pycom14g05290
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006756
Physical Location & Seq
Reverse (-)
5059694 .. 5062932
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2655 bp
ATGGCTACTTCCTGCAAACATCCTTTACTTCTCTCTCTAATAATCTCCACATTTTTCCTTCTCACTTCAGCATTATTATCAGAACCAGACATTCTTCTCACCTTCAAGGCCTCCATTAAAGATCCCCAAAACTCACTCTCAACCTGGTCCAACAACTCGGGCACCCACTACTGCCAATGGCCTGGAATCACCTGCACTGCCACCACCACCACCACACTCTCCGTCACCTCCCTCAACCTCCAAAGCTTCAATCTTTCAGGTGACATCTCACCCACAATCTGTGGGCTCCCTTATCTGTCAGAGCTCAACCTTGCAGACAACTTCTTCAACCAGCCCATCCCTCTCCACCTCTCTCAGTGCACTTCTCTGGAGACTTTGAATCTCAGCAACAATTTAATCTGGGGTACAATCTTGAACCAGATTTCTCAGTTTGGTAACTTGAAAGTTCTCGACTTGAGCAAAAACCGTGTTGAAGGAAACATCCCACAAGCCATTGCTGCACTCCACAGCCTCCAGGTTCTCAACTTGGGAAGCAACTTGCTTACGGGTAGTGTGCCCTCTATTTTTGCGAATTTGACTGAGCTAGTTGTTCTTGATCTGTCTCAAAATACATACTTGGTGAGTGAGATTCCTACCGACATTGGGAAGCTTGTGAAGCTTGAGAAGTTGTTCTTGCAAAGCTCTGGTTTTCATGGTGTAATTCCTAATTCCTTGGTGGGTTTACAAAGTTTGAGTGCTTTGGACTTTTCCCAAAATAACTTAACTGGTAGGGTTCCTCAGATACTAGGGTTTTCTCTCAAAAACTTGGTGTCCTTTGATGTTTCAGAGAATAGGCTTTCTGGGTTTTTCCCAAATGGCATCTGTAACGGAAAATACCTTATATACCTCAGCCTTCACACAAATGTGTTCAATGGTACAATACCTAACTCCATTAGTGAATGCTCAAGGCTTGAGGTGTTTGAAGTTCAAAACAATTTGTTTTCTGGGGATTTCCCAGTTGGGCTTTGGTCATTGCCCAAAATTAAGCTCTTAAGAGCTGAGAATAATCGGTTTTCGGGAGAAATACCTGATTCAGTATCAATGGCTGGTCAGTTGGAGCAGGTTCAAATAGATAACAACAGCTTCACTAGCAAAATTCCTCATGGTCTTGGGTTAGTCAAGAGTTTATATAGATTCTCTGCTTCTCTTAATGGTTTATATGGTGAACTCCCAACAAACTTCTGTGACTCCCCTGTTATGAGTATAGTGAATCTCTCCCACAATGCGCTCTCGGGTCAAATTCCTGAGGTTAAGAAATGCAGGAAGCTAGTGTCATTGTCTTTGGCAGACAACAATCTTAGTGGGAGTATCCCAGAATCTCTTGGTGAATTACCAGTGCTAACTTACCTTGATCTTTCTGACAATAAGCTAACCGGTCCCCTTCCACAGGCGCTTCAGAACTTGAAGCTTGCACTCTTCAATGTTTCCTTTAATCAGCTATCTGGTAGAGTCCCCTATTCTCTTATCGCAGGCCTCCCAGCTTCATTTCTTCAAGGAAATCCTGACCTTTGCGGTCCAGGATTACTCGACTCTTGTTCTGATGACCAGCCAAGACGCCATTCTTCTGATCTTACGACATTGACAAGTGCCTTGATATCTTTAGCTTTTGCTGTTGGAACCCTGATTATTGCTGGTGGGTTTATTTTTTATCATCGATATTATAAGCAGCGAGCTCAAGTCGGCCTTTGGCGTTCGGTGTTCTTTTATCCTCTCAGAGTCACTGAGCATGACTTGATGATGGGAATGGATGAGAAATCTGCTGCAGGGTGTGGTGGGGTGTTTGGTAGAGTTTACATTGTAAGTTTACCAAGTGGTGAACTTGTTGCTGTGAAGAAGCTTGTGAATTTCGGGATCCAGTCTTCGAAAACTTTGAAGACTGAGATCAAGACCTTGGCCAAGATAAGGCATAAGAACATTGTAAAAGTTCTGGGATTCTGCTATTCTGATGATTCAATATTTCTGATATATGAGTTCCAACAGAAGGGGAGCTTGGGGGACTTGATCTGCAGACCTGATTTTAGTTTGCAGTGGAGTGTTAGATTGAGAATTGCCATTGGCATTGCACAAGGACTGGCATACCTTCACAAGGATTATGTTCCTCATTTGCTTCATAGAAATCTCAAGTCAAACAATATCCTTCTGGATGCAGAGTTTGAACCAAAGCTCACAGATTTTGCTCTCGACAGAATTGTGGGAGAAGCTGCCTTTCAATCAACCTTGGCTTCGGAATCTGCTTTATCCTGTTACAATGCGCCAGAATGCAAGTACAGTAAGAAAGCAACTGAACAAATGGATGTTTACAGCTACGGTGTTGTACTATTGGAGCTAGTGACAGGCCGGCCAGCTGAGCAAGCGGAGCCATCCGAGTCTCTTGACATTGTAAAGTGGGTGCGACGGAAGGTCAACATTACAAATGGCGCTGTCCAAGTTCTCGATCCCAAAATAAAAAATTCTTCCCATCAAGAGATACTAGGAGCTTTAGAAATTGCTCTCTGTTGCACTTCTGTGATGCCGGAGAAAAGACCTTCGATGTCTGAAGTCGTGAGATCACTTCAGTCCCTGGACTTGAGGACCCACGGGGCAGTTATCGACTTCTCTTCCTTTGAGGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

885

Amino Acids

96.88

Weight (kDa)

6.33

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 6.5e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 116 - 224 1.6e-09 Leucine-rich repeat region
LRR_8 PF13855 146 - 203 4.2e-09 Leucine rich repeat
LRR_8 PF13855 434 - 493 9.2e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 605 - 864 1e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 605 - 862 2.8e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1701
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 2 cut(s) 200, 1090
AccB1I GGYRCC 1 cut(s) 161
AciI CCGC 2 cut(s) 1551, 2393
AclWI GGATC 4 cut(s) 116, 1885, 1898, 2468
AcoI YGGCCR 2 cut(s) 1932, 2378
AcsI RAATTY 5 cut(s) 571, 1134, 1278, 1882, 2488
AcuI CTGAAG 4 cut(s) 51, 1418, 2576, 2595
AcyI GRCGYC 1 cut(s) 1594
AfaI GTAC 4 cut(s) 406, 916, 2306, 2355
AfiI CCNNNNNNNGG 5 cut(s) 642, 1426, 1941, 2020, 2125
AflII CTTAAG 1 cut(s) 1030
AgeI ACCGGT 1 cut(s) 1412
AhdI GACNNNNNGTC 1 cut(s) 2438
AjnI CCWGG 5 cut(s) 143, 181, 513, 1555, 2598
AjuI GAANNNNNNNTTGG 4 cut(s) 1582, 1614, 2012, 2044
AleI CACNNNNGTG 2 cut(s) 902, 2543
Alw21I GWGCWC 3 cut(s) 306, 362, 1714
Alw26I GTCTC 3 cut(s) 365, 606, 2412
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 4 cut(s) 116, 1885, 1898, 2468
AlwNI CAGNNNCTG 1 cut(s) 2599
Ama87I CYCGRG 2 cut(s) 157, 1270
AoxI GGCC 7 cut(s) 108, 179, 1510, 1720, 1932, 2374, 2378
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 4 cut(s) 497, 1705, 1799, 2240
