pycom14g15400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012810
Physical Location & Seq
Forward (+)
18038788 .. 18040313
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGGGAAATTGTCAAGCGATTGATGCAGCAACACTGGTAGTACAACACCCAAGTGGGAAAGAAGAGAAGTTCTATTGGGCAGTGAGTGCAAGTGAGATTATGAAGATGAACCCTGGCCACTATGTTGCTCTTCTCATCTCCACCACCTTGTGTCCCTCCAATCCCACCACCGACAATGGCGGTGACAGGAAAGACAAGAACTCGAATACGGACGACAACAAGAACACCGCTTCTTCGCTTCGCGTGACGCGCATAAAGCTCCTCAGGCCAACCGATTCTCTCGTTCTTGGCAAAGTTTACAGACTCATCACCACTCAAGAAGTGATGAAGGGATTGTGGGCAAAGAAACAAACGAAGGTGAAGAGAAATCATAATCATCAGGTGGAAGGAGAGCAGAAGCCACAAGTGGAGAGGGTGATGAGAGAGAAGCAAGTTCTGACATCAGCCAGAAGATCTGAGACTTCTGAGCTGGAGAAGGACATCCAGGTGAATAATAAACATGAGAGAGGAGGACACCGACCAAGAACAGCAACAACATCAAGAACGTGGCAGCCATCATTACACAGCATCTCAGAGGCTGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

196

Amino Acids

21.92

Weight (kDa)

9.75

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 192 3e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 243, 250
AciI CCGC 2 cut(s) 180, 228
AcoI YGGCCR 1 cut(s) 115
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 1 cut(s) 42
AjnI CCWGG 2 cut(s) 112, 483
AleI CACNNNNGTG 1 cut(s) 51
AluBI AGCT 2 cut(s) 259, 469
AluI AGCT 2 cut(s) 259, 469
Alw26I GTCTC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 2 cut(s) 115, 266
ApeKI GCWGC 3 cut(s) 26, 550, 578
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 5 cut(s) 194, 301, 370, 427, 499
AxyI CCTNAGG 1 cut(s) 263
BalI TGGCCA 1 cut(s) 117
BbvI GCAGC 3 cut(s) 38, 562, 565
BccI CCATC 1 cut(s) 562
BciT130I CCWGG 2 cut(s) 114, 485
BcoDI GTCTC 1 cut(s) 452
BglII AGATCT 1 cut(s) 452
BisI GCNGC 3 cut(s) 27, 551, 579
BlsI GCNGC 3 cut(s) 28, 552, 580
Bme1390I CCNGG 2 cut(s) 114, 485
BmrFI CCNGG 2 cut(s) 114, 485
BmsI GCATC 2 cut(s) 13, 576
BpmI CTGGAG 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 39
Bse21I CCTNAGG 1 cut(s) 263
BseBI CCWGG 2 cut(s) 114, 485
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 480
BseMII CTCAG 4 cut(s) 277, 447, 456, 585
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 2 cut(s) 251, 522
BseXI GCAGC 3 cut(s) 38, 562, 565
Bsh1236I CGCG 2 cut(s) 243, 250
BshFI GGCC 2 cut(s) 117, 268
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 117, 268
Bsp143I GATC 1 cut(s) 452
BspACI CCGC 2 cut(s) 180, 228
BspANI GGCC 2 cut(s) 117, 268
BspCNI CTCAG 4 cut(s) 276, 448, 457, 584
BspFNI CGCG 2 cut(s) 243, 250
BspQI GCTCTTC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 1 cut(s) 452
Bst2UI CCWGG 2 cut(s) 114, 485
Bst6I CTCTTC 3 cut(s) 57, 135, 356
BstAPI GCANNNNNTGC 1 cut(s) 86
BstDEI CTNAG 4 cut(s) 263, 456, 465, 571
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 2 cut(s) 243, 250
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 1 cut(s) 455
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 452
BstMWI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
BstNI CCWGG 2 cut(s) 114, 485
BstSCI CCNGG 2 cut(s) 112, 483
BstUI CGCG 2 cut(s) 243, 250
BstV1I GCAGC 3 cut(s) 38, 562, 565
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
Bsu36I CCTNAGG 1 cut(s) 263
BsuRI GGCC 2 cut(s) 117, 268
BtsCI GGATG 1 cut(s) 480
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 32, 87
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 1 cut(s) 252
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 500
CviJI RGCY 8 cut(s) 117, 259, 268, 400, 446, 469, 553, 578
CviKI_1 RGCY 8 cut(s) 117, 259, 268, 400, 446, 469, 553, 578
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 4 cut(s) 263, 456, 465, 571
DpnI GATC 1 cut(s) 454
DpnII GATC 1 cut(s) 452
DraIII CACNNNGTG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 57, 135, 356
EarI CTCTTC 3 cut(s) 57, 135, 356
Eco81I CCTNAGG 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 112, 483
FaeI CATG 1 cut(s) 503
FaiI YATR 5 cut(s) 101, 123, 254, 372, 501
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 27, 551, 579
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 3 cut(s) 27, 551, 579
GlaI GCGC 1 cut(s) 251
GluI GCNGC 3 cut(s) 27, 551, 579
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 2 cut(s) 117, 268
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 2 cut(s) 275, 303
HphI GGTGA 5 cut(s) 194, 301, 370, 427, 499
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 4 cut(s) 438, 457, 466, 574
Hpy188III TCNNGA 2 cut(s) 317, 540
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 322, 349, 380, 469
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 3 cut(s) 26, 89, 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
HpyF3I CTNAG 4 cut(s) 263, 456, 465, 571
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 1 cut(s) 250
Kzo9I GATC 1 cut(s) 452
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 3 cut(s) 38, 562, 565
LweI GCATC 2 cut(s) 13, 576
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 2 cut(s) 182, 244
MalI GATC 1 cut(s) 454
MboI GATC 1 cut(s) 452
MboII GAAGA 6 cut(s) 74, 115, 122, 225, 373, 462
MflI RGATCY 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 6 cut(s) 166, 272, 405, 500, 503, 568
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 2 cut(s) 51, 485
Msp20I TGGCCA 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 114, 485
MvaI CCWGG 2 cut(s) 114, 485
MvnI CGCG 2 cut(s) 243, 250
MwoI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
NdeII GATC 1 cut(s) 452
NlaIII CATG 1 cut(s) 503
NmuCI GTSAC 2 cut(s) 182, 244
OliI CACNNNNGTG 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 3 cut(s) 28, 552, 580
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 112, 483
PspGI CCWGG 2 cut(s) 112, 483
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 452
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 2 cut(s) 51, 485
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 3 cut(s) 27, 551, 579
Sau3AI GATC 1 cut(s) 452
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 2 cut(s) 114, 485
SetI ASST 7 cut(s) 149, 261, 360, 384, 471, 489, 548
SfaNI GCATC 2 cut(s) 13, 576
SmiMI CAYNNNNRTG 2 cut(s) 51, 485
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 1 cut(s) 7
SsiI CCGC 2 cut(s) 180, 228
StyD4I CCNGG 2 cut(s) 112, 483
TaiI ACGT 1 cut(s) 548
TaqI TCGA 1 cut(s) 203
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 1 cut(s) 275
TscAI CASTG 2 cut(s) 39, 87
TseFI GTSAC 2 cut(s) 182, 244
TseI GCWGC 3 cut(s) 26, 550, 578
Tsp45I GTSAC 2 cut(s) 182, 244
TspDTI ATGAA 3 cut(s) 116, 122, 341
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 2 cut(s) 39, 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.