Rroxscaffold_3G00259130

Monodehydroascorbate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005394
Physical Location & Seq
Forward (+)
53552809 .. 53558089
5281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259130.1

Sequence Viewer

Length: 1392 bp
ATGTCTTCTTCAGCTACAGTACTCCGCACTGGACGAATGGCCTCCACCATCGCCAACTCTCTCTCCTTCAAGCTCGGCGTTCCTCTCCGCCCTCATCCTCACCCCACTCACGCCTCCGCTTCACTCGGATTCACAACCTTCCGCCGAAGCTACGTCGTTTCGTCCTCTGCTTTCGCCAATCAAAACCGCGAGTATGTGATTGTTGGTGGTGGCAATGCGGCTGGCTACGCGGCTAGGACTTTCGTCGAAAATGGAATGGCCGATGGGCGCCTTGCTATTGTGTCTAAAGAGGCTTATGCGCCTTACGAGCGTCCAGCTCTAACTAAAGCGTATTTGTTCCCATTAGATAAGAAGCCGGCTCGCTTACCTGGGTTTCATACCTGTGTTGGATCTGGTGGGGAGCGGCAAACACCTGACTGGTATAAAGAAAAAGGGATAGAGATGTTCTATGAAGATCCGGTTACAAATATTGATATCGAAAAGCAAACCCTGACTACAGATTCAGGGAAACTGCTTAAGTATGGATCACTTATAATTGCTACAGGATGCACGGCCTCAAGATTGCCAGAGAAAATAGGAGGAAACCTACCTGGTGTTCATTATATCCGGGATGTAGCCGATGCTGATGCACTTATATCATCGTTGGAGAAAGCACAGAAGGTGGTCATTGTCGGTGGTGGTTATATTGGTATGGAGGTTGCTGCAGCAGCTACTGGTTGGAAACTTGATACAACAATCATATTTCCAGAGAACCATCTTATGTCAAGACTGTTTACTCCTTCACTTGCCCAGAGATATGAAGAATTCTACAAAGAGAATGGTGTTAAATTCTTAAAGGTAATTGTTGGTATAGGAGCAAAACCTGCTGTCAGTCCTTTTGAAAGAGTGGGTTTAGATTCCAGTGTTGGTGGTATACAGGTTGATGGTCTGTTCCGGACAAGTGTACCTGGAATCTTTGCAGTTGGTGATGTTGCAGCATTCCCATTGAAGATCTACAATCGTATAGCACGGGTGGAACATGTAGATCATGCTCGTCGATCTGCCCAGCATTGCATTAAAGCTCTACTGACTGCACAAACTCACACATATGATTATCTTCCATCTTTCTACTCGAGGGTATTTGAGTATGAAGGCAGCCCGAGGAAAATATGGTGGCAATTTTATGGGGACAATGTTGGAGAGACAGTTGAAGTTGGGAATTTTGATCCTAAGATTGCAACTTTCTGGATAGATTCTGGTAAACTGAAAGGTGTTCTCGTTGAAAGCGGAAGCCCTGAGGAATTCCAACTCCTTCCAAACCTTGCAAGGAGCCAGCCTATTGTTGATAAAGCCATGCTTCAGAATGCATCTTCAGTTGAGGAGGCACTAGCCATTGTTGGGTCATCATTATAG

Protein Analysis

463

Amino Acids

50.43

Weight (kDa)

