Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0008551

Physical Location & Seq

Genomic Coordinates Forward (+)

57379324 .. 57381357

Gene Structure 2034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00263440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGGCTCTACACGCTTCATTCTTTTCCACATCCTCCTCCTATATAAAACAAACCTTAAGCATGAACCAGTCTACCCGCTAGTCCACAATTCAAAGCCCACCCGCCGGAAAATGTTCCTTTTTCCTTGCAAACCGCAGCTCCTCCGCCTCCTCACCTTCCTCTCCCTCCTCCTCCTCATCCACGGCGGTAGGCTCAACCTCGACCCTTCAGACCTTCAAGCCCTCATCACTGTCCAAACCCACCTCGGCTTCCACACCCCAGACAGAGCCAAACCCACCAACCCATGCAACACCCCCGGCGTCTTCTGCGAGAGAAGACTCTCCAACAACACCTACGTCCTCAAAATCACTAGACTCGTCTTCAAGTCCCAACTCCTCTCCGGCTCTCTGTCTCCGGCAATCGGACAGCTCTCCGAGCTTAAAGAGCTCTCTCTTCCGGACAACAGCCTCGTTGACCGAGTCCCGCCCGAAATCGTTGACTGCAAGAAGCTGGAGATCCTCAACCTCGGAAACAACCAGTTTTCGGGTGAAGTTCCTTCTCAGCTCTCTTCTCTAATAAGGCTTCGAATACTCGACCTCTCCTCCAACAAGTTCTCCGGCAACTTGAGCTTCTTGAAGCATTTTCCCAACTTGGAAAATCTCTCCATTGCCGACAATCTCTTCTCCGGGAAAATCCCAGCTTCGGTTCGTTCTTTTCGCAATCTCAGGTTCTTCAACTTCGCCGGAAATGAGTTCCTGGAAGGCTCGGCGCCTAAGTTGAAAAGGGTCGAGGTTTCAGCTTCTTCCGAGGTTCCAAGGCGTTTCATTTTTGCTGAAACATCAAACTCCACCACCGCAGCAAAAACCAATAATAGTAATTCAATCGCTCAAGGACCCTCCGGTTCTAACGCTCAAGCTCCAGGTCCATCTCCAGCACCGAAGAAGAAGAAGAAGAGCAAGAAGAAGAAACTCGGAGGCTGGCTGCTCGGGTTCTTCGCCGGAGCGCTGGCCGGGAGCATATCCGGGTTCATCTTCTCTCTGCTGTTCAAGCTGCTCTTGGCTGTGGTTAGAGGAGGTGGAGGCAAGGACTCTGGTCCGGCCATTTTCAGTTCTCTGATTAAGAGAAAAGAGGACTTGGCTTTTCTGGAGAAAGAAGACGGGCTTGCTTCGTTGGAACTCATCGGAAGTGGAGGATGTGGACAGGTTTACAAATCTGAGTTGCCAGGAAGCAACGGGAAGATAATCGCTATTAAGAAGATTATTCAACCTCCTAAGGAAGCGGCAGAGCTGACGGACGAAGACAGCAAGCAGATGCATAAGAAGATGCGTCAAATTCGGTCGGAGATCAATACCGTGGGACAAATTCGACACCGGAATCTGCTTCCTCTGTTGGCACATGTGTCCCGGCCGGACTGTCATCTGCTGGTGTATGAGTACATGAAAAATGGGAGCTTACAAGACATGTTGATTGAGGTTTCTGAAGGGAGAAAGGAACTGGATTGGTTAACAAGGCACAGGATTGCAATAGGAATCGCTTCAGGGCTTGAATATCTCCATGTGCACCATACACCTCACATCATTCACAGGGATCTGAAGCCGGCAAACGTCCTGCTTGATGACGATATGGAAGCTCGAATTGCAGATTTTGGGCTTGCGAAAGCAATGCCTGACTACCAGACGCATATCACAACTTCAAATGTGGCAGGAACGGTTGGTTACATTGCACCGGAGTATCATCAGACACTCAAGTTCACAGACAAGTGTGATATATACAGCTTCGGGGTATTGCTCGGGGTACTGATCATAGGAAAGCTACCGTCTGATGAATTTTTCCAGACCACCAGCGAGATGAGTCTGGTTAAGTGGATGAAGAATGTGCTGACGTCGGAGGATCCTAAGCAGGCAATCGACTCAAAATTGATGGGAAATGGATTTGAGGAGCAAATGCTCTTGGTTCTAAAGATAGCATGCTTTTGCACAGTGGATAACCCGAAAGAGAGACCTAATAGTAAGGATGTTAGGTGCATGTTGGATCAAATCAAGCACCAAGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004713 