pycom15g05110

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010879
Physical Location & Seq
Forward (+)
3063281 .. 3065687
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGGAGTCGTCCCGGCTTTACGAGACAAGATCACATGCAGTGAGCGAGCGAGTAGCAGAGCTAGACGGTGGAGAAATGAACAGAGTAATGCAAGCACCGTACGATGCTACCGTACGGTTCGCGCTGGCTTCGCTGGAGCGGAATCTGCTGCCGGACGCCGTCGTGAGGCGGCTGACACGGCTGCTCTTGGCGAGCCGTCTCCGGTCCGGTTACAGGCCTTCCTCCGAGCTCCAGCTGTCCGACCTCCTTCAATTCGTGCAATGTAACGTAACACTTCCCCCGCTCTCTTTGAAGGAGATGCCCATAGCCATAAGGACTGATGATCCAAAAGCTCAACACTATGAAGTTCCCACATCTTTTTTCAAGATGGTGCTTGGGAAAAATCTAAAATATAGTTGTTGCTACTTCAATGATGAGTCAAGCACATTGGAGGATGCTGAGAAAGCAACGTTGGAGCTCTACTGTGAAAGGTCACAGATAAAAGATGGTCACACTGTTCTTGACGTTGGGTGTGGCTGGGGATCTTTGTCTTTGTACATTGCGCAGAAATATAACAACTGCAAGGTTACCGGGATCTGCAACTCAACGACACAAAAAGCTTTTATAGAAGAACAATGCCGGAATCTTCAGCTGCAGAATGTGGAGATCATCGTCGGAGATATCAGCACGTTTGAAATGGAGGCTTCCTTTGACCGAATATTTTCGATTGAAATGTTTGAGCATATGAAGAACTATAAGGATCTTCTGAAGAAGATATCCGGGTGGATGAAAGACGACGGCCTTCTGTTTGTTCATCATTTCTGCCATAAAGCATTTGCTTACCACTTTGAGGATAAAAGTGAAGATGACTGGATTACTAGGTACTTCTTCAGCGGGGGTACAATGCCCTCAGCAAATCTGCTACTTTATTTCCAGGATGATGTTTCCATTGTGAATCATTGGCTCGTCAACGGGAAGCACTACGCGCAAACAAGTGAAGAGTGGCTCAAAAGAATGGATCGGAATATGGCTTCCATAAAACCGATCATGGAGTCAACTTACGGCAAGGATTCAGCCGTGAAATGGACGGTATATTGGCGAACATTCTTCATTTCCGTTGCGGAACTCTTCGGGTACAACAACGGAGAAGAATGGATGGTTGTTCATTTCCTATTCAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

389

Amino Acids

44.99

Weight (kDa)

