Rh7AG159400

Monodehydroascorbate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005394
Physical Location & Seq
Reverse (-)
13341295 .. 13346144
4850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG159400.1

Sequence Viewer

Length: 1362 bp
ATGGCCTCCGCCATCGCCAACTCTCTCTCCTTCAAGCTCGGCGTTCCTCTCCGCCCTCATCCTCACCCCACTCACACCTCCGCTTCACTCGGATTCACAACCTTCCGCCGAAGCTACGTCGTTTCGTCCTCTGCTTTCGCCAATCAAAACCGCGAGTATGTGATTGTTGGTGGTGGCAATGCTGCTGGCTACGCGGCTAGGACTTTCGTCGAAAATGGAATGGCCGATGGGCGCCTTGCTATTGTATCTAAAGAGGCTCATGCGCCTTATGAGCGTCCAGCTCTGACTAAAGCGTATTTGTTCCCATTAGATAAGAAGCCGGCTCGCTTACCTGGGTTTCATACTTGTGTTGGATCTGGTGGGGAGAGGCAAACACCTGACTGGTATAAAGAAAAAGGGATAGAGATGTTCTATGAAGATCCGGTTACAAATGTTGATATCGAAAAGCAAACCCTGACTACAGATTCAGGGAAACTGGTTAAGTATGGATCACTTATCATTGCTACAGGATGCACAGCCTCAAGATTGCCAGAGAAAATAGGAGGAAACCTACCTGGTGTTCATTATATCCGGGATGTTGCTGATGCTGATGCACTTATATCATCGTTGGAGAAAGCACAGAAGGTGGTCATTGTCGGTGGTGGTTATATTGGTATGGAGGTTGCTGCAGCAGCTACTGGTTGGAAACTTGATACAACAATCATATTTCCAGAGAACCATCTTATGTCAAGACTGTTTACTCCTTCACTTGCCCGGAGATATGAAGAATTCTACAAAGAGAATGGTGTTAAATTCTTAAAGGGTAACTCCCTAAAGAATATAGAAGCTGGTTCCAATGGACGTGTAACTCATGTTAAGCTTGAAGATGGATCTAGTATAGAAGCAGACGCAGTAATTGTTGGTATAGGAGCAAAACCTGCTGTCGGTCCTTTTGAAAGAGTGGGTTTAGATTCCAGTGTTGGTGGTATACAGGTTGATGGTCTGTTCCGGACAAGTGTACCTGGAATCTTTGCAGTCGGAGATGTTGCAGCATTCCCATTGAAGATCTACAATCGTATAGCACGGGTGGAACATGTAGATCATGCTCGTCGATCTGCCCAGCATTGCATTAAAGCTCTACTGACTGCACAAACTCACACATATGATTATCTTCCATCTTTCTACTCGAGGGTATTTGAGTATGAAGGAAGCCCGAGGAAAATATGGTGGCAATTTTATGGGGACAATGTTGGAGAGACGGTTGAAGTTGGGAATTTTGATCCTAAGATTGCAACTTTCTGGATAGATTCTGGTAAACTGAAAGGTGTTCTCGTTGAAAGCGGAAGCCCTGAGGTACTTTCTTTGTATGTTTTATTTCTTTAG

Protein Analysis

453

Amino Acids

49.3

Weight (kDa)

