pycom14g19970

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011991
Physical Location & Seq
Reverse (-)
21126581 .. 21127885
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGGGTCTCCTTCTCTCAAAAAATATTTTGGTGAATTTGATTTGCAATTCTTCTAATTCTCCCGTTTTCTCCAAGAAAAATGGCGGAGATTTCATCCCCATGCAAATTGAGAACATGGTTTCTTTGGTTTCCCGGACCGGAAGACAATTCCAGCGCTATGAAGAAGGTTGCCGCCAAGTTGTAGGATGCATACCATACAGATATCGGGAAAAACAAACTTCATCTGATGAGGAACTGGAAGTTCTTGTGATTAGTTCACAGAAGGGGAAAGGAATGTTGTTCCCAAAGGGAGGTTGGGAAATGGATGAGTCAATTGAAGAGGCGGCGAAAAGAGAGACGCTTGAGGAAGCTGGTGTGACTGGCCATCTTGAAACTAGATTGGGATCGTGGCGTTACAAGAGCAAAACCCAAGGCACGGTTCATGAGGGGTACATGTTCCCTTTGCTTGTTCAGCAACAGTTGGAACTTTGGCCCGAGAAAAGTTCCCGAAAACGTGAATGGGTGACGATCTCTGAAGCTAGAGAAGCATGTCAAAATTGGTGGATGAAGGAAGCTTTGGAGAAATTAGTTTGTCAAAAAATGCAGCCCCAACCGAAAGGAGAGGAAGGAGAAGCAACTTGTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

209

Amino Acids

23.82

Weight (kDa)