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010941 GO:0010942 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018108 GO:0018193 GO:0018212 GO:0019538 GO:0031347 GO:0031349 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_4 LRR_8 Pkinase

Protein Analysis

677

Amino Acids

75.15

Weight (kDa)

9.39

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_At5g56370	PF24758	138 - 260	4.3e-06	FBD-associated F-box protein At5g56370, LRR repeats
LRR_8	PF13855	139 - 198	8.2e-07	Leucine rich repeat
Pkinase	PF00069	384 - 665	3.9e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	384 - 624	5.9e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31880
fragaria_vesca	FvH4_5g06700
malus_domestica	MD14G1167300.v1.1 MD14G1167400.v1.1
prunus_persica	Prupe.5G161700_v2.0.a1
pyrus_communis	pycom06g14450 pycom14g13960 pycom14g13970
rosa_chinensis	RchiOBHm_Chr7g0191951 RchiOBHm_Chr7g0191971
rosa_laevigata	RLG00000004413 RLG00000004415
rosa_roxburghii	Rroxscaffold_3G00263400 Rroxscaffold_3G00263440
rosa_rugosa	Rorug06G0511800 Rorug06G0511900
rosa_samantha	Rh7AG122200 Rh7AG122300 Rh7BG123900 Rh7CG126400 Rh7CG126600
rosa_wichuraiana	Rw7G010400 Rw7G010410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1865
AccB1I	GGYRCC	1 cut(s)	748
AccI	GTMKAC	1 cut(s)	72
AccIII	TCCGGA	1 cut(s)	436
AciI	CCGC	8 cut(s)	77, 103, 134, 145, 186, 464, 834, 1259
AclWI	GGATC	5 cut(s)	490, 1575, 1865, 1878, 2019
AcoI	YGGCCR	3 cut(s)	987, 1077, 1385
AcsI	RAATTY	3 cut(s)	1311, 1341, 1805
AcuI	CTGAAG	4 cut(s)	192, 1479, 1500, 1592
AcyI	GRCGYC	3 cut(s)	300, 749, 1862
AfaI	GTAC	2 cut(s)	1415, 1776
AfeI	AGCGCT	1 cut(s)	984
AfiI	CCNNNNNNNGG	4 cut(s)	105, 401, 523, 682
AflII	CTTAAG	1 cut(s)	56
AflIII	ACRYGT	2 cut(s)	1375, 1440
AjnI	CCWGG	3 cut(s)	735, 898, 1201
Alw21I	GWGCWC	2 cut(s)	429, 1542
Alw26I	GTCTC	2 cut(s)	396, 1972
Alw44I	GTGCAC	1 cut(s)	1538
AlwI	GGATC	5 cut(s)	490, 1575, 1865, 1878, 2019
