pycom14g20190

DNA-directed RNA-polymerase II subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002628
Physical Location & Seq
Forward (+)
21264094 .. 21266685
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGTTGGCAGTTCTAAGATAATGTCAGGTCGGCCCAAAACTGAGGTCGGTTCGGACACTCGAGAGCTTTATATAACAGCTGAAAGCCCCAAATACATAATTCAATTTCTCATTAGGGATTTGCTTACGTTCCAGAAAGCATATCTAATTGGGGTCACGTTGATTGTCAAGAAACAAACGTCCATAGCTCTCTCAAAAATGCTAAGAGCAGAAGATGAAGACGCTTCAGAACTCAAACTTGGAGACGATTTTTCGAAGGCGAAGTGTCTGATGAATAGTGAGGTTGCCATTCTTCTTGAGCACAGATGTGATCAGATGAAGCATATGTCAGGCGAATCAATGCACCAACTTCCCCAAGTGCTGGAGAAATCACTGCAGTATGTTAAGCGCTTCAGTCGCTTCACGAATCAGGGCTCTGTGAAGCAAGTTCGAGAAGTTCTCGGTAGATACCAGTTGGCTGAATTCGAGCTTGCTGTGATTGGAAATCTTTGTCCTGAAACTGTGGAGGAAGCCAAGGCAGTTGTGCCTTCCCTCAAGACGAAAGGACGTGGGCACGATGATGAAGCCATTGACAGACTGTTGAATGACCTAATGATGATTAAAAAGTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb4

Protein Analysis

205

Amino Acids

23.11

Weight (kDa)

7.69

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb4 PF03874 89 - 197 1.3e-25 RNA polymerase Rpb4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 361
AcsI RAATTY 1 cut(s) 461
AcuI CTGAAG 2 cut(s) 210, 376
AfeI AGCGCT 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 2 cut(s) 104, 583
AjiI CACGTC 1 cut(s) 548
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AleI CACNNNNGTG 1 cut(s) 306
AluBI AGCT 4 cut(s) 67, 80, 188, 469
AluI AGCT 4 cut(s) 67, 80, 188, 469
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 60
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 33
AsuII TTCGAA 1 cut(s) 254
AvaI CYCGRG 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 555
BanII GRGCYC 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 303
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 423, 455
BpmI CTGGAG 1 cut(s) 383
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 317, 518
BsaJI CCNNGG 1 cut(s) 513
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 451
BseDI CCNNGG 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 451
BseSI GKGCMC 1 cut(s) 555
BshFI GGCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 1 cut(s) 237
BsmBI CGTCTC 1 cut(s) 237
BsnI GGCC 1 cut(s) 34
BsoBI CYCGRG 1 cut(s) 60
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 3 cut(s) 303, 416, 555
Bsp143I GATC 1 cut(s) 310
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 1 cut(s) 34
BspMAI CTGCAG 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 254
BsrI ACTGG 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 502, 579
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 3 cut(s) 15, 42, 203
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstSFI CTRYAG 1 cut(s) 374
BstSLI GKGCMC 1 cut(s) 555
BstV2I GAAGAC 1 cut(s) 225
BsuRI GGCC 1 cut(s) 34
BtrI CACGTC 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 371
Cac8I GCNNGC 1 cut(s) 471
CfoI GCGC 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 33
CseI GACGC 1 cut(s) 230
DdeI CTNAG 3 cut(s) 15, 42, 203
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 416
Eco47III AGCGCT 1 cut(s) 389
Eco57I CTGAAG 2 cut(s) 210, 376
Eco88I CYCGRG 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 237
FaiI YATR 8 cut(s) 72, 74, 98, 142, 185, 324, 326, 381
FauNDI CATATG 1 cut(s) 324
FbaI TGATCA 1 cut(s) 310
FriOI GRGCYC 1 cut(s) 416
GlaI GCGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 383
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 1 cut(s) 34
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 390
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HinfI GANTC 2 cut(s) 335, 406
Hpy188I TCNGA 4 cut(s) 55, 229, 270, 315
Hpy188III TCNNGA 8 cut(s) 62, 133, 169, 296, 403, 431, 494, 535
HpyAV CCTTC 2 cut(s) 250, 537
HpyCH4III ACNGT 2 cut(s) 502, 579
HpyCH4IV ACGT 4 cut(s) 128, 158, 179, 547
HpyCH4V TGCA 2 cut(s) 343, 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 3 cut(s) 15, 42, 203
HpySE526I ACGT 4 cut(s) 128, 158, 179, 547
HspAI GCGC 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 12, 146, 315, 347, 395, 464, 507
MaeII ACGT 4 cut(s) 128, 158, 179, 547
MaeIII GTNAC 1 cut(s) 154
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 3 cut(s) 224, 230, 284
MhlI GDGCHC 3 cut(s) 303, 416, 555
MluCI AATT 4 cut(s) 99, 104, 147, 461
MnlI CCTC 4 cut(s) 37, 274, 499, 542
MseI TTAA 2 cut(s) 384, 600
MslI CAYNNNNRTG 2 cut(s) 306, 558
MspA1I CMGCKG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 396
NdeI CATATG 1 cut(s) 324
NdeII GATC 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 154
NspV TTCGAA 1 cut(s) 254
OliI CACNNNNGTG 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 60
PfeI GAWTC 2 cut(s) 335, 406
PflMI CCANNNNNTGG 1 cut(s) 361
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 378
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 2 cut(s) 306, 558
SaqAI TTAA 2 cut(s) 384, 600
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 33
SduI GDGCHC 3 cut(s) 303, 416, 555
SfcI CTRYAG 1 cut(s) 374
Sfr274I CTCGAG 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 306, 558
SmlI CTYRAG 3 cut(s) 60, 296, 533
SmoI CTYRAG 3 cut(s) 60, 296, 533
Sse9I AATT 4 cut(s) 99, 104, 147, 461
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 502, 579
TaiI ACGT 4 cut(s) 131, 161, 182, 550
TaqI TCGA 4 cut(s) 61, 254, 430, 465
TasI AATT 4 cut(s) 99, 104, 147, 461
TfiI GAWTC 2 cut(s) 335, 406
Tru1I TTAA 2 cut(s) 384, 600
Tru9I TTAA 2 cut(s) 384, 600
TscAI CASTG 1 cut(s) 378
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 4 cut(s) 231, 287, 332, 576
TspRI CASTG 1 cut(s) 378
Van91I CCANNNNNTGG 1 cut(s) 361
XapI RAATTY 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.