pycom14g13970

Leucine-rich repeat receptor-like serine threonine tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008551
Physical Location & Seq
Reverse (-)
16779759 .. 16781657
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g13970.1

Sequence Viewer

Length: 1899 bp
ATGGCAGCCTTATCCGGCAGCACCGCGCAGCTCTACCTCCTCACCTTCCTCTCCCTCCTCTTCCTCATCCACGCAAGACTCAATCTCGACCCTTCCGATCTCCAAGCCCTCTCCACCATCCAAAAAACCGTGGGCTTCCAATTCCATATAAGTCAACCAGCCCCATGCAATACTCCCGGTGTCTTCTGTGAGCGCAGGCTCTCAGACAACAACACCTACGTCCTCCGAATCACTCGGCTCGTCTTCAAATCCCACCGCCTCGACGGCTTTCTGAGCCCGGCAATAGGGCGGCTCTCCGAGCTCAAAGAGATCTCTCTTGCTCACAACAACCTCGGTGACCAAATCCCATCTCAAATCGTAGACTGCCGGAAGCTTGAGATTCTCAACTTCGGAGACAACCGGTTTTCAGGGGAAGTTCCTGCCGGGTTGTCTAACCTCATTCGCCTTCGCACCCTCGACCTTTCTTCTAACAAGCTTTCCGGGAATTTGAGCTTCCTCAAGCATTTTCCCAACCTGGAAAGCCTTTCCATTGCTGACAATTTCTTCTCTGGGAAAATCCCAGCTTCCATTCGTTCCTTTCGTAATCTTCGGTTATTCAACTTCGCTGGAAACGAATTCCTCGATGGCTCGTTACCAACGATGGAAAGCGTCGAGATTTCAGGAACCGATGTGCCAAAACGTTACATTTTGGCTGAGAATACAAGCACAAAAGGCCACAACAACACCACAGCTGCTGCTAATGCTCAAGCTCCAACTAATGCTCTAGCTCCAGGTCCATCTGCAACACCAAAGCACAAGAAGAAGAATAACACGAAGAAGGTAGGAGGGTGGCTGCTCGGATTTTTTGCCGGAGCACTGGCCGGATTTGTATCCGGGTTGATATTCTCTCTGCTGTTTAAACTGCTCTTGGCCGTGGTAAGAGGTGGCGGCAAAGACGGCGGCCCTGCGATTTTCAGTTCTCTGATCAAAAGTAAAGAGGACTTGGCTTTCCTGGAAAAAGAAGATGGCTTGGCTAATTTGGAACTCATAGGAAGCGGTGGATGTGGACAGGTTTATAAAGCCGAATTGCCAGGAAGCAACGGCAAAATGATTGCTATAAAGAAGATAATTCAACCCCCTAAAGATGCCGATGAACTGACGGCTGATGAGGAAAGTAAGCTTATGAACAAGAAGATGCGTCAAATCCGATCGGAGATCAACACTGTAGGGAAAATTAGGCACCGGAATCTTATTCCTCTGCTAGCCCACGTGTCTCGACTGGATTGTCATTACCTGGTCTATGAGTTCATGAAGAATGGGAGCCTGCAAGACATATTGAATCAAGTTGCGGACGGAACAAGACAGTTGGACTGGCTTACAAGGCATAAAATTGCACTGGGAATTGCTGCAGGGCTTGAATACCTTCACTTGAACAACACCCCTCACATAATTCACAGAGATCTCAAGCCAGCAAACGTCCTGCTCGATGATGATATGGAAGCTCGGATTGCGGACTTTGGGCTTGCAAAAGCAATGCCGGACGCGCATACTCATATTTCAACTTCGAATGTGATGGGAACTGTTGGATATATCGCACCAGAATACCATCAGACATTCAAGTTCACAGACAGGTGTGATATATACAGTTTCGGGGTGGTGTTGGGGGTTCTGGTGATGGGAAGGCTTCCATCCGACGAGTTTTTCCAGAACACGAACGAGATGAGTCTGGTTAAGTGGATGAGGAATGTCATGACTTCAGAGAAACCTAAGCAGGCAATCGACGAGAAATTGATAGGAAACGGATATGAGGAGCAAATGCTCCTGGTTCTCAAGATTGCTTGCTTTTGCACTTTGGATAACCCGAAAGAGAGGCCTAACAGTAAGGACGTTAGGTGTATGCTGGCTCAGATCAAGCACTAA