Ama87I	CYCGRG	2 cut(s)	965, 1769
Aor13HI	TCCGGA	1 cut(s)	436
Aor51HI	AGCGCT	1 cut(s)	984
AoxI	GGCC	3 cut(s)	987, 1077, 1385
ApaLI	GTGCAC	1 cut(s)	1538
ApeKI	GCWGC	4 cut(s)	136, 836, 961, 1030
ApoI	RAATTY	3 cut(s)	1311, 1341, 1805
Asp700I	GAANNNNTTC	2 cut(s)	113, 1513
AspLEI	GCGC	2 cut(s)	751, 985
AspS9I	GGNCC	3 cut(s)	872, 902, 1073
AsuC2I	CCSGG	5 cut(s)	297, 667, 991, 1003, 1384
AsuHPI	GGTGA	2 cut(s)	145, 539
AsuII	TTCGAA	1 cut(s)	565
AvaI	CYCGRG	2 cut(s)	965, 1769
AvaII	GGWCC	3 cut(s)	872, 902, 1073
AxyI	CCTNAGG	1 cut(s)	1251
BaeGI	GKGCMC	1 cut(s)	1542
BaeI	ACNNNNGTAYC	2 cut(s)	1694, 1727
BamHI	GGATCC	1 cut(s)	1870
BanI	GGYRCC	1 cut(s)	748
BanII	GRGCYC	2 cut(s)	8, 429
BbsI	GAAGAC	5 cut(s)	295, 322, 352, 1140, 1284
Bbv12I	GWGCWC	2 cut(s)	429, 1542
BbvI	GCAGC	4 cut(s)	148, 848, 948, 1017
BccI	CCATC	2 cut(s)	913, 1894
BceAI	ACGGC	1 cut(s)	199
BcgI	CGANNNNNNTGC	2 cut(s)	1624, 1658
BciT130I	CCWGG	3 cut(s)	737, 900, 1203
BclI	TGATCA	1 cut(s)	1779
BcnI	CCSGG	5 cut(s)	297, 667, 991, 1003, 1384
BcoDI	GTCTC	2 cut(s)	396, 1972
BfaI	CTAG	2 cut(s)	80, 351
BfoI	RGCGCY	2 cut(s)	752, 986
BfrI	CTTAAG	1 cut(s)	56
BisI	GCNGC	5 cut(s)	137, 837, 962, 1031, 1260
BlsI	GCNGC	5 cut(s)	138, 838, 963, 1032, 1261
Bme1390I	CCNGG	8 cut(s)	297, 667, 737, 900, 991, 1003, 1203, 1384
Bme18I	GGWCC	3 cut(s)	872, 902, 1073
BmeT110I	CYCGRG	2 cut(s)	965, 1769
BmgT120I	GGNCC	3 cut(s)	872, 902, 1073
BmiI	GGNNCC	4 cut(s)	750, 792, 874, 1872
BmrFI	CCNGG	8 cut(s)	297, 667, 737, 900, 991, 1003, 1203, 1384
BmsI	GCATC	2 cut(s)	1281, 1293
BoxI	GACNNNNGTC	1 cut(s)	1071
BpiI	GAAGAC	5 cut(s)	295, 322, 352, 1140, 1284
BpmI	CTGGAG	4 cut(s)	512, 882, 894, 1145
Bpu10I	CCTNAGC	1 cut(s)	1875
Bpu14I	TTCGAA	1 cut(s)	565
BpuEI	CTTGAG	4 cut(s)	625, 852, 876, 1709
BpuMI	CCSGG	5 cut(s)	297, 667, 991, 1003, 1384
BsaHI	GRCGYC	3 cut(s)	300, 749, 1862
BsaI	GGTCTC	1 cut(s)	1972
BsaJI	CCNNGG	7 cut(s)	181, 244, 295, 505, 786, 794, 1332
BsaWI	WCCGGW	4 cut(s)	436, 878, 1350, 1705
BsaXI	ACNNNNNCTCC	6 cut(s)	123, 153, 566, 578, 596, 608
Bsc4I	CCNNNNNNNGG	4 cut(s)	105, 401, 523, 682
Bse118I	RCCGGY	1 cut(s)	1576
Bse1I	ACTGG	3 cut(s)	68, 517, 1479
Bse21I	CCTNAGG	1 cut(s)	1251