8.5

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 64 - 278 2.4e-34 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 220 - 278 5e-12 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 280 - 347 2e-09 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 366 - 444 4.4e-17 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 871
AccB1I GGYRCC 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 913
AccII CGCG 2 cut(s) 189, 230
AccIII TCCGGA 1 cut(s) 933
AciI CCGC 9 cut(s) 25, 88, 117, 142, 187, 218, 230, 403, 1266
AclWI GGATC 4 cut(s) 397, 449, 532, 1199
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 4 cut(s) 803, 827, 1198, 1280
AcuI CTGAAG 2 cut(s) 1322, 1335
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 2 cut(s) 21, 945
AfiI CCNNNNNNNGG 1 cut(s) 1377
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 5 cut(s) 70, 881, 988, 1190, 1262
AjnI CCWGG 3 cut(s) 367, 589, 946
AluBI AGCT 6 cut(s) 14, 73, 150, 317, 710, 1061
AluI AGCT 6 cut(s) 14, 73, 150, 317, 710, 1061
Alw26I GTCTC 1 cut(s) 1175
AlwI GGATC 4 cut(s) 397, 449, 532, 1199
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 2 cut(s) 1111, 1138
Aor13HI TCCGGA 1 cut(s) 933
AoxI GGCC 3 cut(s) 39, 258, 552
ApeKI GCWGC 5 cut(s) 701, 704, 707, 974, 1134
ApoI RAATTY 4 cut(s) 803, 827, 1198, 1280
AspLEI GCGC 2 cut(s) 270, 301
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 2 cut(s) 92, 977
AvaI CYCGRG 2 cut(s) 1111, 1138
AxyI CCTNAGG 1 cut(s) 1275
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BanI GGYRCC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 688, 716, 719, 986, 1146
BccI CCATC 5 cut(s) 56, 257, 762, 917, 1108
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 3 cut(s) 369, 591, 948
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 1175
BfaI CTAG 2 cut(s) 234, 1367
BfmI CTRYAG 4 cut(s) 15, 495, 540, 702
BfoI RGCGCY 1 cut(s) 271
BfrI CTTAAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 871
BglII AGATCT 1 cut(s) 990
BisI GCNGC 8 cut(s) 219, 231, 404, 702, 705, 708, 975, 1135
BlsI GCNGC 8 cut(s) 220, 232, 405, 703, 706, 709, 976, 1136
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 4 cut(s) 369, 591, 608, 948
BmeT110I CYCGRG 2 cut(s) 1111, 1138
BmiI GGNNCC 2 cut(s) 269, 1310
BmrFI CCNGG 4 cut(s) 369, 591, 608, 948
BmsI GCATC 4 cut(s) 536, 610, 616, 1355
BoxI GACNNNNGTC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 541
BpuMI CCSGG 1 cut(s) 608
BsaHI GRCGYC 1 cut(s) 268
BsaJI CCNNGG 2 cut(s) 368, 1139
BsaWI WCCGGW 2 cut(s) 457, 933
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 1170, 1200
Bsc4I CCNNNNNNNGG 1 cut(s) 1377
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 4 cut(s) 34, 422, 718, 900
Bse21I CCTNAGG 1 cut(s) 1275
Bse3DI GCAATG 2 cut(s) 220, 1048
BseAI TCCGGA 1 cut(s) 933
BseBI CCWGG 3 cut(s) 369, 591, 948
BseDI CCNNGG 2 cut(s) 368, 1139
