pycom14g16720
MADS Family

MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012727
Physical Location & Seq
Forward (+)
18913301 .. 18916002
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGGGAAGGGTTAAGTTAAAGATAAAGAAATTAGAGAGCAGTGGTAACCGGCAGGTTACATTTTCAAAACGGAGAAATGGAATCTTGAAGAAAGCTAAGGAGTTGTCAATCTTATGTGATGTAGATATTGTCCTTCTCATGTTTTCGCCCACTGGAAGGCCTACGATTTTCCAAGGGGACCGCAGCTTTTGGAGCGACCCAGATAAGGTTGACAATCTTGAACAGCTTAGGCAGATGGAAGACATGCTTAAGGAATCAATCAACCGAACCTGTCTACGCAAGGAAAATTTGGGAAAACATCAACTAACATCACTAGATTGCACAAACCAGTTTCAAAATGGAATGCCTTCGTCTTTATTAGTTGGTGGTGGTATGCAAGAAGCTCAACCTATAACTTGGCTTTTCAATAGCAACAATCAACATATGATATTCCCGAATGAGCCGAACTATCTTCCACATAGAGGTGTGGAGTGCTCTACAAATGCCACTGTTCCACATTATACTAGTTACTTTGGCACTGGGACGAGCACTAGCCCTAGCACCAGCAAACAATCTGAGGCCGGTGATCCAGTACAACTAGATACTATGGGACAAATTTCTAATATGGAAGGAGGCGGTGGCTTAAATGAGTTTGACATTAATGCTTGTTTAAGTACGGAACCTGGTAAGCATTATGCTTATCCATCATATTGTAGTTCTTACGTGCCAGACGATCAGAAATTGAAGCATGAAATGGAAAAGAACGTACCAGCAAATCCGGTGGACTATCAACTAAGTAGCAACTTTGAACTGCCAACATCTTTGTATGAAACTGATCAGCATGCTTGGCTTTCTTCATCTGGGCCTAGCGGCATTGCTGTGTACAAGGAGAATACCTACCAACCGCAACCAAACGTGAGATTGTAG

Protein Analysis

302

Amino Acids

33.66

Weight (kDa)