6.04

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 111 - 345 3.6e-47 Mycolic acid cyclopropane synthetase
TRM5-TYW2_MTfase PF02475 158 - 271 3.3e-06 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 160 - 281 1.8e-16 Methyltransferase domain
Methyltransf_23 PF13489 160 - 269 1.1e-11 Methyltransferase domain
GidB PF02527 160 - 235 8.5e-06 rRNA small subunit methyltransferase G
MTS PF05175 162 - 235 6.6e-07 Methyltransferase small domain
Methyltransf_25 PF13649 166 - 260 5.3e-16 Methyltransferase domain
Methyltransf_11 PF08241 167 - 264 3.9e-15 Methyltransferase domain
Methyltransf_12 PF08242 167 - 262 6.4e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33110 AT4G33110 AT4G33120
fragaria_vesca FvH4_2g31420
malus_domestica MD15G1054300.v1.1
prunus_persica Prupe.1G407700_v2.0.a1
pyrus_communis pycom15g05110
rosa_chinensis RchiOBHm_Chr6g0311401
rosa_laevigata RLG00000010410
rosa_multiflora Rmu_sc0006915.1_g000006
rosa_roxburghii Rroxscaffold_7G00157650
rosa_rugosa Rorug06G0388500 Rorug06G0388600.1
rosa_samantha Rh6AG503000 Rh6BG513500 Rh6CG519400 Rh6DG505600
rosa_wichuraiana Rw6G043800 Rw7G029480 Rw7G029820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 543
AccBSI CCGCTC 2 cut(s) 139, 283
AccII CGCG 2 cut(s) 122, 965
AciI CCGC 5 cut(s) 139, 169, 281, 873, 1100
AclI AACGTT 1 cut(s) 449
AclWI GGATC 5 cut(s) 317, 529, 581, 747, 1005
AcuI CTGAAG 3 cut(s) 611, 767, 853
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 6 cut(s) 101, 114, 536, 863, 880, 1115
AfiI CCNNNNNNNGG 4 cut(s) 165, 213, 829, 1062
AgsI TTSAA 7 cut(s) 251, 292, 364, 409, 674, 710, 1156
AjnI CCWGG 1 cut(s) 912
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 7 cut(s) 61, 229, 235, 332, 457, 599, 631
AluI AGCT 7 cut(s) 61, 229, 235, 332, 457, 599, 631
Alw21I GWGCWC 2 cut(s) 231, 459
Alw26I GTCTC 2 cut(s) 17, 203
AlwI GGATC 5 cut(s) 317, 529, 581, 747, 1005
AoxI GGCC 2 cut(s) 215, 778
ApeKI GCWGC 3 cut(s) 148, 181, 631
Asp700I GAANNNNTTC 1 cut(s) 700
AspLEI GCGC 3 cut(s) 124, 544, 967
AspS9I GGNCC 1 cut(s) 204
AsuC2I CCSGG 3 cut(s) 13, 571, 760
AvaII GGWCC 1 cut(s) 204
BanII GRGCYC 2 cut(s) 231, 459
Bbv12I GWGCWC 2 cut(s) 231, 459
BbvCI CCTCAGC 1 cut(s) 889
BbvI GCAGC 3 cut(s) 135, 168, 618
BccI CCATC 3 cut(s) 361, 479, 1129
BceAI ACGGC 6 cut(s) 143, 180, 194, 793, 1040, 1057
BciT130I CCWGG 1 cut(s) 914
BcnI CCSGG 3 cut(s) 13, 571, 760
BcoDI GTCTC 2 cut(s) 17, 203
BfaI CTAG 2 cut(s) 62, 858
BfmI CTRYAG 1 cut(s) 632
BisI GCNGC 4 cut(s) 149, 170, 182, 632
BlsI GCNGC 4 cut(s) 150, 171, 183, 633
Bme1390I CCNGG 4 cut(s) 13, 571, 760, 914
Bme18I GGWCC 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 204
BmrFI CCNGG 4 cut(s) 13, 571, 760, 914
BmsI GCATC 3 cut(s) 94, 288, 424
BpmI CTGGAG 2 cut(s) 155, 215
Bpu10I CCTNAGC 1 cut(s) 889
BpuMI CCSGG 3 cut(s) 13, 571, 760
BsaHI GRCGYC 1 cut(s) 156
BsaWI WCCGGW 2 cut(s) 201, 206
Bsc4I CCNNNNNNNGG 4 cut(s) 165, 213, 829, 1062
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 2 cut(s) 266, 537
BseBI CCWGG 1 cut(s) 914
BseGI GGATG 4 cut(s) 439, 771, 922, 1140
BseLI CCNNNNNNNGG 4 cut(s) 165, 213, 829, 1062
BseMI GCAATG 2 cut(s) 266, 537
BseMII CTCAG 2 cut(s) 429, 903
BseNI ACTGG 1 cut(s) 854
BseXI GCAGC 3 cut(s) 135, 168, 618
BseYI CCCAGC 1 cut(s) 516
Bsh1236I CGCG 2 cut(s) 122, 965
BshFI GGCC 2 cut(s) 217, 780
BsiHKAI GWGCWC 2 cut(s) 231, 459
BsiSI CCGG 7 cut(s) 13, 152, 202, 207, 570, 619, 759
BsiWI CGTACG 2 cut(s) 99, 112
BslI CCNNNNNNNGG 4 cut(s) 165, 213, 829, 1062
BsmAI GTCTC 2 cut(s) 17, 203
BsmBI CGTCTC 1 cut(s) 203
BsnI GGCC 2 cut(s) 217, 780
Bsp1286I GDGCHC 2 cut(s) 231, 459
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
BspACI CCGC 5 cut(s) 139, 169, 281, 873, 1100
