pycom14g11720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015303
Physical Location & Seq
Forward (+)
14801348 .. 14801920
573 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGCAAAACCTCGCCTACAAATCAAGCTCACAAACCCAGCAACAATGACAAACGAGTCAATCAAACCCAGCAGCCACTCCTCAGCCGATCACGGAGGCAGCAGGCACAACAAGCAACTGGACAAAGAAATCCGAGACATGGTCTCCGCCATCACCAACCGAGTCACGGACATCCAAAAGTCTGGCTCCATCCACCACGAGGACGAGGACGAAGACGGTGTCAGGATCATCACCCTTGCAGGATCCAACACCGGAGCTACATTGAGGGGTGAGCTGGATCATGAGAACGTTAACAAACTTGCAGGGCTTCATGGTGGTGGTCCGGCTTTGGGAGAGGCTGATGGGCTGAGCACTTACGTGAACAGCAACTTCCAGGCCGTGAACAACTCCCTCATGATGGGCGGCAGCTACACCACGAATGACCCCGGCGTTTGCATGGACATCACAGATGTTGTGGAGCTGCATGGCGGGCATCATGGGAAGCCGGAGAAGCGGGGATGGAAGGGGAAGAACAAGGAGAAGGAGAAGGAGAGGGAGAGCTTACATAGCGACCAGCATCATGATACGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.63

Weight (kDa)

5.98

Isoelectric Point (pI)

