Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0015351

Physical Location & Seq

Genomic Coordinates Reverse (-)

18152094 .. 18153620

Gene Structure 1527 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGTTTGGCTGCCGAGAAAATAAGGAGGGAAAATTACCTGCCAACATCTTCAACCGCAGCAGAATGCTCACTCTGCAACTCAAAACTGCCGCACAGAAAAACCCACAACCCAGTTTTCTAATTCCTTGTCGGAGCTTCACCACCTCACCTTCTCATCCCTCTCAAGACTCTCACTTGGCGAACCTGATTCTCAAATCCGACCCGCAAACCCTAACCCAGATTCTTCACAGCCCCCAAATCGATTGGACTTCCGATTTGGTCGACAAAACCCTGAAGAGGCTCTGGAATCATGGGCCCAAAGCCCTCCAGTTCTTCAGAATCCTCGACCACCATCCGAACTACACTCACTCTTGCTCCTCCTTCGACCACGCCGTCGATATTGCCGGCCGCCTGCGCGACTACAAGTCGCTCTGGACCCTCGTGGCCCGAATGCGGGCCCGCAGGCTCGGCCCTGGGCCGAGGACTTTCGCTATCATCACGGAGAGGTATGTGGCTGCTGGTAAGCCTGATAGAGCCGTTAAGGTGTTCCTGTCAATGCATGAACATGGTTGTCCTCAGGATTTGAATTCGTTTAATACAATTCTTGACGTGCTTTGTAAAGCTAAGCGTGTCGAAAAGGCGTATAATTTGTTTAAGGTGTTTAGGGGCAGGTTTAAGGCTGATTGTGTTAGTTACAATATAATTGCGAACGGGTGGTGTTTGATTAAGCGAACCCCGAAAGCTTTGGAGCTTTTGGGGGAGATGGTGGAGAGGGGATTGGACCCGAGTTTAACGACGTTTAACATAATGCTTAAAGGATATTTTAGAGCTGGTCAGATTAAGGAAGCTTGGGAGTTCTTTTTGCAAATGAAGAAGAGGAAGTGTGAGATTGATGTTGTTACCTACACTACTTTGGTTCATGGGTTTGGTGTTGTCGGGGAGATTAAGAAAGCTCGAAAGGTTTTTGATGAGATGGTTGGGGAAGGGGTGCTTCCTTCGGTGGCAACTTACAATGCTTTGATTCAGGTTTTGTGTAAGAAAGATAGTGTGGAAAATGCTGTTGTGGTTTTTGAGGAGATGGTGAGCAAGGGTTATGTGCCTAATGTGACGACTTATAATGTATTGATTCGAGGGTTGTGTCACTCGGGAAACATGGATAGGGCGCTGGAGTTTATGGACAGAATGAAGGGGGATGAGTGTGAGCCAAATGTTCAGACATATAATGTTGTGATTCGTTACTTTTGTGATGCCGGAGAGATTGAGAAGGCGTTGAATGTGTTTGAGAAGATGGGTTGTGGTGATTGCTTGCCTAATTTGGACACGTACAATGTTTTGATCAGTGCCATGTTTGTGAGGAAGAAACCTGAAGACCTGTTAGTGGCAGGGAAGTTGTTGATTGAGATGGTTGATCGAGGATTTTTGCCGCGAAGGTTCACCTTCAATCGCGTTCTGGATGGGCTTTTATTAACAGGTAATCAAGCTTTTGCAAAAGAGATTTTGAGATTGCAGAGCAAATGTGGTCGTCTTCCCCGTCAAGTAAAATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

509

Amino Acids

57.78

Weight (kDa)

9.36

Isoelectric Point (pI)

