pycom15g01590

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005427
Physical Location & Seq
Reverse (-)
908984 .. 913473
4490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2475 bp
ATGATATTCTGCTCAAGAAAGCTGTTGAGGATGGTGCTTCCTTGGAGTCACTTGCTAAAGGTGCAGTGCATTTTTCTCGAAGATTTACTGTTCGTGAACTGCAAGATCGATGGTATTCTCTCCTGTATCGATCCAGTAGTTTCTGAAGATTCTTCTGCTCGCATGATTGAGTTTGAGAGTTCCACAACAACTGTTCCATTTGACAGGACAGGAAATTCAAAGGGAAAAAAATGTGAATCCGGGAAGAGAAAAGCTGAAAGTGTTCGTAGTAGTTACTATGCAATGCGTAAAAGGATTTGCAATGAGCCGTTTCATTCCATGGGCCTTAACTTTCTTGTTGCACCCAGTAATAACAACTATGATGGAAATGGAGATGAGCCTCTTTACGGAAATTGCATGAATGGAGATCCAACCTCAGCCTCTTTTGGCCTTGAGACATCAGATATGGATGCAATGCAAAATCTGATGGATGTCGGCACTGATGATACTTTTCATATGCTCCAGAACCCAGATGGGAATGATTTTCATATGGAACAAGGTAATATACATGAAGATATTATGACATGGAATGAATCTGAGGTGGATGAATTCAATCATCCGGAGGGATTGCCAGATTGCAGCCTGTTCAATGCCGATGACATAGGAATGAAACCTCTGTTTACACTTGGTCAAATTAATGGCAACGAGGGAAACATGTGCACAGAGTTTGAAGGGAACAAGGCCTTTAATTCACCTGTTTCAAACACTGTTGCATTGTTTAACAACTTGGAGTATTCATCTCCACTTCCAGCTATGCCAGTTGATGTTGATCTTAGAGAACATGATATATGCACCAGTGATTCTTTTGAACTTCATGAAAATATCGATAACAAGGCAAGAACAACAGAGAATGATGCTCATATTGGGATGGAGGTGGCCACTGAAATGCCATGCTGTGATTTCCAGAGTTCTGCAGCTCCTGTTTGTAATGAAGGTTATTTGGCAGAATTGTCCAATTCGCTTTTAAACTTTACGAATGAGGAGCTCATGCTCGTGACTGCTGATGGAAAAGATGTGATTGATAAATCTTACTATGATGGCCTGAGCTCCCTTTTGTTGAGTTCACCGAAAGATGATGTTCTTTGTGAACCGACAACCATCAAAACTGAGCCAGAAACATCTGTAGCTCCAGTTATGGACTCCATGAACCCATCTAGTACGTATCCTGGAGTGGTAGATGACAATAGAGGATCCCAAAATGCTGATGAAAATATAGTCTGCCATTCAGACACTCCAATGCTATCATCTTCAACAGCTTCAAACTATCAAAATCCTGAATTGAGGGATGGATTTATCTGCTGCACATTAAACACCGAGGACCCAGAAATCCCATGCAATGGTGATATTTTTCTACCAAATCGTTCATCAACTTCTGAAGGGAATAAGCCAATATCTTTGTCCACTAGTGGTTTCCCTGTTAATAAGAGAAATAATCACACAGGGCCGTGCTTTATGCATAAAGAGCGAAAAAATCTTGGAGAACCTCATAGTACCTCTCAGATTAAAGGATCACATTTCTTACAAGAAAAGGGTCGAAAATCTCCACTAGGTAATTTCGGGGTGAAATTTGAGCTGTCTGATTTCCCCAGTGAGGTGGCATCTAAGAATGCAGGTCATGTTGGTGAAGGTCTGGGCCAAATTGATTCAGCCAATCCAAGTACAAGTGTTCAACCTGGAATCCTGAAGGAAGGCACTAGAGAGAGTATATCGGAAAAGCATCTCAGTTATAACTCAACTGAGTTTCATGTGGAGAACCCAGATTCTGGTGTCATTAAAAAGGAGCTTGATCCATCAGCTACAATTGGGGACGATATATCATTACATATGGAAGGGATGCCCACGTATATTGCTGAACCACAATTAAATCCCGATACATCGGATCAGCTGGGGCTTTTTGAGAGCGATGATGATATACCATGCTATTCGGATATAGAGGCAATGGTACTTGATATGGATTTGGACCCAGATGACCAGGATTTGTGTTCTAGTGAGGAAGTCTCAAAATATCAGGATGAGGACGCTAAGAGGAGAATCATAAGGCTGGAGCAGTGTGCTTATTCATATTTGCAAAGAGCCATTGCTTCTCATGGAGCTTTTGCCATTTTGTATGGACGTCATTCTAAGCATTACATTAAGAAGCCCGAGGTTGTATTGGGTAGAGCAACAGACGATTCTGTTGTTGACATTGACTTGGGAAGAGACGGTCGTGGTAATAAAATCTCTCGGAAACAGGCAATGATAAATATGGATAAAGATGGATCGTTCCATTTGAAAAATCTTGGGAAGTGTTCAATCTCGGTAAATAGCAAGGAAGTAGCCCCTGGACAGAGTCTAAGCCTCAGTTCAAGTTGCTTGATTGAGATACGGGGAATGCCATTCATATTTGAGATGAACCAAACACGCGTGAAGCAGTATTTGGAAAGCATCCCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

825

Amino Acids

90.98

Weight (kDa)

4.61

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 729 - 801 4.7e-06 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1767
AatII GACGTC 1 cut(s) 2155
Acc36I ACCTGC 1 cut(s) 1640
AccB7I CCANNNNNTGG 2 cut(s) 1376, 1802
AccII CGCG 1 cut(s) 2442
AccIII TCCGGA 1 cut(s) 598
AclWI GGATC 8 cut(s) 125, 401, 1224, 1237, 1555, 1820, 1926, 2305
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 3 cut(s) 214, 587, 1604
AcuI CTGAAG 3 cut(s) 165, 1434, 1742
AcyI GRCGYC 1 cut(s) 2152
AfaI GTAC 4 cut(s) 1198, 1531, 1699, 1983
AfiI CCNNNNNNNGG 4 cut(s) 386, 1376, 1630, 1802
AflIII ACRYGT 2 cut(s) 693, 2440
AhlI ACTAGT 1 cut(s) 1442
AjnI CCWGG 4 cut(s) 1204, 1711, 2010, 2359
AjuI GAANNNNNNNTTGG 2 cut(s) 2438, 2470
Alw21I GWGCWC 3 cut(s) 701, 1026, 1088
Alw26I GTCTC 3 cut(s) 428, 2041, 2232
Alw44I GTGCAC 1 cut(s) 697
AlwI GGATC 8 cut(s) 125, 401, 1224, 1237, 1555, 1820, 1926, 2305
AlwNI CAGNNNCTG 2 cut(s) 959, 1802
Ama87I CYCGRG 1 cut(s) 2180
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 7 cut(s) 322, 427, 720, 915, 1078, 1481, 1672
ApaLI GTGCAC 1 cut(s) 697
ApeKI GCWGC 3 cut(s) 618, 953, 1338
ApoI RAATTY 3 cut(s) 214, 587, 1604
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 261
AspS9I GGNCC 5 cut(s) 322, 1357, 1481, 1672, 1999
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 5 cut(s) 723, 1095, 1391, 1612, 1673
AvaI CYCGRG 1 cut(s) 2180
AvaII GGWCC 2 cut(s) 1357, 1999
BaeGI GKGCMC 1 cut(s) 701
BalI TGGCCA 1 cut(s) 917
BamHI GGATCC 1 cut(s) 1229
BanII GRGCYC 2 cut(s) 1026, 1088
BauI CACGAG 1 cut(s) 1031
Bbv12I GWGCWC 3 cut(s) 701, 1026, 1088
BbvCI CCTCAGC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 630, 965, 1325
BceAI ACGGC 2 cut(s) 292, 1468
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 4 cut(s) 1206, 1713, 2012, 2361
BciVI GTATCC 1 cut(s) 1212
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 3 cut(s) 428, 2041, 2232
BcuI ACTAGT 1 cut(s) 1442
BfaI CTAG 5 cut(s) 1194, 1443, 1586, 1734, 2025
BfmI CTRYAG 2 cut(s) 951, 1161
BfuAI ACCTGC 1 cut(s) 1640
BfuI GTATCC 1 cut(s) 1212
BisI GCNGC 3 cut(s) 619, 954, 1339
BlsI GCNGC 3 cut(s) 620, 955, 1340
Bme1390I CCNGG 5 cut(s) 241, 1206, 1713, 2012, 2361
Bme18I GGWCC 2 cut(s) 1357, 1999
BmeT110I CYCGRG 1 cut(s) 2180
BmgT120I GGNCC 5 cut(s) 322, 1357, 1481, 1672, 1999
BmiI GGNNCC 3 cut(s) 1231, 1359, 2001
BmrFI CCNGG 5 cut(s) 241, 1206, 1713, 2012, 2361
BmrI ACTGGG 2 cut(s) 339, 1620
BmsI GCATC 5 cut(s) 439, 883, 1646, 1765, 1863
