pycom14g19830

Protein of unknown function (DUF3007)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007172
Physical Location & Seq
Forward (+)
21054063 .. 21056278
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGCGCTTAAAGGCCAGAGCTTTTCATGGTTTGGTGAGAGGGGGAGTTATGACTCACAGCTTATATAGAGAGGAGACATCTCTTTTTATCCGAGAAGGATCCAATAGCATCAACCGCATTATGCAAAAAAGGGTGCTCTTGACCATCCCCAGGAATAGCAGTTCGTCGGACTCTAATAGCAGTGAGGATGACTCCTCTGATCAGGCCAAGAAAAAACCGTTCGGATACTCAAGGAAGGATGTTTTATTGATTGGACTTGGAGTAACTGCTATAGGGATCGGCTTGAAAAGTGGATTAGAGTTTGCTGGAGTTGATCCTTTACAAGCAGGGAACGTCGTTCAGCTGGTGCTGGTGTTGGGCCTGACAATTGGATGGATTTCCACGTATATTTTTCGAGTTTCAAATAAAGAAATGACATATGCACAGCAAATACGCGACTATGAAAGCAAAGTCATGGAGAAGAGGTTAGAAGGCCTGACGGAAGCAGAGCTAGTAGCATTGCTTGAACAAGTTGAGGAAGAGAAGAGGCGCCTCGGCGAGCAGCAGGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3007

Protein Analysis

185

Amino Acids

20.56

Weight (kDa)