Bse3DI	GCAATG	3 cut(s)	645, 1647, 1698
BseAI	TCCGGA	1 cut(s)	436
BseBI	CCWGG	3 cut(s)	737, 900, 1203
BseDI	CCNNGG	7 cut(s)	181, 244, 295, 505, 786, 794, 1332
BseGI	GGATG	5 cut(s)	30, 177, 1178, 1851, 1999
BseLI	CCNNNNNNNGG	4 cut(s)	105, 401, 523, 682
BseMI	GCAATG	3 cut(s)	645, 1647, 1698
BseMII	CTCAG	3 cut(s)	554, 718, 1185
BseNI	ACTGG	3 cut(s)	68, 517, 1479
BseSI	GKGCMC	1 cut(s)	1542
BseX3I	CGGCCG	1 cut(s)	1385
BseXI	GCAGC	4 cut(s)	148, 848, 948, 1017
BseYI	CCCAGC	1 cut(s)	676
Bsh1285I	CGRYCG	2 cut(s)	1319, 1388
BshFI	GGCC	3 cut(s)	989, 1079, 1387
BshNI	GGYRCC	1 cut(s)	748
BsiEI	CGRYCG	2 cut(s)	1319, 1388
BsiHKAI	GWGCWC	2 cut(s)	429, 1542
BsiHKCI	CYCGRG	2 cut(s)	965, 1769
BslFI	GGGAC	4 cut(s)	352, 446, 1350, 1366
BslI	CCNNNNNNNGG	4 cut(s)	105, 401, 523, 682
BsmAI	GTCTC	2 cut(s)	396, 1972
BsmFI	GGGAC	4 cut(s)	352, 446, 1350, 1366
BsnI	GGCC	3 cut(s)	989, 1079, 1387
Bso31I	GGTCTC	1 cut(s)	1972
BsoBI	CYCGRG	2 cut(s)	965, 1769
Bsp119I	TTCGAA	1 cut(s)	565
Bsp1286I	GDGCHC	3 cut(s)	8, 429, 1542
Bsp13I	TCCGGA	1 cut(s)	436
Bsp143I	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
BspACI	CCGC	8 cut(s)	77, 103, 134, 145, 186, 464, 834, 1259
BspANI	GGCC	3 cut(s)	989, 1079, 1387
BspCNI	CTCAG	3 cut(s)	553, 717, 1186
BspEI	TCCGGA	1 cut(s)	436
BspLI	GGNNCC	4 cut(s)	750, 792, 874, 1872
BspPI	GGATC	5 cut(s)	490, 1575, 1865, 1878, 2019
BspQI	GCTCTTC	1 cut(s)	926
BspT104I	TTCGAA	1 cut(s)	565
BspT107I	GGYRCC	1 cut(s)	748
BspTI	CTTAAG	1 cut(s)	56
BspTNI	GGTCTC	1 cut(s)	1972
BsrDI	GCAATG	3 cut(s)	645, 1647, 1698
BsrFI	RCCGGY	1 cut(s)	1576
BsrI	ACTGG	3 cut(s)	68, 517, 1479
BssAI	RCCGGY	1 cut(s)	1576
BssECI	CCNNGG	7 cut(s)	181, 244, 295, 505, 786, 794, 1332
BssMI	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
BssNI	GRCGYC	3 cut(s)	300, 749, 1862
BssT1I	CCWWGG	1 cut(s)	794
Bst2UI	CCWGG	3 cut(s)	737, 900, 1203
Bst4CI	ACNGT	6 cut(s)	232, 1333, 1394, 1690, 1797, 1960
Bst6I	CTCTTC	4 cut(s)	438, 553, 665, 926
BstACI	GRCGYC	3 cut(s)	300, 749, 1862
BstAFI	CTTAAG	1 cut(s)	56
BstBI	TTCGAA	1 cut(s)	565
BstC8I	GCNNGC	8 cut(s)	959, 987, 1143, 1286, 1578, 1632, 1881, 1948
BstDEI	CTNAG	6 cut(s)	540, 704, 753, 1194, 1251, 1875
