232, 365, 371, 527
AflIII ACRYGT 1 cut(s) 733
AgeI ACCGGT 2 cut(s) 63, 281
AgsI TTSAA 3 cut(s) 163, 670, 1006
AjnI CCWGG 3 cut(s) 800, 814, 1012
AluBI AGCT 7 cut(s) 275, 295, 457, 485, 572, 605, 680
AluI AGCT 7 cut(s) 275, 295, 457, 485, 572, 605, 680
Alw21I GWGCWC 3 cut(s) 92, 141, 574
Alw26I GTCTC 2 cut(s) 208, 833
AlwI GGATC 6 cut(s) 119, 350, 581, 699, 799, 812
AoxI GGCC 2 cut(s) 361, 547
ApeKI GCWGC 5 cut(s) 275, 380, 482, 539, 769
ApoI RAATTY 1 cut(s) 1075
AsiGI ACCGGT 2 cut(s) 63, 281
Asp700I GAANNNNTTC 1 cut(s) 317
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 3 cut(s) 153, 362, 547
AsuC2I CCSGG 2 cut(s) 360, 366
AsuHPI GGTGA 2 cut(s) 804, 1083
AvaII GGWCC 1 cut(s) 153
BamHI GGATCC 1 cut(s) 804
BanI GGYRCC 1 cut(s) 818
BanII GRGCYC 1 cut(s) 574
BauI CACGAG 1 cut(s) 134
Bbv12I GWGCWC 3 cut(s) 92, 141, 574
BbvI GCAGC 5 cut(s) 262, 367, 494, 526, 756
BccI CCATC 3 cut(s) 334, 424, 488
BceAI ACGGC 2 cut(s) 82, 505
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 802, 816, 1014
BclI TGATCA 3 cut(s) 682, 726, 918
BcnI CCSGG 2 cut(s) 360, 366
BcoDI GTCTC 2 cut(s) 208, 833
BfaI CTAG 6 cut(s) 296, 458, 464, 527, 773, 782
BisI GCNGC 9 cut(s) 57, 276, 289, 381, 483, 540, 550, 770, 968
BlsI GCNGC 9 cut(s) 58, 277, 290, 382, 484, 541, 551, 771, 969
Bme1390I CCNGG 5 cut(s) 360, 366, 802, 816, 1014
Bme18I GGWCC 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 153, 362, 547
BmiI GGNNCC 5 cut(s) 364, 388, 435, 806, 820
BmrFI CCNGG 5 cut(s) 360, 366, 802, 816, 1014
BmrI ACTGGG 2 cut(s) 336, 844
BmuI ACTGGG 2 cut(s) 336, 844
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpuMI CCSGG 2 cut(s) 360, 366
BsaAI YACGTR 1 cut(s) 628
BsaHI GRCGYC 2 cut(s) 54, 511
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 6 cut(s) 98, 364, 521, 543, 1012, 1023
BsaWI WCCGGW 2 cut(s) 63, 281
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 232, 365, 371, 527
Bse118I RCCGGY 4 cut(s) 6, 63, 281, 902
Bse1I ACTGG 4 cut(s) 113, 331, 808, 839
Bse3DI GCAATG 2 cut(s) 351, 1094
BseBI CCWGG 3 cut(s) 802, 816, 1014
BseDI CCNNGG 6 cut(s) 98, 364, 521, 543, 1012, 1023
BseLI CCNNNNNNNGG 5 cut(s) 63, 232, 365, 371, 527
BseMI GCAATG 2 cut(s) 351, 1094
BseMII CTCAG 2 cut(s) 107, 187
BseNI ACTGG 4 cut(s) 113, 331, 808, 839
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 5 cut(s) 262, 367, 494, 526, 756
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 59, 967
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 363, 549
BshNI GGYRCC 1 cut(s) 818
BshTI ACCGGT 2 cut(s) 63, 281
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 3 cut(s) 92, 141, 574
