pycom14g10610

Monodehydroascorbate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005394
Physical Location & Seq
Forward (+)
13356280 .. 13360409
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGTCTTCAGTTCGCAGGCGAATGGCGAACCTGGCCAATTCCGTGTGCTTCAAGCACTCTGTTGCTCTCCGGCCTGATCGCCGTTCTTCTCCAATCGGATTCAGGAGCTTCCGCCGAAGCTACGTTGTTTCGTCCTCAGATTTCGCCAACGAAAACCGAGAGTTTGTGATTGTTGGTGGCGGCAATGCGGCTGGCTATGCAGCTAGGACTTTCGTCGAACACGGAATGGCCGATGGACGCCTTGCAATCGTGTCCAAAGAGGCCGTTGCGCCTTATGAGCGTCCTGCTTTGACAAAAGGCTATTTGTTCCCTTCGGATAAGAAGCCAGCACGATTGCCTGGGTTTCATACTTGTGTTGGATCCGGCGGTGATAGGCAAACACCTGACTGGTATAAAGAAAAAGGGATAGAGATGTTCTATGAAGATCCCGTTACAAATGTTGATATTGAAAAGCAGACACTGACTACAAATTCAGGCAAACTGCTCAAGTTTGGATCACTTATAATTGCAACAGGATGTATAGCCTCAAGATTTCCTGAGAAAATTGGAGGAAACCTACCTGGTGTTCATTATATTCGGGATGTTGCAGATGCTGATGCACTAGTATCATCATTGGAGAAAGCACGGAAGGTGGTTGTTGTTGGTGGTGGTTATATTGGTATGGAGGTTGCTGCAGCCGCTATTGGTTGGAAACTTGATACAACAATCATATTTCCAGAAAACCATCTGTTGCAAAGATTGTTTACTGCTTCACTTGCCCGGAGATATGAGGAATTCTACCAAGAGAATGGTGTTAAATTCATAAAGGGTGCCTCAATTAAATATTTAGAAGCTGGTGCTGATGGACGTGTAAATTATGTTAAGCTTGAAGATGGAACTACTATAGAAGCAGACACAGTAGTTATTGGTATTGGAGCAAAACCTGCTGTTGGTCCTTTTGAAAGTGTCGGTTTGGATTCCAATGTTGGTGGTATACAGGTTGATGGTCTGTTCCGGACGAGGATACCTGGAATCTTCGCAGTTGGAGATGTTGCAGCATTTCCATTGAAGATGTATAACCGTATAGCACGAGTTGAACATGTAGATCATGCTCGTCGATCTGCACAGCATTGCGTTAAGGCTTTAGTGAGTGCACAAACTGATACGTATGATTACCTCCCATCTTTTTACTCGAGGGTGTTTGAATATGAAGGAAGCCCCAGGAAAATATGGTGGCAATTTTTTGGGGATAACGTTGGAGAGACTATTGAAATTGGGAATTTTAATCCTAAGATTGCCACTTTCTGGATAGATTCTGGCAAATTGAAAGGTGTTCTTCTTGAGAGCGGAAATCCCGAGGAATTTCAACTCCTTCCAAAACTTGCAAGGAGCCAGCCCATTGTCGATAAAGCCAAGCTTCAGAATGCATCTTCGGTTGAGGAGGCACTGGACATTGTTCAGTCATCGTTATAG

Protein Analysis

484

Amino Acids

53.0

Weight (kDa)

8.63

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 55 - 367 9e-55 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 210 - 292 1.5e-15 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 386 - 466 2.8e-17 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 931
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 1284
AccBSI CCGCTC 1 cut(s) 1326
AccI GTMKAC 1 cut(s) 973
AccIII TCCGGA 1 cut(s) 993
AciI CCGC 6 cut(s) 112, 180, 188, 366, 678, 1326
AclI AACGTT 1 cut(s) 1233
AclWI GGATC 4 cut(s) 354, 367, 419, 502
AcoI YGGCCR 2 cut(s) 33, 228
AcsI RAATTY 5 cut(s) 469, 773, 797, 1258, 1340
AcuI CTGAAG 1 cut(s) 1382
AcyI GRCGYC 1 cut(s) 238
AfiI CCNNNNNNNGG 2 cut(s) 929, 1284
AflIII ACRYGT 2 cut(s) 847, 1078
AhlI ACTAGT 1 cut(s) 601
AjiI CACGTC 1 cut(s) 848
AjnI CCWGG 5 cut(s) 30, 337, 559, 1006, 1199
AluBI AGCT 6 cut(s) 108, 120, 203, 833, 865, 1396