BstDSI	CCRYGG	2 cut(s)	181, 1332
BstF5I	GGATG	5 cut(s)	30, 177, 1178, 1851, 1999
BstH2I	RGCGCY	2 cut(s)	752, 986
BstHHI	GCGC	2 cut(s)	751, 985
BstKTI	GATC	6 cut(s)	498, 1326, 1570, 1782, 1873, 2014
BstMAI	GTCTC	2 cut(s)	396, 1972
BstMBI	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
BstMCI	CGRYCG	2 cut(s)	1319, 1388
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 306, 415, 424, 606, 1027, 1616
BstNI	CCWGG	3 cut(s)	737, 900, 1203
BstNSI	RCATGY	4 cut(s)	1379, 1444, 1950, 2008
BstPAI	GACNNNNGTC	1 cut(s)	1071
BstSCI	CCNGG	8 cut(s)	295, 665, 735, 898, 989, 1001, 1201, 1382
BstSLI	GKGCMC	1 cut(s)	1542
BstV1I	GCAGC	4 cut(s)	148, 848, 948, 1017
BstV2I	GAAGAC	5 cut(s)	295, 322, 352, 1140, 1284
BstX2I	RGATCY	3 cut(s)	495, 1567, 1870
BstYI	RGATCY	3 cut(s)	495, 1567, 1870
BstZI	CGGCCG	1 cut(s)	1385
Bsu36I	CCTNAGG	1 cut(s)	1251
BsuRI	GGCC	3 cut(s)	989, 1079, 1387
BtgI	CCRYGG	2 cut(s)	181, 1332
BtsCI	GGATG	5 cut(s)	30, 177, 1178, 1851, 1999
BtsIMutI	CAGTG	2 cut(s)	228, 1965
Cac8I	GCNNGC	8 cut(s)	959, 987, 1143, 1286, 1578, 1632, 1881, 1948
CfoI	GCGC	2 cut(s)	751, 985
Cfr10I	RCCGGY	1 cut(s)	1576
Cfr13I	GGNCC	3 cut(s)	872, 902, 1073
CseI	GACGC	3 cut(s)	289, 1295, 1666
Csp6I	GTAC	2 cut(s)	1414, 1775
CviAII	CATG	8 cut(s)	62, 285, 1376, 1417, 1441, 1535, 1947, 2005
CviQI	GTAC	2 cut(s)	1414, 1775
DdeI	CTNAG	6 cut(s)	540, 704, 753, 1194, 1251, 1875
DinI	GGCGCC	1 cut(s)	750
DpnI	GATC	6 cut(s)	497, 1325, 1569, 1781, 1872, 2013
DpnII	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
EaeI	YGGCCR	3 cut(s)	987, 1077, 1385
EagI	CGGCCG	1 cut(s)	1385
Eam1104I	CTCTTC	4 cut(s)	438, 553, 665, 926
EarI	CTCTTC	4 cut(s)	438, 553, 665, 926
EciI	GGCGGA	1 cut(s)	134
Ecl136II	GAGCTC	1 cut(s)	427
EclXI	CGGCCG	1 cut(s)	1385
Eco130I	CCWWGG	1 cut(s)	794
Eco24I	GRGCYC	2 cut(s)	8, 429
Eco31I	GGTCTC	1 cut(s)	1972
Eco47I	GGWCC	3 cut(s)	872, 902, 1073
Eco47III	AGCGCT	1 cut(s)	984
Eco52I	CGGCCG	1 cut(s)	1385
Eco53kI	GAGCTC	1 cut(s)	427
Eco57I	CTGAAG	4 cut(s)	192, 1479, 1500, 1592
Eco81I	CCTNAGG	1 cut(s)	1251
Eco88I	CYCGRG	2 cut(s)	965, 1769
EcoICRI	GAGCTC	1 cut(s)	427
EcoO109I	RGGNCCY	1 cut(s)	872