Protein Analysis

633

Amino Acids

69.77

Weight (kDa)

8.91

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 100 - 159 8.5e-07 Leucine rich repeat
LRR_4 PF12799 148 - 184 8.2e-06 Leucine Rich repeats (2 copies)
Pkinase PF00069 339 - 620 4.2e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 340 - 585 3.3e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AasI GACNNNNNNGTC 1 cut(s) 1263
AccB1I GGYRCC 1 cut(s) 1218
AccI GTMKAC 1 cut(s) 360
AccII CGCG 2 cut(s) 26, 1523
AciI CCGC 8 cut(s) 24, 256, 289, 927, 939, 1035, 1328, 1490
AclI AACGTT 1 cut(s) 679
AcoI YGGCCR 2 cut(s) 858, 909
AcsI RAATTY 2 cut(s) 484, 614
AcuI CTGAAG 1 cut(s) 1719
AcvI CACGTG 1 cut(s) 1249
AfiI CCNNNNNNNGG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 1248
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 8 cut(s) 247, 598, 1112, 1318, 1397, 1411, 1539, 1597
AjnI CCWGG 6 cut(s) 513, 769, 990, 1069, 1272, 1800
Alw21I GWGCWC 2 cut(s) 303, 856
Alw26I GTCTC 2 cut(s) 387, 1257
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 5 cut(s) 712, 858, 909, 940, 1850
ApeKI GCWGC 7 cut(s) 5, 18, 28, 731, 734, 832, 1385
ApoI RAATTY 2 cut(s) 484, 614
AsiGI ACCGGT 1 cut(s) 399
Asp700I GAANNNNTTC 1 cut(s) 1401
AspLEI GCGC 3 cut(s) 28, 195, 1525
AspS9I GGNCC 2 cut(s) 773, 941
AsuC2I CCSGG 5 cut(s) 177, 278, 424, 481, 874
AsuHPI GGTGA 3 cut(s) 34, 347, 1663
AsuII TTCGAA 1 cut(s) 1544
AsuNHI GCTAGC 1 cut(s) 1240
AvaII GGWCC 1 cut(s) 773
BanI GGYRCC 1 cut(s) 1218
BanII GRGCYC 2 cut(s) 278, 303
BbrPI CACGTG 1 cut(s) 1249
BbsI GAAGAC 2 cut(s) 175, 235
Bbv12I GWGCWC 2 cut(s) 303, 856
BbvI GCAGC 7 cut(s) 17, 30, 40, 718, 721, 819, 1372
BceAI ACGGC 5 cut(s) 280, 896, 952, 1096, 1155
BciT130I CCWGG 6 cut(s) 515, 771, 992, 1071, 1274, 1802
BciVI GTATCC 1 cut(s) 880
BclI TGATCA 1 cut(s) 963
BcnI CCSGG 5 cut(s) 177, 278, 424, 481, 874
BcoDI GTCTC 2 cut(s) 387, 1257
BfaI CTAG 2 cut(s) 764, 1241
BfmI CTRYAG 2 cut(s) 1203, 1386
BfuI GTATCC 1 cut(s) 880
BglI GCCNNNNNGGC 1 cut(s) 264
BglII AGATCT 2 cut(s) 309, 1438
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 2 cut(s) 773, 941
BmiI GGNNCC 3 cut(s) 664, 1220, 1301
BmrI ACTGGG 1 cut(s) 1385
BmsI GCATC 2 cut(s) 1114, 1164
BmtI GCTAGC 1 cut(s) 1244
BmuI ACTGGG 1 cut(s) 1385
BpiI GAAGAC 2 cut(s) 175, 235
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 1746
Bpu14I TTCGAA 1 cut(s) 1544
BpuEI CTTGAG 5 cut(s) 395, 482, 729, 1427, 1793
BpuMI CCSGG 5 cut(s) 177, 278, 424, 481, 874
BsaAI YACGTR 1 cut(s) 1249
BsaBI GATNNNNATC 1 cut(s) 868
BsaJI CCNNGG 3 cut(s) 129, 331, 912
BsaWI WCCGGW 2 cut(s) 399, 1221
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 4 cut(s) 861, 1263, 1355, 1380
Bse3DI GCAATG 2 cut(s) 528, 1518
Bse8I GATNNNNATC 1 cut(s) 868
BseBI CCWGG 6 cut(s) 515, 771, 992, 1071, 1274, 1802
BseDI CCNNGG 3 cut(s) 129, 331, 912
BseGI GGATG 5 cut(s) 66, 117, 1046, 1667, 1722
