pycom14g07820

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011484
Physical Location & Seq
Forward (+)
8115203 .. 8117663
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGGTACAATTTCATTCATCCGCTCGGCTCTCTGTGTGCGGTGTCTGTTTTGGGTGACGAGTGACATTGCAGTCCCAACAATGGCTGAGAAGGGTAAGGAGAAAGAATCGGATCCATTTCTGTACGAGAGTGAAGACGAAGAAGATGACATTGAGGAGAACAAGAGCGAGAGTGGGAGCAGCTCCACCAGCAGCCATGGCGTCCGTCATGAGGTTTCCTCTCCTGTTAGTTTTTCTTCTCACAAATGGCCTCAGAGTTACAGGGAGACAATCGATTCTTACTCAATTGCAGCATCACCCAATATTGCAAATCTCGGATTTGTTCCAAGTAATGCAAGCTTTACAAATTACTCGAAAAGCATTTTAGACCTGATTGGAAAGTCTCCATTCGTGTCTACCAACGAAGGTTTTCCTCAAAAGGGTGATTCGGAAAGGTTCTCAGTAAACCAATCATTATTATCAGAGAGATCGACACTGCATAAGCATCTGACCGGAGAAATACCTGTTGGTCATGGGTGTAGCTTCACTCAAACTGTCTTCAATGGAATTAATGTAATGGCAGGAGTTGGGCTTCTTTCAACACCTTCTACAATGAAAGAAGCTGGCTGGGCAGGTATCGTGGTGCTACTTCTTTTTGCAGTTGTGTGTTGTTACACGGCCATACTCATGAGATACTGCTTTGAGAGTAAAGAAGGCATCAAAACTTACCCGGATTTAGGAGAAGCTGCCTTTGGAAGATATGGGCGTCTTTTCATATCTCGATCATTTTGTACGCAGAACTATACTCTTATTGTGTAG

Protein Analysis

266

Amino Acids

29.08

Weight (kDa)