EcoRII	CCWGG	3 cut(s)	735, 898, 1201
EcoT14I	CCWWGG	1 cut(s)	794
EcoT22I	ATGCAT	1 cut(s)	1296
EcoT38I	GRGCYC	2 cut(s)	8, 429
EgeI	GGCGCC	1 cut(s)	750
EheI	GGCGCC	1 cut(s)	750
ErhI	CCWWGG	1 cut(s)	794
FaeI	CATG	8 cut(s)	65, 288, 1379, 1420, 1444, 1538, 1950, 2008
FalI	AAGNNNNNCTT	4 cut(s)	1019, 1051, 1125, 1157
FaqI	GGGAC	4 cut(s)	352, 446, 1350, 1366
FatI	CATG	8 cut(s)	61, 284, 1375, 1416, 1440, 1534, 1946, 2004
FauI	CCCGC	3 cut(s)	84, 110, 471
FbaI	TGATCA	1 cut(s)	1779
FblI	GTMKAC	1 cut(s)	72
Fnu4HI	GCNGC	5 cut(s)	137, 837, 962, 1031, 1260
FokI	GGATG	5 cut(s)	17, 164, 1185, 1858, 2006
FriOI	GRGCYC	2 cut(s)	8, 429
Fsp4HI	GCNGC	5 cut(s)	137, 837, 962, 1031, 1260
FspBI	CTAG	2 cut(s)	80, 351
GlaI	GCGC	2 cut(s)	750, 984
GluI	GCNGC	5 cut(s)	137, 837, 962, 1031, 1260
GsaI	CCCAGC	1 cut(s)	680
GsuI	CTGGAG	4 cut(s)	512, 882, 894, 1145
HaeII	RGCGCY	2 cut(s)	752, 986
HaeIII	GGCC	3 cut(s)	989, 1079, 1387
HgaI	GACGC	3 cut(s)	289, 1295, 1666
HhaI	GCGC	2 cut(s)	751, 985
Hin1I	GRCGYC	3 cut(s)	300, 749, 1862
Hin1II	CATG	8 cut(s)	65, 288, 1379, 1420, 1444, 1538, 1950, 2008
Hin6I	GCGC	2 cut(s)	749, 983
HinP1I	GCGC	2 cut(s)	749, 983
HincII	GTYRAC	3 cut(s)	454, 478, 1485
HindII	GTYRAC	3 cut(s)	454, 478, 1485
HinfI	GANTC	8 cut(s)	318, 354, 459, 1067, 1354, 1509, 1831, 1889
HpaI	GTTAAC	1 cut(s)	1485
HphI	GGTGA	2 cut(s)	145, 539
Hpy166II	GTNNAC	9 cut(s)	73, 85, 454, 478, 1178, 1186, 1485, 1540, 1731
Hpy188III	TCNNGA	4 cut(s)	437, 613, 1124, 1813
Hpy8I	GTNNAC	9 cut(s)	73, 85, 454, 478, 1178, 1186, 1485, 1540, 1731
Hpy99I	CGWCG	1 cut(s)	1867
HpyAV	CCTTC	6 cut(s)	166, 216, 224, 546, 734, 1454
HpyCH4III	ACNGT	6 cut(s)	232, 1333, 1394, 1690, 1797, 1960
HpyCH4IV	ACGT	3 cut(s)	336, 1584, 1862
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 306, 415, 424, 606, 1027, 1616
HpyF3I	CTNAG	6 cut(s)	540, 704, 753, 1194, 1251, 1875
HpySE526I	ACGT	3 cut(s)	336, 1584, 1862
Hsp92I	GRCGYC	3 cut(s)	300, 749, 1862
Hsp92II	CATG	8 cut(s)	65, 288, 1379, 1420, 1444, 1538, 1950, 2008
HspAI	GCGC	2 cut(s)	749, 983
KasI	GGCGCC	1 cut(s)	748
Kpn2I	TCCGGA	1 cut(s)	436
KroI	GCCGGC	1 cut(s)	1576
KroNI	GCCGGC	1 cut(s)	1578
Ksp22I	TGATCA	1 cut(s)	1779
KspAI	GTTAAC	1 cut(s)	1485