ApoI RAATTY 5 cut(s) 571, 1134, 1278, 1882, 2488
AsiGI ACCGGT 1 cut(s) 1412
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 4 cut(s) 1267, 1432, 2293, 2459
AspS9I GGNCC 4 cut(s) 147, 1415, 1553, 2610
AsuHPI GGTGA 9 cut(s) 91, 181, 217, 261, 272, 631, 1214, 1376, 1865
AsuII TTCGAA 1 cut(s) 1901
AvaI CYCGRG 2 cut(s) 157, 1270
AvaII GGWCC 4 cut(s) 147, 1415, 1553, 2610
AxyI CCTNAGG 1 cut(s) 1284
BaeGI GKGCMC 3 cut(s) 164, 362, 558
BaeI ACNNNNGTAYC 2 cut(s) 773, 806
BalI TGGCCA 1 cut(s) 1934
BamHI GGATCC 1 cut(s) 1890
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 3 cut(s) 288, 306, 1714
BbsI GAAGAC 2 cut(s) 1890, 1919
Bbv12I GWGCWC 3 cut(s) 306, 362, 1714
BbvCI CCTCAGC 1 cut(s) 887
BbvI GCAGC 4 cut(s) 484, 1717, 1786, 2227
BccI CCATC 4 cut(s) 344, 1771, 2407, 2505
BciT130I CCWGG 5 cut(s) 145, 183, 515, 1557, 2600
BciVI GTATCC 1 cut(s) 1358
BcoDI GTCTC 3 cut(s) 365, 606, 2412
BfaI CTAG 6 cut(s) 584, 785, 1128, 1307, 2366, 2510
BfmI CTRYAG 2 cut(s) 1800, 2044
BfoI RGCGCY 2 cut(s) 1433, 2460
BfrI CTTAAG 1 cut(s) 1030
BfuAI ACCTGC 2 cut(s) 200, 1090
BfuI GTATCC 1 cut(s) 1358
BisI GCNGC 4 cut(s) 498, 1706, 1800, 2241
BlpI GCTNAGC 1 cut(s) 2385
BlsI GCNGC 4 cut(s) 499, 1707, 1801, 2242
Bme1390I CCNGG 5 cut(s) 145, 183, 515, 1557, 2600
Bme18I GGWCC 4 cut(s) 147, 1415, 1553, 2610
BmeRI GACNNNNNGTC 1 cut(s) 2438
BmeT110I CYCGRG 2 cut(s) 157, 1270
BmgT120I GGNCC 4 cut(s) 147, 1415, 1553, 2610
BmiI GGNNCC 8 cut(s) 163, 287, 774, 1417, 1657, 1892, 2397, 2612
BmrFI CCNGG 5 cut(s) 145, 183, 515, 1557, 2600
BmrI ACTGGG 1 cut(s) 989
BmsI GCATC 3 cut(s) 867, 2173, 2538
BmuI ACTGGG 1 cut(s) 989
BpiI GAAGAC 2 cut(s) 1890, 1919
BpmI CTGGAG 2 cut(s) 389, 497
Bpu10I CCTNAGC 1 cut(s) 887
Bpu1102I GCTNAGC 1 cut(s) 2385
Bpu14I TTCGAA 1 cut(s) 1901
BpuEI CTTGAG 7 cut(s) 475, 680, 928, 971, 1698, 2144, 2626
Bsa29I ATCGAT 1 cut(s) 1693
BsaHI GRCGYC 1 cut(s) 1594
BsaJI CCNNGG 5 cut(s) 711, 1929, 2256, 2598, 2614
BsaWI WCCGGW 1 cut(s) 1412
Bsc4I CCNNNNNNNGG 5 cut(s) 642, 1426, 1941, 2020, 2125
Bse118I RCCGGY 2 cut(s) 1412, 2376
Bse1I ACTGG 5 cut(s) 769, 995, 1373, 1894, 2115
Bse21I CCTNAGG 1 cut(s) 1284
