pycom15g05930

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006231
Physical Location & Seq
Forward (+)
3586970 .. 3592654
5685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3327 bp
ATGAGGAGGCTCATAGCCTGGTGCTTCAGAAACCGAGTCTCCGACAGCAGCAACCATCAGAAGCAGAAGCATCCAGAGGGATCCAAAGAAGCCATGGACGAGAAGAACCAACACACCAACAAGAATGCCGCCGACAATGCGGAGTTCTCGAGGCAAAATGTTGCTGATTCAAGTAGCAGTCGCGGTCTAAATATCGTACTGAACCATGATTTCTCTGGAGGGCTGCATTCTTGGTACCCCAATCACTGTAATGGCTTCGTAGTGGATTCAGCAGGAAGTTATGCGGTTGTCACGAATCGCCAACAATGCTGGCAGGGCTTGGAGCAAGAAATTACTCGGAGAATTTCCCCTGGCAACACTTATTCAGTTTCAGCTCGTGTGGGAGTCTCAGGGACTCTTCAGGGATCTGCTGATGTCCTGGCAACTTTGAAACTGGAAAGTCGAGGTTCAGCGACTAGCTATATGAGAATTGGAGGAAGTTCGGTGTCAAATGGGAAGTGGGAAAGCTTGGATGGAAAATTTTCGCTGTCGACAATACCTGATCGGGTTGTGTTTTATTTGGAAGGGCCTCCTGCTGGAGTTGACCTTCATATCAAATCAGTTGTGATCTCTTGTTCTGAAGGCCAGTCTGAGAACCAAAATTTGGCCAATTCAAGTAGCAGTAATGCTACAAATATCATAGTGAACCATGATTTCTCTGGAGGACTGCATTCTTGGCACCCGAATTGCTGCAATGGCTTTGTAGCTTCTGCTGATTCCGGTCACCCAGAAGTAAAAGCAGGCAATTATGCCGTTGTTACAAATCGTAAGGAAAGTTGGCAAGGTTTAGAGCAAGATATCACACGGAGAATTTCCCCAGGCTCCACATATTTGGTTTCAGCATGTGTTGGAGCCTGCGGATCTCTTCAGGGCTCCGCTGATGTCCTGGCAACTTTGAAGCTGGAATACAGAGGTTCAGCGACCAACTATTTGAAAGTTGGAAGATGTTCTGTATTGAAGGGAAGGTGGGGAAATTTAGATGGGAAGTTTTCTCTGTCGACGATGCCTAATAGGGTTGTATTTTATTTGGAAGGTCCATCACCTGGAGTTGACCTTCTAATAAAATCAGTTTTGATCTGTTCCTCCAGTCCAAATGAATGTCAGAGTGGAAGCACTGGGAATTTCAATGATGGAGAAGAGAATATAATCCTAAACCCAAACTTTGAGGATGCTCTGAATAATTGGTCTGGAAGAGGCTGCAAAATTGTTTTGCATGACTCGATGGGAGATGGGAAAATCGTCCCTCAATCTGGAAAGGTTTTTGCAGCTGCAACAGAACGCACACAGAGCTGGAATGGCATCCAGCAGGACATCACTGGAAGAGTGCAGCGAAAGCTAGCTTATGAGGCGACTGCTGTTGTTAGGATATTTGGCAACAATGTCACTACTGCTGTTGTCCGGGCTACATTATGGGTCCAGTCACCAAATCAGCGTGAACAATATATCGGCATTGCTAATGTGCAGGCAACAGACAAGGACTGGACACAGTTGCGGGGGAAGTTCCTTTTAAATGGCTCCCCGTCAAAAGTAGTAGTTTATCTTGAAGGTCCAGCAGCGGGAACAGATATTCTTGTCAATAGTTTTGTTGTCAAGCATGCTGAGAAAGTTCCTCCTTCACCTCCACCGGTTATTGAGTTTTCAGCATTTGGAGTAAATATAATTGAGAACAGCAATTTGAGTAACGGCACAAACGGGTGGTTTCCGCTTGGTAATTGTACTTTGAGTGTCACAACTGGTTCACCGCATATTCTCCCTCCAATGGCAAGAGAGAGCCTTGGACCTCATGAACCTTTAAGCGGTCGCTACATTCTAGTAACCAAGCGCACACAAACTTGGATGGGTCCTGCTCAGATGATTGGTGACAAATTGAAACTCTTTTTGACGTATCAAGTATCTGCTTGGGTTCGGATTGGTGCTGGAGCAACTGGTCCACAGAACATCAATGTTGCACTCAGCGTGGACAACCAGTGGGTCAATGGAGGTCAAGCTGAGGCAAGTGACACAAGATGGCATGAAATCGGTGGTTCCTTCAGAGTTGAGAAGCAGCCATCAAAAGTAATGGTTTATATCCAAGGTCCTGCTGCAGGTGTAGACTTAATGGTTGCCGGTCTTCAGATATTCCCCGTTGACAGACCGGCAAGATTTAGACATCTGAAGAGGCAGACTGATAAGGTTCGTAAGTGCGATATTGTCCTGAAATTTTCAGGGTTGGACTCGAGCAGCATGCTCGGCACCTTTGTGAAAGTTAAACAAACGCAAAATAGTTTTCCAATTGGGACGTGTATAAGTAGAACAAACATTGACAACGAAGATTTCATTGATTTCTTTGTTAAAAACTTCAACTGGGCTGTGTTCGGAAATGAATTGAAGTGGTATTGGACTGAACCACAAAAAGGAAACTTCAACTACAAGGATGCTGATGAGATGGTGGACATGTGCAAGAGTCACAACATAGAAATGCGAGGGCACTGTATTTTCTGGGAAGTCATCGACACAGTGCAGCAATGGATCCGCTCGTTGAGCCAGAGTGACTTGTCAACAGCTGTTCAGAATCGACTGACAGATCTTCTCACACGCTACAAAGGGAAGTTCAGGCACTATGATGTCAACAATGAGATGCTGCACGGCTCATTCTATCAGGACAAATTGGGAAAAGATATCAGAGCCAACATGTTCAAGACTGCAAACCAGTTGGATCCATCTGCCACTCTATTTGTGAATGATTATCACGTCGAGGATGGATGCGATACTAGATCATCACCAGAAAAATACACTGATCAAATCCTTGATTTGCAGCAACAAGGTGCACCTGTTGGTGGCATTGGCATCCAAGGACATATTGATAGTCCAGTGGGTCCAATTGTTTGTTCAGCTCTTGACAAATTAGGCATTCTCGGCCTTCCAATCTGGTTCACGGAGCTTGATGTGTCATCCAGTAACGAATATGTTAGAGCAGATGATTTGGAAGTGATGCTTCGTGAAGCATTTGCCAATCCAACAGTTGAAGGTGTAATGCTATGGGGATTCTGGGAGCTGTTTATGAGTCGGGAAAATTCGCATTTAGTGAATGCAGAAGGGGATATCAACGAAGCAGGAAAACGTTTCTTGGAGCTCAAGCAAGAGTGGCTATCTCACGCGCACGGCCACATTGATGAGCAGGGAGAGTTCGGGTTTAGGGGCTTCCCAGGAACATACACTGTTGAAGTTATTACTGCCCCGAAGAAGCCTGCAAAAACATTTGTCGTTGACAAGGGCGAGTCCCCAGTGGAGGTCTCCATTGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

1109

Amino Acids

121.93

Weight (kDa)

6.34

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 64 - 191 2.9e-18 Carbohydrate binding domain
CBM_4_9 PF02018 225 - 360 4.7e-19 Carbohydrate binding domain
CBM_4_9 PF02018 394 - 531 1.6e-19 Carbohydrate binding domain
CBM_4_9 PF02018 565 - 707 8.9e-17 Carbohydrate binding domain
Glyco_hydro_10 PF00331 765 - 1022 3e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2114
Acc36I ACCTGC 1 cut(s) 2114
Acc65I GGTACC 1 cut(s) 234
AccB1I GGYRCC 3 cut(s) 234, 717, 2270
AccB7I CCANNNNNTGG 2 cut(s) 643, 1082
AccBSI CCGCTC 1 cut(s) 2553
AccI GTMKAC 3 cut(s) 530, 1037, 2130
AccII CGCG 2 cut(s) 183, 3176
AclI AACGTT 1 cut(s) 3139
AclWI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
AcoI YGGCCR 2 cut(s) 645, 3181
AcsI RAATTY 8 cut(s) 342, 518, 640, 849, 1012, 1159, 2237, 3091
AcuI CTGAAG 7 cut(s) 10, 383, 639, 890, 2053, 2135, 2213
AfaI GTAC 3 cut(s) 198, 236, 1756
AflIII ACRYGT 3 cut(s) 2318, 2472, 2709
AgeI ACCGGT 1 cut(s) 1663
AjiI CACGTC 2 cut(s) 2319, 2770
AjnI CCWGG 7 cut(s) 17, 349, 417, 856, 924, 1081, 3223
AjuI GAANNNNNNNTTGG 4 cut(s) 626, 658, 2890, 2922
AleI CACNNNNGTG 1 cut(s) 2276
Alw21I GWGCWC 2 cut(s) 2848, 3153
Alw26I GTCTC 3 cut(s) 43, 391, 3316
Alw44I GTGCAC 1 cut(s) 2844
AlwI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