AluI AGCT 6 cut(s) 108, 120, 203, 833, 865, 1396
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 1 cut(s) 1235
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 4 cut(s) 354, 367, 419, 502
AlwNI CAGNNNCTG 2 cut(s) 460, 593
Ama87I CYCGRG 2 cut(s) 1171, 1334
Aor13HI TCCGGA 1 cut(s) 993
AoxI GGCC 4 cut(s) 33, 71, 228, 261
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 4 cut(s) 200, 671, 674, 1034
ApoI RAATTY 5 cut(s) 469, 773, 797, 1258, 1340
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 1 cut(s) 932
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 1 cut(s) 380
AvaI CYCGRG 2 cut(s) 1171, 1334
AvaII GGWCC 1 cut(s) 932
BaeGI GKGCMC 1 cut(s) 1135
BalI TGGCCA 1 cut(s) 35
BamHI GGATCC 1 cut(s) 359
BanI GGYRCC 1 cut(s) 809
BauI CACGAG 1 cut(s) 1068
Bbv12I GWGCWC 1 cut(s) 1135
BbvI GCAGC 4 cut(s) 212, 658, 686, 1046
BccI CCATC 6 cut(s) 227, 732, 836, 866, 977, 1168
BceAI ACGGC 2 cut(s) 66, 248
BciT130I CCWGG 5 cut(s) 32, 339, 561, 1008, 1201
BciVI GTATCC 1 cut(s) 996
BcnI CCSGG 1 cut(s) 760
BcoDI GTCTC 1 cut(s) 1235
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 2 cut(s) 204, 602
BfmI CTRYAG 2 cut(s) 672, 882
BfuAI ACCTGC 1 cut(s) 931
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 7 cut(s) 181, 189, 201, 672, 675, 678, 1035
BlsI GCNGC 7 cut(s) 182, 190, 202, 673, 676, 679, 1036
Bme1390I CCNGG 6 cut(s) 32, 339, 561, 760, 1008, 1201
Bme18I GGWCC 1 cut(s) 932
BmeT110I CYCGRG 2 cut(s) 1171, 1334
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 932
BmiI GGNNCC 3 cut(s) 361, 811, 1370
BmrFI CCNGG 6 cut(s) 32, 339, 561, 760, 1008, 1201
BmsI GCATC 3 cut(s) 580, 586, 1415
BoxI GACNNNNGTC 1 cut(s) 212
BpuEI CTTGAG 3 cut(s) 470, 511, 1340
BpuMI CCSGG 1 cut(s) 760
BsaAI YACGTR 1 cut(s) 1146
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 3 cut(s) 338, 1199, 1335
BsaWI WCCGGW 1 cut(s) 993
Bsc4I CCNNNNNNNGG 2 cut(s) 929, 1284
Bse1I ACTGG 2 cut(s) 392, 1431
Bse3DI GCAATG 2 cut(s) 190, 1108
BseAI TCCGGA 1 cut(s) 993
BseBI CCWGG 5 cut(s) 32, 339, 561, 1008, 1201
BseDI CCNNGG 3 cut(s) 338, 1199, 1335
BseGI GGATG 2 cut(s) 521, 586
BseLI CCNNNNNNNGG 2 cut(s) 929, 1284
BseMI GCAATG 2 cut(s) 190, 1108
BseMII CTCAG 2 cut(s) 150, 528
BseNI ACTGG 2 cut(s) 392, 1431
BseRI GAGGAG 1 cut(s) 1433
BseSI GKGCMC 1 cut(s) 1135
BseXI GCAGC 4 cut(s) 212, 658, 686, 1046
BsgI GTGCAG 1 cut(s) 1086
BshFI GGCC 4 cut(s) 35, 73, 230, 263
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 1135
BsiHKCI CYCGRG 2 cut(s) 1171, 1334
BsiSI CCGG 4 cut(s) 70, 363, 760, 994
BslI CCNNNNNNNGG 2 cut(s) 929, 1284