6.0

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 11 - 55 1.3e-22 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 274
AciI CCGC 4 cut(s) 181, 615, 849, 884
AclWI GGATC 1 cut(s) 560
AcsI RAATTY 2 cut(s) 286, 594
AfaI GTAC 4 cut(s) 573, 655, 747, 863
AfiI CCNNNNNNNGG 3 cut(s) 156, 205, 461
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 7 cut(s) 66, 88, 221, 335, 406, 724, 788
AhlI ACTAGT 1 cut(s) 503
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 4 cut(s) 95, 186, 226, 383
AluI AGCT 4 cut(s) 95, 186, 226, 383
Alw21I GWGCWC 2 cut(s) 476, 530
AlwI GGATC 1 cut(s) 560
AoxI GGCC 3 cut(s) 158, 558, 842
ApeKI GCWGC 1 cut(s) 183
ApoI RAATTY 2 cut(s) 286, 594
AseI ATTAAT 1 cut(s) 639
Asp700I GAANNNNTTC 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 178, 842
AsuHPI GGTGA 1 cut(s) 575
AvaII GGWCC 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 2 cut(s) 476, 530
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 229, 691
BciT130I CCWGG 1 cut(s) 663
BclI TGATCA 1 cut(s) 814
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 6 cut(s) 314, 504, 531, 537, 578, 846
BfrI CTTAAG 1 cut(s) 248
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 2 cut(s) 184, 850
BlsI GCNGC 2 cut(s) 185, 851
Bme1390I CCNGG 1 cut(s) 663
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 178, 842
BmiI GGNNCC 2 cut(s) 179, 660
BmrFI CCNGG 1 cut(s) 663
BmrI ACTGGG 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 246
Bpu10I CCTNAGC 2 cut(s) 96, 227
BsaAI YACGTR 1 cut(s) 703
BsaJI CCNNGG 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 757
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 205, 461
Bse118I RCCGGY 2 cut(s) 48, 560
Bse1I ACTGG 4 cut(s) 157, 328, 523, 569
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 172
BseLI CCNNNNNNNGG 3 cut(s) 156, 205, 461
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 4 cut(s) 157, 328, 523, 569
BseXI GCAGC 1 cut(s) 195
BshFI GGCC 3 cut(s) 160, 560, 844
BsiHKAI GWGCWC 2 cut(s) 476, 530
BsiSI CCGG 3 cut(s) 49, 561, 758
BslFI GGGAC 3 cut(s) 191, 535, 603
BslI CCNNNNNNNGG 3 cut(s) 156, 205, 461
BsmFI GGGAC 3 cut(s) 191, 535, 603
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 3 cut(s) 160, 560, 844
Bsp1286I GDGCHC 2 cut(s) 476, 530
Bsp1407I TGTACA 1 cut(s) 861
Bsp143I GATC 3 cut(s) 565, 712, 814
BspACI CCGC 4 cut(s) 181, 615, 849, 884
BspANI GGCC 3 cut(s) 160, 560, 844
BspCNI CTCAG 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 179, 660
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 1 cut(s) 560
BspTI CTTAAG 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 2 cut(s) 48, 560
BsrGI TGTACA 1 cut(s) 861
BsrI ACTGG 4 cut(s) 157, 328, 523, 569
BssAI RCCGGY 2 cut(s) 48, 560
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 3 cut(s) 565, 712, 814
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 1 cut(s) 490
BstAFI CTTAAG 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 861
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 4 cut(s) 96, 227, 555, 773
BstEII GGTNACC 1 cut(s) 44
BstKTI GATC 3 cut(s) 568, 715, 817
BstMBI GATC 3 cut(s) 565, 712, 814
BstMWI GCNNNNNNNGC 2 cut(s) 192, 826
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 2 cut(s) 247, 824
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 661
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 246
BsuRI GGCC 3 cut(s) 160, 560, 844
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 4 cut(s) 46, 150, 486, 516
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 822
Cfr10I RCCGGY 2 cut(s) 48, 560
Cfr13I GGNCC 2 cut(s) 178, 842
CsiI ACCWGGT 1 cut(s) 661
Csp6I GTAC 4 cut(s) 572, 654, 746, 862
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviAII CATG 4 cut(s) 139, 244, 728, 821
CviQI GTAC 4 cut(s) 572, 654, 746, 862
DdeI CTNAG 4 cut(s) 96, 227, 555, 773
DpnI GATC 3 cut(s) 567, 714, 816
DpnII GATC 3 cut(s) 565, 712, 814