Bse3DI GCAATG 3 cut(s) 492, 1010, 2097
BseBI CCWGG 5 cut(s) 145, 183, 515, 1557, 2600
BseCI ATCGAT 1 cut(s) 1693
BseDI CCNNGG 5 cut(s) 711, 1929, 2256, 2598, 2614
BseGI GGATG 7 cut(s) 19, 336, 480, 1792, 2188, 2338, 2399
BseLI CCNNNNNNNGG 5 cut(s) 642, 1426, 1941, 2020, 2125
BseMI GCAATG 3 cut(s) 492, 1010, 2097
BseNI ACTGG 5 cut(s) 769, 995, 1373, 1894, 2115
BseSI GKGCMC 3 cut(s) 164, 362, 558
BseXI GCAGC 4 cut(s) 484, 1717, 1786, 2227
BseYI CCCAGC 1 cut(s) 1516
BsgI GTGCAG 2 cut(s) 178, 483
BshFI GGCC 7 cut(s) 110, 181, 1512, 1722, 1934, 2376, 2380
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 1412
BshVI ATCGAT 1 cut(s) 1693
BsiHKAI GWGCWC 3 cut(s) 306, 362, 1714
BsiHKCI CYCGRG 2 cut(s) 157, 1270
BsiSI CCGG 3 cut(s) 1413, 2377, 2552
BslFI GGGAC 4 cut(s) 1401, 1475, 2048, 2581
BslI CCNNNNNNNGG 5 cut(s) 642, 1426, 1941, 2020, 2125
BsmAI GTCTC 3 cut(s) 365, 606, 2412
BsmFI GGGAC 4 cut(s) 1401, 1475, 2048, 2581
BsmI GAATGC 2 cut(s) 944, 2303
BsnI GGCC 7 cut(s) 110, 181, 1512, 1722, 1934, 2376, 2380
BsoBI CYCGRG 2 cut(s) 157, 1270
Bsp119I TTCGAA 1 cut(s) 1901
Bsp1286I GDGCHC 6 cut(s) 164, 288, 306, 362, 558, 1714
Bsp143I GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
Bsp1720I GCTNAGC 1 cut(s) 2385
BspACI CCGC 2 cut(s) 1551, 2393
BspANI GGCC 7 cut(s) 110, 181, 1512, 1722, 1934, 2376, 2380
BspDI ATCGAT 1 cut(s) 1693
BspLI GGNNCC 8 cut(s) 163, 287, 774, 1417, 1657, 1892, 2397, 2612
BspMAI CTGCAG 2 cut(s) 1804, 2048
BspMI ACCTGC 2 cut(s) 200, 1090
BspPI GGATC 4 cut(s) 116, 1885, 1898, 2468
BspT104I TTCGAA 1 cut(s) 1901
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 1030
BsrDI GCAATG 3 cut(s) 492, 1010, 2097
BsrFI RCCGGY 2 cut(s) 1412, 2376
BsrI ACTGG 5 cut(s) 769, 995, 1373, 1894, 2115
BssAI RCCGGY 2 cut(s) 1412, 2376
BssECI CCNNGG 5 cut(s) 711, 1929, 2256, 2598, 2614
BssMI GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
BssNI GRCGYC 1 cut(s) 1594
BssT1I CCWWGG 3 cut(s) 711, 1929, 2256
Bst2UI CCWGG 5 cut(s) 145, 183, 515, 1557, 2600
Bst4CI ACNGT 3 cut(s) 467, 2309, 2348
Bst6I CTCTTC 2 cut(s) 1460, 2641
BstACI GRCGYC 1 cut(s) 1594
BstAFI CTTAAG 1 cut(s) 1030
BstBI TTCGAA 1 cut(s) 1901
