Rh7AG009400

DNA-directed RNA-polymerase II subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002628
Physical Location & Seq
Reverse (-)
654456 .. 656895
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG009400.1

Sequence Viewer

Length: 327 bp
ATGAATAGTGAGGTAGCCATTCTTCTTGAGCATAGATATGATCAGATGAAGCAAATGTCTGGCGATTCAACTCCACTTCCCCAAGTGTTCGAGAAATCGCTGCAGTATGTCAAGTGCTTCAGTCGGTTCACGAATCAAGGCTCTGTAAAGCAAGTTCGAGAGGTCCTCAGTAGATACCAGTTGGCCGAATTTGAGCTGGCTGTGATCGGGAATCTTTGCCCTGAAACTGTGGAGGAGGCTAAGGCAATTGTGCCCTCACTCAAGGTACGTACGTACCTGTTTATACTCTACTTTTACTTTAAAGACTGCACTAGTACATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb4

Protein Analysis

108

Amino Acids

12.49

Weight (kDa)

6.56

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb4 PF03874 1 - 90 2.1e-21 RNA polymerase Rpb4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 4 cut(s) 267, 271, 275, 316
AflIII ACRYGT 1 cut(s) 317
AgsI TTSAA 1 cut(s) 69
AhlI ACTAGT 1 cut(s) 311
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 163
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 255
BaeI ACNNNNGTAYC 4 cut(s) 257, 257, 290, 290
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 87
BclI TGATCA 1 cut(s) 40
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 47, 245
BsaAI YACGTR 2 cut(s) 269, 273
Bse1I ACTGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 1 cut(s) 87
BsgI GTGCAG 1 cut(s) 292
BshFI GGCC 1 cut(s) 185
BsiWI CGTACG 1 cut(s) 269
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 40, 204
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 105
BsrI ACTGG 1 cut(s) 178
BssMI GATC 2 cut(s) 40, 204
Bst4CI ACNGT 1 cut(s) 229
BstBAI YACGTR 2 cut(s) 269, 273
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 167, 240
BstKTI GATC 2 cut(s) 43, 207
BstMBI GATC 2 cut(s) 40, 204
BstNSI RCATGY 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 255
BstSNI TACGTA 2 cut(s) 269, 273
BstV1I GCAGC 1 cut(s) 87
BsuRI GGCC 1 cut(s) 185
Cac8I GCNNGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 4 cut(s) 266, 270, 274, 315
CviAII CATG 1 cut(s) 318
CviJI RGCY 6 cut(s) 17, 141, 185, 196, 200, 239
CviKI_1 RGCY 6 cut(s) 17, 141, 185, 196, 200, 239
CviQI GTAC 4 cut(s) 266, 270, 274, 315
DdeI CTNAG 2 cut(s) 167, 240
DpnI GATC 2 cut(s) 42, 206
DpnII GATC 2 cut(s) 40, 204
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 183
Eco105I TACGTA 2 cut(s) 269, 273
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 163
FaeI CATG 1 cut(s) 321
FaiI YATR 6 cut(s) 33, 39, 108, 284, 319, 325
FatI CATG 1 cut(s) 317
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 1 cut(s) 312
GluI GCNGC 1 cut(s) 101
HaeIII GGCC 1 cut(s) 185
Hin1II CATG 1 cut(s) 321
HinfI GANTC 3 cut(s) 65, 133, 211
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 5 cut(s) 26, 91, 130, 158, 208
Hpy8I GTNNAC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 268, 272
HpyCH4V TGCA 2 cut(s) 103, 309
HpyF3I CTNAG 2 cut(s) 167, 240
HpySE526I ACGT 2 cut(s) 268, 272
Hsp92II CATG 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 2 cut(s) 40, 204
LpnPI CCDG 5 cut(s) 45, 182, 191, 234, 290
Lsp1109I GCAGC 1 cut(s) 87
MaeI CTAG 1 cut(s) 312
MaeII ACGT 2 cut(s) 268, 272
MalI GATC 2 cut(s) 42, 206
MboI GATC 2 cut(s) 40, 204
MboII GAAGA 1 cut(s) 14
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 2 cut(s) 188, 246
MnlI CCTC 6 cut(s) 4, 154, 176, 226, 229, 265
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 36
MunI CAATTG 1 cut(s) 246
NdeII GATC 2 cut(s) 40, 204
NlaIII CATG 1 cut(s) 321
NspI RCATGY 1 cut(s) 321
PciI ACATGT 1 cut(s) 317
PfeI GAWTC 3 cut(s) 65, 133, 211
Pfl23II CGTACG 1 cut(s) 269
PkrI GCNGC 1 cut(s) 102
Ppu21I YACGTR 2 cut(s) 269, 273
PpuMI RGGWCCY 1 cut(s) 163
PscI ACATGT 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 163
PspLI CGTACG 1 cut(s) 269
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
PsrI GAACNNNNNNTAC 2 cut(s) 138, 170
PstI CTGCAG 1 cut(s) 105
RsaI GTAC 4 cut(s) 267, 271, 275, 316
RsaNI GTAC 4 cut(s) 266, 270, 274, 315
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 2 cut(s) 40, 204
Sau96I GGNCC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 255
SetI ASST 7 cut(s) 15, 165, 198, 267, 271, 275, 279
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 2 cut(s) 26, 260
SmoI CTYRAG 2 cut(s) 26, 260
SnaBI TACGTA 2 cut(s) 269, 273
SpeI ACTAGT 1 cut(s) 311
Sse9I AATT 2 cut(s) 188, 246
SspMI CTAG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 271, 275
TaqI TCGA 2 cut(s) 90, 157
TasI AATT 2 cut(s) 188, 246
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 3 cut(s) 65, 133, 211
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 17, 62
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 321
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.