pycom15g00590

Nucleoredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009764
Physical Location & Seq
Reverse (-)
335993 .. 340018
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGAACGTGAAGATCAGCCATGGTTATGAAGCTGCAAGCAACGGTGGTGGGAGTTGGGGTGGGGGTGGTGCTGCCGCCGACCCCGTTTCGAGCTCAAGAGTTTCATCGCTCCTGGCCTCCCAAGATCGTGACTACCTTCTTACTCCGACTGGAAACCAGGTAAAAGTTTCTGATCTTGATGGGAAGATAATAGGCCTATACTTCTCAGCCAATTGGTACCCGCCATGCTGGAACTTCAACGAAGTCCTAGTTGGCATCTACGAGCAGCTGAAGAACAGTGGGACCAACTTCGAGATTGTGTACGTGTCATCCGACGAAGACTCTGACGCCTTCAACATCTACCATGCATGCATGCCGTGGCTGGCGATTCCTTTCTCTGACTTGGACACCAAGAAAGCCTTGAACCGTAAGTTTGACGTGGAAAGTATTCCCAGCTTGGTTATTTTGCAACCTAATGATAACAAGGACGAGGCTACGTTGCATGATGGAGTTGAAATTATCTATCGCTATGGGGTCGTAGCCTTTCCTTTCACAACACAAAAGTTGGAAGAATTGCAAGATGAGGACAGAGCAAGGCATGAGAACCAAACTCTGACCAATTTACTAGTAAATCATGATAGAGATTATCTTTTGGGTCACCCCACGCCTAAACAGGTATCTGTTGCTTCATTAAGAGGCAAAACCATTGGACTCTACTTCTCAGCTCAATGGTGTCGTCCATGCGTTAATTTCACTCCCAAGTTGATCGATCCATCTATGAAAAAATCAAGGAGAAAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Thioredoxin_8

Protein Analysis

261

Amino Acids

29.01

Weight (kDa)