BseJI GATNNNNATC 1 cut(s) 868
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 2 cut(s) 528, 1518
BseMII CTCAG 4 cut(s) 216, 263, 684, 1898
BseNI ACTGG 4 cut(s) 861, 1263, 1355, 1380
BseRI GAGGAG 3 cut(s) 29, 47, 1802
BseXI GCAGC 7 cut(s) 17, 30, 40, 718, 721, 819, 1372
BseYI CCCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 26, 1523
Bsh1285I CGRYCG 1 cut(s) 1190
BshFI GGCC 5 cut(s) 714, 860, 911, 942, 1852
BshNI GGYRCC 1 cut(s) 1218
BshTI ACCGGT 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 1190
BsiHKAI GWGCWC 2 cut(s) 303, 856
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 2 cut(s) 387, 1257
BsnI GGCC 5 cut(s) 714, 860, 911, 942, 1852
Bsp119I TTCGAA 1 cut(s) 1544
Bsp1286I GDGCHC 3 cut(s) 278, 303, 856
Bsp143I GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
BspACI CCGC 8 cut(s) 24, 256, 289, 927, 939, 1035, 1328, 1490
BspANI GGCC 5 cut(s) 714, 860, 911, 942, 1852
BspCNI CTCAG 4 cut(s) 215, 264, 685, 1897
BspFNI CGCG 2 cut(s) 26, 1523
BspHI TCATGA 2 cut(s) 1287, 1728
BspLI GGNNCC 3 cut(s) 664, 1220, 1301
BspMAI CTGCAG 1 cut(s) 1390
BspOI GCTAGC 1 cut(s) 1244
BspT104I TTCGAA 1 cut(s) 1544
BspT107I GGYRCC 1 cut(s) 1218
BsrDI GCAATG 2 cut(s) 528, 1518
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 4 cut(s) 861, 1263, 1355, 1380
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 3 cut(s) 129, 331, 912
BssMI GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
Bst2UI CCWGG 6 cut(s) 515, 771, 992, 1071, 1274, 1802
Bst4CI ACNGT 6 cut(s) 130, 1204, 1344, 1561, 1625, 1859
Bst6I CTCTTC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 1249
BstBI TTCGAA 1 cut(s) 1544
BstC8I GCNNGC 8 cut(s) 197, 1242, 1304, 1449, 1503, 1752, 1819, 1881
BstDEI CTNAG 5 cut(s) 202, 272, 693, 1746, 1884
BstDSI CCRYGG 2 cut(s) 129, 912
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 5 cut(s) 66, 117, 1046, 1667, 1722
BstFNI CGCG 2 cut(s) 26, 1523
BstHHI GCGC 3 cut(s) 28, 195, 1525
BstKTI GATC 7 cut(s) 100, 312, 966, 1190, 1197, 1441, 1890
BstMAI GTCTC 2 cut(s) 387, 1257
BstMBI GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
BstMCI CGRYCG 1 cut(s) 1190
BstMWI GCNNNNNNNGC 9 cut(s) 264, 273, 298, 711, 740, 936, 1360, 1487, 1522
BstNI CCWGG 6 cut(s) 515, 771, 992, 1071, 1274, 1802
BstPI GGTNACC 1 cut(s) 335
BstSFI CTRYAG 2 cut(s) 1203, 1386
BstUI CGCG 2 cut(s) 26, 1523
BstV1I GCAGC 7 cut(s) 17, 30, 40, 718, 721, 819, 1372
BstV2I GAAGAC 2 cut(s) 175, 235
BstX2I RGATCY 2 cut(s) 309, 1438
BstYI RGATCY 2 cut(s) 309, 1438
BsuI GTATCC 1 cut(s) 880
BsuRI GGCC 5 cut(s) 714, 860, 911, 942, 1852
BtgI CCRYGG 2 cut(s) 129, 912
BtsCI GGATG 5 cut(s) 66, 117, 1046, 1667, 1722
BtsIMutI CAGTG 3 cut(s) 854, 1200, 1373
Cac8I GCNNGC 8 cut(s) 197, 1242, 1304, 1449, 1503, 1752, 1819, 1881
CaiI CAGNNNCTG 1 cut(s) 734
CciI TCATGA 2 cut(s) 1287, 1728
CfoI GCGC 3 cut(s) 28, 195, 1525