9.12

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3007 PF11460 77 - 172 3.9e-33 Protein of unknown function (DUF3007)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 528
AccII CGCG 1 cut(s) 435
AciI CCGC 1 cut(s) 115
AclWI GGATC 4 cut(s) 93, 106, 284, 308
AcyI GRCGYC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 3 cut(s) 286, 402, 506
AjnI CCWGG 1 cut(s) 149
AloI GAACNNNNNNTCC 2 cut(s) 145, 177
AluBI AGCT 4 cut(s) 20, 60, 343, 490
AluI AGCT 4 cut(s) 20, 60, 343, 490
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 4 cut(s) 93, 106, 284, 308
AoxI GGCC 4 cut(s) 12, 204, 358, 472
ApeKI GCWGC 1 cut(s) 541
AspLEI GCGC 2 cut(s) 6, 531
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 46
BamHI GGATCC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 138
BccI CCATC 2 cut(s) 152, 366
BciT130I CCWGG 1 cut(s) 151
BciVI GTATCC 1 cut(s) 218
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 1 cut(s) 535
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 1 cut(s) 542
BlsI GCNGC 1 cut(s) 543
Bme1390I CCNGG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 100, 530
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 529
BsaJI CCNNGG 2 cut(s) 149, 532
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 497
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 2 cut(s) 149, 532
BseGI GGATG 4 cut(s) 144, 193, 244, 377
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 497
BseRI GAGGAG 2 cut(s) 86, 184
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 4 cut(s) 14, 206, 360, 474
BshNI GGYRCC 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 1 cut(s) 68
BsnI GGCC 4 cut(s) 14, 206, 360, 474
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 4 cut(s) 98, 199, 276, 313
BspACI CCGC 1 cut(s) 115
BspANI GGCC 4 cut(s) 14, 206, 360, 474
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 100, 530
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 4 cut(s) 93, 106, 284, 308
BspT107I GGYRCC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 149, 532
BssMI GATC 4 cut(s) 98, 199, 276, 313
BssNI GRCGYC 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 3 cut(s) 455, 513, 518
BstACI GRCGYC 1 cut(s) 529
BstBAI YACGTR 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 539
BstF5I GGATG 4 cut(s) 144, 193, 244, 377
BstFNI CGCG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 2 cut(s) 6, 531
BstKTI GATC 4 cut(s) 101, 202, 279, 316
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 4 cut(s) 98, 199, 276, 313
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 4 cut(s) 14, 206, 360, 474
BtsCI GGATG 4 cut(s) 144, 193, 244, 377
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 539
CfoI GCGC 2 cut(s) 6, 531
Cfr13I GGNCC 1 cut(s) 358
CviAII CATG 2 cut(s) 26, 454
CviJI RGCY 9 cut(s) 14, 20, 60, 206, 282, 343, 360, 474, 490
CviKI_1 RGCY 9 cut(s) 14, 20, 60, 206, 282, 343, 360, 474, 490
DinI GGCGCC 1 cut(s) 530
DpnI GATC 4 cut(s) 100, 201, 278, 315
DpnII GATC 4 cut(s) 98, 199, 276, 313
Eam1104I CTCTTC 3 cut(s) 455, 513, 518
EarI CTCTTC 3 cut(s) 455, 513, 518
Eco147I AGGCCT 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 530
EheI GGCGCC 1 cut(s) 530
FaeI CATG 2 cut(s) 29, 457
FatI CATG 2 cut(s) 25, 453
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 542
FokI GGATG 4 cut(s) 131, 200, 251, 384
Fsp4HI GCNGC 1 cut(s) 542
FspBI CTAG 1 cut(s) 491
GlaI GCGC 2 cut(s) 5, 530
GluI GCNGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 4 cut(s) 14, 206, 360, 474
HhaI GCGC 2 cut(s) 6, 531
Hin1I GRCGYC 1 cut(s) 529
Hin1II CATG 2 cut(s) 29, 457
Hin6I GCGC 2 cut(s) 4, 529
HinP1I GCGC 2 cut(s) 4, 529
HinfI GANTC 3 cut(s) 52, 170, 191
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 4 cut(s) 92, 169, 199, 224
Hpy188III TCNNGA 1 cut(s) 139
Hpy99I CGWCG 2 cut(s) 169, 338
HpyAV CCTTC 3 cut(s) 89, 229, 464
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 2 cut(s) 333, 383
HpyCH4V TGCA 2 cut(s) 124, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpySE526I ACGT 2 cut(s) 333, 383
Hsp92I GRCGYC 1 cut(s) 529
Hsp92II CATG 2 cut(s) 29, 457
HspAI GCGC 2 cut(s) 4, 529
KasI GGCGCC 1 cut(s) 528
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 4 cut(s) 98, 199, 276, 313
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 491
MaeII ACGT 2 cut(s) 333, 383
MaeIII GTNAC 1 cut(s) 262
MalI GATC 4 cut(s) 100, 201, 278, 315
MboI GATC 4 cut(s) 98, 199, 276, 313
MboII GAAGA 3 cut(s) 472, 530, 535
MfeI CAATTG 1 cut(s) 366
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 1 cut(s) 366
Mly113I GGCGCC 1 cut(s) 529
MlyI GAGTC 3 cut(s) 46, 164, 185
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 8 cut(s) 32, 64, 178, 205, 456, 508, 519, 542
MseI TTAA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 151
MunI CAATTG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 151
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 114
NarI GGCGCC 1 cut(s) 529
NdeI CATATG 1 cut(s) 418
NdeII GATC 4 cut(s) 98, 199, 276, 313
NlaIII CATG 2 cut(s) 29, 457
NlaIV GGNNCC 2 cut(s) 100, 530
NmeAIII GCCGAG 1 cut(s) 513
PceI AGGCCT 1 cut(s) 474
PkrI GCNGC 1 cut(s) 543
PleI GAGTC 3 cut(s) 46, 164, 185
PluTI GGCGCC 1 cut(s) 532
PpsI GAGTC 3 cut(s) 46, 164, 185
Ppu21I YACGTR 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 100, 530
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 98
PvuII CAGCTG 1 cut(s) 343
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 1 cut(s) 542
Sau3AI GATC 4 cut(s) 98, 199, 276, 313
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 3 cut(s) 46, 164, 185
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 138
SetI ASST 8 cut(s) 22, 62, 336, 345, 386, 467, 492, 549
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 270
SfoI GGCGCC 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 1 cut(s) 366
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 1 cut(s) 115
SspDI GGCGCC 1 cut(s) 528
SspMI CTAG 1 cut(s) 491
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 2 cut(s) 336, 386
TaqI TCGA 1 cut(s) 394
TasI AATT 1 cut(s) 366
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 1 cut(s) 187
TseI GCWGC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 14, 456
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 187
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.