pycom15g05210
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015028
Physical Location & Seq
Reverse (-)
3112891 .. 3113703
813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGCTGGAGTACTACTCCGATTTGCAGGAGTGCTACTCGGAGGTGGAAGAATCCGAAACGATGAAGCGGAGAAAATCAAGCAGCGCCGCCTGTGCGGCGGTGGTGACGGCGGCGGTGTCGGCGAAAGCTGAAGGAGTCGATCAAGTTAAGCCGGGAGTCGGGTTGGGTCACCATCGGCGGCTGTGGGTCAAGGACAGGTCGAAAGCGTGGTGGGACGAATGCAACCAACCGGATTTTCCGGAATCGGAATTCAAAAAGGCGTTTCGGATGGGGAAATCCACGTTCGATTTAATATGCGAGGAGCTCAACTCCGCCATCGCGAAGGAGGACACGACGCTGCGGAACGCGATTCCGGTGCGACAGAGAGTGGCGGTGTGCCTTTGGCGGCTGGCCACCGGCGACCCGCTCCGCCTCGTGTCGAAACGATTCGGACTCGGCATCTCGACCTGCCACAAGCTCGTCCTTGAGGTCTGCTCCGCGATCCGGACGGTGCTGATGCCCAAGTACCTGCAGTGGCCGGAGGAGAGCGCGGCGAGGAAAATTAAGGACGAGTTCGAGGGTATTTCGGGAATTCCGAACGTTGCTGGGTCCATGTACACGTCGCATATACCCATCATCGCACCGAAAGTCAGCGTTGCTGCGTATTTCAATAAACGGCACACGGAGAGGAACCAGAAGACTTCGTACTCGATTACTGTGCAGGGTGTGGTGGACCCCAGGGGCGTTTTTACCGATGTGTGCATCGGATGGCCAGGATCAATGCCGGATCGATCAGGTGCTGGAGAAATCAGCGTTAAATCAGAGGGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

271

Amino Acids

29.99

Weight (kDa)

8.56

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 81 - 161 3.3e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 199 - 258 1.3e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 455, 516
AccBSI CCGCTC 1 cut(s) 406
AccII CGCG 4 cut(s) 320, 347, 479, 530
AccIII TCCGGA 2 cut(s) 238, 483
AclI AACGTT 1 cut(s) 579
AclWI GGATC 3 cut(s) 475, 763, 774
AcoI YGGCCR 3 cut(s) 390, 515, 749
AcsI RAATTY 2 cut(s) 248, 570
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 4 cut(s) 11, 506, 596, 686
AfiI CCNNNNNNNGG 2 cut(s) 158, 483
AflIII ACRYGT 1 cut(s) 597
AgsI TTSAA 2 cut(s) 253, 649
AjiI CACGTC 1 cut(s) 600
AjnI CCWGG 2 cut(s) 716, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 219, 251
AluBI AGCT 3 cut(s) 128, 304, 457
AluI AGCT 3 cut(s) 128, 304, 457
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 3 cut(s) 475, 763, 774
AlwNI CAGNNNCTG 1 cut(s) 779
Aor13HI TCCGGA 2 cut(s) 238, 483
AoxI GGCC 4 cut(s) 390, 515, 749, 805
ApeKI GCWGC 3 cut(s) 81, 337, 638
ApoI RAATTY 2 cut(s) 248, 570
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 2 cut(s) 86, 530
AspS9I GGNCC 3 cut(s) 588, 712, 805
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 115, 161
AvaII GGWCC 2 cut(s) 588, 712
BalI TGGCCA 2 cut(s) 392, 751
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 668, 700
BauI CACGAG 1 cut(s) 413
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 93, 324, 625
BccI CCATC 5 cut(s) 180, 262, 323, 620, 741
BceAI ACGGC 2 cut(s) 123, 671
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 679, 713
BciT130I CCWGG 2 cut(s) 718, 753
BcnI CCSGG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 509
BfoI RGCGCY 1 cut(s) 87
BfuAI ACCTGC 2 cut(s) 455, 516