Ama87I CYCGRG 2 cut(s) 148, 2254
AoxI GGCC 5 cut(s) 566, 622, 645, 2935, 3181
ApaLI GTGCAC 1 cut(s) 2844
ApoI RAATTY 8 cut(s) 342, 518, 640, 849, 1012, 1159, 2237, 3091
ArsI GACNNNNNNTTYG 2 cut(s) 952, 984
AsiGI ACCGGT 1 cut(s) 1663
Asp700I GAANNNNTTC 2 cut(s) 520, 985
Asp718I GGTACC 1 cut(s) 234
AspLEI GCGC 2 cut(s) 1863, 3178
AspS9I GGNCC 9 cut(s) 566, 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
AsuC2I CCSGG 1 cut(s) 1439
AsuHPI GGTGA 7 cut(s) 755, 1071, 1452, 1647, 1770, 1910, 2790
AsuNHI GCTAGC 1 cut(s) 1375
AvaI CYCGRG 2 cut(s) 148, 2254
AvaII GGWCC 8 cut(s) 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
BaeGI GKGCMC 2 cut(s) 2508, 2848
BalI TGGCCA 1 cut(s) 647
BamHI GGATCC 3 cut(s) 80, 2547, 2734
BanI GGYRCC 3 cut(s) 234, 717, 2270
BanII GRGCYC 2 cut(s) 914, 3153
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 2141
Bbv12I GWGCWC 2 cut(s) 2848, 3153
BbvCI CCTCAGC 1 cut(s) 2028
BceAI ACGGC 4 cut(s) 776, 1738, 2680, 3196
BcgI CGANNNNNNTGC 4 cut(s) 2245, 2248, 2279, 2282
BciT130I CCWGG 7 cut(s) 19, 351, 419, 858, 926, 1083, 3225
BclI TGATCA 1 cut(s) 2815
BcnI CCSGG 1 cut(s) 1439
BcoDI GTCTC 3 cut(s) 43, 391, 3316
BfaI CTAG 4 cut(s) 456, 1376, 1850, 2790
BfmI CTRYAG 1 cut(s) 2121
BfuAI ACCTGC 1 cut(s) 2114
BglII AGATCT 1 cut(s) 2602
Bme1390I CCNGG 8 cut(s) 19, 351, 419, 858, 926, 1083, 1439, 3225
Bme18I GGWCC 8 cut(s) 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
BmeT110I CYCGRG 2 cut(s) 148, 2254
BmgBI CACGTC 2 cut(s) 2319, 2770
BmgT120I GGNCC 9 cut(s) 566, 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
BmrFI CCNGG 8 cut(s) 19, 351, 419, 858, 926, 1083, 1439, 3225
BmrI ACTGGG 3 cut(s) 1164, 2392, 3296
BmsI GCATC 9 cut(s) 79, 1032, 1198, 1347, 2443, 2646, 2771, 2874, 3000
BmtI GCTAGC 1 cut(s) 1379
BmuI ACTGGG 3 cut(s) 1164, 2392, 3296
BpiI GAAGAC 1 cut(s) 2141
BpmI CTGGAG 6 cut(s) 237, 597, 720, 1104, 1108, 1977
Bpu10I CCTNAGC 1 cut(s) 2028
BpuEI CTTGAG 1 cut(s) 3137
BpuMI CCSGG 1 cut(s) 1439
BsaI GGTCTC 1 cut(s) 3316
BsaJI CCNNGG 7 cut(s) 93, 349, 856, 1813, 2110, 2869, 3223
BsaWI WCCGGW 2 cut(s) 758, 1663
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bse118I RCCGGY 3 cut(s) 1663, 2144, 2173
Bse3DI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BseBI CCWGG 7 cut(s) 19, 351, 419, 858, 926, 1083, 3225
BseDI CCNNGG 7 cut(s) 93, 349, 856, 1813, 2110, 2869, 3223
BseMI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BseMII CTCAG 6 cut(s) 402, 621, 1629, 1901, 2005, 2019