5.42

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 172 - 260 2.6e-17 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41190 AT2G41190
fragaria_vesca FvH4_6g25440
malus_domestica MD14G1088200.v1.1
prunus_persica Prupe.7G038500_v2.0.a1
pyrus_communis pycom14g07820 pycom14g07830
rosa_chinensis RchiOBHm_Chr2g0125581 RchiOBHm_Chr2g0125591
rosa_laevigata RLG00000018871
rosa_multiflora Rmu_sc0002643.1_g000007 Rmu_sc0014914.1_g000001
rosa_roxburghii Rroxscaffold_2G00117920
rosa_rugosa Rorug02G0258100
rosa_samantha Rh2AG316700 Rh2BG324400 Rh2BG324500 Rh2CG306500 Rh2DG342800
rosa_wichuraiana Rw2G025560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 602
AccBSI CCGCTC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 395
AciI CCGC 2 cut(s) 22, 40
AclWI GGATC 2 cut(s) 107, 120
AcoI YGGCCR 1 cut(s) 657
AcyI GRCGYC 2 cut(s) 201, 745
AfaI GTAC 3 cut(s) 7, 125, 772
AfiI CCNNNNNNNGG 4 cut(s) 82, 211, 419, 716
AgsI TTSAA 2 cut(s) 541, 579
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 714, 746
AluBI AGCT 5 cut(s) 183, 339, 522, 602, 725
AluI AGCT 5 cut(s) 183, 339, 522, 602, 725
Alw26I GTCTC 2 cut(s) 260, 387
AlwI GGATC 2 cut(s) 107, 120
AoxI GGCC 2 cut(s) 248, 657
ApeKI GCWGC 4 cut(s) 180, 192, 290, 725
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 408
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 3 cut(s) 67, 288, 434
BamHI GGATCC 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 141, 529
BbvI GCAGC 4 cut(s) 192, 204, 302, 712
BceAI ACGGC 1 cut(s) 672
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 2 cut(s) 260, 387
BfuAI ACCTGC 1 cut(s) 602
BisI GCNGC 4 cut(s) 181, 193, 291, 726
BlsI GCNGC 4 cut(s) 182, 194, 292, 727
Bme1390I CCNGG 1 cut(s) 710
BmiI GGNNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 710
BmsI GCATC 3 cut(s) 302, 493, 705
BpiI GAAGAC 2 cut(s) 141, 529
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpuMI CCSGG 1 cut(s) 710
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 201, 745
BsaJI CCNNGG 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 211, 419, 716
Bse3DI GCAATG 1 cut(s) 66
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 4 cut(s) 82, 211, 419, 716
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 3 cut(s) 78, 266, 453
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 192, 204, 302, 712
BseYI CCCAGC 1 cut(s) 606
BshFI GGCC 2 cut(s) 250, 659
BshVI ATCGAT 1 cut(s) 273
BsiSI CCGG 2 cut(s) 492, 710
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 4 cut(s) 82, 211, 419, 716
BsmAI GTCTC 2 cut(s) 260, 387
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 2 cut(s) 250, 659
Bsp143I GATC 3 cut(s) 112, 467, 761
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 2 cut(s) 22, 40
BspANI GGCC 2 cut(s) 250, 659
BspCNI CTCAG 3 cut(s) 79, 265, 452
BspDI ATCGAT 1 cut(s) 273
BspHI TCATGA 2 cut(s) 208, 666
BspLI GGNNCC 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 602
BspPI GGATC 2 cut(s) 107, 120
BsrBI CCGCTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 3 cut(s) 112, 467, 761
BssNI GRCGYC 2 cut(s) 201, 745
BssT1I CCWWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 535
BstACI GRCGYC 2 cut(s) 201, 745
BstC8I GCNNGC 2 cut(s) 337, 604
BstDEI CTNAG 3 cut(s) 87, 252, 439
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 18
BstKTI GATC 3 cut(s) 115, 470, 764
BstMAI GTCTC 2 cut(s) 260, 387
BstMBI GATC 3 cut(s) 112, 467, 761
BstMWI GCNNNNNNNGC 3 cut(s) 189, 198, 608
BstSCI CCNGG 1 cut(s) 708
BstV1I GCAGC 4 cut(s) 192, 204, 302, 712
BstV2I GAAGAC 2 cut(s) 141, 529
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 250, 659
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 18
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
BveI ACCTGC 1 cut(s) 602
Cac8I GCNNGC 2 cut(s) 337, 604
CciI TCATGA 2 cut(s) 208, 666
ClaI ATCGAT 1 cut(s) 273
CseI GACGC 2 cut(s) 190, 734
Csp6I GTAC 3 cut(s) 6, 124, 771
CviAII CATG 4 cut(s) 197, 209, 512, 667
CviQI GTAC 3 cut(s) 6, 124, 771
DdeI CTNAG 3 cut(s) 87, 252, 439
DpnI GATC 3 cut(s) 114, 469, 763
DpnII GATC 3 cut(s) 112, 467, 761
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 4 cut(s) 200, 212, 515, 670
FaiI YATR 9 cut(s) 198, 210, 480, 513, 662, 668, 741, 755, 783
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 196, 208, 511, 666
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 4 cut(s) 181, 193, 291, 726
FokI GGATG 1 cut(s) 5
Fsp4HI GCNGC 4 cut(s) 181, 193, 291, 726
GluI GCNGC 4 cut(s) 181, 193, 291, 726
GsaI CCCAGC 1 cut(s) 610
HaeIII GGCC 2 cut(s) 250, 659
HapII CCGG 2 cut(s) 492, 710
HgaI GACGC 2 cut(s) 190, 734
Hin1I GRCGYC 2 cut(s) 201, 745
Hin1II CATG 4 cut(s) 200, 212, 515, 670
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 3 cut(s) 107, 275, 425
HpaII CCGG 2 cut(s) 492, 710
HphI GGTGA 3 cut(s) 67, 288, 434
Hpy166II GTNNAC 2 cut(s) 396, 445
Hpy188I TCNGA 6 cut(s) 112, 255, 317, 430, 463, 489
Hpy188III TCNNGA 3 cut(s) 209, 667, 759
Hpy8I GTNNAC 2 cut(s) 396, 445
HpyAV CCTTC 4 cut(s) 85, 398, 594, 686
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4V TGCA 6 cut(s) 71, 290, 308, 335, 478, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 198, 608
HpyF3I CTNAG 3 cut(s) 87, 252, 439
Hsp92I GRCGYC 2 cut(s) 201, 745
Hsp92II CATG 4 cut(s) 200, 212, 515, 670
Kzo9I GATC 3 cut(s) 112, 467, 761
LmnI GCTCC 2 cut(s) 177, 188
Lsp1109I GCAGC 4 cut(s) 192, 204, 302, 712
LweI GCATC 3 cut(s) 302, 493, 705
MaeIII GTNAC 4 cut(s) 55, 62, 257, 650
MalI GATC 3 cut(s) 114, 469, 763
MbiI CCGCTC 1 cut(s) 24
MboI GATC 3 cut(s) 112, 467, 761
MboII GAAGA 6 cut(s) 146, 152, 155, 228, 529, 747
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 112
MluCI AATT 4 cut(s) 9, 285, 346, 546
MnlI CCTC 5 cut(s) 148, 205, 229, 261, 423
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 665
MspI CCGG 2 cut(s) 492, 710
MspR9I CCNGG 1 cut(s) 710
MunI CAATTG 1 cut(s) 285
MwoI GCNNNNNNNGC 3 cut(s) 189, 198, 608
NciI CCSGG 1 cut(s) 710
NcoI CCATGG 1 cut(s) 196
NdeII GATC 3 cut(s) 112, 467, 761
NlaIII CATG 4 cut(s) 200, 212, 515, 670
NlaIV GGNNCC 1 cut(s) 114
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 2 cut(s) 55, 62
PagI TCATGA 2 cut(s) 208, 666
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 107, 275, 425
PkrI GCNGC 4 cut(s) 182, 194, 292, 727
PshBI ATTAAT 1 cut(s) 549
PspFI CCCAGC 1 cut(s) 606
PspN4I GGNNCC 1 cut(s) 114
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 3 cut(s) 7, 125, 772
RsaNI GTAC 3 cut(s) 6, 124, 771
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 4 cut(s) 181, 193, 291, 726
Sau3AI GATC 3 cut(s) 112, 467, 761
ScrFI CCNGG 1 cut(s) 710
SfaNI GCATC 3 cut(s) 302, 493, 705
SmiMI CAYNNNNRTG 1 cut(s) 665
Sse9I AATT 4 cut(s) 9, 285, 346, 546
SsiI CCGC 2 cut(s) 22, 40
SspI AATATT 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 708
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 535
TaqI TCGA 4 cut(s) 273, 353, 470, 760
TasI AATT 4 cut(s) 9, 285, 346, 546
TfiI GAWTC 3 cut(s) 107, 275, 425
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 2 cut(s) 55, 62
TseI GCWGC 4 cut(s) 180, 192, 290, 725
Tsp45I GTSAC 2 cut(s) 55, 62
TspDTI ATGAA 3 cut(s) 7, 608, 742
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 1 cut(s) 480
VspI ATTAAT 1 cut(s) 549
XmiI GTMKAC 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.