BmuI ACTGGG 2 cut(s) 339, 1620
BplI GAGNNNNNCTC 2 cut(s) 2021, 2053
BpmI CTGGAG 4 cut(s) 485, 1152, 1227, 2102
Bpu10I CCTNAGC 2 cut(s) 415, 1082
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 241
Bsa29I ATCGAT 3 cut(s) 108, 129, 864
BsaAI YACGTR 2 cut(s) 1200, 1881
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 2152
BsaJI CCNNGG 5 cut(s) 41, 318, 1353, 2181, 2359
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 4 cut(s) 386, 1376, 1630, 1802
Bse1I ACTGG 6 cut(s) 134, 345, 797, 834, 1169, 1626
Bse3DI GCAATG 7 cut(s) 288, 307, 459, 1381, 1983, 2115, 2280
Bse8I GATNNNNATC 1 cut(s) 807
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 4 cut(s) 1206, 1713, 2012, 2361
BseCI ATCGAT 3 cut(s) 108, 129, 864
BseDI CCNNGG 5 cut(s) 41, 318, 1353, 2181, 2359
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 4 cut(s) 386, 1376, 1630, 1802
BseMI GCAATG 7 cut(s) 288, 307, 459, 1381, 1983, 2115, 2280
BseMII CTCAG 8 cut(s) 429, 567, 1073, 1137, 1550, 1767, 1774, 2392
BseNI ACTGG 6 cut(s) 134, 345, 797, 834, 1169, 1626
BseRI GAGGAG 2 cut(s) 1034, 2080
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 3 cut(s) 630, 965, 1325
BseYI CCCAGC 1 cut(s) 1924
BsgI GTGCAG 2 cut(s) 83, 1324
Bsh1236I CGCG 1 cut(s) 2442
Bsh1285I CGRYCG 1 cut(s) 2245
BshFI GGCC 7 cut(s) 324, 429, 722, 917, 1080, 1483, 1674
BshVI ATCGAT 3 cut(s) 108, 129, 864
BsiEI CGRYCG 1 cut(s) 2245
BsiHKAI GWGCWC 3 cut(s) 701, 1026, 1088
BsiHKCI CYCGRG 1 cut(s) 2180
BsiSI CCGG 2 cut(s) 240, 599
BslFI GGGAC 1 cut(s) 1859
BslI CCNNNNNNNGG 4 cut(s) 386, 1376, 1630, 1802
BsmAI GTCTC 3 cut(s) 428, 2041, 2232
BsmBI CGTCTC 1 cut(s) 2232
BsmFI GGGAC 1 cut(s) 1859
BsmI GAATGC 2 cut(s) 1651, 2415
BsnI GGCC 7 cut(s) 324, 429, 722, 917, 1080, 1483, 1674
BsoBI CYCGRG 1 cut(s) 2180
Bsp1286I GDGCHC 3 cut(s) 701, 1026, 1088
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
Bsp19I CCATGG 1 cut(s) 318
BspANI GGCC 7 cut(s) 324, 429, 722, 917, 1080, 1483, 1674
BspCNI CTCAG 8 cut(s) 428, 568, 1074, 1138, 1549, 1768, 1773, 2391
BspDI ATCGAT 3 cut(s) 108, 129, 864
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 1 cut(s) 2442
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 3 cut(s) 1231, 1359, 2001
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 1640
BspPI GGATC 8 cut(s) 125, 401, 1224, 1237, 1555, 1820, 1926, 2305
BsrDI GCAATG 7 cut(s) 288, 307, 459, 1381, 1983, 2115, 2280
BsrI ACTGG 6 cut(s) 134, 345, 797, 834, 1169, 1626
BssECI CCNNGG 5 cut(s) 41, 318, 1353, 2181, 2359
BssMI GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
BssNI GRCGYC 1 cut(s) 2152
BssSI CACGAG 1 cut(s) 1031