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 2 cut(s) 2508, 2848
BsgI GTGCAG 4 cut(s) 1385, 1520, 2558, 2645
Bsh1236I CGCG 2 cut(s) 183, 3176
Bsh1285I CGRYCG 1 cut(s) 1840
BshFI GGCC 5 cut(s) 568, 624, 647, 2937, 3183
BshNI GGYRCC 3 cut(s) 234, 717, 2270
BshTI ACCGGT 1 cut(s) 1663
BsiEI CGRYCG 1 cut(s) 1840
BsiHKAI GWGCWC 2 cut(s) 2848, 3153
BsiHKCI CYCGRG 2 cut(s) 148, 2254
BsiSI CCGG 5 cut(s) 759, 1438, 1664, 2145, 2174
BslFI GGGAC 4 cut(s) 406, 1265, 2329, 3283
BsmAI GTCTC 3 cut(s) 43, 391, 3316
BsmFI GGGAC 4 cut(s) 406, 1265, 2329, 3283
BsmI GAATGC 5 cut(s) 130, 226, 709, 2928, 3112
BsnI GGCC 5 cut(s) 568, 624, 647, 2937, 3183
Bso31I GGTCTC 1 cut(s) 3316
BsoBI CYCGRG 2 cut(s) 148, 2254
Bsp1286I GDGCHC 4 cut(s) 914, 2508, 2848, 3153
Bsp19I CCATGG 1 cut(s) 93
BspANI GGCC 5 cut(s) 568, 624, 647, 2937, 3183
BspCNI CTCAG 6 cut(s) 401, 622, 1630, 1900, 2004, 2020
BspFNI CGCG 2 cut(s) 183, 3176
BspHI TCATGA 1 cut(s) 1822
BspMAI CTGCAG 1 cut(s) 2125
BspMI ACCTGC 1 cut(s) 2114
BspOI GCTAGC 1 cut(s) 1379
BspPI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
BspT107I GGYRCC 3 cut(s) 234, 717, 2270
BspTNI GGTCTC 1 cut(s) 3316
BsrBI CCGCTC 1 cut(s) 2553
BsrDI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BsrFI RCCGGY 3 cut(s) 1663, 2144, 2173
BssAI RCCGGY 3 cut(s) 1663, 2144, 2173
BssECI CCNNGG 7 cut(s) 93, 349, 856, 1813, 2110, 2869, 3223
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 4 cut(s) 93, 1813, 2110, 2869
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 7 cut(s) 19, 351, 419, 858, 926, 1083, 3225
Bst4CI ACNGT 6 cut(s) 248, 1527, 2510, 2536, 3040, 3238
Bst6I CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
BstC8I GCNNGC 8 cut(s) 311, 781, 895, 1377, 1503, 1635, 2264, 3267
BstDEI CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 761
BstENI CCTNNNNNAGG 1 cut(s) 2121
BstFNI CGCG 2 cut(s) 183, 3176
BstHHI GCGC 2 cut(s) 1863, 3178
BstMAI GTCTC 3 cut(s) 43, 391, 3316
BstMCI CGRYCG 1 cut(s) 1840
BstNI CCWGG 7 cut(s) 19, 351, 419, 858, 926, 1083, 3225
BstNSI RCATGY 5 cut(s) 885, 1637, 2266, 2476, 2713
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 8 cut(s) 17, 349, 417, 856, 924, 1081, 1437, 3223
BstSFI CTRYAG 1 cut(s) 2121
BstSLI GKGCMC 2 cut(s) 2508, 2848
BstUI CGCG 2 cut(s) 183, 3176
BstV2I GAAGAC 1 cut(s) 2141
BstX2I RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
BstXI CCANNNNNNTGG 1 cut(s) 871