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 9 cut(s) 82, 87, 96, 111, 179, 338, 386, 531, 639
BlsI GCNGC 9 cut(s) 83, 88, 97, 112, 180, 339, 387, 532, 640
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 3 cut(s) 153, 718, 753
Bme18I GGWCC 2 cut(s) 588, 712
BmgBI CACGTC 1 cut(s) 600
BmgT120I GGNCC 3 cut(s) 588, 712, 805
BmiI GGNNCC 3 cut(s) 589, 671, 714
BmrFI CCNGG 3 cut(s) 153, 718, 753
BmsI GCATC 3 cut(s) 447, 486, 750
BpiI GAAGAC 1 cut(s) 683
BplI GAGNNNNNCTC 7 cut(s) 31, 20, 52, 293, 325, 458, 490
BpmI CTGGAG 2 cut(s) 26, 801
BpuEI CTTGAG 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 716, 717
BsaWI WCCGGW 4 cut(s) 229, 238, 352, 483
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 483
Bse118I RCCGGY 1 cut(s) 395
BseAI TCCGGA 2 cut(s) 238, 483
BseBI CCWGG 2 cut(s) 718, 753
BseCI ATCGAT 1 cut(s) 769
BseDI CCNNGG 2 cut(s) 716, 717
BseGI GGATG 2 cut(s) 273, 752
BseLI CCNNNNNNNGG 2 cut(s) 158, 483
BseRI GAGGAG 2 cut(s) 314, 536
BseXI GCAGC 3 cut(s) 93, 324, 625
BseYI CCCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 719
Bsh1236I CGCG 4 cut(s) 320, 347, 479, 530
BshFI GGCC 4 cut(s) 392, 517, 751, 807
BshVI ATCGAT 1 cut(s) 769
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 8 cut(s) 152, 230, 239, 353, 396, 484, 518, 764
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 158, 483
BsmFI GGGAC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 4 cut(s) 392, 517, 751, 807
Bsp1286I GDGCHC 1 cut(s) 306
Bsp13I TCCGGA 2 cut(s) 238, 483
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 139, 480, 755, 766, 770
Bsp68I TCGCGA 1 cut(s) 320
BspANI GGCC 4 cut(s) 392, 517, 751, 807
BspDI ATCGAT 1 cut(s) 769
BspEI TCCGGA 2 cut(s) 238, 483
BspFNI CGCG 4 cut(s) 320, 347, 479, 530
BspLI GGNNCC 3 cut(s) 589, 671, 714
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 2 cut(s) 455, 516
BspPI GGATC 3 cut(s) 475, 763, 774
BsrBI CCGCTC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 716, 717
BssMI GATC 5 cut(s) 139, 480, 755, 766, 770
BssSI CACGAG 1 cut(s) 413
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 2 cut(s) 718, 753
Bst4CI ACNGT 2 cut(s) 490, 697
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 390
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 2 cut(s) 273, 752
BstFNI CGCG 4 cut(s) 320, 347, 479, 530
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 530
BstKTI GATC 5 cut(s) 142, 483, 758, 769, 773
BstMBI GATC 5 cut(s) 139, 480, 755, 766, 770
BstMWI GCNNNNNNNGC 3 cut(s) 92, 95, 119
BstNI CCWGG 2 cut(s) 718, 753
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 151, 716, 751
BstSFI CTRYAG 1 cut(s) 509
BstUI CGCG 4 cut(s) 320, 347, 479, 530
BstV1I GCAGC 3 cut(s) 93, 324, 625
BstV2I GAAGAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 769
BsuRI GGCC 4 cut(s) 392, 517, 751, 807
BsuTUI ATCGAT 1 cut(s) 769
