pycom14g10710

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0018098
Physical Location & Seq
Reverse (-)
13444620 .. 13445269
650 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGAATTATGACTGTGATAAAGCCAACTCCAAAGCCACGAAGTGGGTATTATCTACTGACGGAACGATCACCAATCCTCACTCTGGGCTAGTCTTGACAGCCCATGGAGCTGAAAACGGCACCTCCCTAACAGCAGAAACCAATGTCAATGCTGCTACGCATGGTTGGAAAGCTGAAGATGACGTAGAGCCCACCGTCACTTCCATTGAGGGCTCTGAAGAACCCATCGTCATTCGCAAATTAGAAGGGTCAGTTAACCTGCGATGGGTGTTCAAGTACGATGGGACAATTCTAAACCCTAATGCTACACTTTTCATGGATGAGAGAGATAGCAATCAATCTCTTGAACAGATCATACTGTATCCTCGCACTGGGAATCCTTACCAGCAGTGGGTCTCATTTTTCTCAGTGCATGGTTGCCATGCATCCGTAGAGAAGAAATTATCCATTACTACCTTGTTTTCCTGCCATAAGCAATCAGTATATATAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RIP Ricin_B_lectin

Protein Analysis

165

Amino Acids

18.15

Weight (kDa)

5.44

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ricin_B_lectin PF00652 72 - 131 2.1e-06 Ricin-type beta-trefoil lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 42
AcuI CTGAAG 2 cut(s) 195, 237
AdeI CACNNNGTG 1 cut(s) 42
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 5 cut(s) 42, 83, 265, 371, 391
AgsI TTSAA 2 cut(s) 274, 347
AluBI AGCT 2 cut(s) 110, 173
AluI AGCT 2 cut(s) 110, 173
Alw26I GTCTC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 61
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 192, 215
BbvI GCAGC 1 cut(s) 139
BccI CCATC 3 cut(s) 233, 258, 275
BceAI ACGGC 1 cut(s) 133
BciVI GTATCC 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 267
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
BmiI GGNNCC 1 cut(s) 121
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 434
BmuI ACTGGG 1 cut(s) 381
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 83, 265, 371, 391
Bse1I ACTGG 1 cut(s) 376
Bse8I GATNNNNATC 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 2 cut(s) 325, 425
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 5 cut(s) 42, 83, 265, 371, 391
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 119
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 5 cut(s) 42, 83, 265, 371, 391
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 298
Bso31I GGTCTC 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 192, 215
Bsp143I GATC 2 cut(s) 66, 351
Bsp19I CCATGG 1 cut(s) 103
BspCNI CTCAG 1 cut(s) 419
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 400
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 2 cut(s) 66, 351
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 3 cut(s) 14, 196, 360
BstDEI CTNAG 1 cut(s) 406
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 325, 425
BstKTI GATC 2 cut(s) 69, 354
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 2 cut(s) 66, 351
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 139
BsuI GTATCC 1 cut(s) 372
BtgI CCRYGG 1 cut(s) 103
BtgZI GCGATG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 325, 425
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 3 cut(s) 369, 395, 414
BveI ACCTGC 1 cut(s) 267
Csp6I GTAC 1 cut(s) 277
CviAII CATG 5 cut(s) 104, 161, 316, 413, 422
CviJI RGCY 8 cut(s) 23, 35, 88, 101, 110, 173, 190, 213
CviKI_1 RGCY 8 cut(s) 23, 35, 88, 101, 110, 173, 190, 213
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 1 cut(s) 406
DpnI GATC 2 cut(s) 68, 353
DpnII GATC 2 cut(s) 66, 351
DraIII CACNNNGTG 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 2 cut(s) 192, 215
Eco31I GGTCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 195, 237
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 192, 215
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 5 cut(s) 107, 164, 319, 416, 425
FaqI GGGAC 1 cut(s) 298
FatI CATG 5 cut(s) 103, 160, 315, 412, 421
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 2 cut(s) 332, 412
FriOI GRGCYC 2 cut(s) 192, 215
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 1 cut(s) 89
GluI GCNGC 1 cut(s) 153
Hin1II CATG 5 cut(s) 107, 164, 319, 416, 425
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 1 cut(s) 376
HpaI GTTAAC 1 cut(s) 256
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 217
Hpy188III TCNNGA 2 cut(s) 94, 344
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 3 cut(s) 14, 196, 360
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 2 cut(s) 412, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 406
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 5 cut(s) 107, 164, 319, 416, 425
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 66, 351
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 5 cut(s) 69, 272, 357, 398, 478
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 196
MalI GATC 2 cut(s) 68, 353
MboI GATC 2 cut(s) 66, 351
MboII GAAGA 3 cut(s) 188, 230, 448
MhlI GDGCHC 2 cut(s) 192, 215
MluCI AATT 4 cut(s) 4, 239, 288, 440
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 4 cut(s) 87, 133, 202, 375
Mph1103I ATGCAT 1 cut(s) 427
MseI TTAA 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 107
NcoI CCATGG 1 cut(s) 103
NdeII GATC 2 cut(s) 66, 351
NlaIII CATG 5 cut(s) 107, 164, 319, 416, 425
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 427
PfeI GAWTC 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 42
PkrI GCNGC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 121
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 2 cut(s) 66, 351
SduI GDGCHC 2 cut(s) 192, 215
SetI ASST 6 cut(s) 112, 125, 175, 186, 261, 458
SfaNI GCATC 1 cut(s) 434
Sse9I AATT 4 cut(s) 4, 239, 288, 440
SspMI CTAG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 3 cut(s) 14, 196, 360
TaiI ACGT 1 cut(s) 186
TasI AATT 4 cut(s) 4, 239, 288, 440
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 3 cut(s) 376, 395, 414
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 17, 304
TspGWI ACGGA 2 cut(s) 75, 418
TspRI CASTG 3 cut(s) 376, 395, 414
Van91I CCANNNNNTGG 1 cut(s) 42
XspI CTAG 1 cut(s) 89
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.