8.21

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 52 - 365 4e-58 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 131 - 340 1.5e-07 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 208 - 290 3.6e-18 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 384 - 444 2.6e-12 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 925
AccB1I GGYRCC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 967
AccII CGCG 2 cut(s) 153, 194
AccIII TCCGGA 1 cut(s) 987
AciI CCGC 7 cut(s) 9, 52, 81, 106, 151, 194, 1320
AclWI GGATC 5 cut(s) 361, 413, 496, 877, 1253
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 3 cut(s) 767, 791, 1252
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 2 cut(s) 999, 1335
AfiI CCNNNNNNNGG 1 cut(s) 923
AflIII ACRYGT 2 cut(s) 841, 1072
AgsI TTSAA 6 cut(s) 34, 863, 935, 1042, 1244, 1316
AjiI CACGTC 1 cut(s) 842
AjnI CCWGG 3 cut(s) 331, 553, 1000
AluBI AGCT 7 cut(s) 37, 114, 281, 674, 827, 859, 1115
AluI AGCT 7 cut(s) 37, 114, 281, 674, 827, 859, 1115
Alw26I GTCTC 1 cut(s) 1229
AlwI GGATC 5 cut(s) 361, 413, 496, 877, 1253
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 2 cut(s) 1165, 1192
Aor13HI TCCGGA 1 cut(s) 987
AoxI GGCC 2 cut(s) 3, 222
ApeKI GCWGC 5 cut(s) 182, 665, 668, 671, 1028
ApoI RAATTY 3 cut(s) 767, 791, 1252
AspLEI GCGC 2 cut(s) 234, 265
AspS9I GGNCC 1 cut(s) 926
AsuC2I CCSGG 2 cut(s) 572, 754
AsuHPI GGTGA 1 cut(s) 56
AvaI CYCGRG 2 cut(s) 1165, 1192
AvaII GGWCC 1 cut(s) 926
AxyI CCTNAGG 1 cut(s) 1329
BaeI ACNNNNGTAYC 2 cut(s) 981, 1014
BanI GGYRCC 1 cut(s) 231
BbvI GCAGC 5 cut(s) 169, 652, 680, 683, 1040
BccI CCATC 6 cut(s) 20, 221, 726, 860, 971, 1162
BciT130I CCWGG 3 cut(s) 333, 555, 1002
BcnI CCSGG 2 cut(s) 572, 754
BcoDI GTCTC 1 cut(s) 1229
BfaI CTAG 2 cut(s) 198, 873
BfmI CTRYAG 3 cut(s) 459, 504, 666
BfoI RGCGCY 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 925
BglII AGATCT 1 cut(s) 1044
BisI GCNGC 6 cut(s) 183, 195, 666, 669, 672, 1029
BlsI GCNGC 6 cut(s) 184, 196, 667, 670, 673, 1030
Bme1390I CCNGG 5 cut(s) 333, 555, 572, 754, 1002
Bme18I GGWCC 1 cut(s) 926
BmeT110I CYCGRG 2 cut(s) 1165, 1192
BmgBI CACGTC 1 cut(s) 842
BmgT120I GGNCC 1 cut(s) 926
BmiI GGNNCC 2 cut(s) 233, 832
BmrFI CCNGG 5 cut(s) 333, 555, 572, 754, 1002
BmsI GCATC 3 cut(s) 500, 574, 580
BoxI GACNNNNGTC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 2 cut(s) 572, 754
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 332, 1193
BsaWI WCCGGW 2 cut(s) 421, 987
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 1224, 1254
Bsc4I CCNNNNNNNGG 1 cut(s) 923
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 4 cut(s) 386, 480, 682, 954
Bse21I CCTNAGG 1 cut(s) 1329
Bse3DI GCAATG 3 cut(s) 184, 498, 1102
BseAI TCCGGA 1 cut(s) 987
BseBI CCWGG 3 cut(s) 333, 555, 1002
BseDI CCNNGG 2 cut(s) 332, 1193