36.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	221 - 268	6.3e-10	PPR repeat family
PPR_1	PF12854	251 - 284	1.6e-07	PPR repeat
PPR_2	PF13041	259 - 302	2e-16	PPR repeat family
PPR	PF01535	259 - 288	2.2e-07	PPR repeat
TPR_24	PF23276	271 - 384	4.8e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	283 - 337	4.6e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	287 - 319	4.9e-09	PPR repeat
PPR_2	PF13041	291 - 331	3e-10	PPR repeat family
PPR	PF01535	293 - 323	1e-06	PPR repeat
PPR_long	PF17177	308 - 439	4.6e-15	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	322 - 354	5e-09	PPR repeat
PPR_2	PF13041	325 - 374	3e-18	PPR repeat family
PPR	PF01535	329 - 357	7e-07	PPR repeat
PPR_3	PF13812	349 - 407	4.9e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	356 - 389	1.4e-11	PPR repeat
PPR_2	PF13041	360 - 409	2.5e-16	PPR repeat family
PPR	PF01535	364 - 393	8e-08	PPR repeat
PPR_1	PF12854	392 - 423	4e-09	PPR repeat
PPR_2	PF13041	395 - 442	3.9e-14	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74900
fragaria_vesca	FvH4_5g16320
malus_domestica	MD06G1181800.v1.1 MD14G1187700.v1.1
prunus_persica	Prupe.5G180100_v2.0.a1
pyrus_communis	pycom06g16080 pycom14g15560
rosa_chinensis	RchiOBHm_Chr7g0179811
rosa_laevigata	RLG00000005322
rosa_multiflora	Rmu_sc0001754.1_g000006 Rmu_sc0004156.1_g000009
rosa_roxburghii	Rroxscaffold_3G00273450
rosa_rugosa	Rorug06G0428500
rosa_samantha	Rh7CG029100 Rh7DG028600
rosa_wichuraiana	Rw7G002250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1095
AasI	GACNNNNNNGTC	1 cut(s)	371
Acc36I	ACCTGC	2 cut(s)	46, 639
AccI	GTMKAC	1 cut(s)	261
AccII	CGCG	3 cut(s)	396, 1405, 1425
AciI	CCGC	7 cut(s)	55, 90, 203, 388, 433, 439, 1403
AcoI	YGGCCR	1 cut(s)	385
AcsI	RAATTY	1 cut(s)	565
AcuI	CTGAAG	3 cut(s)	293, 298, 1365
AfaI	GTAC	1 cut(s)	1304
AfiI	CCNNNNNNNGG	4 cut(s)	276, 432, 433, 1357
AflIII	ACRYGT	1 cut(s)	1299
AgsI	TTSAA	4 cut(s)	52, 565, 1252, 1420
AhdI	GACNNNNNGTC	1 cut(s)	403
AjiI	CACGTC	1 cut(s)	589
AjnI	CCWGG	1 cut(s)	451
AluBI	AGCT	8 cut(s)	135, 602, 722, 730, 809, 827, 932, 1460
AluI	AGCT	8 cut(s)	135, 602, 722, 730, 809, 827, 932, 1460
Ama87I	CYCGRG	2 cut(s)	763, 1123
AoxI	GGCC	6 cut(s)	293, 385, 423, 435, 448, 455
ApaI	GGGCCC	2 cut(s)	297, 439
ApeKI	GCWGC	3 cut(s)	9, 57, 494