BssT1I CCWWGG 2 cut(s) 41, 318
Bst2BI CACGAG 1 cut(s) 1031
Bst2UI CCWGG 4 cut(s) 1206, 1713, 2012, 2361
Bst4CI ACNGT 4 cut(s) 90, 193, 748, 2243
Bst6I CTCTTC 2 cut(s) 239, 2230
BstACI GRCGYC 1 cut(s) 2152
BstBAI YACGTR 2 cut(s) 1200, 1881
BstC8I GCNNGC 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 2442
BstKTI GATC 9 cut(s) 108, 133, 409, 811, 1232, 1550, 1828, 1921, 2300
BstMAI GTCTC 3 cut(s) 428, 2041, 2232
BstMBI GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
BstMCI CGRYCG 1 cut(s) 2245
BstMWI GCNNNNNNNGC 2 cut(s) 61, 1501
BstNI CCWGG 4 cut(s) 1206, 1713, 2012, 2361
BstNSI RCATGY 1 cut(s) 697
BstSCI CCNGG 5 cut(s) 239, 1204, 1711, 2010, 2359
BstSFI CTRYAG 2 cut(s) 951, 1161
BstSLI GKGCMC 1 cut(s) 701
BstSNI TACGTA 1 cut(s) 1200
BstUI CGCG 1 cut(s) 2442
BstV1I GCAGC 3 cut(s) 630, 965, 1325
BstX2I RGATCY 2 cut(s) 406, 1229
BstXI CCANNNNNNTGG 1 cut(s) 1633
BstYI RGATCY 2 cut(s) 406, 1229
Bsu15I ATCGAT 3 cut(s) 108, 129, 864
BsuI GTATCC 1 cut(s) 1212
BsuRI GGCC 7 cut(s) 324, 429, 722, 917, 1080, 1483, 1674
BsuTUI ATCGAT 3 cut(s) 108, 129, 864
BtgI CCRYGG 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 1956
BtsI GCAGTG 2 cut(s) 71, 2093
BtsIMutI CAGTG 7 cut(s) 71, 477, 744, 841, 918, 1633, 2093
BveI ACCTGC 1 cut(s) 1640
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 2 cut(s) 959, 1802
CciI TCATGA 1 cut(s) 853
Cfr13I GGNCC 5 cut(s) 322, 1357, 1481, 1672, 1999
ClaI ATCGAT 3 cut(s) 108, 129, 864
CseI GACGC 1 cut(s) 2066
Csp6I GTAC 4 cut(s) 1197, 1530, 1698, 1982
CspCI CAANNNNNGTGG 2 cut(s) 1867, 1902
CviQI GTAC 4 cut(s) 1197, 1530, 1698, 1982
DpnI GATC 9 cut(s) 107, 132, 408, 810, 1231, 1549, 1827, 1920, 2299
DpnII GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
DraI TTTAAA 1 cut(s) 1005
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 2 cut(s) 239, 2230
EarI CTCTTC 2 cut(s) 239, 2230
Ecl136II GAGCTC 2 cut(s) 1024, 1086
Eco105I TACGTA 1 cut(s) 1200
Eco130I CCWWGG 2 cut(s) 41, 318
Eco147I AGGCCT 1 cut(s) 722
Eco24I GRGCYC 2 cut(s) 1026, 1088
Eco47I GGWCC 2 cut(s) 1357, 1999
Eco53kI GAGCTC 2 cut(s) 1024, 1086
Eco57I CTGAAG 3 cut(s) 165, 1434, 1742
Eco88I CYCGRG 1 cut(s) 2180
EcoICRI GAGCTC 2 cut(s) 1024, 1086
EcoO109I RGGNCCY 1 cut(s) 1357
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 4 cut(s) 1204, 1711, 2010, 2359
EcoT14I CCWWGG 2 cut(s) 41, 318
EcoT22I ATGCAT 1 cut(s) 1497
EcoT38I GRGCYC 2 cut(s) 1026, 1088
ErhI CCWWGG 2 cut(s) 41, 318
Esp3I CGTCTC 1 cut(s) 2232
FaqI GGGAC 1 cut(s) 1859
FauNDI CATATG 3 cut(s) 495, 528, 1863
Fnu4HI GCNGC 3 cut(s) 619, 954, 1339
FriOI GRGCYC 2 cut(s) 1026, 1088
Fsp4HI GCNGC 3 cut(s) 619, 954, 1339