BseGI GGATG 3 cut(s) 58, 515, 580
BseLI CCNNNNNNNGG 1 cut(s) 923
BseMI GCAATG 3 cut(s) 184, 498, 1102
BseMII CTCAG 1 cut(s) 1320
BseNI ACTGG 4 cut(s) 386, 480, 682, 954
BseXI GCAGC 5 cut(s) 169, 652, 680, 683, 1040
BseYI CCCAGC 1 cut(s) 1098
BsgI GTGCAG 1 cut(s) 1110
Bsh1236I CGCG 2 cut(s) 153, 194
BshFI GGCC 2 cut(s) 5, 224
BshNI GGYRCC 1 cut(s) 231
BsiHKCI CYCGRG 2 cut(s) 1165, 1192
BsiSI CCGG 5 cut(s) 320, 422, 571, 754, 988
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 1 cut(s) 923
BsmAI GTCTC 1 cut(s) 1229
BsmBI CGTCTC 1 cut(s) 1229
BsmFI GGGAC 1 cut(s) 1235
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 2 cut(s) 5, 224
BsoBI CYCGRG 2 cut(s) 1165, 1192
Bsp13I TCCGGA 1 cut(s) 987
Bsp143I GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
BspACI CCGC 7 cut(s) 9, 52, 81, 106, 151, 194, 1320
BspANI GGCC 2 cut(s) 5, 224
BspCNI CTCAG 1 cut(s) 1321
BspEI TCCGGA 1 cut(s) 987
BspFNI CGCG 2 cut(s) 153, 194
BspLI GGNNCC 2 cut(s) 233, 832
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 1 cut(s) 925
BspPI GGATC 5 cut(s) 361, 413, 496, 877, 1253
BspT107I GGYRCC 1 cut(s) 231
BsrDI GCAATG 3 cut(s) 184, 498, 1102
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 4 cut(s) 386, 480, 682, 954
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 332, 1193
BssMI GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
BssNAI GTATAC 1 cut(s) 968
BssNI GRCGYC 1 cut(s) 232
Bst1107I GTATAC 1 cut(s) 968
Bst2UI CCWGG 3 cut(s) 333, 555, 1002
Bst4CI ACNGT 2 cut(s) 735, 1240
BstACI GRCGYC 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 917
BstC8I GCNNGC 3 cut(s) 187, 321, 325
BstDEI CTNAG 2 cut(s) 1263, 1329
BstF5I GGATG 3 cut(s) 58, 515, 580
BstFNI CGCG 2 cut(s) 153, 194
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 2 cut(s) 234, 265
BstKTI GATC 8 cut(s) 356, 421, 491, 872, 1047, 1081, 1094, 1261
BstMAI GTCTC 1 cut(s) 1229
BstMBI GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 191, 271, 671, 917
BstNI CCWGG 3 cut(s) 333, 555, 1002
BstNSI RCATGY 1 cut(s) 1076
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 5 cut(s) 331, 553, 570, 752, 1000
BstSFI CTRYAG 3 cut(s) 459, 504, 666
BstUI CGCG 2 cut(s) 153, 194
BstV1I GCAGC 5 cut(s) 169, 652, 680, 683, 1040
BstX2I RGATCY 4 cut(s) 353, 418, 869, 1044
BstYI RGATCY 4 cut(s) 353, 418, 869, 1044
BstZ17I GTATAC 1 cut(s) 968
Bsu36I CCTNAGG 1 cut(s) 1329
BsuRI GGCC 2 cut(s) 5, 224
BtrI CACGTC 1 cut(s) 842
BtsCI GGATG 3 cut(s) 58, 515, 580
BtsIMutI CAGTG 1 cut(s) 961
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 3 cut(s) 187, 321, 325
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 2 cut(s) 234, 265
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 926
CseI GACGC 2 cut(s) 263, 896
CsiI ACCWGGT 1 cut(s) 553
Csp6I GTAC 2 cut(s) 998, 1334
CviAII CATG 4 cut(s) 260, 851, 1073, 1082