BsiSI CCGG 9 cut(s) 7, 64, 282, 360, 366, 384, 565, 648, 903
BslFI GGGAC 2 cut(s) 950, 995
BslI CCNNNNNNNGG 5 cut(s) 63, 232, 365, 371, 527
BsmAI GTCTC 2 cut(s) 208, 833
BsmFI GGGAC 2 cut(s) 950, 995
BsmI GAATGC 3 cut(s) 78, 552, 1001
BsnI GGCC 2 cut(s) 363, 549
Bso31I GGTCTC 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 92, 141, 574
Bsp19I CCATGG 1 cut(s) 1023
BspACI CCGC 7 cut(s) 57, 85, 103, 289, 393, 550, 967
BspANI GGCC 2 cut(s) 363, 549
BspCNI CTCAG 2 cut(s) 106, 186
BspFNI CGCG 2 cut(s) 59, 967
BspLI GGNNCC 5 cut(s) 364, 388, 435, 806, 820
BspPI GGATC 6 cut(s) 119, 350, 581, 699, 799, 812
BspQI GCTCTTC 3 cut(s) 21, 146, 579
BspT107I GGYRCC 1 cut(s) 818
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 351, 1094
BsrFI RCCGGY 4 cut(s) 6, 63, 281, 902
BsrI ACTGG 4 cut(s) 113, 331, 808, 839
BssAI RCCGGY 4 cut(s) 6, 63, 281, 902
BssECI CCNNGG 6 cut(s) 98, 364, 521, 543, 1012, 1023
BssNI GRCGYC 2 cut(s) 54, 511
BssSI CACGAG 1 cut(s) 134
BssT1I CCWWGG 2 cut(s) 543, 1023
Bst2BI CACGAG 1 cut(s) 134
Bst2UI CCWGG 3 cut(s) 802, 816, 1014
Bst4CI ACNGT 3 cut(s) 18, 877, 930
Bst6I CTCTTC 5 cut(s) 21, 42, 146, 200, 579
BstACI GRCGYC 2 cut(s) 54, 511
BstBAI YACGTR 1 cut(s) 628
BstC8I GCNNGC 3 cut(s) 12, 293, 570
BstDEI CTNAG 4 cut(s) 93, 173, 499, 1068
BstDSI CCRYGG 3 cut(s) 98, 521, 1023
BstENI CCTNNNNNAGG 1 cut(s) 230
BstFNI CGCG 2 cut(s) 59, 967
BstHHI GCGC 1 cut(s) 622
BstMAI GTCTC 2 cut(s) 208, 833
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 5 cut(s) 454, 517, 602, 657, 753
BstNI CCWGG 3 cut(s) 802, 816, 1014
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 5 cut(s) 358, 364, 800, 814, 1012
BstUI CGCG 2 cut(s) 59, 967
BstV1I GCAGC 5 cut(s) 262, 367, 494, 526, 756
BstX2I RGATCY 3 cut(s) 586, 691, 804
BstXI CCANNNNNNTGG 2 cut(s) 764, 815
BstYI RGATCY 3 cut(s) 586, 691, 804
BsuRI GGCC 2 cut(s) 363, 549
BtgI CCRYGG 3 cut(s) 98, 521, 1023
BtgZI GCGATG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 3 cut(s) 12, 293, 570
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 4 cut(s) 6, 63, 281, 902
Cfr13I GGNCC 3 cut(s) 153, 362, 547
CseI GACGC 2 cut(s) 62, 500
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 3 cut(s) 625, 778, 1052
CspAI ACCGGT 2 cut(s) 63, 281
CviAII CATG 3 cut(s) 734, 981, 1024
CviQI GTAC 3 cut(s) 625, 778, 1052
DdeI CTNAG 4 cut(s) 93, 173, 499, 1068
DraIII CACNNNGTG 3 cut(s) 478, 522, 818
DrdI GACNNNNNNGTC 1 cut(s) 847
DseDI GACNNNNNNGTC 1 cut(s) 847
Eam1104I CTCTTC 5 cut(s) 21, 42, 146, 200, 579
EarI CTCTTC 5 cut(s) 21, 42, 146, 200, 579
EciI GGCGGA 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 572