FspBI CTAG 5 cut(s) 1194, 1443, 1586, 1734, 2025
GluI GCNGC 3 cut(s) 619, 954, 1339
GsaI CCCAGC 1 cut(s) 1928
GsuI CTGGAG 4 cut(s) 485, 1152, 1227, 2102
HaeIII GGCC 7 cut(s) 324, 429, 722, 917, 1080, 1483, 1674
HapII CCGG 2 cut(s) 240, 599
HgaI GACGC 1 cut(s) 2066
Hin1I GRCGYC 1 cut(s) 2152
HincII GTYRAC 1 cut(s) 2221
HindII GTYRAC 1 cut(s) 2221
HpaII CCGG 2 cut(s) 240, 599
HphI GGTGA 5 cut(s) 723, 1095, 1391, 1612, 1673
Hpy166II GTNNAC 7 cut(s) 97, 660, 699, 1103, 1127, 1440, 2221
Hpy8I GTNNAC 7 cut(s) 97, 660, 699, 1103, 1127, 1440, 2221
HpyAV CCTTC 7 cut(s) 704, 965, 1409, 1658, 1717, 1721, 1862
HpyCH4III ACNGT 4 cut(s) 90, 193, 748, 2243
HpyCH4IV ACGT 3 cut(s) 1199, 1880, 2152
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 1501
HpySE526I ACGT 3 cut(s) 1199, 1880, 2152
Hsp92I GRCGYC 1 cut(s) 2152
Kpn2I TCCGGA 1 cut(s) 598
Kzo9I GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
LmnI GCTCC 8 cut(s) 504, 961, 1021, 1091, 1171, 1819, 2083, 2129
Lsp1109I GCAGC 3 cut(s) 630, 965, 1325
LweI GCATC 5 cut(s) 439, 883, 1646, 1765, 1863
MaeI CTAG 5 cut(s) 1194, 1443, 1586, 1734, 2025
MaeII ACGT 3 cut(s) 1199, 1880, 2152
MaeIII GTNAC 3 cut(s) 47, 272, 1033
MalI GATC 9 cut(s) 107, 132, 408, 810, 1231, 1549, 1827, 1920, 2299
MboI GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
MboII GAAGA 7 cut(s) 92, 144, 158, 256, 563, 1278, 2247
MfeI CAATTG 1 cut(s) 1839
MflI RGATCY 2 cut(s) 406, 1229
MhlI GDGCHC 3 cut(s) 701, 1026, 1088
MlsI TGGCCA 1 cut(s) 917
MluI ACGCGT 1 cut(s) 2440
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 3 cut(s) 55, 1172, 2377
MmeI TCCRAC 1 cut(s) 434
Mox20I TGGCCA 1 cut(s) 917
Mph1103I ATGCAT 1 cut(s) 1497
MroI TCCGGA 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 1 cut(s) 917
MslI CAYNNNNRTG 5 cut(s) 644, 923, 1031, 1274, 1659
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 1924
MspI CCGG 2 cut(s) 240, 599
MspR9I CCNGG 5 cut(s) 241, 1206, 1713, 2012, 2361
MunI CAATTG 1 cut(s) 1839
Mva1269I GAATGC 2 cut(s) 1651, 2415
MvaI CCWGG 4 cut(s) 1206, 1713, 2012, 2361
MvnI CGCG 1 cut(s) 2442
MwoI GCNNNNNNNGC 2 cut(s) 61, 1501
NciI CCSGG 1 cut(s) 241
NcoI CCATGG 1 cut(s) 318
NdeI CATATG 3 cut(s) 495, 528, 1863
NdeII GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
NlaIV GGNNCC 3 cut(s) 1231, 1359, 2001
NmuCI GTSAC 2 cut(s) 47, 1033
NsiI ATGCAT 1 cut(s) 1497
NspI RCATGY 1 cut(s) 697
PagI TCATGA 1 cut(s) 853
PceI AGGCCT 1 cut(s) 722
PciI ACATGT 1 cut(s) 693
PctI GAATGC 2 cut(s) 1651, 2415
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 9 cut(s) 149, 236, 572, 839, 1682, 1716, 1799, 2070, 2210