BstC8I GCNNGC 6 cut(s) 1449, 1510, 1710, 2378, 2382, 2391
BstDSI CCRYGG 1 cut(s) 2614
BstENI CCTNNNNNAGG 2 cut(s) 1424, 2123
BstF5I GGATG 7 cut(s) 19, 336, 480, 1792, 2188, 2338, 2399
BstH2I RGCGCY 2 cut(s) 1433, 2460
BstHHI GCGC 4 cut(s) 1267, 1432, 2293, 2459
BstKTI GATC 9 cut(s) 124, 598, 1393, 1609, 1893, 1923, 2043, 2476, 2588
BstMAI GTCTC 3 cut(s) 365, 606, 2412
BstMBI GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
BstMWI GCNNNNNNNGC 6 cut(s) 497, 655, 1128, 2386, 2390, 2395
BstNI CCWGG 5 cut(s) 145, 183, 515, 1557, 2600
BstSCI CCNGG 5 cut(s) 143, 181, 513, 1555, 2598
BstSFI CTRYAG 2 cut(s) 1800, 2044
BstSLI GKGCMC 3 cut(s) 164, 362, 558
BstV1I GCAGC 4 cut(s) 484, 1717, 1786, 2227
BstV2I GAAGAC 2 cut(s) 1890, 1919
BstX2I RGATCY 2 cut(s) 121, 1890
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 121, 1890
Bsu15I ATCGAT 1 cut(s) 1693
Bsu36I CCTNAGG 1 cut(s) 1284
BsuI GTATCC 1 cut(s) 1358
BsuRI GGCC 7 cut(s) 110, 181, 1512, 1722, 1934, 2376, 2380
BsuTUI ATCGAT 1 cut(s) 1693
BtgI CCRYGG 1 cut(s) 2614
BtsCI GGATG 7 cut(s) 19, 336, 480, 1792, 2188, 2338, 2399
BtsI GCAGTG 2 cut(s) 195, 2072
BtsIMutI CAGTG 5 cut(s) 195, 362, 1380, 1758, 2072
BveI ACCTGC 2 cut(s) 200, 1090
Cac8I GCNNGC 6 cut(s) 1449, 1510, 1710, 2378, 2382, 2391
CaiI CAGNNNCTG 1 cut(s) 2599
CfoI GCGC 4 cut(s) 1267, 1432, 2293, 2459
Cfr10I RCCGGY 2 cut(s) 1412, 2376
Cfr13I GGNCC 4 cut(s) 147, 1415, 1553, 2610
ClaI ATCGAT 1 cut(s) 1693
CseI GACGC 1 cut(s) 1602
CsiI ACCWGGT 1 cut(s) 143
Csp6I GTAC 4 cut(s) 405, 915, 2305, 2354
CspAI ACCGGT 1 cut(s) 1412
CspCI CAANNNNNGTGG 2 cut(s) 1321, 1356
CviAII CATG 3 cut(s) 692, 1142, 1766
CviQI GTAC 4 cut(s) 405, 915, 2305, 2354
DpnI GATC 9 cut(s) 123, 597, 1392, 1608, 1892, 1922, 2042, 2475, 2587
DpnII GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
DriI GACNNNNNGTC 1 cut(s) 2438
EaeI YGGCCR 2 cut(s) 1932, 2378
Eam1104I CTCTTC 2 cut(s) 1460, 2641
Eam1105I GACNNNNNGTC 1 cut(s) 2438
EarI CTCTTC 2 cut(s) 1460, 2641
Ecl136II GAGCTC 2 cut(s) 304, 1712
Eco130I CCWWGG 3 cut(s) 711, 1929, 2256
Eco147I AGGCCT 2 cut(s) 110, 1512
Eco24I GRGCYC 3 cut(s) 288, 306, 1714
Eco32I GATATC 1 cut(s) 1635
Eco47I GGWCC 4 cut(s) 147, 1415, 1553, 2610