BstYI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
BsuRI GGCC 5 cut(s) 568, 624, 647, 2937, 3183
BtgI CCRYGG 1 cut(s) 93
BtrI CACGTC 2 cut(s) 2319, 2770
BveI ACCTGC 1 cut(s) 2114
Cac8I GCNNGC 8 cut(s) 311, 781, 895, 1377, 1503, 1635, 2264, 3267
CciI TCATGA 1 cut(s) 1822
CfoI GCGC 2 cut(s) 1863, 3178
Cfr10I RCCGGY 3 cut(s) 1663, 2144, 2173
Cfr13I GGNCC 9 cut(s) 566, 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
Csp6I GTAC 3 cut(s) 197, 235, 1755
CspAI ACCGGT 1 cut(s) 1663
CspCI CAANNNNNGTGG 2 cut(s) 2734, 2769
CviQI GTAC 3 cut(s) 197, 235, 1755
DdeI CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
DraI TTTAAA 1 cut(s) 1548
EaeI YGGCCR 2 cut(s) 645, 3181
Eam1104I CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
EarI CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
Ecl136II GAGCTC 1 cut(s) 3151
Eco130I CCWWGG 4 cut(s) 93, 1813, 2110, 2869
Eco24I GRGCYC 2 cut(s) 914, 3153
Eco31I GGTCTC 1 cut(s) 3316
Eco32I GATATC 3 cut(s) 838, 2698, 3121
Eco47I GGWCC 8 cut(s) 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
Eco53kI GAGCTC 1 cut(s) 3151
Eco57I CTGAAG 7 cut(s) 10, 383, 639, 890, 2053, 2135, 2213
Eco88I CYCGRG 2 cut(s) 148, 2254
Eco91I GGTNACC 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 3151
EcoNI CCTNNNNNAGG 1 cut(s) 2121
EcoO109I RGGNCCY 3 cut(s) 566, 1880, 2114
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 7 cut(s) 17, 349, 417, 856, 924, 1081, 3223
EcoRV GATATC 3 cut(s) 838, 2698, 3121
EcoT14I CCWWGG 4 cut(s) 93, 1813, 2110, 2869
EcoT38I GRGCYC 2 cut(s) 914, 3153
ErhI CCWWGG 4 cut(s) 93, 1813, 2110, 2869
FalI AAGNNNNNCTT 2 cut(s) 2997, 3029
FaqI GGGAC 4 cut(s) 406, 1265, 2329, 3283
FauI CCCGC 2 cut(s) 1524, 1588
FbaI TGATCA 1 cut(s) 2815
FblI GTMKAC 3 cut(s) 530, 1037, 2130
FriOI GRGCYC 2 cut(s) 914, 3153
FspBI CTAG 4 cut(s) 456, 1376, 1850, 2790
GlaI GCGC 2 cut(s) 1862, 3177
GsuI CTGGAG 6 cut(s) 237, 597, 720, 1104, 1108, 1977
HaeIII GGCC 5 cut(s) 568, 624, 647, 2937, 3183
HapII CCGG 5 cut(s) 759, 1438, 1664, 2145, 2174
HhaI GCGC 2 cut(s) 1863, 3178
Hin6I GCGC 2 cut(s) 1861, 3176
HinP1I GCGC 2 cut(s) 1861, 3176
HincII GTYRAC 8 cut(s) 531, 583, 1038, 1090, 2167, 2577, 2647, 3286
HindII GTYRAC 8 cut(s) 531, 583, 1038, 1090, 2167, 2577, 2647, 3286
HindIII AAGCTT 1 cut(s) 505
HpaII CCGG 5 cut(s) 759, 1438, 1664, 2145, 2174
HphI GGTGA 7 cut(s) 755, 1071, 1452, 1647, 1770, 1910, 2790
Hpy99I CGWCG 2 cut(s) 1042, 2774