BseGI GGATG 3 cut(s) 94, 551, 616
BseLI CCNNNNNNNGG 1 cut(s) 1377
BseMI GCAATG 2 cut(s) 220, 1048
BseMII CTCAG 1 cut(s) 1266
BseNI ACTGG 4 cut(s) 34, 422, 718, 900
BseRI GAGGAG 1 cut(s) 1373
BseXI GCAGC 5 cut(s) 688, 716, 719, 986, 1146
BseYI CCCAGC 1 cut(s) 1044
BsgI GTGCAG 1 cut(s) 1056
Bsh1236I CGCG 2 cut(s) 189, 230
BshFI GGCC 3 cut(s) 41, 260, 554
BshNI GGYRCC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 1111, 1138
BsiSI CCGG 4 cut(s) 356, 458, 607, 934
BslFI GGGAC 1 cut(s) 1181
BslI CCNNNNNNNGG 1 cut(s) 1377
BsmAI GTCTC 1 cut(s) 1175
BsmFI GGGAC 1 cut(s) 1181
BsmI GAATGC 2 cut(s) 977, 1348
BsnI GGCC 3 cut(s) 41, 260, 554
BsoBI CYCGRG 2 cut(s) 1111, 1138
Bsp13I TCCGGA 1 cut(s) 933
Bsp143I GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
BspACI CCGC 9 cut(s) 25, 88, 117, 142, 187, 218, 230, 403, 1266
BspANI GGCC 3 cut(s) 41, 260, 554
BspCNI CTCAG 1 cut(s) 1267
BspEI TCCGGA 1 cut(s) 933
BspFNI CGCG 2 cut(s) 189, 230
BspLI GGNNCC 2 cut(s) 269, 1310
BspMAI CTGCAG 1 cut(s) 706
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 4 cut(s) 397, 449, 532, 1199
BspT107I GGYRCC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 220, 1048
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 4 cut(s) 34, 422, 718, 900
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 368, 1139
BssMI GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
BssNAI GTATAC 1 cut(s) 914
BssNI GRCGYC 1 cut(s) 268
Bst1107I GTATAC 1 cut(s) 914
Bst2UI CCWGG 3 cut(s) 369, 591, 948
Bst4CI ACNGT 3 cut(s) 19, 771, 1186
BstACI GRCGYC 1 cut(s) 268
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 863
BstC8I GCNNGC 4 cut(s) 223, 357, 361, 1313
BstDEI CTNAG 2 cut(s) 1209, 1275
BstF5I GGATG 3 cut(s) 94, 551, 616
BstFNI CGCG 2 cut(s) 189, 230
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 2 cut(s) 270, 301
BstKTI GATC 7 cut(s) 392, 457, 527, 993, 1027, 1040, 1207
BstMAI GTCTC 1 cut(s) 1175
BstMBI GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
BstMWI GCNNNNNNNGC 4 cut(s) 227, 307, 707, 863
BstNI CCWGG 3 cut(s) 369, 591, 948
BstNSI RCATGY 1 cut(s) 1022
BstPAI GACNNNNGTC 1 cut(s) 242
BstSCI CCNGG 4 cut(s) 367, 589, 606, 946
BstSFI CTRYAG 4 cut(s) 15, 495, 540, 702
BstUI CGCG 2 cut(s) 189, 230
BstV1I GCAGC 5 cut(s) 688, 716, 719, 986, 1146
BstX2I RGATCY 3 cut(s) 389, 454, 990
BstYI RGATCY 3 cut(s) 389, 454, 990
BstZ17I GTATAC 1 cut(s) 914
Bsu36I CCTNAGG 1 cut(s) 1275
BsuRI GGCC 3 cut(s) 41, 260, 554
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 3 cut(s) 94, 551, 616
BtsIMutI CAGTG 2 cut(s) 27, 907
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 4 cut(s) 223, 357, 361, 1313
CaiI CAGNNNCTG 1 cut(s) 713
CfoI GCGC 2 cut(s) 270, 301
Cfr10I RCCGGY 1 cut(s) 355
CseI GACGC 1 cut(s) 299
CsiI ACCWGGT 1 cut(s) 589
Csp6I GTAC 2 cut(s) 20, 944
CviAII CATG 3 cut(s) 1019, 1028, 1333