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 773, 941
CseI GACGC 3 cut(s) 637, 1166, 1529
CsiI ACCWGGT 1 cut(s) 1272
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 3 cut(s) 165, 1288, 1729
DdeI CTNAG 5 cut(s) 202, 272, 693, 1746, 1884
DpnI GATC 7 cut(s) 99, 311, 965, 1189, 1196, 1440, 1889
DpnII GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
DraI TTTAAA 1 cut(s) 898
DrdI GACNNNNNNGTC 1 cut(s) 1263
DseDI GACNNNNNNGTC 1 cut(s) 1263
EaeI YGGCCR 2 cut(s) 858, 909
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 301
Eco147I AGGCCT 1 cut(s) 1852
Eco24I GRGCYC 2 cut(s) 278, 303
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 1719
Eco72I CACGTG 1 cut(s) 1249
Eco91I GGTNACC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 6 cut(s) 513, 769, 990, 1069, 1272, 1800
EcoT38I GRGCYC 2 cut(s) 278, 303
FaeI CATG 3 cut(s) 168, 1291, 1732
FatI CATG 3 cut(s) 164, 1287, 1728
FbaI TGATCA 1 cut(s) 963
FblI GTMKAC 1 cut(s) 360
FokI GGATG 5 cut(s) 53, 104, 1053, 1654, 1729
FriOI GRGCYC 2 cut(s) 278, 303
FspBI CTAG 2 cut(s) 764, 1241
GlaI GCGC 3 cut(s) 27, 194, 1524
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 5 cut(s) 714, 860, 911, 942, 1852
HgaI GACGC 3 cut(s) 637, 1166, 1529
HhaI GCGC 3 cut(s) 28, 195, 1525
Hin1II CATG 3 cut(s) 168, 1291, 1732
Hin6I GCGC 3 cut(s) 26, 193, 1523
HinP1I GCGC 3 cut(s) 26, 193, 1523
HincII GTYRAC 1 cut(s) 155
HindII GTYRAC 1 cut(s) 155
HindIII AAGCTT 3 cut(s) 371, 473, 1157
HinfI GANTC 6 cut(s) 78, 228, 379, 1225, 1318, 1702
HphI GGTGA 3 cut(s) 34, 347, 1663
Hpy166II GTNNAC 4 cut(s) 155, 361, 1046, 1602
Hpy188III TCNNGA 8 cut(s) 86, 652, 660, 1254, 1288, 1684, 1729, 1810
Hpy8I GTNNAC 4 cut(s) 155, 361, 1046, 1602
Hpy99I CGWCG 4 cut(s) 266, 653, 1676, 1763
HpyAV CCTTC 6 cut(s) 55, 102, 455, 811, 1412, 1653
HpyCH4III ACNGT 6 cut(s) 130, 1204, 1344, 1561, 1625, 1859
HpyCH4IV ACGT 5 cut(s) 219, 679, 1248, 1455, 1866
HpyCH4V TGCA 7 cut(s) 168, 782, 1306, 1373, 1388, 1505, 1827
HpyF10VI GCNNNNNNNGC 9 cut(s) 264, 273, 298, 711, 740, 936, 1360, 1487, 1522
HpyF3I CTNAG 5 cut(s) 202, 272, 693, 1746, 1884
HpySE526I ACGT 5 cut(s) 219, 679, 1248, 1455, 1866
Hsp92II CATG 3 cut(s) 168, 1291, 1732
HspAI GCGC 3 cut(s) 26, 193, 1523
Ksp22I TGATCA 1 cut(s) 963
Kzo9I GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
LmnI GCTCC 6 cut(s) 754, 772, 851, 1299, 1789, 1803
Lsp1109I GCAGC 7 cut(s) 17, 30, 40, 718, 721, 819, 1372
LweI GCATC 2 cut(s) 1114, 1164
MabI ACCWGGT 1 cut(s) 1272
MaeI CTAG 2 cut(s) 764, 1241
MaeII ACGT 5 cut(s) 219, 679, 1248, 1455, 1866
MaeIII GTNAC 3 cut(s) 335, 630, 680
MalI GATC 7 cut(s) 99, 311, 965, 1189, 1196, 1440, 1889
MboI GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
MflI RGATCY 2 cut(s) 309, 1438
MhlI GDGCHC 3 cut(s) 278, 303, 856
MlyI GAGTC 2 cut(s) 72, 1711
MmeI TCCRAC 4 cut(s) 776, 1326, 1543, 1695
MroXI GAANNNNTTC 1 cut(s) 1401
MseI TTAA 2 cut(s) 897, 1710