CviQI GTAC 2 cut(s) 998, 1334
DdeI CTNAG 2 cut(s) 1263, 1329
DinI GGCGCC 1 cut(s) 233
DpnI GATC 8 cut(s) 355, 420, 490, 871, 1046, 1080, 1093, 1260
DpnII GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
EaeI YGGCCR 1 cut(s) 222
EciI GGCGGA 2 cut(s) 41, 95
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 926
Eco81I CCTNAGG 1 cut(s) 1329
Eco88I CYCGRG 2 cut(s) 1165, 1192
EcoRI GAATTC 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 331, 553, 1000
EcoRV GATATC 1 cut(s) 439
EgeI GGCGCC 1 cut(s) 233
EheI GGCGCC 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 1229
FaeI CATG 4 cut(s) 263, 854, 1076, 1085
FaqI GGGAC 1 cut(s) 1235
FatI CATG 4 cut(s) 259, 850, 1072, 1081
FauNDI CATATG 1 cut(s) 1141
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 6 cut(s) 183, 195, 666, 669, 672, 1029
FokI GGATG 3 cut(s) 45, 522, 587
Fsp4HI GCNGC 6 cut(s) 183, 195, 666, 669, 672, 1029
FspBI CTAG 2 cut(s) 198, 873
GlaI GCGC 2 cut(s) 233, 264
GluI GCNGC 6 cut(s) 183, 195, 666, 669, 672, 1029
GsaI CCCAGC 1 cut(s) 1102
HaeII RGCGCY 1 cut(s) 235
HaeIII GGCC 2 cut(s) 5, 224
HapII CCGG 5 cut(s) 320, 422, 571, 754, 988
HgaI GACGC 2 cut(s) 263, 896
HhaI GCGC 2 cut(s) 234, 265
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 4 cut(s) 263, 854, 1076, 1085
Hin6I GCGC 2 cut(s) 232, 263
HinP1I GCGC 2 cut(s) 232, 263
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 5 cut(s) 93, 464, 950, 1005, 1286
HpaII CCGG 5 cut(s) 320, 422, 571, 754, 988
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 4 cut(s) 738, 968, 998, 1295
Hpy188I TCNGA 3 cut(s) 92, 285, 1019
Hpy188III TCNNGA 5 cut(s) 522, 710, 729, 988, 1279
Hpy8I GTNNAC 4 cut(s) 738, 968, 998, 1295
Hpy99I CGWCG 3 cut(s) 122, 212, 1092
HpyAV CCTTC 5 cut(s) 40, 112, 616, 753, 1178
HpyCH4III ACNGT 2 cut(s) 735, 1240
HpyCH4IV ACGT 2 cut(s) 117, 841
HpyCH4V TGCA 8 cut(s) 513, 593, 668, 1013, 1028, 1107, 1127, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 271, 671, 917
HpyF3I CTNAG 2 cut(s) 1263, 1329
HpySE526I ACGT 2 cut(s) 117, 841
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 4 cut(s) 263, 854, 1076, 1085
HspAI GCGC 2 cut(s) 232, 263
KasI GGCGCC 1 cut(s) 231
Kpn2I TCCGGA 1 cut(s) 987
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
LmnI GCTCC 1 cut(s) 908
Lsp1109I GCAGC 5 cut(s) 169, 652, 680, 683, 1040
LweI GCATC 3 cut(s) 500, 574, 580
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 2 cut(s) 198, 873
MaeII ACGT 2 cut(s) 117, 841
MaeIII GTNAC 3 cut(s) 424, 803, 844
MalI GATC 8 cut(s) 355, 420, 490, 871, 1046, 1080, 1093, 1260
MboI GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
MboII GAAGA 5 cut(s) 428, 776, 875, 1054, 1142
MflI RGATCY 4 cut(s) 353, 418, 869, 1044
MluCI AATT 5 cut(s) 767, 791, 894, 1211, 1252
Mly113I GGCGCC 1 cut(s) 232
MmeI TCCRAC 5 cut(s) 331, 588, 662, 997, 1210