CviQI GTAC 2 cut(s) 20, 944
DdeI CTNAG 2 cut(s) 1209, 1275
DinI GGCGCC 1 cut(s) 269
DpnI GATC 7 cut(s) 391, 456, 526, 992, 1026, 1039, 1206
DpnII GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
EaeI YGGCCR 1 cut(s) 258
EciI GGCGGA 2 cut(s) 77, 131
Eco32I GATATC 1 cut(s) 475
Eco57I CTGAAG 2 cut(s) 1322, 1335
Eco81I CCTNAGG 1 cut(s) 1275
Eco88I CYCGRG 2 cut(s) 1111, 1138
EcoRI GAATTC 2 cut(s) 803, 1280
EcoRII CCWGG 3 cut(s) 367, 589, 946
EcoRV GATATC 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 1348
EgeI GGCGCC 1 cut(s) 269
EheI GGCGCC 1 cut(s) 269
FaeI CATG 3 cut(s) 1022, 1031, 1336
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 1 cut(s) 1181
FatI CATG 3 cut(s) 1018, 1027, 1332
FauNDI CATATG 1 cut(s) 1087
FblI GTMKAC 1 cut(s) 913
Fnu4HI GCNGC 8 cut(s) 219, 231, 404, 702, 705, 708, 975, 1135
FokI GGATG 3 cut(s) 81, 558, 623
Fsp4HI GCNGC 8 cut(s) 219, 231, 404, 702, 705, 708, 975, 1135
FspBI CTAG 2 cut(s) 234, 1367
GlaI GCGC 2 cut(s) 269, 300
GluI GCNGC 8 cut(s) 219, 231, 404, 702, 705, 708, 975, 1135
GsaI CCCAGC 1 cut(s) 1048
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 3 cut(s) 41, 260, 554
HapII CCGG 4 cut(s) 356, 458, 607, 934
HgaI GACGC 1 cut(s) 299
HhaI GCGC 2 cut(s) 270, 301
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 3 cut(s) 1022, 1031, 1336
Hin6I GCGC 2 cut(s) 268, 299
HinP1I GCGC 2 cut(s) 268, 299
HinfI GANTC 5 cut(s) 129, 500, 896, 951, 1232
HpaII CCGG 4 cut(s) 356, 458, 607, 934
HphI GGTGA 2 cut(s) 92, 977
Hpy166II GTNNAC 4 cut(s) 774, 914, 944, 1241
Hpy188I TCNGA 2 cut(s) 128, 1341
Hpy188III TCNNGA 5 cut(s) 558, 746, 765, 934, 1225
Hpy8I GTNNAC 4 cut(s) 774, 914, 944, 1241
Hpy99I CGWCG 3 cut(s) 158, 248, 1038
HpyAV CCTTC 6 cut(s) 76, 148, 652, 789, 1124, 1301
HpyCH4III ACNGT 3 cut(s) 19, 771, 1186
HpyCH4IV ACGT 1 cut(s) 153
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 307, 707, 863
HpyF3I CTNAG 2 cut(s) 1209, 1275
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 3 cut(s) 1022, 1031, 1336
HspAI GCGC 2 cut(s) 268, 299
KasI GGCGCC 1 cut(s) 267
Kpn2I TCCGGA 1 cut(s) 933
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Kzo9I GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
LmnI GCTCC 3 cut(s) 400, 854, 1308
Lsp1109I GCAGC 5 cut(s) 688, 716, 719, 986, 1146
LweI GCATC 4 cut(s) 536, 610, 616, 1355
MabI ACCWGGT 1 cut(s) 589
MaeI CTAG 2 cut(s) 234, 1367
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 460
MalI GATC 7 cut(s) 391, 456, 526, 992, 1026, 1039, 1206
MbiI CCGCTC 1 cut(s) 403
MboI GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
MboII GAAGA 5 cut(s) 464, 812, 1000, 1088, 1341
MflI RGATCY 3 cut(s) 389, 454, 990
MluCI AATT 7 cut(s) 534, 803, 827, 840, 1157, 1198, 1280
Mly113I GGCGCC 1 cut(s) 268
MmeI TCCRAC 5 cut(s) 367, 624, 698, 1156, 1309
Mph1103I ATGCAT 1 cut(s) 1348
MroI TCCGGA 1 cut(s) 933