HpyCH4III ACNGT 6 cut(s) 248, 1527, 2510, 2536, 3040, 3238
HpyCH4IV ACGT 4 cut(s) 1922, 2318, 2769, 3139
HpyF3I CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
HpySE526I ACGT 4 cut(s) 1922, 2318, 2769, 3139
HspAI GCGC 2 cut(s) 1861, 3176
KpnI GGTACC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 2815
LmnI GCTCC 9 cut(s) 322, 866, 890, 917, 1559, 1958, 2956, 3070, 3148
LweI GCATC 9 cut(s) 79, 1032, 1198, 1347, 2443, 2646, 2771, 2874, 3000
MaeI CTAG 4 cut(s) 456, 1376, 1850, 2790
MaeII ACGT 4 cut(s) 1922, 2318, 2769, 3139
MbiI CCGCTC 1 cut(s) 2553
MfeI CAATTG 2 cut(s) 2310, 2898
MflI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
MhlI GDGCHC 4 cut(s) 914, 2508, 2848, 3153
MlsI TGGCCA 1 cut(s) 647
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 8 cut(s) 45, 388, 393, 1250, 2246, 2491, 3091, 3305
MmeI TCCRAC 6 cut(s) 66, 868, 958, 2229, 2712, 3059
Mox20I TGGCCA 1 cut(s) 647
MroXI GAANNNNTTC 2 cut(s) 520, 985
MscI TGGCCA 1 cut(s) 647
MseI TTAA 5 cut(s) 1547, 1832, 2135, 2286, 2370
MslI CAYNNNNRTG 5 cut(s) 249, 2276, 2495, 2640, 3189
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 4 cut(s) 917, 1307, 1595, 2582
MspI CCGG 5 cut(s) 759, 1438, 1664, 2145, 2174
MspR9I CCNGG 8 cut(s) 19, 351, 419, 858, 926, 1083, 1439, 3225
MunI CAATTG 2 cut(s) 2310, 2898
Mva1269I GAATGC 5 cut(s) 130, 226, 709, 2928, 3112
MvaI CCWGG 7 cut(s) 19, 351, 419, 858, 926, 1083, 3225
MvnI CGCG 2 cut(s) 183, 3176
NciI CCSGG 1 cut(s) 1439
NcoI CCATGG 1 cut(s) 93
NheI GCTAGC 1 cut(s) 1375
NmeAIII GCCGAG 2 cut(s) 2247, 2913
NmuCI GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
NspI RCATGY 5 cut(s) 885, 1637, 2266, 2476, 2713
OliI CACNNNNGTG 1 cut(s) 2276
PaeI GCATGC 2 cut(s) 1637, 2266
PaeR7I CTCGAG 2 cut(s) 148, 2254
PagI TCATGA 1 cut(s) 1822
PaqCI CACCTGC 1 cut(s) 2114
PciI ACATGT 2 cut(s) 2472, 2709
PctI GAATGC 5 cut(s) 130, 226, 709, 2928, 3112
PdmI GAANNNNTTC 2 cut(s) 520, 985
PfeI GAWTC 6 cut(s) 167, 266, 295, 755, 2590, 3063
PflMI CCANNNNNTGG 2 cut(s) 643, 1082
PinAI ACCGGT 1 cut(s) 1663
PleI GAGTC 8 cut(s) 44, 388, 392, 1250, 2246, 2490, 3090, 3304
PpsI GAGTC 8 cut(s) 44, 388, 392, 1250, 2246, 2490, 3090, 3304
PpuMI RGGWCCY 2 cut(s) 1880, 2114
PscI ACATGT 2 cut(s) 2472, 2709
Psp124BI GAGCTC 1 cut(s) 3153
Psp1406I AACGTT 1 cut(s) 3139
Psp5II RGGWCCY 2 cut(s) 1880, 2114
Psp6I CCWGG 7 cut(s) 17, 349, 417, 856, 924, 1081, 3223
PspEI GGTNACC 1 cut(s) 761
PspGI CCWGG 7 cut(s) 17, 349, 417, 856, 924, 1081, 3223