BsmAI GTCTC 1 cut(s) 1235
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 4 cut(s) 35, 73, 230, 263
BsoBI CYCGRG 2 cut(s) 1171, 1334
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp13I TCCGGA 1 cut(s) 993
Bsp143I GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
BspACI CCGC 6 cut(s) 112, 180, 188, 366, 678, 1326
BspANI GGCC 4 cut(s) 35, 73, 230, 263
BspCNI CTCAG 2 cut(s) 149, 529
BspEI TCCGGA 1 cut(s) 993
BspLI GGNNCC 3 cut(s) 361, 811, 1370
BspMAI CTGCAG 1 cut(s) 676
BspMI ACCTGC 1 cut(s) 931
BspPI GGATC 4 cut(s) 354, 367, 419, 502
BspT107I GGYRCC 1 cut(s) 809
BsrBI CCGCTC 1 cut(s) 1326
BsrDI GCAATG 2 cut(s) 190, 1108
BsrI ACTGG 2 cut(s) 392, 1431
BssECI CCNNGG 3 cut(s) 338, 1199, 1335
BssMI GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
BssNAI GTATAC 1 cut(s) 974
BssNI GRCGYC 1 cut(s) 238
BssSI CACGAG 1 cut(s) 1068
Bst1107I GTATAC 1 cut(s) 974
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 5 cut(s) 32, 339, 561, 1008, 1201
Bst4CI ACNGT 2 cut(s) 898, 1061
BstACI GRCGYC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 923
BstBAI YACGTR 1 cut(s) 1146
BstC8I GCNNGC 4 cut(s) 17, 193, 327, 1373
BstDEI CTNAG 3 cut(s) 136, 537, 1269
BstF5I GGATG 2 cut(s) 521, 586
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 6 cut(s) 79, 362, 427, 497, 1087, 1100
BstMAI GTCTC 1 cut(s) 1235
BstMBI GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
BstMWI GCNNNNNNNGC 6 cut(s) 32, 197, 277, 677, 755, 923
BstNI CCWGG 5 cut(s) 32, 339, 561, 1008, 1201
BstNSI RCATGY 1 cut(s) 1082
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 6 cut(s) 30, 337, 559, 758, 1006, 1199
BstSFI CTRYAG 2 cut(s) 672, 882
BstSLI GKGCMC 1 cut(s) 1135
BstSNI TACGTA 1 cut(s) 1146
BstV1I GCAGC 4 cut(s) 212, 658, 686, 1046
BstX2I RGATCY 2 cut(s) 359, 424
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 2 cut(s) 359, 424
BstZ17I GTATAC 1 cut(s) 974
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 4 cut(s) 35, 73, 230, 263
BtrI CACGTC 1 cut(s) 848
BtsCI GGATG 2 cut(s) 521, 586
BtsIMutI CAGTG 2 cut(s) 458, 1424
BveI ACCTGC 1 cut(s) 931
Cac8I GCNNGC 4 cut(s) 17, 193, 327, 1373
CaiI CAGNNNCTG 2 cut(s) 460, 593
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 932
CseI GACGC 2 cut(s) 246, 269
CsiI ACCWGGT 1 cut(s) 559
CspCI CAANNNNNGTGG 2 cut(s) 949, 984
CviAII CATG 2 cut(s) 1079, 1088
DdeI CTNAG 3 cut(s) 136, 537, 1269
DpnI GATC 6 cut(s) 78, 361, 426, 496, 1086, 1099
DpnII GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
EaeI YGGCCR 2 cut(s) 33, 228
EciI GGCGGA 1 cut(s) 101
Eco105I TACGTA 1 cut(s) 1146
Eco47I GGWCC 1 cut(s) 932