Eco53kI GAGCTC 2 cut(s) 304, 1712
Eco57I CTGAAG 4 cut(s) 51, 1418, 2576, 2595
Eco81I CCTNAGG 1 cut(s) 1284
Eco88I CYCGRG 2 cut(s) 157, 1270
EcoICRI GAGCTC 2 cut(s) 304, 1712
EcoNI CCTNNNNNAGG 2 cut(s) 1424, 2123
EcoO109I RGGNCCY 1 cut(s) 2610
EcoRII CCWGG 5 cut(s) 143, 181, 513, 1555, 2598
EcoRV GATATC 1 cut(s) 1635
EcoT14I CCWWGG 3 cut(s) 711, 1929, 2256
EcoT38I GRGCYC 3 cut(s) 288, 306, 1714
ErhI CCWWGG 3 cut(s) 711, 1929, 2256
FaeI CATG 3 cut(s) 695, 1145, 1769
FalI AAGNNNNNCTT 6 cut(s) 656, 688, 1707, 1739, 2012, 2044
FaqI GGGAC 4 cut(s) 1401, 1475, 2048, 2581
FatI CATG 3 cut(s) 691, 1141, 1765
Fnu4HI GCNGC 4 cut(s) 498, 1706, 1800, 2241
FokI GGATG 7 cut(s) 6, 323, 467, 1799, 2195, 2345, 2386
FriOI GRGCYC 3 cut(s) 288, 306, 1714
FseI GGCCGGCC 1 cut(s) 2380
Fsp4HI GCNGC 4 cut(s) 498, 1706, 1800, 2241
FspBI CTAG 6 cut(s) 584, 785, 1128, 1307, 2366, 2510
GlaI GCGC 4 cut(s) 1266, 1431, 2292, 2458
GluI GCNGC 4 cut(s) 498, 1706, 1800, 2241
GsaI CCCAGC 1 cut(s) 1520
GsuI CTGGAG 2 cut(s) 389, 497
HaeII RGCGCY 2 cut(s) 1433, 2460
HaeIII GGCC 7 cut(s) 110, 181, 1512, 1722, 1934, 2376, 2380
HapII CCGG 3 cut(s) 1413, 2377, 2552
HgaI GACGC 1 cut(s) 1602
HhaI GCGC 4 cut(s) 1267, 1432, 2293, 2459
Hin1I GRCGYC 1 cut(s) 1594
Hin1II CATG 3 cut(s) 695, 1145, 1769
Hin6I GCGC 4 cut(s) 1265, 1430, 2291, 2457
HinP1I GCGC 4 cut(s) 1265, 1430, 2291, 2457
HincII GTYRAC 1 cut(s) 2443
HindII GTYRAC 1 cut(s) 2443
HindIII AAGCTT 5 cut(s) 244, 647, 656, 1445, 1874
HpaII CCGG 3 cut(s) 1413, 2377, 2552
HphI GGTGA 9 cut(s) 91, 181, 217, 261, 272, 631, 1214, 1376, 1865
Hpy166II GTNNAC 8 cut(s) 360, 722, 1205, 1831, 1844, 1856, 2338, 2443
Hpy8I GTNNAC 8 cut(s) 360, 722, 1205, 1831, 1844, 1856, 2338, 2443
Hpy99I CGWCG 1 cut(s) 2436
HpyCH4III ACNGT 3 cut(s) 467, 2309, 2348
HpyF10VI GCNNNNNNNGC 6 cut(s) 497, 655, 1128, 2386, 2390, 2395
Hsp92I GRCGYC 1 cut(s) 1594
Hsp92II CATG 3 cut(s) 695, 1145, 1769
HspAI GCGC 4 cut(s) 1265, 1430, 2291, 2457
KroI GCCGGC 1 cut(s) 2376
KroNI GCCGGC 1 cut(s) 2378
Kzo9I GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
LmnI GCTCC 6 cut(s) 291, 1096, 2025, 2362, 2395, 2513
Lsp1109I GCAGC 4 cut(s) 484, 1717, 1786, 2227