BspANI GGCC 2 cut(s) 217, 780
BspCNI CTCAG 2 cut(s) 430, 902
BspFNI CGCG 2 cut(s) 122, 965
BspMAI CTGCAG 1 cut(s) 636
BspPI GGATC 5 cut(s) 317, 529, 581, 747, 1005
BsrBI CCGCTC 2 cut(s) 139, 283
BsrDI GCAATG 2 cut(s) 266, 537
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 1 cut(s) 854
BssMI GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
BssNI GRCGYC 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 7 cut(s) 68, 99, 112, 117, 464, 496, 1069
Bst6I CTCTTC 2 cut(s) 972, 1112
BstACI GRCGYC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 534
BstC8I GCNNGC 4 cut(s) 47, 93, 126, 193
BstDEI CTNAG 2 cut(s) 438, 889
BstEII GGTNACC 1 cut(s) 565
BstF5I GGATG 4 cut(s) 439, 771, 922, 1140
BstFNI CGCG 2 cut(s) 122, 965
BstHHI GCGC 3 cut(s) 124, 544, 967
BstKTI GATC 8 cut(s) 32, 325, 524, 576, 648, 742, 1000, 1026
BstMAI GTCTC 2 cut(s) 17, 203
BstMBI GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
BstMWI GCNNNNNNNGC 5 cut(s) 130, 145, 178, 443, 964
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 565
BstSCI CCNGG 4 cut(s) 11, 569, 758, 912
BstSFI CTRYAG 1 cut(s) 632
BstUI CGCG 2 cut(s) 122, 965
BstV1I GCAGC 3 cut(s) 135, 168, 618
BstX2I RGATCY 3 cut(s) 521, 573, 739
BstYI RGATCY 3 cut(s) 521, 573, 739
BsuRI GGCC 2 cut(s) 217, 780
BtsCI GGATG 4 cut(s) 439, 771, 922, 1140
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 492
Cac8I GCNNGC 4 cut(s) 47, 93, 126, 193
CfoI GCGC 3 cut(s) 124, 544, 967
Cfr13I GGNCC 1 cut(s) 204
CpoI CGGWCCG 1 cut(s) 204
CseI GACGC 1 cut(s) 164
Csp6I GTAC 6 cut(s) 100, 113, 535, 862, 879, 1114
CspI CGGWCCG 1 cut(s) 204
CviAII CATG 2 cut(s) 35, 1027
CviQI GTAC 6 cut(s) 100, 113, 535, 862, 879, 1114
DdeI CTNAG 2 cut(s) 438, 889
DpnI GATC 8 cut(s) 31, 324, 523, 575, 647, 741, 999, 1025
DpnII GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
Eam1104I CTCTTC 2 cut(s) 972, 1112
EarI CTCTTC 2 cut(s) 972, 1112
Ecl136II GAGCTC 2 cut(s) 229, 457
Eco147I AGGCCT 1 cut(s) 217
Eco24I GRGCYC 2 cut(s) 231, 459
Eco32I GATATC 2 cut(s) 661, 756
Eco47I GGWCC 1 cut(s) 204
Eco53kI GAGCTC 2 cut(s) 229, 457
Eco57I CTGAAG 3 cut(s) 611, 767, 853
Eco91I GGTNACC 1 cut(s) 565
EcoICRI GAGCTC 2 cut(s) 229, 457
EcoO65I GGTNACC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 912
EcoRV GATATC 2 cut(s) 661, 756
EcoT38I GRGCYC 2 cut(s) 231, 459
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 2 cut(s) 38, 1030
FatI CATG 2 cut(s) 34, 1026
FauI CCCGC 2 cut(s) 288, 866
FauNDI CATATG 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 149, 170, 182, 632
FokI GGATG 4 cut(s) 446, 778, 929, 1147
FriOI GRGCYC 2 cut(s) 231, 459
Fsp4HI GCNGC 4 cut(s) 149, 170, 182, 632
FspBI CTAG 2 cut(s) 62, 858
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 3 cut(s) 123, 543, 966
GluI GCNGC 4 cut(s) 149, 170, 182, 632
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 2 cut(s) 155, 215
HaeIII GGCC 2 cut(s) 217, 780
HapII CCGG 7 cut(s) 13, 152, 202, 207, 570, 619, 759
HgaI GACGC 1 cut(s) 164
HhaI GCGC 3 cut(s) 124, 544, 967
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 2 cut(s) 38, 1030
Hin6I GCGC 3 cut(s) 122, 542, 965
HinP1I GCGC 3 cut(s) 122, 542, 965
HincII GTYRAC 2 cut(s) 949, 1035
HindII GTYRAC 2 cut(s) 949, 1035
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 7 cut(s) 5, 142, 416, 622, 934, 1031, 1049
HpaII CCGG 7 cut(s) 13, 152, 202, 207, 570, 619, 759
Hpy166II GTNNAC 2 cut(s) 949, 1035
Hpy188I TCNGA 5 cut(s) 226, 241, 656, 747, 1002
Hpy188III TCNNGA 4 cut(s) 163, 364, 500, 1156
Hpy8I GTNNAC 2 cut(s) 949, 1035
Hpy99I CGWCG 3 cut(s) 164, 656, 779
HpyAV CCTTC 4 cut(s) 228, 257, 286, 791
HpyCH4III ACNGT 7 cut(s) 68, 99, 112, 117, 464, 496, 1069