Eco130I CCWWGG 2 cut(s) 543, 1023
Eco24I GRGCYC 1 cut(s) 574
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 560, 736
EcoICRI GAGCTC 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 800, 814, 1012
EcoT14I CCWWGG 2 cut(s) 543, 1023
EcoT38I GRGCYC 1 cut(s) 574
ErhI CCWWGG 2 cut(s) 543, 1023
FaeI CATG 3 cut(s) 737, 984, 1027
FaiI YATR 4 cut(s) 257, 735, 982, 1025
FaqI GGGAC 2 cut(s) 950, 995
FatI CATG 3 cut(s) 733, 980, 1023
FbaI TGATCA 3 cut(s) 682, 726, 918
FblI GTMKAC 1 cut(s) 851
Fnu4HI GCNGC 9 cut(s) 57, 276, 289, 381, 483, 540, 550, 770, 968
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 9 cut(s) 57, 276, 289, 381, 483, 540, 550, 770, 968
FspBI CTAG 6 cut(s) 296, 458, 464, 527, 773, 782
GlaI GCGC 1 cut(s) 621
GluI GCNGC 9 cut(s) 57, 276, 289, 381, 483, 540, 550, 770, 968
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 2 cut(s) 363, 549
HapII CCGG 9 cut(s) 7, 64, 282, 360, 366, 384, 565, 648, 903
HgaI GACGC 2 cut(s) 62, 500
HhaI GCGC 1 cut(s) 622
Hin1I GRCGYC 2 cut(s) 54, 511
Hin1II CATG 3 cut(s) 737, 984, 1027
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HinfI GANTC 1 cut(s) 788
HpaII CCGG 9 cut(s) 7, 64, 282, 360, 366, 384, 565, 648, 903
HphI GGTGA 2 cut(s) 804, 1083
Hpy166II GTNNAC 4 cut(s) 535, 560, 778, 852
Hpy188I TCNGA 5 cut(s) 25, 157, 403, 873, 909
Hpy188III TCNNGA 2 cut(s) 115, 667
Hpy8I GTNNAC 4 cut(s) 535, 560, 778, 852
Hpy99I CGWCG 1 cut(s) 856
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 3 cut(s) 18, 877, 930
HpyCH4IV ACGT 2 cut(s) 627, 1008
HpyCH4V TGCA 3 cut(s) 248, 1001, 1099
HpyF10VI GCNNNNNNNGC 5 cut(s) 454, 517, 602, 657, 753
HpyF3I CTNAG 4 cut(s) 93, 173, 499, 1068
HpySE526I ACGT 2 cut(s) 627, 1008
Hsp92I GRCGYC 2 cut(s) 54, 511
Hsp92II CATG 3 cut(s) 737, 984, 1027
HspAI GCGC 1 cut(s) 620
Ksp22I TGATCA 3 cut(s) 682, 726, 918
LguI GCTCTTC 3 cut(s) 21, 146, 579
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 5 cut(s) 262, 367, 494, 526, 756
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 6 cut(s) 296, 458, 464, 527, 773, 782
MaeII ACGT 2 cut(s) 627, 1008
MaeIII GTNAC 4 cut(s) 334, 470, 512, 810
MboII GAAGA 8 cut(s) 38, 59, 133, 187, 322, 566, 986, 1084
MflI RGATCY 3 cut(s) 586, 691, 804
MhlI GDGCHC 3 cut(s) 92, 141, 574
MluCI AATT 2 cut(s) 671, 1075
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 317
MseI TTAA 2 cut(s) 797, 1102
MspA1I CMGCKG 4 cut(s) 275, 485, 605, 680
MspI CCGG 9 cut(s) 7, 64, 282, 360, 366, 384, 565, 648, 903
MspR9I CCNGG 5 cut(s) 360, 366, 802, 816, 1014
Mva1269I GAATGC 3 cut(s) 78, 552, 1001