ApoI	RAATTY	1 cut(s)	565
ArsI	GACNNNNNNTTYG	2 cut(s)	238, 270
AspLEI	GCGC	2 cut(s)	396, 1144
AspS9I	GGNCC	9 cut(s)	293, 294, 414, 424, 435, 436, 449, 455, 760
AsuHPI	GGTGA	5 cut(s)	130, 138, 1072, 1289, 1405
AvaI	CYCGRG	2 cut(s)	763, 1123
AvaII	GGWCC	2 cut(s)	414, 760
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	2 cut(s)	297, 439
BanII	GRGCYC	2 cut(s)	297, 439
BauI	CACGAG	1 cut(s)	419
BbsI	GAAGAC	2 cut(s)	1353, 1496
BbvI	GCAGC	2 cut(s)	69, 481
BccI	CCATC	7 cut(s)	339, 736, 946, 1051, 1261, 1375, 1427
BceAI	ACGGC	2 cut(s)	356, 500
BciT130I	CCWGG	1 cut(s)	453
BclI	TGATCA	1 cut(s)	1314
BfoI	RGCGCY	1 cut(s)	1145
BfuAI	ACCTGC	2 cut(s)	46, 639
BisI	GCNGC	6 cut(s)	10, 58, 90, 388, 495, 1403
BlpI	GCTNAGC	1 cut(s)	603
BlsI	GCNGC	6 cut(s)	11, 59, 91, 389, 496, 1404
Bme1390I	CCNGG	1 cut(s)	453
Bme18I	GGWCC	2 cut(s)	414, 760
BmeRI	GACNNNNNGTC	1 cut(s)	403
BmeT110I	CYCGRG	2 cut(s)	763, 1123
BmgBI	CACGTC	1 cut(s)	589
BmgT120I	GGNCC	9 cut(s)	293, 294, 414, 424, 435, 436, 449, 455, 760
BmiI	GGNNCC	4 cut(s)	295, 416, 437, 762
BmrFI	CCNGG	1 cut(s)	453
BmrI	ACTGGG	1 cut(s)	105
BmsI	GCATC	1 cut(s)	1216
BmuI	ACTGGG	1 cut(s)	105
BpiI	GAAGAC	2 cut(s)	1353, 1496
BpmI	CTGGAG	2 cut(s)	290, 1166
Bpu1102I	GCTNAGC	1 cut(s)	603
BpuEI	CTTGAG	1 cut(s)	147
Bsa29I	ATCGAT	1 cut(s)	240
BsaAI	YACGTR	1 cut(s)	1302
BsaJI	CCNNGG	3 cut(s)	451, 452, 458
BsaXI	ACNNNNNCTCC	2 cut(s)	338, 368
Bsc4I	CCNNNNNNNGG	4 cut(s)	276, 432, 433, 1357
Bse118I	RCCGGY	1 cut(s)	383
Bse1I	ACTGG	2 cut(s)	111, 307
Bse21I	CCTNAGG	1 cut(s)	555
BseBI	CCWGG	1 cut(s)	453
BseCI	ATCGAT	1 cut(s)	240
BseDI	CCNNGG	3 cut(s)	451, 452, 458
BseGI	GGATG	4 cut(s)	154, 331, 1177, 1438
BseLI	CCNNNNNNNGG	4 cut(s)	276, 432, 433, 1357
BseMII	CTCAG	1 cut(s)	569
BseNI	ACTGG	2 cut(s)	111, 307
BseRI	GAGGAG	2 cut(s)	346, 1067
BseSI	GKGCMC	2 cut(s)	297, 439
BseX3I	CGGCCG	1 cut(s)	385
BseXI	GCAGC	2 cut(s)	69, 481
Bsh1236I	CGCG	3 cut(s)	396, 1405, 1425
Bsh1285I	CGRYCG	1 cut(s)	388
BshFI	GGCC	6 cut(s)	295, 387, 425, 437, 450, 457
BshVI	ATCGAT	1 cut(s)	240
BsiEI	CGRYCG	1 cut(s)	388
BsiHKCI	CYCGRG	2 cut(s)	763, 1123
BsiSI	CCGG	2 cut(s)	384, 1230
BslI	CCNNNNNNNGG	4 cut(s)	276, 432, 433, 1357
BsmI	GAATGC	2 cut(s)	69, 435
BsnI	GGCC	6 cut(s)	295, 387, 425, 437, 450, 457
BsoBI	CYCGRG	2 cut(s)	763, 1123
Bsp120I	GGGCCC	2 cut(s)	293, 435