BtgZI GCGATG 2 cut(s) 301, 601
BtrI CACGTC 1 cut(s) 600
BtsCI GGATG 2 cut(s) 273, 752
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 1 cut(s) 518
BtuMI TCGCGA 1 cut(s) 320
BveI ACCTGC 2 cut(s) 455, 516
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 2 cut(s) 86, 530
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 3 cut(s) 588, 712, 805
ClaI ATCGAT 1 cut(s) 769
CseI GACGC 1 cut(s) 343
Csp6I GTAC 4 cut(s) 10, 505, 595, 685
CviAII CATG 1 cut(s) 592
CviQI GTAC 4 cut(s) 10, 505, 595, 685
DpnI GATC 5 cut(s) 141, 482, 757, 768, 772
DpnII GATC 5 cut(s) 139, 480, 755, 766, 770
EaeI YGGCCR 3 cut(s) 390, 515, 749
EciI GGCGGA 2 cut(s) 301, 398
Ecl136II GAGCTC 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 588, 712
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 150
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 304
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 2 cut(s) 248, 570
EcoRII CCWGG 2 cut(s) 716, 751
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 1 cut(s) 595
FaiI YATR 4 cut(s) 295, 593, 606, 608
FaqI GGGAC 1 cut(s) 227
FatI CATG 1 cut(s) 591
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 9 cut(s) 82, 87, 96, 111, 179, 338, 386, 531, 639
FokI GGATG 2 cut(s) 280, 759
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 9 cut(s) 82, 87, 96, 111, 179, 338, 386, 531, 639
GlaI GCGC 2 cut(s) 85, 529
GluI GCNGC 9 cut(s) 82, 87, 96, 111, 179, 338, 386, 531, 639
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 2 cut(s) 26, 801
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 4 cut(s) 392, 517, 751, 807
HapII CCGG 8 cut(s) 152, 230, 239, 353, 396, 484, 518, 764
HgaI GACGC 1 cut(s) 343
HhaI GCGC 2 cut(s) 86, 530
Hin1II CATG 1 cut(s) 595
Hin6I GCGC 2 cut(s) 84, 528
HinP1I GCGC 2 cut(s) 84, 528
HinfI GANTC 7 cut(s) 50, 135, 156, 242, 349, 426, 432
HpaII CCGG 8 cut(s) 152, 230, 239, 353, 396, 484, 518, 764
HphI GGTGA 2 cut(s) 115, 161
Hpy166II GTNNAC 2 cut(s) 597, 712
Hpy188I TCNGA 9 cut(s) 19, 40, 55, 247, 267, 431, 576, 746, 802
Hpy188III TCNNGA 5 cut(s) 239, 319, 442, 484, 567
Hpy8I GTNNAC 2 cut(s) 597, 712
Hpy99I CGWCG 2 cut(s) 337, 604
HpyAV CCTTC 2 cut(s) 125, 316
HpyCH4III ACNGT 2 cut(s) 490, 697
HpyCH4IV ACGT 3 cut(s) 281, 579, 599
HpyCH4V TGCA 5 cut(s) 25, 222, 511, 700, 741
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 95, 119
HpySE526I ACGT 3 cut(s) 281, 579, 599
Hsp92II CATG 1 cut(s) 595
HspAI GCGC 2 cut(s) 84, 528
Kpn2I TCCGGA 2 cut(s) 238, 483
Kzo9I GATC 5 cut(s) 139, 480, 755, 766, 770
LmnI GCTCC 3 cut(s) 301, 411, 479
Lsp1109I GCAGC 3 cut(s) 93, 324, 625
LweI GCATC 3 cut(s) 447, 486, 750
MaeII ACGT 3 cut(s) 281, 579, 599
MaeIII GTNAC 2 cut(s) 103, 167
MalI GATC 5 cut(s) 141, 482, 757, 768, 772
MbiI CCGCTC 1 cut(s) 406
MboI GATC 5 cut(s) 139, 480, 755, 766, 770
MboII GAAGA 2 cut(s) 59, 688
