Rw7G000780

DNA-directed RNA-polymerase II subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002628
Physical Location & Seq
Reverse (-)
639226 .. 641086
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000780.1

Sequence Viewer

Length: 414 bp
ATGCTCAAAGGAGAAGATGAAGACGCTTCAGAGCTCAAAATCGGAGAGGATTTTCTGAAGGCAAAATGTCTGATGAATAGTGAGGTAGCCATTCTTCTTGAGCATAGATATGATCAGATGAAGCAAATGTCTGGCGATTCAACTCCACTTCCCCAAGTGTTGGAGAAATCGCTGCAGTATGTCAAGTGCTTCAGTCGGTTCACGAATCAAGGCTCTGTAAAGCAAGTTCGAGAGGTCCTCAGTAGATACCAGTTGGCTGAATTTGAGCTGGCTGTGATCGGGAATCTTTGCCCTGAAACTGTGGAGGAGGCTAAGGCAATTGTGCCCTCACTCAAGACCAAAGGACGGGGGCATGATGATGAAGCCGTTGAGAGATTGTTGAATGACCTATTAATGATTAAAAAGTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb4

Protein Analysis

137

Amino Acids

15.56

Weight (kDa)

5.19

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb4 PF03874 23 - 130 6.3e-25 RNA polymerase Rpb4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 160
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 3 cut(s) 12, 77, 175
AfiI CCNNNNNNNGG 2 cut(s) 160, 345
AgsI TTSAA 2 cut(s) 141, 382
AluBI AGCT 2 cut(s) 34, 268
AluI AGCT 2 cut(s) 34, 268
Alw21I GWGCWC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 1 cut(s) 260
AseI ATTAAT 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 36
BarI GAAGNNNNNNTAC 2 cut(s) 78, 110
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 159
BceAI ACGGC 1 cut(s) 350
BclI TGATCA 1 cut(s) 112
BfmI CTRYAG 1 cut(s) 173
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 27
BplI GAGNNNNNCTC 2 cut(s) 222, 254
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 119, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 345
Bse1I ACTGG 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 160, 345
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 160, 345
Bsp1286I GDGCHC 2 cut(s) 36, 327
Bsp143I GATC 2 cut(s) 112, 276
BspCNI CTCAG 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 250
BssMI GATC 2 cut(s) 112, 276
Bst4CI ACNGT 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 2 cut(s) 239, 312
BstKTI GATC 2 cut(s) 115, 279
BstMBI GATC 2 cut(s) 112, 276
BstSFI CTRYAG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 32
CviAII CATG 1 cut(s) 353
CviJI RGCY 8 cut(s) 34, 89, 213, 257, 268, 272, 311, 365
CviKI_1 RGCY 8 cut(s) 34, 89, 213, 257, 268, 272, 311, 365
DdeI CTNAG 2 cut(s) 239, 312
DpnI GATC 2 cut(s) 114, 278
DpnII GATC 2 cut(s) 112, 276
Ecl136II GAGCTC 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 3 cut(s) 12, 77, 175
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 36
FaeI CATG 1 cut(s) 356
FaiI YATR 4 cut(s) 105, 111, 180, 354
FatI CATG 1 cut(s) 352
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 173
HgaI GACGC 1 cut(s) 32
Hin1II CATG 1 cut(s) 356
HinfI GANTC 3 cut(s) 137, 205, 283
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 5 cut(s) 31, 44, 57, 72, 117
Hpy188III TCNNGA 5 cut(s) 98, 202, 230, 280, 334
Hpy8I GTNNAC 1 cut(s) 201
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 1 cut(s) 175
HpyF3I CTNAG 2 cut(s) 239, 312
Hsp92II CATG 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 2 cut(s) 112, 276
LpnPI CCDG 4 cut(s) 117, 254, 263, 306
Lsp1109I GCAGC 1 cut(s) 159
MalI GATC 2 cut(s) 114, 278
MboI GATC 2 cut(s) 112, 276
MboII GAAGA 3 cut(s) 26, 32, 86
MfeI CAATTG 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 36, 327
MluCI AATT 2 cut(s) 260, 318
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 7 cut(s) 40, 76, 226, 248, 298, 301, 337
MseI TTAA 2 cut(s) 392, 399
MslI CAYNNNNRTG 2 cut(s) 108, 357
MunI CAATTG 1 cut(s) 318
NdeII GATC 2 cut(s) 112, 276
NlaIII CATG 1 cut(s) 356
PfeI GAWTC 3 cut(s) 137, 205, 283
PflMI CCANNNNNTGG 1 cut(s) 160
PkrI GCNGC 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 1 cut(s) 235
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
PstI CTGCAG 1 cut(s) 177
RseI CAYNNNNRTG 2 cut(s) 108, 357
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 392, 399
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 2 cut(s) 112, 276
Sau96I GGNCC 1 cut(s) 235
SduI GDGCHC 2 cut(s) 36, 327
SetI ASST 5 cut(s) 36, 87, 237, 270, 390
SfcI CTRYAG 1 cut(s) 173
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 108, 357
SmlI CTYRAG 2 cut(s) 98, 332
SmoI CTYRAG 2 cut(s) 98, 332
Sse9I AATT 2 cut(s) 260, 318
SstI GAGCTC 1 cut(s) 36
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 1 cut(s) 229
TasI AATT 2 cut(s) 260, 318
TfiI GAWTC 3 cut(s) 137, 205, 283
Tru1I TTAA 2 cut(s) 392, 399
Tru9I TTAA 2 cut(s) 392, 399
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 4 cut(s) 33, 89, 134, 375
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 235
VspI ATTAAT 1 cut(s) 392
XapI RAATTY 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.