Kzo9I	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
LguI	GCTCTTC	1 cut(s)	926
LmnI	GCTCC	6 cut(s)	144, 901, 980, 993, 1428, 1918
Lsp1109I	GCAGC	4 cut(s)	148, 848, 948, 1017
LweI	GCATC	2 cut(s)	1281, 1293
MaeI	CTAG	2 cut(s)	80, 351
MaeII	ACGT	3 cut(s)	336, 1584, 1862
MaeIII	GTNAC	1 cut(s)	1694
MalI	GATC	6 cut(s)	497, 1325, 1569, 1781, 1872, 2013
MboI	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
MflI	RGATCY	3 cut(s)	495, 1567, 1870
MhlI	GDGCHC	3 cut(s)	8, 429, 1542
MluCI	AATT	7 cut(s)	88, 856, 1311, 1341, 1614, 1805, 1895
Mly113I	GGCGCC	1 cut(s)	749
MlyI	GAGTC	6 cut(s)	312, 348, 468, 1061, 1840, 1883
MmeI	TCCRAC	6 cut(s)	348, 609, 1131, 1299, 1845, 1989
Mph1103I	ATGCAT	1 cut(s)	1296
MroI	TCCGGA	1 cut(s)	436
MroNI	GCCGGC	1 cut(s)	1576
MroXI	GAANNNNTTC	2 cut(s)	113, 1513
MseI	TTAA	6 cut(s)	57, 420, 1098, 1230, 1484, 1839
MspCI	CTTAAG	1 cut(s)	56
MspR9I	CCNGG	8 cut(s)	297, 667, 737, 900, 991, 1003, 1203, 1384
MvaI	CCWGG	3 cut(s)	737, 900, 1203
MwoI	GCNNNNNNNGC	7 cut(s)	12, 306, 415, 424, 606, 1027, 1616
NaeI	GCCGGC	1 cut(s)	1578
NarI	GGCGCC	1 cut(s)	749
NciI	CCSGG	5 cut(s)	297, 667, 991, 1003, 1384
NdeII	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
NgoMIV	GCCGGC	1 cut(s)	1576
NlaIII	CATG	8 cut(s)	65, 288, 1379, 1420, 1444, 1538, 1950, 2008
NlaIV	GGNNCC	4 cut(s)	750, 792, 874, 1872
NmeAIII	GCCGAG	2 cut(s)	225, 725
NsiI	ATGCAT	1 cut(s)	1296
NspI	RCATGY	4 cut(s)	1379, 1444, 1950, 2008
NspV	TTCGAA	1 cut(s)	565
PaeI	GCATGC	1 cut(s)	1950
PciI	ACATGT	2 cut(s)	1375, 1440
PciSI	GCTCTTC	1 cut(s)	926
PdiI	GCCGGC	1 cut(s)	1578
PdmI	GAANNNNTTC	2 cut(s)	113, 1513
PfeI	GAWTC	2 cut(s)	1354, 1509
PflFI	GACNNNGTC	1 cut(s)	458
PfoI	TCCNGGA	2 cut(s)	665, 735
PkrI	GCNGC	5 cut(s)	138, 838, 963, 1032, 1261
PleI	GAGTC	6 cut(s)	312, 348, 467, 1061, 1839, 1883
PluTI	GGCGCC	1 cut(s)	752
PpsI	GAGTC	6 cut(s)	312, 348, 467, 1061, 1839, 1883
PpuMI	RGGWCCY	1 cut(s)	872
PscI	ACATGT	2 cut(s)	1375, 1440
PshAI	GACNNNNGTC	1 cut(s)	1071
Psp124BI	GAGCTC	1 cut(s)	429
Psp5II	RGGWCCY	1 cut(s)	872
Psp6I	CCWGG	3 cut(s)	735, 898, 1201
PspFI	CCCAGC	1 cut(s)	676
PspGI	CCWGG	3 cut(s)	735, 898, 1201