MroI TCCGGA 1 cut(s) 987
MroNI GCCGGC 1 cut(s) 319
MseI TTAA 5 cut(s) 480, 789, 797, 855, 1110
MslI CAYNNNNRTG 2 cut(s) 345, 1140
MspI CCGG 5 cut(s) 320, 422, 571, 754, 988
MspR9I CCNGG 5 cut(s) 333, 555, 572, 754, 1002
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 3 cut(s) 333, 555, 1002
MvnI CGCG 2 cut(s) 153, 194
MwoI GCNNNNNNNGC 4 cut(s) 191, 271, 671, 917
NaeI GCCGGC 1 cut(s) 321
NarI GGCGCC 1 cut(s) 232
NciI CCSGG 2 cut(s) 572, 754
NdeI CATATG 1 cut(s) 1141
NdeII GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 4 cut(s) 263, 854, 1076, 1085
NlaIV GGNNCC 2 cut(s) 233, 832
NmeAIII GCCGAG 1 cut(s) 18
NspI RCATGY 1 cut(s) 1076
PaeR7I CTCGAG 1 cut(s) 1165
PciI ACATGT 1 cut(s) 1072
PctI GAATGC 1 cut(s) 1031
PdiI GCCGGC 1 cut(s) 321
PfeI GAWTC 5 cut(s) 93, 464, 950, 1005, 1286
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 6 cut(s) 184, 196, 667, 670, 673, 1030
PluTI GGCGCC 1 cut(s) 235
PscI ACATGT 1 cut(s) 1072
PshAI GACNNNNGTC 1 cut(s) 206
Psp6I CCWGG 3 cut(s) 331, 553, 1000
PspFI CCCAGC 1 cut(s) 1098
PspGI CCWGG 3 cut(s) 331, 553, 1000
PspN4I GGNNCC 2 cut(s) 233, 832
PspPI GGNCC 1 cut(s) 926
PspXI VCTCGAGB 1 cut(s) 1165
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 4 cut(s) 353, 418, 869, 1044
RsaI GTAC 2 cut(s) 999, 1335
RsaNI GTAC 2 cut(s) 998, 1334
RseI CAYNNNNRTG 2 cut(s) 345, 1140
SaqAI TTAA 5 cut(s) 480, 789, 797, 855, 1110
SatI GCNGC 6 cut(s) 183, 195, 666, 669, 672, 1029
Sau3AI GATC 8 cut(s) 353, 418, 488, 869, 1044, 1078, 1091, 1258
Sau96I GGNCC 1 cut(s) 926
ScrFI CCNGG 5 cut(s) 333, 555, 572, 754, 1002
SexAI ACCWGGT 1 cut(s) 553
SfaNI GCATC 3 cut(s) 500, 574, 580
SfcI CTRYAG 3 cut(s) 459, 504, 666
SfoI GGCGCC 1 cut(s) 233
Sfr274I CTCGAG 1 cut(s) 1165
SinI GGWCC 1 cut(s) 926
SlaI CTCGAG 1 cut(s) 1165
SmiMI CAYNNNNRTG 2 cut(s) 345, 1140
SmlI CTYRAG 2 cut(s) 520, 1165
SmoI CTYRAG 2 cut(s) 520, 1165
Sse9I AATT 5 cut(s) 767, 791, 894, 1211, 1252
SsiI CCGC 7 cut(s) 9, 52, 81, 106, 151, 194, 1320
SspDI GGCGCC 1 cut(s) 231
SspMI CTAG 2 cut(s) 198, 873
StyD4I CCNGG 5 cut(s) 331, 553, 570, 752, 1000
TaaI ACNGT 2 cut(s) 735, 1240
TaiI ACGT 2 cut(s) 120, 844
TaqI TCGA 4 cut(s) 210, 441, 1090, 1166
TaqII GACCGA 1 cut(s) 914
TasI AATT 5 cut(s) 767, 791, 894, 1211, 1252
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 5 cut(s) 93, 464, 950, 1005, 1286
Tru1I TTAA 5 cut(s) 480, 789, 797, 855, 1110
Tru9I TTAA 5 cut(s) 480, 789, 797, 855, 1110
TscAI CASTG 1 cut(s) 961
TseI GCWGC 5 cut(s) 182, 665, 668, 671, 1028
TspDTI ATGAA 5 cut(s) 329, 429, 551, 777, 1197
TspRI CASTG 1 cut(s) 961
VpaK11BI GGWCC 1 cut(s) 926
XapI RAATTY 3 cut(s) 767, 791, 1252
XceI RCATGY 1 cut(s) 1076
XhoI CTCGAG 1 cut(s) 1165
XmiI GTMKAC 1 cut(s) 967
XspI CTAG 2 cut(s) 198, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.