Bsp1286I	GDGCHC	2 cut(s)	297, 439
Bsp143I	GATC	2 cut(s)	1314, 1387
Bsp1720I	GCTNAGC	1 cut(s)	603
BspACI	CCGC	7 cut(s)	55, 90, 203, 388, 433, 439, 1403
BspANI	GGCC	6 cut(s)	295, 387, 425, 437, 450, 457
BspCNI	CTCAG	1 cut(s)	568
BspDI	ATCGAT	1 cut(s)	240
BspFNI	CGCG	3 cut(s)	396, 1405, 1425
BspLI	GGNNCC	4 cut(s)	295, 416, 437, 762
BspMI	ACCTGC	2 cut(s)	46, 639
BsrFI	RCCGGY	1 cut(s)	383
BsrI	ACTGG	2 cut(s)	111, 307
BssAI	RCCGGY	1 cut(s)	383
BssECI	CCNNGG	3 cut(s)	451, 452, 458
BssMI	GATC	2 cut(s)	1314, 1387
BssSI	CACGAG	1 cut(s)	419
Bst2BI	CACGAG	1 cut(s)	419
Bst2UI	CCWGG	1 cut(s)	453
Bst6I	CTCTTC	2 cut(s)	269, 848
BstBAI	YACGTR	1 cut(s)	1302
BstC8I	GCNNGC	6 cut(s)	385, 392, 435, 439, 443, 1286
BstDEI	CTNAG	2 cut(s)	555, 603
BstF5I	GGATG	4 cut(s)	154, 331, 1177, 1438
BstFNI	CGCG	3 cut(s)	396, 1405, 1425
BstH2I	RGCGCY	1 cut(s)	1145
BstHHI	GCGC	2 cut(s)	396, 1144
BstKTI	GATC	2 cut(s)	1317, 1390
BstMBI	GATC	2 cut(s)	1314, 1387
BstMCI	CGRYCG	1 cut(s)	388
BstMWI	GCNNNNNNNGC	3 cut(s)	73, 393, 447
BstNI	CCWGG	1 cut(s)	453
BstSCI	CCNGG	1 cut(s)	451
BstSLI	GKGCMC	2 cut(s)	297, 439
BstUI	CGCG	3 cut(s)	396, 1405, 1425
BstV1I	GCAGC	2 cut(s)	69, 481
BstV2I	GAAGAC	2 cut(s)	1353, 1496
BstZI	CGGCCG	1 cut(s)	385
Bsu15I	ATCGAT	1 cut(s)	240
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	6 cut(s)	295, 387, 425, 437, 450, 457
BsuTUI	ATCGAT	1 cut(s)	240
BtrI	CACGTC	1 cut(s)	589
BtsCI	GGATG	4 cut(s)	154, 331, 1177, 1438
BtsIMutI	CAGTG	1 cut(s)	1324
BveI	ACCTGC	2 cut(s)	46, 639
Cac8I	GCNNGC	6 cut(s)	385, 392, 435, 439, 443, 1286
CfoI	GCGC	2 cut(s)	396, 1144
Cfr10I	RCCGGY	1 cut(s)	383
Cfr13I	GGNCC	9 cut(s)	293, 294, 414, 424, 435, 436, 449, 455, 760
ClaI	ATCGAT	1 cut(s)	240
Csp6I	GTAC	1 cut(s)	1303
CviAII	CATG	6 cut(s)	290, 539, 545, 899, 1132, 1324
CviQI	GTAC	1 cut(s)	1303
DdeI	CTNAG	2 cut(s)	555, 603
DpnI	GATC	2 cut(s)	1316, 1389
DpnII	GATC	2 cut(s)	1314, 1387
DrdI	GACNNNNNNGTC	1 cut(s)	371
DriI	GACNNNNNGTC	1 cut(s)	403
DseDI	GACNNNNNNGTC	1 cut(s)	371
EaeI	YGGCCR	1 cut(s)	385
EagI	CGGCCG	1 cut(s)	385
Eam1104I	CTCTTC	2 cut(s)	269, 848
Eam1105I	GACNNNNNGTC	1 cut(s)	403
EarI	CTCTTC	2 cut(s)	269, 848
EclXI	CGGCCG	1 cut(s)	385
Eco24I	GRGCYC	2 cut(s)	297, 439
Eco47I	GGWCC	2 cut(s)	414, 760
Eco52I	CGGCCG	1 cut(s)	385
