pycom14g20610

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010387
Physical Location & Seq
Reverse (-)
21644251 .. 21645762
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGAACAACAAAGCCTCTGTTTCGAAAGACCTCAACGCCAAACACCTCAAGATTTTAGAAGGTCTTCTTAAGCTGCCACAAAACAGGGAATGTGCTGATTGCCGGAGCAAAGCTCCGCGATGGGCTAGTGTCAGCCTGGGAATATTTATTTGCATGCAATGCTCAGGAATTCATCGGAGTCTTGGGGTGCATATTTCAAAGGTACGGTCTACAACTTTGGATACATGGCTGCCAGAACAGGTTGCTTTTATGGAATCCATGGGTAATGAAAGGGCAAACAGTTATTGGGAAAAAGAATTGCCACCAAATATTGACAGAAGTGGGACTGAGAAATTTATTTGGGCCAAGTATGACGGAAGAATGTGGGTTGCGAAGAATGCAAAACAACCAGCTCCTGAACCAGTAGATATGAGCATCCATTCCAACAATATGGTAAAGGGTGGAGCCACATCTGGAGCTCCTAAGAAAACAAGGAGAAATTCTCTTGAGGAAGCAATGCTTTTTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

170

Amino Acids

19.01

Weight (kDa)

9.46

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 18 - 125 1.4e-40 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 209
AccII CGCG 1 cut(s) 118
AciI CCGC 1 cut(s) 116
AcsI RAATTY 3 cut(s) 168, 332, 478
AfaI GTAC 1 cut(s) 204
AflII CTTAAG 1 cut(s) 68
AgsI TTSAA 1 cut(s) 198
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 4 cut(s) 73, 113, 392, 458
AluI AGCT 4 cut(s) 73, 113, 392, 458
Alw21I GWGCWC 1 cut(s) 460
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 73, 229
ApoI RAATTY 3 cut(s) 168, 332, 478
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 342
AsuII TTCGAA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 2 cut(s) 60, 216
BccI CCATC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 1 cut(s) 214
BfaI CTAG 1 cut(s) 126
BfrI CTTAAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 2 cut(s) 74, 230
BlsI GCNGC 2 cut(s) 75, 231
Bme1390I CCNGG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 466, 498
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 2 cut(s) 32, 506
BsaJI CCNNGG 2 cut(s) 136, 258
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 2 cut(s) 164, 501
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 136, 258
BseGI GGATG 1 cut(s) 414
BseMI GCAATG 2 cut(s) 164, 501
BseMII CTCAG 2 cut(s) 177, 318
BseNI ACTGG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 60, 216
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 1 cut(s) 103
BslFI GGGAC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 460
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 1 cut(s) 116
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 2 cut(s) 176, 319
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 445
BspT104I TTCGAA 1 cut(s) 23
BspTI CTTAAG 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 164, 501
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 2 cut(s) 136, 258
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 207, 281
BstAFI CTTAAG 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 3 cut(s) 163, 327, 462
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 2 cut(s) 159, 377
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 135
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 60, 216
BstV2I GAAGAC 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 430
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 155
CaiI CAGNNNCTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 203
CviAII CATG 3 cut(s) 154, 225, 259
CviQI GTAC 1 cut(s) 203
DdeI CTNAG 3 cut(s) 163, 327, 462
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 3 cut(s) 157, 228, 262
FaiI YATR 8 cut(s) 155, 192, 226, 251, 260, 351, 410, 431
FalI AAGNNNNNCTT 3 cut(s) 51, 83, 483
FaqI GGGAC 1 cut(s) 337
FatI CATG 3 cut(s) 153, 224, 258
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 2 cut(s) 74, 230
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 2 cut(s) 74, 230
FspBI CTAG 1 cut(s) 126
GluI GCNGC 2 cut(s) 74, 230
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 1 cut(s) 344
HapII CCGG 1 cut(s) 103
Hin1II CATG 3 cut(s) 157, 228, 262
HinfI GANTC 2 cut(s) 178, 254
HpaII CCGG 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 5 cut(s) 49, 165, 395, 453, 485
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 2 cut(s) 207, 281
HpyCH4V TGCA 4 cut(s) 153, 157, 190, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 377
HpyF3I CTNAG 3 cut(s) 163, 327, 462
Hsp92II CATG 3 cut(s) 157, 228, 262
LmnI GCTCC 6 cut(s) 105, 118, 397, 443, 455, 463
Lsp1109I GCAGC 2 cut(s) 60, 216
LweI GCATC 1 cut(s) 423
MaeI CTAG 1 cut(s) 126
MboII GAAGA 3 cut(s) 56, 369, 385
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 4 cut(s) 168, 296, 332, 478
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 4 cut(s) 25, 41, 56, 481
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 1 cut(s) 69
MspCI CTTAAG 1 cut(s) 68
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 159, 377
NcoI CCATGG 1 cut(s) 258
NlaIII CATG 3 cut(s) 157, 228, 262
NlaIV GGNNCC 1 cut(s) 445
NspI RCATGY 1 cut(s) 157
NspV TTCGAA 1 cut(s) 23
PaeI GCATGC 1 cut(s) 157
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 75, 231
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SacI GAGCTC 1 cut(s) 460
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 2 cut(s) 74, 230
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 460
SetI ASST 9 cut(s) 33, 48, 64, 75, 115, 204, 243, 394, 460
SfaNI GCATC 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 23
SmlI CTYRAG 3 cut(s) 47, 68, 485
SmoI CTYRAG 3 cut(s) 47, 68, 485
SphI GCATGC 1 cut(s) 157
Sse9I AATT 4 cut(s) 168, 296, 332, 478
SsiI CCGC 1 cut(s) 116
SspI AATATT 2 cut(s) 144, 310
SspMI CTAG 1 cut(s) 126
SstI GAGCTC 1 cut(s) 460
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 207, 281
TaqI TCGA 1 cut(s) 23
TasI AATT 4 cut(s) 168, 296, 332, 478
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TseI GCWGC 2 cut(s) 73, 229
TspDTI ATGAA 3 cut(s) 17, 161, 282
TspGWI ACGGA 1 cut(s) 369
Vha464I CTTAAG 1 cut(s) 68
XapI RAATTY 3 cut(s) 168, 332, 478
XceI RCATGY 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.