LweI GCATC 3 cut(s) 867, 2173, 2538
MabI ACCWGGT 1 cut(s) 143
MaeI CTAG 6 cut(s) 584, 785, 1128, 1307, 2366, 2510
MaeIII GTNAC 8 cut(s) 223, 260, 434, 863, 1223, 1756, 2282, 2368
MalI GATC 9 cut(s) 123, 597, 1392, 1608, 1892, 1922, 2042, 2475, 2587
MboI GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
MflI RGATCY 2 cut(s) 121, 1890
MhlI GDGCHC 6 cut(s) 164, 288, 306, 362, 558, 1714
MlsI TGGCCA 1 cut(s) 1934
MluNI TGGCCA 1 cut(s) 1934
MlyI GAGTC 5 cut(s) 1220, 1497, 1562, 1764, 2414
MmeI TCCRAC 4 cut(s) 174, 1074, 1633, 2038
Mox20I TGGCCA 1 cut(s) 1934
MroNI GCCGGC 1 cut(s) 2376
MroXI GAANNNNTTC 1 cut(s) 668
MscI TGGCCA 1 cut(s) 1934
MseI TTAA 8 cut(s) 117, 395, 761, 1023, 1031, 1188, 1290, 1470
MslI CAYNNNNRTG 3 cut(s) 900, 902, 2543
Msp20I TGGCCA 1 cut(s) 1934
MspA1I CMGCKG 1 cut(s) 2384
MspCI CTTAAG 1 cut(s) 1030
MspI CCGG 3 cut(s) 1413, 2377, 2552
MspR9I CCNGG 5 cut(s) 145, 183, 515, 1557, 2600
Mva1269I GAATGC 2 cut(s) 944, 2303
MvaI CCWGG 5 cut(s) 145, 183, 515, 1557, 2600
MwoI GCNNNNNNNGC 6 cut(s) 497, 655, 1128, 2386, 2390, 2395
NaeI GCCGGC 1 cut(s) 2378
NdeII GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
NgoMIV GCCGGC 1 cut(s) 2376
NlaIII CATG 3 cut(s) 695, 1145, 1769
NlaIV GGNNCC 8 cut(s) 163, 287, 774, 1417, 1657, 1892, 2397, 2612
NmuCI GTSAC 5 cut(s) 223, 260, 1223, 1756, 2368
NspV TTCGAA 1 cut(s) 1901
OliI CACNNNNGTG 2 cut(s) 902, 2543
PaqCI CACCTGC 1 cut(s) 200
PceI AGGCCT 2 cut(s) 110, 1512
PctI GAATGC 2 cut(s) 944, 2303
PdiI GCCGGC 1 cut(s) 2378
PdmI GAANNNNTTC 1 cut(s) 668
PfoI TCCNGGA 1 cut(s) 1555
PinAI ACCGGT 1 cut(s) 1412
PkrI GCNGC 4 cut(s) 499, 1707, 1801, 2242
PleI GAGTC 5 cut(s) 1220, 1496, 1562, 1763, 2413
PpsI GAGTC 5 cut(s) 1220, 1496, 1562, 1763, 2413
PpuMI RGGWCCY 1 cut(s) 2610
PsiI TTATAA 1 cut(s) 1701
Psp124BI GAGCTC 2 cut(s) 306, 1714
Psp5II RGGWCCY 1 cut(s) 2610
Psp6I CCWGG 5 cut(s) 143, 181, 513, 1555, 2598
PspFI CCCAGC 1 cut(s) 1516
PspGI CCWGG 5 cut(s) 143, 181, 513, 1555, 2598
PspN4I GGNNCC 8 cut(s) 163, 287, 774, 1417, 1657, 1892, 2397, 2612
PspPI GGNCC 4 cut(s) 147, 1415, 1553, 2610
PspPPI RGGWCCY 1 cut(s) 2610
PstI CTGCAG 2 cut(s) 1804, 2048
PstNI CAGNNNCTG 1 cut(s) 2599