Eco57I CTGAAG 1 cut(s) 1382
Eco88I CYCGRG 2 cut(s) 1171, 1334
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 5 cut(s) 30, 337, 559, 1006, 1199
EcoT22I ATGCAT 1 cut(s) 1408
FaeI CATG 2 cut(s) 1082, 1091
FalI AAGNNNNNCTT 4 cut(s) 1299, 1331, 1380, 1412
FatI CATG 2 cut(s) 1078, 1087
FblI GTMKAC 1 cut(s) 973
Fnu4HI GCNGC 7 cut(s) 181, 189, 201, 672, 675, 678, 1035
FokI GGATG 2 cut(s) 528, 593
Fsp4HI GCNGC 7 cut(s) 181, 189, 201, 672, 675, 678, 1035
FspBI CTAG 2 cut(s) 204, 602
GlaI GCGC 1 cut(s) 270
GluI GCNGC 7 cut(s) 181, 189, 201, 672, 675, 678, 1035
HaeIII GGCC 4 cut(s) 35, 73, 230, 263
HapII CCGG 4 cut(s) 70, 363, 760, 994
HgaI GACGC 2 cut(s) 246, 269
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 2 cut(s) 1082, 1091
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HindIII AAGCTT 2 cut(s) 863, 1394
HinfI GANTC 4 cut(s) 99, 956, 1011, 1292
HpaII CCGG 4 cut(s) 70, 363, 760, 994
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 3 cut(s) 744, 974, 1133
Hpy188I TCNGA 4 cut(s) 98, 139, 316, 1401
Hpy188III TCNNGA 9 cut(s) 103, 528, 536, 578, 716, 994, 1285, 1319, 1334
Hpy8I GTNNAC 3 cut(s) 744, 974, 1133
Hpy99I CGWCG 2 cut(s) 218, 1098
HpyAV CCTTC 4 cut(s) 321, 622, 1184, 1361
HpyCH4III ACNGT 2 cut(s) 898, 1061
HpyCH4IV ACGT 4 cut(s) 123, 847, 1145, 1233
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 197, 277, 677, 755, 923
HpyF3I CTNAG 3 cut(s) 136, 537, 1269
HpySE526I ACGT 4 cut(s) 123, 847, 1145, 1233
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 2 cut(s) 1082, 1091
HspAI GCGC 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 993
Kzo9I GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
LmnI GCTCC 3 cut(s) 105, 914, 1368
Lsp1109I GCAGC 4 cut(s) 212, 658, 686, 1046
LweI GCATC 3 cut(s) 580, 586, 1415
MabI ACCWGGT 1 cut(s) 559
MaeI CTAG 2 cut(s) 204, 602
MaeII ACGT 4 cut(s) 123, 847, 1145, 1233
MaeIII GTNAC 1 cut(s) 430
MalI GATC 6 cut(s) 78, 361, 426, 496, 1086, 1099
MbiI CCGCTC 1 cut(s) 1326
MboI GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
MboII GAAGA 7 cut(s) 78, 434, 881, 1006, 1060, 1307, 1401
MflI RGATCY 2 cut(s) 359, 424
MhlI GDGCHC 1 cut(s) 1135
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MmeI TCCRAC 4 cut(s) 337, 668, 1003, 1216
Mox20I TGGCCA 1 cut(s) 35
Mph1103I ATGCAT 1 cut(s) 1408
MroI TCCGGA 1 cut(s) 993
MscI TGGCCA 1 cut(s) 35
MseI TTAA 5 cut(s) 795, 819, 861, 1116, 1263
MslI CAYNNNNRTG 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 4 cut(s) 70, 363, 760, 994
MspR9I CCNGG 6 cut(s) 32, 339, 561, 760, 1008, 1201