HpyCH4IV ACGT 4 cut(s) 267, 449, 504, 668
HpyCH4V TGCA 6 cut(s) 38, 91, 259, 561, 579, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 145, 178, 443, 964
HpyF3I CTNAG 2 cut(s) 438, 889
HpySE526I ACGT 4 cut(s) 267, 449, 504, 668
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 2 cut(s) 38, 1030
HspAI GCGC 3 cut(s) 122, 542, 965
Kzo9I GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
LmnI GCTCC 3 cut(s) 136, 234, 454
Lsp1109I GCAGC 3 cut(s) 135, 168, 618
LweI GCATC 3 cut(s) 94, 288, 424
MaeI CTAG 2 cut(s) 62, 858
MaeII ACGT 4 cut(s) 267, 449, 504, 668
MaeIII GTNAC 6 cut(s) 209, 263, 268, 471, 488, 565
MalI GATC 8 cut(s) 31, 324, 523, 575, 647, 741, 999, 1025
MbiI CCGCTC 2 cut(s) 139, 283
MboI GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
MflI RGATCY 3 cut(s) 521, 573, 739
MhlI GDGCHC 2 cut(s) 231, 459
MluCI AATT 1 cut(s) 251
MlyI GAGTC 3 cut(s) 14, 425, 1040
MmeI TCCRAC 3 cut(s) 264, 432, 634
MnlI CCTC 7 cut(s) 159, 232, 254, 424, 673, 823, 898
MroXI GAANNNNTTC 1 cut(s) 700
MspA1I CMGCKG 3 cut(s) 235, 631, 873
MspI CCGG 7 cut(s) 13, 152, 202, 207, 570, 619, 759
MspR9I CCNGG 4 cut(s) 13, 571, 760, 914
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 2 cut(s) 122, 965
MwoI GCNNNNNNNGC 5 cut(s) 130, 145, 178, 443, 964
NciI CCSGG 3 cut(s) 13, 571, 760
NdeI CATATG 1 cut(s) 723
NdeII GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
NlaIII CATG 2 cut(s) 38, 1030
NmuCI GTSAC 2 cut(s) 471, 488
NsbI TGCGCA 1 cut(s) 543
NspI RCATGY 1 cut(s) 38
PceI AGGCCT 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 4 cut(s) 142, 622, 934, 1049
Pfl23II CGTACG 2 cut(s) 99, 112
PflFI GACNNNGTC 1 cut(s) 158
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 4 cut(s) 150, 171, 183, 633
PleI GAGTC 3 cut(s) 13, 424, 1039
PpsI GAGTC 3 cut(s) 13, 424, 1039
Psp124BI GAGCTC 2 cut(s) 231, 459
Psp1406I AACGTT 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 912
PspEI GGTNACC 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 516
PspGI CCWGG 1 cut(s) 912
PspLI CGTACG 2 cut(s) 99, 112
PspPI GGNCC 1 cut(s) 204
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 3 cut(s) 521, 573, 739
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 2 cut(s) 235, 631
RsaI GTAC 6 cut(s) 101, 114, 536, 863, 880, 1115
RsaNI GTAC 6 cut(s) 100, 113, 535, 862, 879, 1114
Rsr2I CGGWCCG 1 cut(s) 204
RsrII CGGWCCG 1 cut(s) 204
SacI GAGCTC 2 cut(s) 231, 459
SatI GCNGC 4 cut(s) 149, 170, 182, 632
Sau3AI GATC 8 cut(s) 29, 322, 521, 573, 645, 739, 997, 1023
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 3 cut(s) 14, 425, 1040
ScrFI CCNGG 4 cut(s) 13, 571, 760, 914
SduI GDGCHC 2 cut(s) 231, 459
SfaNI GCATC 3 cut(s) 94, 288, 424
SfcI CTRYAG 1 cut(s) 632
SinI GGWCC 1 cut(s) 204
Sse9I AATT 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 5 cut(s) 139, 169, 281, 873, 1100
SspI AATATT 1 cut(s) 699
SspMI CTAG 2 cut(s) 62, 858
SstI GAGCTC 2 cut(s) 231, 459
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 4 cut(s) 11, 569, 758, 912
TaaI ACNGT 7 cut(s) 68, 99, 112, 117, 464, 496, 1069
TaiI ACGT 4 cut(s) 270, 452, 507, 671
TaqI TCGA 1 cut(s) 704
TaqII GACCGA 1 cut(s) 708
TasI AATT 1 cut(s) 251
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 4 cut(s) 142, 622, 934, 1049
TscAI CASTG 2 cut(s) 45, 499
TseFI GTSAC 2 cut(s) 471, 488
TseI GCWGC 3 cut(s) 148, 181, 631
Tsp45I GTSAC 2 cut(s) 471, 488
TspDTI ATGAA 7 cut(s) 92, 357, 740, 782, 782, 1078, 1133
TspGWI ACGGA 2 cut(s) 1084, 1137
TspRI CASTG 2 cut(s) 45, 499
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 204
XceI RCATGY 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 2 cut(s) 62, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.