Eco57I	CTGAAG	3 cut(s)	293, 298, 1365
Eco81I	CCTNAGG	1 cut(s)	555
Eco88I	CYCGRG	2 cut(s)	763, 1123
EcoRI	GAATTC	1 cut(s)	565
EcoRII	CCWGG	1 cut(s)	451
EcoT22I	ATGCAT	1 cut(s)	540
EcoT38I	GRGCYC	2 cut(s)	297, 439
FaeI	CATG	6 cut(s)	293, 542, 548, 902, 1135, 1327
FalI	AAGNNNNNCTT	2 cut(s)	954, 986
FatI	CATG	6 cut(s)	289, 538, 544, 898, 1131, 1323
FauI	CCCGC	3 cut(s)	210, 426, 446
FbaI	TGATCA	1 cut(s)	1314
FblI	GTMKAC	1 cut(s)	261
Fnu4HI	GCNGC	6 cut(s)	10, 58, 90, 388, 495, 1403
FokI	GGATG	4 cut(s)	141, 318, 1184, 1445
FriOI	GRGCYC	2 cut(s)	297, 439
Fsp4HI	GCNGC	6 cut(s)	10, 58, 90, 388, 495, 1403
GlaI	GCGC	2 cut(s)	395, 1143
GluI	GCNGC	6 cut(s)	10, 58, 90, 388, 495, 1403
GsuI	CTGGAG	2 cut(s)	290, 1166
HaeII	RGCGCY	1 cut(s)	1145
HaeIII	GGCC	6 cut(s)	295, 387, 425, 437, 450, 457
HapII	CCGG	2 cut(s)	384, 1230
HhaI	GCGC	2 cut(s)	396, 1144
Hin1II	CATG	6 cut(s)	293, 542, 548, 902, 1135, 1327
Hin6I	GCGC	2 cut(s)	394, 1142
HinP1I	GCGC	2 cut(s)	394, 1142
HincII	GTYRAC	1 cut(s)	262
HindII	GTYRAC	1 cut(s)	262
HindIII	AAGCTT	3 cut(s)	720, 825, 1458
HinfI	GANTC	8 cut(s)	167, 187, 220, 286, 318, 1000, 1105, 1210
HpaII	CCGG	2 cut(s)	384, 1230
HphI	GGTGA	5 cut(s)	130, 138, 1072, 1289, 1405
Hpy166II	GTNNAC	2 cut(s)	262, 1413
Hpy188I	TCNGA	7 cut(s)	132, 199, 253, 317, 336, 816, 1194
Hpy188III	TCNNGA	7 cut(s)	164, 283, 412, 557, 584, 1125, 1430
Hpy8I	GTNNAC	2 cut(s)	262, 1413
Hpy99I	CGWCG	2 cut(s)	377, 778
HpyAV	CCTTC	8 cut(s)	159, 370, 956, 984, 1159, 1237, 1401, 1426
HpyCH4IV	ACGT	3 cut(s)	588, 776, 1301
HpyCH4V	TGCA	5 cut(s)	76, 538, 844, 1466, 1486
HpyF10VI	GCNNNNNNNGC	3 cut(s)	73, 393, 447
HpyF3I	CTNAG	2 cut(s)	555, 603
HpySE526I	ACGT	3 cut(s)	588, 776, 1301
Hsp92II	CATG	6 cut(s)	293, 542, 548, 902, 1135, 1327
HspAI	GCGC	2 cut(s)	394, 1142
KroI	GCCGGC	1 cut(s)	383
KroNI	GCCGGC	1 cut(s)	385
Ksp22I	TGATCA	1 cut(s)	1314
Kzo9I	GATC	2 cut(s)	1314, 1387
LmnI	GCTCC	3 cut(s)	132, 359, 727
Lsp1109I	GCAGC	2 cut(s)	69, 481
LweI	GCATC	1 cut(s)	1216
MaeII	ACGT	3 cut(s)	588, 776, 1301
MaeIII	GTNAC	5 cut(s)	671, 877, 1084, 1118, 1214
MalI	GATC	2 cut(s)	1316, 1389
MboI	GATC	2 cut(s)	1314, 1387
MhlI	GDGCHC	2 cut(s)	297, 439
MluCI	AATT	8 cut(s)	32, 120, 565, 579, 625, 681, 1291, 1520
MlyI	GAGTC	1 cut(s)	161
MmeI	TCCRAC	2 cut(s)	110, 222
Mph1103I	ATGCAT	1 cut(s)	540