6.23

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 76 - 188 5.7e-14 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18300 AT1G73540
fragaria_vesca FvH4_5g17620 FvH4_5g17620
malus_domestica MD06G1232000.v1.1 MD14G1238600.v1.1
prunus_persica Prupe.5G235900_v2.0.a1
pyrus_communis pycom06g20590 pycom14g19970
rosa_chinensis RchiOBHm_Chr7g0178041
rosa_laevigata RLG00000005479
rosa_multiflora Rmu_sc0000425.1_g000002
rosa_roxburghii Rroxscaffold_3G00275250
rosa_rugosa Rorug06G0412500 Rorug06G0412500
rosa_samantha Rh7AG012100 Rh7CG012100 Rh7DG012400
rosa_wichuraiana Rw7G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 84, 172, 323
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 2 cut(s) 431, 622
AfeI AGCGCT 1 cut(s) 155
AfiI CCNNNNNNNGG 2 cut(s) 290, 415
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 2 cut(s) 317, 371
AjuI GAANNNNNNNTTGG 4 cut(s) 402, 434, 539, 571
AluBI AGCT 3 cut(s) 350, 518, 554
AluI AGCT 3 cut(s) 350, 518, 554
Alw26I GTCTC 2 cut(s) 11, 329
AlwI GGATC 1 cut(s) 391
Ama87I CYCGRG 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 155
AoxI GGCC 2 cut(s) 361, 470
ApeKI GCWGC 1 cut(s) 583
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 135, 471
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 43, 514
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 595
BccI CCATC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 11, 329
BfaI CTAG 2 cut(s) 375, 519
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 3 cut(s) 172, 324, 584
BlsI GCNGC 3 cut(s) 173, 325, 585
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 135
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 2 cut(s) 135, 471
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 415
Bse1I ACTGG 2 cut(s) 240, 364
Bse8I GATNNNNATC 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 4 cut(s) 93, 191, 310, 549
BseJI GATNNNNATC 1 cut(s) 382
BseLI CCNNNNNNNGG 2 cut(s) 290, 415
BseNI ACTGG 2 cut(s) 240, 364
BseXI GCAGC 1 cut(s) 595
BshFI GGCC 2 cut(s) 363, 472
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 2 cut(s) 133, 138
BslI CCNNNNNNNGG 2 cut(s) 290, 415
BsmAI GTCTC 2 cut(s) 11, 329
BsmBI CGTCTC 1 cut(s) 329
BsnI GGCC 2 cut(s) 363, 472
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 473
Bsp1407I TGTACA 1 cut(s) 620
Bsp143I GATC 2 cut(s) 383, 507
BspACI CCGC 3 cut(s) 84, 172, 323
BspANI GGCC 2 cut(s) 363, 472
BspHI TCATGA 1 cut(s) 421
BspPI GGATC 1 cut(s) 391
BspTNI GGTCTC 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 620
BsrI ACTGG 2 cut(s) 240, 364
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 2 cut(s) 383, 507
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 418, 459
Bst6I CTCTTC 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 620
BstF5I GGATG 4 cut(s) 93, 191, 310, 549
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 2 cut(s) 386, 510
BstMAI GTCTC 2 cut(s) 11, 329
BstMBI GATC 2 cut(s) 383, 507
BstMWI GCNNNNNNNGC 2 cut(s) 451, 524
BstNSI RCATGY 2 cut(s) 436, 531
BstSCI CCNGG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 1 cut(s) 148
BsuRI GGCC 2 cut(s) 363, 472
BtsCI GGATG 4 cut(s) 93, 191, 310, 549
CciI TCATGA 1 cut(s) 421
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 135, 471
CpoI CGGWCCG 1 cut(s) 135
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 430, 621
CspCI CAANNNNNGTGG 2 cut(s) 521, 556
CspI CGGWCCG 1 cut(s) 135
CviAII CATG 5 cut(s) 100, 115, 422, 433, 528
CviJI RGCY 6 cut(s) 350, 363, 472, 518, 554, 586
CviKI_1 RGCY 6 cut(s) 350, 363, 472, 518, 554, 586
CviQI GTAC 2 cut(s) 430, 621
DpnI GATC 2 cut(s) 385, 509
DpnII GATC 2 cut(s) 383, 507
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 135
Eco47III AGCGCT 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 473
EcoRV GATATC 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 5 cut(s) 103, 118, 425, 436, 531
FaiI YATR 9 cut(s) 101, 116, 159, 191, 196, 423, 434, 529, 625
FatI CATG 5 cut(s) 99, 114, 421, 432, 527
Fnu4HI GCNGC 3 cut(s) 172, 324, 584
FokI GGATG 4 cut(s) 80, 198, 317, 556
Fsp4HI GCNGC 3 cut(s) 172, 324, 584
FspBI CTAG 2 cut(s) 375, 519
GlaI GCGC 1 cut(s) 155
GluI GCNGC 3 cut(s) 172, 324, 584
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 363, 472
HapII CCGG 2 cut(s) 133, 138
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 156
Hin1II CATG 5 cut(s) 103, 118, 425, 436, 531
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 1 cut(s) 308
HpaII CCGG 2 cut(s) 133, 138
HphI GGTGA 2 cut(s) 43, 514
Hpy166II GTNNAC 1 cut(s) 257
Hpy188I TCNGA 2 cut(s) 226, 514
Hpy188III TCNNGA 4 cut(s) 206, 368, 422, 486
Hpy8I GTNNAC 1 cut(s) 257
HpyAV CCTTC 5 cut(s) 20, 158, 256, 541, 599
HpyCH4III ACNGT 2 cut(s) 418, 459
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 4 cut(s) 45, 103, 189, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 524
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 5 cut(s) 103, 118, 425, 436, 531
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 383, 507
LpnPI CCDG 6 cut(s) 146, 151, 164, 221, 336, 345
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 1 cut(s) 176
MaeI CTAG 2 cut(s) 375, 519
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 3 cut(s) 355, 392, 502
MalI GATC 2 cut(s) 385, 509
MboI GATC 2 cut(s) 383, 507
MboII GAAGA 4 cut(s) 42, 153, 173, 329
MfeI CAATTG 1 cut(s) 312
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 8 cut(s) 34, 46, 55, 105, 146, 312, 535, 563
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 6 cut(s) 223, 284, 313, 337, 418, 595
Mox20I TGGCCA 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 191
MscI TGGCCA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 2 cut(s) 133, 138
MspR9I CCNGG 1 cut(s) 133
MunI CAATTG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 451, 524
NciI CCSGG 1 cut(s) 133
NdeII GATC 2 cut(s) 383, 507
NlaIII CATG 5 cut(s) 103, 118, 425, 436, 531
NmuCI GTSAC 2 cut(s) 355, 502
NsiI ATGCAT 1 cut(s) 191
NspI RCATGY 2 cut(s) 436, 531
PagI TCATGA 1 cut(s) 421
PciI ACATGT 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 3 cut(s) 173, 325, 585
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PscI ACATGT 1 cut(s) 432
PspPI GGNCC 2 cut(s) 135, 471
RsaI GTAC 2 cut(s) 431, 622
RsaNI GTAC 2 cut(s) 430, 621
RseI CAYNNNNRTG 1 cut(s) 98
Rsr2I CGGWCCG 1 cut(s) 135
RsrII CGGWCCG 1 cut(s) 135
SatI GCNGC 3 cut(s) 172, 324, 584
Sau3AI GATC 2 cut(s) 383, 507
Sau96I GGNCC 2 cut(s) 135, 471
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 133
SetI ASST 6 cut(s) 169, 295, 352, 496, 520, 556
SfaNI GCATC 1 cut(s) 176
SinI GGWCC 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 8 cut(s) 34, 46, 55, 105, 146, 312, 535, 563
SsiI CCGC 3 cut(s) 84, 172, 323
SspI AATATT 1 cut(s) 25
SspMI CTAG 2 cut(s) 375, 519
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 418, 459
TaiI ACGT 1 cut(s) 496
TasI AATT 8 cut(s) 34, 46, 55, 105, 146, 312, 535, 563
TatI WGTACW 1 cut(s) 620
TauI GCSGC 2 cut(s) 174, 326
TseFI GTSAC 2 cut(s) 355, 502
TseI GCWGC 1 cut(s) 583
Tsp45I GTSAC 2 cut(s) 355, 502
TspDTI ATGAA 5 cut(s) 82, 174, 210, 410, 560
VpaK11BI GGWCC 1 cut(s) 135
XapI RAATTY 1 cut(s) 34
XceI RCATGY 2 cut(s) 436, 531
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XspI CTAG 2 cut(s) 375, 519
Zsp2I ATGCAT 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.