MvaI CCWGG 3 cut(s) 802, 816, 1014
MvnI CGCG 2 cut(s) 59, 967
MwoI GCNNNNNNNGC 5 cut(s) 454, 517, 602, 657, 753
NciI CCSGG 2 cut(s) 360, 366
NcoI CCATGG 1 cut(s) 1023
NlaIII CATG 3 cut(s) 737, 984, 1027
NlaIV GGNNCC 5 cut(s) 364, 388, 435, 806, 820
NmeAIII GCCGAG 1 cut(s) 356
NmuCI GTSAC 4 cut(s) 334, 470, 512, 810
NspI RCATGY 1 cut(s) 737
PciI ACATGT 1 cut(s) 733
PciSI GCTCTTC 3 cut(s) 21, 146, 579
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 3 cut(s) 78, 552, 1001
PdmI GAANNNNTTC 1 cut(s) 317
PflFI GACNNNGTC 1 cut(s) 952
PinAI ACCGGT 2 cut(s) 63, 281
PkrI GCNGC 9 cut(s) 58, 277, 290, 382, 484, 541, 551, 771, 969
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
Ppu21I YACGTR 1 cut(s) 628
PscI ACATGT 1 cut(s) 733
Psp124BI GAGCTC 1 cut(s) 574
Psp6I CCWGG 3 cut(s) 800, 814, 1012
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 3 cut(s) 800, 814, 1012
PspN4I GGNNCC 5 cut(s) 364, 388, 435, 806, 820
PspPI GGNCC 3 cut(s) 153, 362, 547
PsuI RGATCY 3 cut(s) 586, 691, 804
PsyI GACNNNGTC 1 cut(s) 952
PvuII CAGCTG 4 cut(s) 275, 485, 605, 680
RsaI GTAC 3 cut(s) 626, 779, 1053
RsaNI GTAC 3 cut(s) 625, 778, 1052
SacI GAGCTC 1 cut(s) 574
SalI GTCGAC 1 cut(s) 850
SapI GCTCTTC 3 cut(s) 21, 146, 579
SaqAI TTAA 2 cut(s) 797, 1102
SatI GCNGC 9 cut(s) 57, 276, 289, 381, 483, 540, 550, 770, 968
Sau96I GGNCC 3 cut(s) 153, 362, 547
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 5 cut(s) 360, 366, 802, 816, 1014
SduI GDGCHC 3 cut(s) 92, 141, 574
SexAI ACCWGGT 1 cut(s) 814
SinI GGWCC 1 cut(s) 153
Sse9I AATT 2 cut(s) 671, 1075
SsiI CCGC 7 cut(s) 57, 85, 103, 289, 393, 550, 967
SspI AATATT 1 cut(s) 741
SspMI CTAG 6 cut(s) 296, 458, 464, 527, 773, 782
SstI GAGCTC 1 cut(s) 574
StyD4I CCNGG 5 cut(s) 358, 364, 800, 814, 1012
StyI CCWWGG 2 cut(s) 543, 1023
TaaI ACNGT 3 cut(s) 18, 877, 930
TaiI ACGT 2 cut(s) 630, 1011
TaqI TCGA 3 cut(s) 68, 851, 861
TasI AATT 2 cut(s) 671, 1075
TatI WGTACW 1 cut(s) 1051
TauI GCSGC 4 cut(s) 59, 291, 552, 970
Tru1I TTAA 2 cut(s) 797, 1102
Tru9I TTAA 2 cut(s) 797, 1102
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 4 cut(s) 334, 470, 512, 810
TseI GCWGC 5 cut(s) 275, 380, 482, 539, 769
Tsp45I GTSAC 4 cut(s) 334, 470, 512, 810
TspDTI ATGAA 2 cut(s) 17, 1024
TspGWI ACGGA 2 cut(s) 57, 704
TspRI CASTG 1 cut(s) 181
Tth111I GACNNNGTC 1 cut(s) 952
VpaK11BI GGWCC 1 cut(s) 153
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 1 cut(s) 1075
XceI RCATGY 1 cut(s) 737
XmiI GTMKAC 1 cut(s) 851
XmnI GAANNNNTTC 1 cut(s) 317
XspI CTAG 6 cut(s) 296, 458, 464, 527, 773, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.