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 5 cut(s) 32, 339, 561, 1008, 1201
MwoI GCNNNNNNNGC 6 cut(s) 32, 197, 277, 677, 755, 923
NciI CCSGG 1 cut(s) 760
NdeII GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
NlaIII CATG 2 cut(s) 1082, 1091
NlaIV GGNNCC 3 cut(s) 361, 811, 1370
NsiI ATGCAT 1 cut(s) 1408
NspI RCATGY 1 cut(s) 1082
PaeR7I CTCGAG 1 cut(s) 1171
PciI ACATGT 1 cut(s) 1078
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 1408
PfeI GAWTC 4 cut(s) 99, 956, 1011, 1292
PflMI CCANNNNNTGG 1 cut(s) 1284
PkrI GCNGC 7 cut(s) 182, 190, 202, 673, 676, 679, 1036
Ppu21I YACGTR 1 cut(s) 1146
PscI ACATGT 1 cut(s) 1078
PshAI GACNNNNGTC 1 cut(s) 212
PsiI TTATAA 1 cut(s) 503
Psp1406I AACGTT 1 cut(s) 1233
Psp6I CCWGG 5 cut(s) 30, 337, 559, 1006, 1199
PspGI CCWGG 5 cut(s) 30, 337, 559, 1006, 1199
PspN4I GGNNCC 3 cut(s) 361, 811, 1370
PspPI GGNCC 1 cut(s) 932
PspXI VCTCGAGB 1 cut(s) 1171
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 2 cut(s) 460, 593
PsuI RGATCY 2 cut(s) 359, 424
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 5 cut(s) 795, 819, 861, 1116, 1263
SatI GCNGC 7 cut(s) 181, 189, 201, 672, 675, 678, 1035
Sau3AI GATC 6 cut(s) 76, 359, 424, 494, 1084, 1097
Sau96I GGNCC 1 cut(s) 932
ScrFI CCNGG 6 cut(s) 32, 339, 561, 760, 1008, 1201
SduI GDGCHC 1 cut(s) 1135
SexAI ACCWGGT 1 cut(s) 559
SfaNI GCATC 3 cut(s) 580, 586, 1415
SfcI CTRYAG 2 cut(s) 672, 882
Sfr274I CTCGAG 1 cut(s) 1171
SinI GGWCC 1 cut(s) 932
SlaI CTCGAG 1 cut(s) 1171
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 4 cut(s) 485, 526, 1171, 1319
SmoI CTYRAG 4 cut(s) 485, 526, 1171, 1319
SnaBI TACGTA 1 cut(s) 1146
SpeI ACTAGT 1 cut(s) 601
SsiI CCGC 6 cut(s) 112, 180, 188, 366, 678, 1326
SspI AATATT 1 cut(s) 824
SspMI CTAG 2 cut(s) 204, 602
StyD4I CCNGG 6 cut(s) 30, 337, 559, 758, 1006, 1199
TaaI ACNGT 2 cut(s) 898, 1061
TaiI ACGT 4 cut(s) 126, 850, 1148, 1236
TaqI TCGA 4 cut(s) 216, 1096, 1172, 1383
TauI GCSGC 3 cut(s) 183, 191, 680
TfiI GAWTC 4 cut(s) 99, 956, 1011, 1292
Tru1I TTAA 5 cut(s) 795, 819, 861, 1116, 1263
Tru9I TTAA 5 cut(s) 795, 819, 861, 1116, 1263
TscAI CASTG 2 cut(s) 465, 1431
TseI GCWGC 4 cut(s) 200, 671, 674, 1034
TspDTI ATGAA 5 cut(s) 335, 435, 557, 790, 1203
TspGWI ACGGA 3 cut(s) 31, 237, 640
TspRI CASTG 2 cut(s) 465, 1431
Van91I CCANNNNNTGG 1 cut(s) 1284
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 932
XapI RAATTY 5 cut(s) 469, 773, 797, 1258, 1340
XceI RCATGY 1 cut(s) 1082
XhoI CTCGAG 1 cut(s) 1171
XmiI GTMKAC 1 cut(s) 973
XspI CTAG 2 cut(s) 204, 602
Zsp2I ATGCAT 1 cut(s) 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.