24.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63310
fragaria_vesca FvH4_5g10310
malus_domestica MD00G1118700.v1.1 MD06G1125100.v1.1
prunus_persica Prupe.5G135700_v2.0.a1
pyrus_communis pycom14g11720
rosa_chinensis RchiOBHm_Chr7g0187911
rosa_laevigata RLG00000004748
rosa_multiflora Rmu_co8094926.1_g000001
rosa_roxburghii Rroxscaffold_3G00266950
rosa_rugosa Rorug06G0485300
rosa_samantha Rh7AG090500 Rh7BG092100 Rh7CG091200 Rh7DG092900
rosa_wichuraiana Rw7G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 55
AciI CCGC 4 cut(s) 147, 402, 468, 493
AclI AACGTT 1 cut(s) 288
AclWI GGATC 4 cut(s) 233, 237, 250, 285
AfiI CCNNNNNNNGG 5 cut(s) 139, 166, 199, 329, 397
AjnI CCWGG 1 cut(s) 372
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 6 cut(s) 28, 257, 274, 408, 460, 540
AluI AGCT 6 cut(s) 28, 257, 274, 408, 460, 540
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 2 cut(s) 129, 148
AlwI GGATC 4 cut(s) 233, 237, 250, 285
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 4 cut(s) 72, 99, 405, 460
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 145, 223, 281
AvaII GGWCC 1 cut(s) 320
BamHI GGATCC 1 cut(s) 242
BauI CACGAG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 4 cut(s) 84, 111, 417, 447
BccI CCATC 5 cut(s) 158, 197, 335, 391, 492
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 2 cut(s) 129, 148
BisI GCNGC 5 cut(s) 73, 100, 403, 406, 461
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 5 cut(s) 74, 101, 404, 407, 462
Bme1390I CCNGG 2 cut(s) 374, 426
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 2 cut(s) 187, 244
BmrFI CCNGG 2 cut(s) 374, 426
BmsI GCATC 2 cut(s) 481, 565
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 82
Bpu1102I GCTNAGC 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 358
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 166, 199, 329, 397
Bse1I ACTGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 3 cut(s) 171, 189, 503
BseLI CCNNNNNNNGG 5 cut(s) 139, 166, 199, 329, 397
BseMII CTCAG 2 cut(s) 96, 338
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 4 cut(s) 84, 111, 417, 447
BseYI CCCAGC 2 cut(s) 37, 68
BshFI GGCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 4 cut(s) 252, 323, 426, 485
BslI CCNNNNNNNGG 5 cut(s) 139, 166, 199, 329, 397
BsmAI GTCTC 2 cut(s) 129, 148
BsnI GGCC 1 cut(s) 377
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 4 cut(s) 88, 225, 242, 277
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 4 cut(s) 147, 402, 468, 493
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 2 cut(s) 95, 339
BspHI TCATGA 3 cut(s) 280, 393, 559
BspLI GGNNCC 2 cut(s) 187, 244
BspPI GGATC 4 cut(s) 233, 237, 250, 285
BspTNI GGTCTC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 4 cut(s) 88, 225, 242, 277
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 104, 470
BstDEI CTNAG 2 cut(s) 82, 347
BstF5I GGATG 3 cut(s) 171, 189, 503
BstKTI GATC 4 cut(s) 91, 228, 245, 280
BstMAI GTCTC 2 cut(s) 129, 148
BstMBI GATC 4 cut(s) 88, 225, 242, 277
BstMWI GCNNNNNNNGC 4 cut(s) 112, 469, 490, 546
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 372, 424
BstV1I GCAGC 4 cut(s) 84, 111, 417, 447
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 242
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 1 cut(s) 377
BtsCI GGATG 3 cut(s) 171, 189, 503
Cac8I GCNNGC 2 cut(s) 104, 470
CciI TCATGA 3 cut(s) 280, 393, 559
Cfr13I GGNCC 1 cut(s) 320
CviAII CATG 8 cut(s) 139, 281, 311, 394, 436, 464, 476, 560
DdeI CTNAG 2 cut(s) 82, 347
DpnI GATC 4 cut(s) 90, 227, 244, 279
DpnII GATC 4 cut(s) 88, 225, 242, 277
DrdI GACNNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 372
FaeI CATG 8 cut(s) 142, 284, 314, 397, 439, 467, 479, 563
FaiI YATR 9 cut(s) 140, 282, 312, 395, 437, 465, 477, 546, 561
FatI CATG 8 cut(s) 138, 280, 310, 393, 435, 463, 475, 559
FauI CCCGC 2 cut(s) 461, 486
Fnu4HI GCNGC 5 cut(s) 73, 100, 403, 406, 461
FokI GGATG 3 cut(s) 158, 176, 510
Fsp4HI GCNGC 5 cut(s) 73, 100, 403, 406, 461
GluI GCNGC 5 cut(s) 73, 100, 403, 406, 461
GsaI CCCAGC 2 cut(s) 41, 72
HaeIII GGCC 1 cut(s) 377
HapII CCGG 4 cut(s) 252, 323, 426, 485
Hin1II CATG 8 cut(s) 142, 284, 314, 397, 439, 467, 479, 563
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 56, 162
HpaI GTTAAC 1 cut(s) 292
HpaII CCGG 4 cut(s) 252, 323, 426, 485
HphI GGTGA 3 cut(s) 145, 223, 281
Hpy166II GTNNAC 3 cut(s) 292, 361, 382
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 4 cut(s) 223, 281, 394, 560
Hpy8I GTNNAC 3 cut(s) 292, 361, 382
HpyAV CCTTC 3 cut(s) 496, 514, 520
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 2 cut(s) 288, 357
HpyCH4V TGCA 4 cut(s) 239, 302, 435, 463
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 469, 490, 546
HpyF3I CTNAG 2 cut(s) 82, 347
HpySE526I ACGT 2 cut(s) 288, 357
Hsp92II CATG 8 cut(s) 142, 284, 314, 397, 439, 467, 479, 563
KspAI GTTAAC 1 cut(s) 292
Kzo9I GATC 4 cut(s) 88, 225, 242, 277
LmnI GCTCC 3 cut(s) 191, 254, 457
Lsp1109I GCAGC 4 cut(s) 84, 111, 417, 447
LweI GCATC 2 cut(s) 481, 565
MaeII ACGT 2 cut(s) 288, 357
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 90, 227, 244, 279
MboI GATC 4 cut(s) 88, 225, 242, 277
MboII GAAGA 2 cut(s) 224, 520
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 353
MlyI GAGTC 2 cut(s) 65, 171
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 9 cut(s) 21, 89, 91, 193, 199, 258, 328, 401, 525
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 2 cut(s) 315, 356
MspI CCGG 4 cut(s) 252, 323, 426, 485
MspR9I CCNGG 2 cut(s) 374, 426
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 4 cut(s) 112, 469, 490, 546
NciI CCSGG 1 cut(s) 426
NdeII GATC 4 cut(s) 88, 225, 242, 277
NlaIII CATG 8 cut(s) 142, 284, 314, 397, 439, 467, 479, 563
NlaIV GGNNCC 2 cut(s) 187, 244
NmuCI GTSAC 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 356
PagI TCATGA 3 cut(s) 280, 393, 559
PflFI GACNNNGTC 2 cut(s) 140, 218
PkrI GCNGC 5 cut(s) 74, 101, 404, 407, 462
PleI GAGTC 2 cut(s) 64, 170
PpsI GAGTC 2 cut(s) 64, 170
Ppu21I YACGTR 1 cut(s) 358
Psp1406I AACGTT 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 372
PspFI CCCAGC 2 cut(s) 37, 68
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 187, 244
PspPI GGNCC 1 cut(s) 320
PsuI RGATCY 1 cut(s) 242
PsyI GACNNNGTC 2 cut(s) 140, 218
RseI CAYNNNNRTG 2 cut(s) 315, 356
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 5 cut(s) 73, 100, 403, 406, 461
Sau3AI GATC 4 cut(s) 88, 225, 242, 277
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 2 cut(s) 65, 171
ScrFI CCNGG 2 cut(s) 374, 426
SduI GDGCHC 1 cut(s) 353
SetI ASST 9 cut(s) 13, 30, 259, 276, 291, 360, 410, 462, 542
SfaNI GCATC 2 cut(s) 481, 565
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 315, 356
SsiI CCGC 4 cut(s) 147, 402, 468, 493
StyD4I CCNGG 2 cut(s) 372, 424
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 2 cut(s) 291, 360
TauI GCSGC 1 cut(s) 405
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 4 cut(s) 72, 99, 405, 460
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 2 cut(s) 108, 182
Tth111I GACNNNGTC 2 cut(s) 140, 218
VpaK11BI GGWCC 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.