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 2 cut(s) 392, 751
MluCI AATT 3 cut(s) 248, 540, 570
MluNI TGGCCA 2 cut(s) 392, 751
MlyI GAGTC 3 cut(s) 144, 165, 426
Mox20I TGGCCA 2 cut(s) 392, 751
MroI TCCGGA 2 cut(s) 238, 483
MroXI GAANNNNTTC 1 cut(s) 425
MscI TGGCCA 2 cut(s) 392, 751
MseI TTAA 4 cut(s) 147, 290, 543, 795
Msp20I TGGCCA 2 cut(s) 392, 751
MspI CCGG 8 cut(s) 152, 230, 239, 353, 396, 484, 518, 764
MspR9I CCNGG 3 cut(s) 153, 718, 753
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 2 cut(s) 718, 753
MvnI CGCG 4 cut(s) 320, 347, 479, 530
MwoI GCNNNNNNNGC 3 cut(s) 92, 95, 119
NciI CCSGG 1 cut(s) 153
NdeII GATC 5 cut(s) 139, 480, 755, 766, 770
NlaIII CATG 1 cut(s) 595
NlaIV GGNNCC 3 cut(s) 589, 671, 714
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 2 cut(s) 103, 167
NruI TCGCGA 1 cut(s) 320
PasI CCCWGGG 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 572
PctI GAATGC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 4 cut(s) 50, 242, 349, 426
PkrI GCNGC 9 cut(s) 83, 88, 97, 112, 180, 339, 387, 532, 640
PleI GAGTC 3 cut(s) 143, 164, 426
PpsI GAGTC 3 cut(s) 143, 164, 426
Psp124BI GAGCTC 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 716, 751
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 2 cut(s) 716, 751
PspN4I GGNNCC 3 cut(s) 589, 671, 714
PspPI GGNCC 3 cut(s) 588, 712, 805
PstI CTGCAG 1 cut(s) 513
PstNI CAGNNNCTG 1 cut(s) 779
RruI TCGCGA 1 cut(s) 320
RsaI GTAC 4 cut(s) 11, 506, 596, 686
RsaNI GTAC 4 cut(s) 10, 505, 595, 685
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 4 cut(s) 147, 290, 543, 795
SatI GCNGC 9 cut(s) 82, 87, 96, 111, 179, 338, 386, 531, 639
Sau3AI GATC 5 cut(s) 139, 480, 755, 766, 770
Sau96I GGNCC 3 cut(s) 588, 712, 805
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 3 cut(s) 144, 165, 426
ScrFI CCNGG 3 cut(s) 153, 718, 753
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 3 cut(s) 447, 486, 750
SfcI CTRYAG 1 cut(s) 509
SgrAI CRCCGGYG 1 cut(s) 395
SinI GGWCC 2 cut(s) 588, 712
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 3 cut(s) 248, 540, 570
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 3 cut(s) 151, 716, 751
TaaI ACNGT 2 cut(s) 490, 697
TaiI ACGT 3 cut(s) 284, 582, 602
TaqI TCGA 8 cut(s) 138, 200, 285, 419, 443, 555, 689, 769
TasI AATT 3 cut(s) 248, 540, 570
TatI WGTACW 2 cut(s) 9, 594
TauI GCSGC 6 cut(s) 89, 98, 113, 181, 388, 533
TfiI GAWTC 4 cut(s) 50, 242, 349, 426
Tru1I TTAA 4 cut(s) 147, 290, 543, 795
Tru9I TTAA 4 cut(s) 147, 290, 543, 795
TscAI CASTG 1 cut(s) 518
TseFI GTSAC 2 cut(s) 103, 167
TseI GCWGC 3 cut(s) 81, 337, 638
Tsp45I GTSAC 2 cut(s) 103, 167
TspDTI ATGAA 1 cut(s) 77
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 588, 712
XapI RAATTY 2 cut(s) 248, 570
XmnI GAANNNNTTC 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.