5.69

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_8 PF13905 61 - 153 1.4e-22 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 216
AciI CCGC 2 cut(s) 75, 221
AclWI GGATC 1 cut(s) 743
AcuI CTGAAG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 2 cut(s) 218, 302
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 238, 334, 403, 494
AhlI ACTAGT 1 cut(s) 604
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 2 cut(s) 111, 156
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AluBI AGCT 5 cut(s) 32, 93, 268, 435, 704
AluI AGCT 5 cut(s) 32, 93, 268, 435, 704
Alw21I GWGCWC 1 cut(s) 95
AlwI GGATC 1 cut(s) 743
AoxI GGCC 2 cut(s) 114, 193
ApeKI GCWGC 3 cut(s) 32, 71, 265
Asp700I GAANNNNTTC 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 19, 58, 277
BccI CCATC 3 cut(s) 173, 479, 760
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 2 cut(s) 113, 158
BcuI ACTAGT 1 cut(s) 604
BfaI CTAG 3 cut(s) 248, 605, 781
BisI GCNGC 4 cut(s) 33, 72, 75, 266
BlsI GCNGC 4 cut(s) 34, 73, 76, 267
Bme1390I CCNGG 2 cut(s) 113, 158
Bme18I GGWCC 1 cut(s) 282
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 218, 283
BmrFI CCNGG 2 cut(s) 113, 158
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 747
BsaAI YACGTR 1 cut(s) 304
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 19, 356
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bse1I ACTGG 1 cut(s) 154
BseBI CCWGG 2 cut(s) 113, 158
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 19, 356
BseGI GGATG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 219, 714
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 3 cut(s) 19, 58, 277
BseYI CCCAGC 1 cut(s) 431
BshFI GGCC 2 cut(s) 116, 195
BshNI GGYRCC 1 cut(s) 216
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 95
BslFI GGGAC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 2 cut(s) 116, 195
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 5 cut(s) 12, 124, 172, 744, 748
Bsp19I CCATGG 1 cut(s) 19
BspACI CCGC 2 cut(s) 75, 221
BspANI GGCC 2 cut(s) 116, 195
BspCNI CTCAG 2 cut(s) 218, 713
BspDI ATCGAT 1 cut(s) 747
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 218, 283
BspPI GGATC 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 216
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 19, 356
BssMI GATC 5 cut(s) 12, 124, 172, 744, 748
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 19
Bst2UI CCWGG 2 cut(s) 113, 158
Bst4CI ACNGT 3 cut(s) 44, 278, 407
BstACI GRCGYC 1 cut(s) 327
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 4 cut(s) 37, 349, 353, 363
BstDEI CTNAG 2 cut(s) 205, 700
BstDSI CCRYGG 2 cut(s) 19, 356
BstEII GGTNACC 1 cut(s) 635
BstF5I GGATG 1 cut(s) 308
BstKTI GATC 5 cut(s) 15, 127, 175, 747, 751
BstMBI GATC 5 cut(s) 12, 124, 172, 744, 748
BstNI CCWGG 2 cut(s) 113, 158
BstNSI RCATGY 2 cut(s) 351, 355
BstPI GGTNACC 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 111, 156
BstV1I GCAGC 3 cut(s) 19, 58, 277
BstV2I GAAGAC 1 cut(s) 324
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 2 cut(s) 116, 195
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 2 cut(s) 19, 356
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 4 cut(s) 37, 349, 353, 363
CciI TCATGA 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 282
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 335
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 217, 301
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 9 cut(s) 20, 225, 344, 348, 352, 482, 578, 614, 720
CviQI GTAC 2 cut(s) 217, 301
DdeI CTNAG 2 cut(s) 205, 700
DpnI GATC 5 cut(s) 14, 126, 174, 746, 750
DpnII GATC 5 cut(s) 12, 124, 172, 744, 748
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 19
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 290
Eco91I GGTNACC 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 93
EcoO65I GGTNACC 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 111, 156
EcoT14I CCWWGG 1 cut(s) 19
EcoT22I ATGCAT 2 cut(s) 349, 353
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 9 cut(s) 23, 228, 347, 351, 355, 485, 581, 617, 723
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 1 cut(s) 295
FatI CATG 9 cut(s) 19, 224, 343, 347, 351, 481, 577, 613, 719
FauI CCCGC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 33, 72, 75, 266
FokI GGATG 1 cut(s) 295
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 4 cut(s) 33, 72, 75, 266
FspBI CTAG 3 cut(s) 248, 605, 781
GluI GCNGC 4 cut(s) 33, 72, 75, 266
GsaI CCCAGC 1 cut(s) 435
HaeIII GGCC 2 cut(s) 116, 195
HgaI GACGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 9 cut(s) 23, 228, 347, 351, 355, 485, 581, 617, 723
HinfI GANTC 3 cut(s) 320, 367, 690
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 6 cut(s) 147, 172, 313, 325, 379, 594
Hpy188III TCNNGA 5 cut(s) 96, 128, 176, 292, 614
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 2 cut(s) 146, 340
HpyCH4III ACNGT 3 cut(s) 44, 278, 407
HpyCH4IV ACGT 4 cut(s) 6, 303, 417, 476
HpyCH4V TGCA 6 cut(s) 35, 347, 351, 448, 481, 556
HpyF3I CTNAG 2 cut(s) 205, 700
HpySE526I ACGT 4 cut(s) 6, 303, 417, 476
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 9 cut(s) 23, 228, 347, 351, 355, 485, 581, 617, 723
KpnI GGTACC 1 cut(s) 220
Kzo9I GATC 5 cut(s) 12, 124, 172, 744, 748
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 9 cut(s) 98, 125, 135, 143, 170, 214, 347, 445, 638
Lsp1109I GCAGC 3 cut(s) 19, 58, 277
LweI GCATC 1 cut(s) 264
MabI ACCWGGT 1 cut(s) 156
MaeI CTAG 3 cut(s) 248, 605, 781
MaeII ACGT 4 cut(s) 6, 303, 417, 476
MaeIII GTNAC 2 cut(s) 128, 635
MalI GATC 5 cut(s) 14, 126, 174, 746, 750
MboI GATC 5 cut(s) 12, 124, 172, 744, 748
MboII GAAGA 5 cut(s) 22, 196, 283, 329, 560
MfeI CAATTG 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 5 cut(s) 211, 495, 551, 598, 727
MlyI GAGTC 2 cut(s) 314, 684
MmeI TCCRAC 3 cut(s) 170, 336, 525
MnlI CCTC 4 cut(s) 127, 463, 556, 668
Mph1103I ATGCAT 2 cut(s) 349, 353
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 2 cut(s) 671, 726
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 113, 158
MunI CAATTG 1 cut(s) 211
MvaI CCWGG 2 cut(s) 113, 158
NcoI CCATGG 1 cut(s) 19
NdeII GATC 5 cut(s) 12, 124, 172, 744, 748
NlaIII CATG 9 cut(s) 23, 228, 347, 351, 355, 485, 581, 617, 723
NlaIV GGNNCC 2 cut(s) 218, 283
NmuCI GTSAC 2 cut(s) 128, 635
NsiI ATGCAT 2 cut(s) 349, 353
NspI RCATGY 2 cut(s) 351, 355
PaeI GCATGC 2 cut(s) 351, 355
PagI TCATGA 1 cut(s) 613
PceI AGGCCT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 4 cut(s) 34, 73, 76, 267
PleI GAGTC 2 cut(s) 314, 684
PpsI GAGTC 2 cut(s) 314, 684
Ppu21I YACGTR 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 111, 156
PspEI GGTNACC 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 2 cut(s) 111, 156
PspN4I GGNNCC 2 cut(s) 218, 283
PspPI GGNCC 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 218, 302
RsaNI GTAC 2 cut(s) 217, 301
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 2 cut(s) 671, 726
SatI GCNGC 4 cut(s) 33, 72, 75, 266
Sau3AI GATC 5 cut(s) 12, 124, 172, 744, 748
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 2 cut(s) 314, 684
ScrFI CCNGG 2 cut(s) 113, 158
SduI GDGCHC 1 cut(s) 95
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 1 cut(s) 264
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
SpeI ACTAGT 1 cut(s) 604
SphI GCATGC 2 cut(s) 351, 355
Sse9I AATT 5 cut(s) 211, 495, 551, 598, 727
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 2 cut(s) 75, 221
SspMI CTAG 3 cut(s) 248, 605, 781
SstI GAGCTC 1 cut(s) 95
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 111, 156
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 3 cut(s) 44, 278, 407
TaiI ACGT 4 cut(s) 9, 306, 420, 479
TaqI TCGA 3 cut(s) 89, 291, 747
TasI AATT 5 cut(s) 211, 495, 551, 598, 727
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 671, 726
Tru9I TTAA 2 cut(s) 671, 726
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 128, 635
TseI GCWGC 3 cut(s) 32, 71, 265
Tsp45I GTSAC 2 cut(s) 128, 635
TspDTI ATGAA 5 cut(s) 17, 42, 93, 657, 773
TspRI CASTG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 282
XceI RCATGY 2 cut(s) 351, 355
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 3 cut(s) 248, 605, 781
Zsp2I ATGCAT 2 cut(s) 349, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.