PspPI GGNCC 9 cut(s) 566, 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
PspPPI RGGWCCY 2 cut(s) 1880, 2114
PspXI VCTCGAGB 1 cut(s) 2254
PstI CTGCAG 1 cut(s) 2125
PsuI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
PvuII CAGCTG 2 cut(s) 1307, 2582
RsaI GTAC 3 cut(s) 198, 236, 1756
RsaNI GTAC 3 cut(s) 197, 235, 1755
RseI CAYNNNNRTG 5 cut(s) 249, 2276, 2495, 2640, 3189
SacI GAGCTC 1 cut(s) 3153
SalI GTCGAC 2 cut(s) 529, 1036
SaqAI TTAA 5 cut(s) 1547, 1832, 2135, 2286, 2370
Sau96I GGNCC 9 cut(s) 566, 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
SchI GAGTC 8 cut(s) 45, 388, 393, 1250, 2246, 2491, 3091, 3305
ScrFI CCNGG 8 cut(s) 19, 351, 419, 858, 926, 1083, 1439, 3225
SduI GDGCHC 4 cut(s) 914, 2508, 2848, 3153
SfaNI GCATC 9 cut(s) 79, 1032, 1198, 1347, 2443, 2646, 2771, 2874, 3000
SfcI CTRYAG 1 cut(s) 2121
Sfr274I CTCGAG 2 cut(s) 148, 2254
SinI GGWCC 8 cut(s) 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
SlaI CTCGAG 2 cut(s) 148, 2254
SmiMI CAYNNNNRTG 5 cut(s) 249, 2276, 2495, 2640, 3189
SmlI CTYRAG 3 cut(s) 148, 2254, 3152
SmoI CTYRAG 3 cut(s) 148, 2254, 3152
SphI GCATGC 2 cut(s) 1637, 2266
SspMI CTAG 4 cut(s) 456, 1376, 1850, 2790
SstI GAGCTC 1 cut(s) 3153
StyD4I CCNGG 8 cut(s) 17, 349, 417, 856, 924, 1081, 1437, 3223
StyI CCWWGG 4 cut(s) 93, 1813, 2110, 2869
TaaI ACNGT 6 cut(s) 248, 1527, 2510, 2536, 3040, 3238
TaiI ACGT 4 cut(s) 1925, 2321, 2772, 3142
TaqI TCGA 9 cut(s) 149, 442, 530, 1037, 1259, 2255, 2529, 2593, 2772
TatI WGTACW 1 cut(s) 1754
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 6 cut(s) 167, 266, 295, 755, 2590, 3063
Tru1I TTAA 5 cut(s) 1547, 1832, 2135, 2286, 2370
Tru9I TTAA 5 cut(s) 1547, 1832, 2135, 2286, 2370
TseFI GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
Tsp45I GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
TspDTI ATGAA 6 cut(s) 578, 1149, 1839, 2067, 2344, 2415
TspGWI ACGGA 2 cut(s) 859, 2969
Van91I CCANNNNNTGG 2 cut(s) 643, 1082
VneI GTGCAC 1 cut(s) 2844
VpaK11BI GGWCC 8 cut(s) 1073, 1453, 1586, 1817, 1880, 1967, 2114, 2894
XagI CCTNNNNNAGG 1 cut(s) 2121
XapI RAATTY 8 cut(s) 342, 518, 640, 849, 1012, 1159, 2237, 3091
XceI RCATGY 5 cut(s) 885, 1637, 2266, 2476, 2713
XcmI CCANNNNNNNNNTGG 4 cut(s) 91, 212, 695, 2012
XhoI CTCGAG 2 cut(s) 148, 2254
XmiI GTMKAC 3 cut(s) 530, 1037, 2130
XmnI GAANNNNTTC 2 cut(s) 520, 985
XspI CTAG 4 cut(s) 456, 1376, 1850, 2790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.