Eco130I CCWWGG 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 178
Eco91I GGTNACC 1 cut(s) 44
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 4 cut(s) 142, 247, 731, 824
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 3 cut(s) 191, 535, 603
FatI CATG 4 cut(s) 138, 243, 727, 820
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 184, 850
Fsp4HI GCNGC 2 cut(s) 184, 850
FspBI CTAG 6 cut(s) 314, 504, 531, 537, 578, 846
GluI GCNGC 2 cut(s) 184, 850
HaeIII GGCC 3 cut(s) 160, 560, 844
HapII CCGG 3 cut(s) 49, 561, 758
Hin1II CATG 4 cut(s) 142, 247, 731, 824
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 81, 254
HpaII CCGG 3 cut(s) 49, 561, 758
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 4 cut(s) 211, 275, 763, 862
Hpy188I TCNGA 2 cut(s) 556, 717
Hpy188III TCNNGA 3 cut(s) 85, 218, 433
Hpy8I GTNNAC 4 cut(s) 211, 275, 763, 862
HpyAV CCTTC 4 cut(s) 143, 150, 357, 602
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 3 cut(s) 702, 744, 894
HpyCH4V TGCA 2 cut(s) 321, 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 826
HpyF3I CTNAG 4 cut(s) 96, 227, 555, 773
HpySE526I ACGT 3 cut(s) 702, 744, 894
Hsp92II CATG 4 cut(s) 142, 247, 731, 824
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 3 cut(s) 565, 712, 814
LmnI GCTCC 1 cut(s) 192
Lsp1109I GCAGC 1 cut(s) 195
MabI ACCWGGT 1 cut(s) 661
MaeI CTAG 6 cut(s) 314, 504, 531, 537, 578, 846
MaeII ACGT 3 cut(s) 702, 744, 894
MaeIII GTNAC 3 cut(s) 44, 55, 506
MalI GATC 3 cut(s) 567, 714, 816
MboI GATC 3 cut(s) 565, 712, 814
MboII GAAGA 4 cut(s) 100, 251, 443, 825
MhlI GDGCHC 2 cut(s) 476, 530
MluCI AATT 4 cut(s) 29, 286, 594, 719
MnlI CCTC 3 cut(s) 455, 550, 605
MroXI GAANNNNTTC 1 cut(s) 346
MseI TTAA 6 cut(s) 12, 17, 249, 623, 639, 650
MslI CAYNNNNRTG 2 cut(s) 462, 857
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 3 cut(s) 49, 561, 758
MspR9I CCNGG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 2 cut(s) 192, 826
NdeI CATATG 1 cut(s) 423
NdeII GATC 3 cut(s) 565, 712, 814
NlaIII CATG 4 cut(s) 142, 247, 731, 824
NlaIV GGNNCC 2 cut(s) 179, 660
NspI RCATGY 2 cut(s) 247, 824
PaeI GCATGC 1 cut(s) 824
PceI AGGCCT 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 346
PfeI GAWTC 2 cut(s) 81, 254
PkrI GCNGC 2 cut(s) 185, 851
Ppu21I YACGTR 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 661
PspEI GGTNACC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 2 cut(s) 179, 660
PspPI GGNCC 2 cut(s) 178, 842
RsaI GTAC 4 cut(s) 573, 655, 747, 863
RsaNI GTAC 4 cut(s) 572, 654, 746, 862
RseI CAYNNNNRTG 2 cut(s) 462, 857
SaqAI TTAA 6 cut(s) 12, 17, 249, 623, 639, 650
SatI GCNGC 2 cut(s) 184, 850
Sau3AI GATC 3 cut(s) 565, 712, 814
Sau96I GGNCC 2 cut(s) 178, 842
ScrFI CCNGG 1 cut(s) 663
SduI GDGCHC 2 cut(s) 476, 530
SexAI ACCWGGT 1 cut(s) 661
SinI GGWCC 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 462, 857
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SpeI ACTAGT 1 cut(s) 503
SphI GCATGC 1 cut(s) 824
Sse9I AATT 4 cut(s) 29, 286, 594, 719
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 4 cut(s) 181, 615, 849, 884
SspMI CTAG 6 cut(s) 314, 504, 531, 537, 578, 846
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 3 cut(s) 705, 747, 897
TasI AATT 4 cut(s) 29, 286, 594, 719
TatI WGTACW 2 cut(s) 571, 861
TauI GCSGC 1 cut(s) 852
TfiI GAWTC 2 cut(s) 81, 254
Tru1I TTAA 6 cut(s) 12, 17, 249, 623, 639, 650
Tru9I TTAA 6 cut(s) 12, 17, 249, 623, 639, 650
TscAI CASTG 4 cut(s) 46, 157, 493, 523
TseI GCWGC 1 cut(s) 183
TspDTI ATGAA 3 cut(s) 744, 822, 825
TspGWI ACGGA 2 cut(s) 85, 671
TspRI CASTG 4 cut(s) 46, 157, 493, 523
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 178
VspI ATTAAT 1 cut(s) 639
XapI RAATTY 2 cut(s) 286, 594
XceI RCATGY 2 cut(s) 247, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 346
XspI CTAG 6 cut(s) 314, 504, 531, 537, 578, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.