MroNI GCCGGC 1 cut(s) 355
MseI TTAA 4 cut(s) 516, 825, 833, 1056
MslI CAYNNNNRTG 2 cut(s) 381, 1086
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 4 cut(s) 356, 458, 607, 934
MspR9I CCNGG 4 cut(s) 369, 591, 608, 948
Mva1269I GAATGC 2 cut(s) 977, 1348
MvaI CCWGG 3 cut(s) 369, 591, 948
MvnI CGCG 2 cut(s) 189, 230
MwoI GCNNNNNNNGC 4 cut(s) 227, 307, 707, 863
NaeI GCCGGC 1 cut(s) 357
NarI GGCGCC 1 cut(s) 268
NciI CCSGG 1 cut(s) 608
NdeI CATATG 1 cut(s) 1087
NdeII GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 3 cut(s) 1022, 1031, 1336
NlaIV GGNNCC 2 cut(s) 269, 1310
NmeAIII GCCGAG 1 cut(s) 54
NsiI ATGCAT 1 cut(s) 1348
NspI RCATGY 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 1111
PciI ACATGT 1 cut(s) 1018
PctI GAATGC 2 cut(s) 977, 1348
PdiI GCCGGC 1 cut(s) 357
PfeI GAWTC 5 cut(s) 129, 500, 896, 951, 1232
PfoI TCCNGGA 1 cut(s) 606
PkrI GCNGC 8 cut(s) 220, 232, 405, 703, 706, 709, 976, 1136
PluTI GGCGCC 1 cut(s) 271
PscI ACATGT 1 cut(s) 1018
PshAI GACNNNNGTC 1 cut(s) 242
PsiI TTATAA 1 cut(s) 533
Psp6I CCWGG 3 cut(s) 367, 589, 946
PspFI CCCAGC 1 cut(s) 1044
PspGI CCWGG 3 cut(s) 367, 589, 946
PspN4I GGNNCC 2 cut(s) 269, 1310
PspXI VCTCGAGB 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 3 cut(s) 389, 454, 990
RsaI GTAC 2 cut(s) 21, 945
RsaNI GTAC 2 cut(s) 20, 944
RseI CAYNNNNRTG 2 cut(s) 381, 1086
SaqAI TTAA 4 cut(s) 516, 825, 833, 1056
SatI GCNGC 8 cut(s) 219, 231, 404, 702, 705, 708, 975, 1135
Sau3AI GATC 7 cut(s) 389, 454, 524, 990, 1024, 1037, 1204
ScaI AGTACT 1 cut(s) 21
ScrFI CCNGG 4 cut(s) 369, 591, 608, 948
SexAI ACCWGGT 1 cut(s) 589
SfaNI GCATC 4 cut(s) 536, 610, 616, 1355
SfcI CTRYAG 4 cut(s) 15, 495, 540, 702
SfoI GGCGCC 1 cut(s) 269
Sfr274I CTCGAG 1 cut(s) 1111
SlaI CTCGAG 1 cut(s) 1111
SmiMI CAYNNNNRTG 2 cut(s) 381, 1086
SmlI CTYRAG 3 cut(s) 515, 556, 1111
SmoI CTYRAG 3 cut(s) 515, 556, 1111
Sse9I AATT 7 cut(s) 534, 803, 827, 840, 1157, 1198, 1280
SsiI CCGC 9 cut(s) 25, 88, 117, 142, 187, 218, 230, 403, 1266
SspDI GGCGCC 1 cut(s) 267
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 234, 1367
StyD4I CCNGG 4 cut(s) 367, 589, 606, 946
TaaI ACNGT 3 cut(s) 19, 771, 1186
TaiI ACGT 1 cut(s) 156
TaqI TCGA 4 cut(s) 246, 477, 1036, 1112
TasI AATT 7 cut(s) 534, 803, 827, 840, 1157, 1198, 1280
TatI WGTACW 1 cut(s) 19
TauI GCSGC 3 cut(s) 221, 233, 406
TfiI GAWTC 5 cut(s) 129, 500, 896, 951, 1232
Tru1I TTAA 4 cut(s) 516, 825, 833, 1056
Tru9I TTAA 4 cut(s) 516, 825, 833, 1056
TscAI CASTG 2 cut(s) 34, 907
TseI GCWGC 5 cut(s) 701, 704, 707, 974, 1134
TspDTI ATGAA 5 cut(s) 365, 465, 587, 813, 1143
TspRI CASTG 2 cut(s) 34, 907
Vha464I CTTAAG 1 cut(s) 515
XapI RAATTY 4 cut(s) 803, 827, 1198, 1280
XceI RCATGY 1 cut(s) 1022
XhoI CTCGAG 1 cut(s) 1111
XmiI GTMKAC 1 cut(s) 913
XspI CTAG 2 cut(s) 234, 1367
ZrmI AGTACT 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.