PflFI GACNNNGTC 1 cut(s) 2367
PflMI CCANNNNNTGG 2 cut(s) 1376, 1802
PfoI TCCNGGA 2 cut(s) 239, 1204
PkrI GCNGC 3 cut(s) 620, 955, 1340
PleI GAGTC 3 cut(s) 54, 1172, 2376
PpsI GAGTC 3 cut(s) 54, 1172, 2376
Ppu21I YACGTR 2 cut(s) 1200, 1881
PpuMI RGGWCCY 1 cut(s) 1357
PscI ACATGT 1 cut(s) 693
PshBI ATTAAT 1 cut(s) 675
PsiI TTATAA 1 cut(s) 1767
Psp124BI GAGCTC 2 cut(s) 1026, 1088
Psp5II RGGWCCY 1 cut(s) 1357
Psp6I CCWGG 4 cut(s) 1204, 1711, 2010, 2359
PspFI CCCAGC 1 cut(s) 1924
PspGI CCWGG 4 cut(s) 1204, 1711, 2010, 2359
PspN4I GGNNCC 3 cut(s) 1231, 1359, 2001
PspPI GGNCC 5 cut(s) 322, 1357, 1481, 1672, 1999
PspPPI RGGWCCY 1 cut(s) 1357
PstI CTGCAG 1 cut(s) 955
PstNI CAGNNNCTG 2 cut(s) 959, 1802
PsuI RGATCY 2 cut(s) 406, 1229
PsyI GACNNNGTC 1 cut(s) 2367
PvuII CAGCTG 1 cut(s) 1924
RsaI GTAC 4 cut(s) 1198, 1531, 1699, 1983
RsaNI GTAC 4 cut(s) 1197, 1530, 1698, 1982
RseI CAYNNNNRTG 5 cut(s) 644, 923, 1031, 1274, 1659
SacI GAGCTC 2 cut(s) 1026, 1088
SatI GCNGC 3 cut(s) 619, 954, 1339
Sau3AI GATC 9 cut(s) 105, 130, 406, 808, 1229, 1547, 1825, 1918, 2297
Sau96I GGNCC 5 cut(s) 322, 1357, 1481, 1672, 1999
SchI GAGTC 3 cut(s) 55, 1172, 2377
ScrFI CCNGG 5 cut(s) 241, 1206, 1713, 2012, 2361
SduI GDGCHC 3 cut(s) 701, 1026, 1088
SfaNI GCATC 5 cut(s) 439, 883, 1646, 1765, 1863
SfcI CTRYAG 2 cut(s) 951, 1161
SinI GGWCC 2 cut(s) 1357, 1999
SmiMI CAYNNNNRTG 5 cut(s) 644, 923, 1031, 1274, 1659
SmlI CTYRAG 2 cut(s) 13, 431
SmoI CTYRAG 2 cut(s) 13, 431
SnaBI TACGTA 1 cut(s) 1200
SpeI ACTAGT 1 cut(s) 1442
SseBI AGGCCT 1 cut(s) 722
SspMI CTAG 5 cut(s) 1194, 1443, 1586, 1734, 2025
SstI GAGCTC 2 cut(s) 1026, 1088
StuI AGGCCT 1 cut(s) 722
StyD4I CCNGG 5 cut(s) 239, 1204, 1711, 2010, 2359
StyI CCWWGG 2 cut(s) 41, 318
TaaI ACNGT 4 cut(s) 90, 193, 748, 2243
TaiI ACGT 3 cut(s) 1202, 1883, 2155
TaqI TCGA 5 cut(s) 78, 108, 129, 864, 1573
TatI WGTACW 1 cut(s) 1697
TfiI GAWTC 9 cut(s) 149, 236, 572, 839, 1682, 1716, 1799, 2070, 2210
TscAI CASTG 7 cut(s) 71, 484, 751, 841, 925, 1633, 2093
TseFI GTSAC 2 cut(s) 47, 1033
TseI GCWGC 3 cut(s) 618, 953, 1338
Tsp45I GTSAC 2 cut(s) 47, 1033
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 7 cut(s) 71, 484, 751, 841, 925, 1633, 2093
Tth111I GACNNNGTC 1 cut(s) 2367
Van91I CCANNNNNTGG 2 cut(s) 1376, 1802
VneI GTGCAC 1 cut(s) 697
VpaK11BI GGWCC 2 cut(s) 1357, 1999
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 3 cut(s) 214, 587, 1604
XceI RCATGY 1 cut(s) 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 5 cut(s) 1194, 1443, 1586, 1734, 2025
ZraI GACGTC 1 cut(s) 2153
Zsp2I ATGCAT 1 cut(s) 1497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.