Rh7CG009600

DNA-directed RNA-polymerase II subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002628
Physical Location & Seq
Reverse (-)
764070 .. 766305
2236 bp
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UTR
Exon/CDS
Intron
Rh7CG009600.1

Sequence Viewer

Length: 342 bp
ATGAATAGTGAGGTAGCCATTCTTCTTGAGCATAGATATGATCAGATGAAGCAAATGTCTGGCGATTCAACTCCACTTCCCCAAGTGTTCGAGAAATCGCTGCAGTATGTCAAGTGCTTCAGTCGGTTCACGAATCAAGGCTCTGTAAAGCAAGTTCGAGAGGTCCTCAGTAGATACCAGTTGGCTGAATTTGAGCTGGCTGTGATCGGGAATCTTTGCCCTGAAACTGTGGAGGAGGCTAAGGCAATTGTGCCCTCACTCAAGACCAAAGGACGGGGGCATGATGATGAAGCCGTTGAGAGATTGTTGAATGACCTATTAATGATAAAAAAGTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb4

Protein Analysis

113

Amino Acids

12.93

Weight (kDa)

5.74

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb4 PF03874 1 - 106 5.8e-24 RNA polymerase Rpb4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 2 cut(s) 69, 310
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 188
AseI ATTAAT 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 163
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 255
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 278
BclI TGATCA 1 cut(s) 40
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 47, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 40, 204
BspCNI CTCAG 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 105
BsrI ACTGG 1 cut(s) 178
BssMI GATC 2 cut(s) 40, 204
Bst4CI ACNGT 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 167, 240
BstKTI GATC 2 cut(s) 43, 207
BstMBI GATC 2 cut(s) 40, 204
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 163
CviAII CATG 1 cut(s) 281
CviJI RGCY 7 cut(s) 17, 141, 185, 196, 200, 239, 293
CviKI_1 RGCY 7 cut(s) 17, 141, 185, 196, 200, 239, 293
DdeI CTNAG 2 cut(s) 167, 240
DpnI GATC 2 cut(s) 42, 206
DpnII GATC 2 cut(s) 40, 204
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 163
FaeI CATG 1 cut(s) 284
FaiI YATR 4 cut(s) 33, 39, 108, 282
FatI CATG 1 cut(s) 280
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 101
Hin1II CATG 1 cut(s) 284
HinfI GANTC 3 cut(s) 65, 133, 211
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 6 cut(s) 26, 91, 130, 158, 208, 262
Hpy8I GTNNAC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 1 cut(s) 103
HpyF3I CTNAG 2 cut(s) 167, 240
Hsp92II CATG 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 40, 204
LpnPI CCDG 4 cut(s) 45, 182, 191, 234
Lsp1109I GCAGC 1 cut(s) 87
MalI GATC 2 cut(s) 42, 206
MboI GATC 2 cut(s) 40, 204
MboII GAAGA 1 cut(s) 14
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 2 cut(s) 188, 246
MnlI CCTC 6 cut(s) 4, 154, 176, 226, 229, 265
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 2 cut(s) 36, 285
MunI CAATTG 1 cut(s) 246
NdeII GATC 2 cut(s) 40, 204
NlaIII CATG 1 cut(s) 284
PfeI GAWTC 3 cut(s) 65, 133, 211
PkrI GCNGC 1 cut(s) 102
PpuMI RGGWCCY 1 cut(s) 163
PshBI ATTAAT 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 163
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
PsrI GAACNNNNNNTAC 2 cut(s) 138, 170
PstI CTGCAG 1 cut(s) 105
RseI CAYNNNNRTG 2 cut(s) 36, 285
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 2 cut(s) 40, 204
Sau96I GGNCC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 255
SetI ASST 4 cut(s) 15, 165, 198, 318
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 36, 285
SmlI CTYRAG 2 cut(s) 26, 260
SmoI CTYRAG 2 cut(s) 26, 260
Sse9I AATT 2 cut(s) 188, 246
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 90, 157
TasI AATT 2 cut(s) 188, 246
TfiI GAWTC 3 cut(s) 65, 133, 211
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 3 cut(s) 17, 62, 303
VpaK11BI GGWCC 1 cut(s) 163
VspI ATTAAT 1 cut(s) 320
XapI RAATTY 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.