PsuI RGATCY 2 cut(s) 121, 1890
PvuII CAGCTG 1 cut(s) 2384
RigI GGCCGGCC 1 cut(s) 2380
RsaI GTAC 4 cut(s) 406, 916, 2306, 2355
RsaNI GTAC 4 cut(s) 405, 915, 2305, 2354
RseI CAYNNNNRTG 3 cut(s) 900, 902, 2543
SacI GAGCTC 2 cut(s) 306, 1714
SaqAI TTAA 8 cut(s) 117, 395, 761, 1023, 1031, 1188, 1290, 1470
SatI GCNGC 4 cut(s) 498, 1706, 1800, 2241
Sau3AI GATC 9 cut(s) 121, 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
Sau96I GGNCC 4 cut(s) 147, 1415, 1553, 2610
SchI GAGTC 5 cut(s) 1220, 1497, 1562, 1764, 2414
ScrFI CCNGG 5 cut(s) 145, 183, 515, 1557, 2600
SduI GDGCHC 6 cut(s) 164, 288, 306, 362, 558, 1714
SexAI ACCWGGT 1 cut(s) 143
SfaNI GCATC 3 cut(s) 867, 2173, 2538
SfcI CTRYAG 2 cut(s) 1800, 2044
SfuI TTCGAA 1 cut(s) 1901
SinI GGWCC 4 cut(s) 147, 1415, 1553, 2610
SmiMI CAYNNNNRTG 3 cut(s) 900, 902, 2543
SmlI CTYRAG 8 cut(s) 454, 659, 943, 950, 1030, 1713, 2159, 2605
SmoI CTYRAG 8 cut(s) 454, 659, 943, 950, 1030, 1713, 2159, 2605
SseBI AGGCCT 2 cut(s) 110, 1512
SsiI CCGC 2 cut(s) 1551, 2393
SspI AATATT 1 cut(s) 1995
SspMI CTAG 6 cut(s) 584, 785, 1128, 1307, 2366, 2510
SstI GAGCTC 2 cut(s) 306, 1714
StuI AGGCCT 2 cut(s) 110, 1512
StyD4I CCNGG 5 cut(s) 143, 181, 513, 1555, 2598
StyI CCWWGG 3 cut(s) 711, 1929, 2256
TaaI ACNGT 3 cut(s) 467, 2309, 2348
TaqI TCGA 8 cut(s) 450, 1566, 1693, 1901, 2220, 2472, 2567, 2628
TatI WGTACW 2 cut(s) 2304, 2353
Tru1I TTAA 8 cut(s) 117, 395, 761, 1023, 1031, 1188, 1290, 1470
Tru9I TTAA 8 cut(s) 117, 395, 761, 1023, 1031, 1188, 1290, 1470
TscAI CASTG 5 cut(s) 202, 362, 1380, 1765, 2072
TseFI GTSAC 5 cut(s) 223, 260, 1223, 1756, 2368
TseI GCWGC 4 cut(s) 497, 1705, 1799, 2240
Tsp45I GTSAC 5 cut(s) 223, 260, 1223, 1756, 2368
TspDTI ATGAA 3 cut(s) 680, 1512, 2138
TspGWI ACGGA 3 cut(s) 211, 882, 2449
TspRI CASTG 5 cut(s) 202, 362, 1380, 1765, 2072
Vha464I CTTAAG 1 cut(s) 1030
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 4 cut(s) 147, 1415, 1553, 2610
XagI CCTNNNNNAGG 2 cut(s) 1424, 2123
XapI RAATTY 5 cut(s) 571, 1134, 1278, 1882, 2488
XcmI CCANNNNNNNNNTGG 1 cut(s) 1002
XmnI GAANNNNTTC 1 cut(s) 668
XspI CTAG 6 cut(s) 584, 785, 1128, 1307, 2366, 2510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.