MspA1I CMGCKG 1 cut(s) 731
MssI GTTTAAAC 1 cut(s) 898
MvaI CCWGG 6 cut(s) 515, 771, 992, 1071, 1274, 1802
MvnI CGCG 2 cut(s) 26, 1523
MwoI GCNNNNNNNGC 9 cut(s) 264, 273, 298, 711, 740, 936, 1360, 1487, 1522
NciI CCSGG 5 cut(s) 177, 278, 424, 481, 874
NdeII GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
NheI GCTAGC 1 cut(s) 1240
NlaIII CATG 3 cut(s) 168, 1291, 1732
NlaIV GGNNCC 3 cut(s) 664, 1220, 1301
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 335
NspV TTCGAA 1 cut(s) 1544
PagI TCATGA 2 cut(s) 1287, 1728
PceI AGGCCT 1 cut(s) 1852
PcsI WCGNNNNNNNCGW 6 cut(s) 93, 577, 609, 618, 635, 1461
PdmI GAANNNNTTC 1 cut(s) 1401
PfeI GAWTC 4 cut(s) 228, 379, 1225, 1318
PfoI TCCNGGA 2 cut(s) 479, 990
PinAI ACCGGT 1 cut(s) 399
Ple19I CGATCG 1 cut(s) 1190
PleI GAGTC 2 cut(s) 72, 1710
PmaCI CACGTG 1 cut(s) 1249
PmeI GTTTAAAC 1 cut(s) 898
PmlI CACGTG 1 cut(s) 1249
PpsI GAGTC 2 cut(s) 72, 1710
Ppu21I YACGTR 1 cut(s) 1249
PsiI TTATAA 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 303
Psp1406I AACGTT 1 cut(s) 679
Psp6I CCWGG 6 cut(s) 513, 769, 990, 1069, 1272, 1800
PspCI CACGTG 1 cut(s) 1249
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 6 cut(s) 513, 769, 990, 1069, 1272, 1800
PspN4I GGNNCC 3 cut(s) 664, 1220, 1301
PspPI GGNCC 2 cut(s) 773, 941
PstI CTGCAG 1 cut(s) 1390
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 2 cut(s) 309, 1438
PvuI CGATCG 1 cut(s) 1190
PvuII CAGCTG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 897, 1710
Sau3AI GATC 7 cut(s) 97, 309, 963, 1187, 1194, 1438, 1887
Sau96I GGNCC 2 cut(s) 773, 941
SchI GAGTC 2 cut(s) 72, 1711
SduI GDGCHC 3 cut(s) 278, 303, 856
SexAI ACCWGGT 1 cut(s) 1272
SfaNI GCATC 2 cut(s) 1114, 1164
SfcI CTRYAG 2 cut(s) 1203, 1386
SfuI TTCGAA 1 cut(s) 1544
SinI GGWCC 1 cut(s) 773
SmlI CTYRAG 5 cut(s) 374, 497, 744, 1442, 1808
SmoI CTYRAG 5 cut(s) 374, 497, 744, 1442, 1808
SseBI AGGCCT 1 cut(s) 1852
SsiI CCGC 8 cut(s) 24, 256, 289, 927, 939, 1035, 1328, 1490
SspMI CTAG 2 cut(s) 764, 1241
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 1852
TaaI ACNGT 6 cut(s) 130, 1204, 1344, 1561, 1625, 1859
TaiI ACGT 5 cut(s) 222, 682, 1251, 1458, 1869
TaqI TCGA 9 cut(s) 87, 261, 456, 621, 651, 1255, 1464, 1544, 1758
TauI GCSGC 3 cut(s) 292, 930, 942
TfiI GAWTC 4 cut(s) 228, 379, 1225, 1318
Tru1I TTAA 2 cut(s) 897, 1710
Tru9I TTAA 2 cut(s) 897, 1710
TscAI CASTG 3 cut(s) 861, 1207, 1380
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 7 cut(s) 5, 18, 28, 731, 734, 832, 1385
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 4 cut(s) 1146, 1178, 1276, 1304
TspGWI ACGGA 2 cut(s) 1347, 1794
TspRI CASTG 3 cut(s) 861, 1207, 1380
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 2 cut(s) 484, 614
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 1401
XspI CTAG 2 cut(s) 764, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.