PspN4I	GGNNCC	4 cut(s)	750, 792, 874, 1872
PspPI	GGNCC	3 cut(s)	872, 902, 1073
PspPPI	RGGWCCY	1 cut(s)	872
PsuI	RGATCY	3 cut(s)	495, 1567, 1870
PsyI	GACNNNGTC	1 cut(s)	458
RsaI	GTAC	2 cut(s)	1415, 1776
RsaNI	GTAC	2 cut(s)	1414, 1775
SacI	GAGCTC	1 cut(s)	429
SapI	GCTCTTC	1 cut(s)	926
SaqAI	TTAA	6 cut(s)	57, 420, 1098, 1230, 1484, 1839
SatI	GCNGC	5 cut(s)	137, 837, 962, 1031, 1260
Sau3AI	GATC	6 cut(s)	495, 1323, 1567, 1779, 1870, 2011
Sau96I	GGNCC	3 cut(s)	872, 902, 1073
SchI	GAGTC	6 cut(s)	312, 348, 468, 1061, 1840, 1883
ScrFI	CCNGG	8 cut(s)	297, 667, 737, 900, 991, 1003, 1203, 1384
SduI	GDGCHC	3 cut(s)	8, 429, 1542
SfaNI	GCATC	2 cut(s)	1281, 1293
SfoI	GGCGCC	1 cut(s)	750
SfuI	TTCGAA	1 cut(s)	565
SinI	GGWCC	3 cut(s)	872, 902, 1073
SmlI	CTYRAG	5 cut(s)	56, 604, 867, 891, 1724
SmoI	CTYRAG	5 cut(s)	56, 604, 867, 891, 1724
SphI	GCATGC	1 cut(s)	1950
Sse9I	AATT	7 cut(s)	88, 856, 1311, 1341, 1614, 1805, 1895
SsiI	CCGC	8 cut(s)	77, 103, 134, 145, 186, 464, 834, 1259
SspDI	GGCGCC	1 cut(s)	748
SspMI	CTAG	2 cut(s)	80, 351
SstI	GAGCTC	1 cut(s)	429
StyD4I	CCNGG	8 cut(s)	295, 665, 735, 898, 989, 1001, 1201, 1382
StyI	CCWWGG	1 cut(s)	794
TaaI	ACNGT	6 cut(s)	232, 1333, 1394, 1690, 1797, 1960
TaiI	ACGT	3 cut(s)	339, 1587, 1865
TaqI	TCGA	7 cut(s)	201, 565, 573, 768, 1345, 1612, 1887
TaqII	GACCGA	2 cut(s)	471, 1305
TasI	AATT	7 cut(s)	88, 856, 1311, 1341, 1614, 1805, 1895
TatI	WGTACW	1 cut(s)	1413
TauI	GCSGC	1 cut(s)	1262
TfiI	GAWTC	2 cut(s)	1354, 1509
Tru1I	TTAA	6 cut(s)	57, 420, 1098, 1230, 1484, 1839
Tru9I	TTAA	6 cut(s)	57, 420, 1098, 1230, 1484, 1839
TscAI	CASTG	2 cut(s)	235, 1965
TseI	GCWGC	4 cut(s)	136, 836, 961, 1030
TspDTI	ATGAA	7 cut(s)	7, 78, 793, 997, 1433, 1818, 1862
TspGWI	ACGGA	1 cut(s)	1286
TspRI	CASTG	2 cut(s)	235, 1965
Tth111I	GACNNNGTC	1 cut(s)	458
Vha464I	CTTAAG	1 cut(s)	56
VneI	GTGCAC	1 cut(s)	1538
VpaK11BI	GGWCC	3 cut(s)	872, 902, 1073
XapI	RAATTY	3 cut(s)	1311, 1341, 1805
XceI	RCATGY	4 cut(s)	1379, 1444, 1950, 2008
XmiI	GTMKAC	1 cut(s)	72
XmnI	GAANNNNTTC	2 cut(s)	113, 1513
XspI	CTAG	2 cut(s)	80, 351
ZraI	GACGTC	1 cut(s)	1863
Zsp2I	ATGCAT	1 cut(s)	1296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.