MroNI	GCCGGC	1 cut(s)	383
MslI	CAYNNNNRTG	2 cut(s)	543, 1328
MspI	CCGG	2 cut(s)	384, 1230
MspR9I	CCNGG	1 cut(s)	453
Mva1269I	GAATGC	2 cut(s)	69, 435
MvaI	CCWGG	1 cut(s)	453
MvnI	CGCG	3 cut(s)	396, 1405, 1425
MwoI	GCNNNNNNNGC	3 cut(s)	73, 393, 447
NaeI	GCCGGC	1 cut(s)	385
NdeII	GATC	2 cut(s)	1314, 1387
NgoMIV	GCCGGC	1 cut(s)	383
NlaIII	CATG	6 cut(s)	293, 542, 548, 902, 1135, 1327
NlaIV	GGNNCC	4 cut(s)	295, 416, 437, 762
NmeAIII	GCCGAG	3 cut(s)	38, 426, 483
NmuCI	GTSAC	2 cut(s)	1084, 1118
NsiI	ATGCAT	1 cut(s)	540
PasI	CCCWGGG	1 cut(s)	452
PcsI	WCGNNNNNNNCGW	2 cut(s)	369, 1507
PctI	GAATGC	2 cut(s)	69, 435
PdiI	GCCGGC	1 cut(s)	385
PfeI	GAWTC	7 cut(s)	187, 220, 286, 318, 1000, 1105, 1210
PkrI	GCNGC	6 cut(s)	11, 59, 91, 389, 496, 1404
PleI	GAGTC	1 cut(s)	161
PpsI	GAGTC	1 cut(s)	161
Ppu21I	YACGTR	1 cut(s)	1302
PsiI	TTATAA	1 cut(s)	1095
Psp6I	CCWGG	1 cut(s)	451
PspGI	CCWGG	1 cut(s)	451
PspN4I	GGNNCC	4 cut(s)	295, 416, 437, 762
PspOMI	GGGCCC	2 cut(s)	293, 435
PspPI	GGNCC	9 cut(s)	293, 294, 414, 424, 435, 436, 449, 455, 760
RsaI	GTAC	1 cut(s)	1304
RsaNI	GTAC	1 cut(s)	1303
RseI	CAYNNNNRTG	2 cut(s)	543, 1328
SalI	GTCGAC	1 cut(s)	260
SatI	GCNGC	6 cut(s)	10, 58, 90, 388, 495, 1403
Sau3AI	GATC	2 cut(s)	1314, 1387
Sau96I	GGNCC	9 cut(s)	293, 294, 414, 424, 435, 436, 449, 455, 760
SchI	GAGTC	1 cut(s)	161
ScrFI	CCNGG	1 cut(s)	453
SduI	GDGCHC	2 cut(s)	297, 439
SfaNI	GCATC	1 cut(s)	1216
SinI	GGWCC	2 cut(s)	414, 760
SmiMI	CAYNNNNRTG	2 cut(s)	543, 1328
SmlI	CTYRAG	1 cut(s)	162
SmoI	CTYRAG	1 cut(s)	162
Sse9I	AATT	8 cut(s)	32, 120, 565, 579, 625, 681, 1291, 1520
SsiI	CCGC	7 cut(s)	55, 90, 203, 388, 433, 439, 1403
StyD4I	CCNGG	1 cut(s)	451
TaiI	ACGT	3 cut(s)	591, 779, 1304
TaqI	TCGA	9 cut(s)	240, 261, 324, 363, 375, 612, 934, 1108, 1390
TasI	AATT	8 cut(s)	32, 120, 565, 579, 625, 681, 1291, 1520
TauI	GCSGC	3 cut(s)	92, 390, 1405
TfiI	GAWTC	7 cut(s)	187, 220, 286, 318, 1000, 1105, 1210
TscAI	CASTG	1 cut(s)	1324
TseFI	GTSAC	2 cut(s)	1084, 1118
TseI	GCWGC	3 cut(s)	9, 57, 494
Tsp45I	GTSAC	2 cut(s)	1084, 1118
TspDTI	ATGAA	4 cut(s)	555, 863, 887, 1178
TspGWI	ACGGA	1 cut(s)	494
TspRI	CASTG	1 cut(s)	1324
VpaK11BI	GGWCC	2 cut(s)	414, 760
XapI	RAATTY	1 cut(s)	565
XmiI	GTMKAC	1 cut(s)	261
Zsp2I	ATGCAT	1 cut(s)	540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.