Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Reverse (-)

4705648 .. 4709735

Gene Structure 4088 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGTGACCATGGCCGAACTGGAAGAGCAAGTTCCCGCCATGAGAGCTCTAGGCTCTCTCTTCAAGCTCACCGAAGTCTTTCTCTGGTTCAAACCAGCTGGCGACGGCGGCGATGAATGTGGGACTTTGGCGGAGGATATGGAGCTTACTAACCAGATGAATGCGCTGGGGCTTCCAGTCTCATTCAACACTAATAAGGAGAAGAGGAACAGAAAGACGGAGAGTAGAAGAAAGGGAATGCGCCTGAAGCAACCAGACAGTTCTCTGGACGTTGTAGGTGGAGCAATGGAGCCTTCCAAAGTGAGTGAGGGGGAGATTGTTTCTCCTATGATATTCGATGGTAACACAAGCAGTTCTTTGTGTTGTTTGCCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCGGGTGCTGTTGAATTTCAGTGCCCTTGTGTTGAAGGAGATAATCCAGAAAATTCAACTGAGATTACTGGTGATGCTGTCAAAGAACAAGATCGTGATGGAATATTGGCCGTCGTTTGTAATGATGCCCAGGGTTGTGACCCTTTACACAGGGGCCATGTGCTCAATGACATAATGAGAATTGCGGTGAGCTCAACTGATTTAGATGCTGGACGTTGCCCTGAAAGCTGCTCAGCAGATGCTGCTGTTGGCAATGATGAGACAGAATCAGGTGAAATATTAATGGAGCATGATCACTTAGAGTGTTCATTAGTGGCTTCCCACGAAGCAGAACTTACCAAACCATGCGAGGACTATATCCCTGAACCGCAACAGGTTTCTGAGTCAATTTCATATTCCATGTGTTCAGAAGTGCTTGACCCTGATGGAACTGACTGCCAAGACAACGGTGATTGCGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATCAAAACGCATACTTCCACATGGTATCCACCCCCAGGCATGGAATATTTAGCATCTATTGATGCCATATGTAAGCCAAATGATGTGATTTCTGAAGTAATGGAGATTGATGTTAGCACTGACTCAAAGGCAACAGATTTATGTGGTGTGAGTAAAACTGATTCATTCCAAGAATCAATTACTCAAGATGTTTCACAGGGTCAGCCATATCATGAGATCTCCGGGGGGATTGAACTCACTGTTGACACTTCTATGTCTGAAACCACTTTGTCAACTGTCACTGTAAGCAGATGTCCGGTACATTCAGATGAAATCGACGAGAACAATAACACCTGCAATGATGGGAATGCATCATGCTTCTCATCAGATGTCCAGGATCATATCGCTAGTATCAGAAACAAAACCAAGCAGTTGATTTCTGATGATGTCTGCAACAGTGGTTTGCAACCAATTCTTGCTGAACAGATTGATGAACAAAATACTATTGAACTCAATAATGAGCGTAATGAACCTAATTTTTGTCAGGAAACTCTCAAAGATTGTGAAGATTTTGATGCGTTTCAAATATTAAATACAAGCAGCTTGTCCCATAAATACACTGATGAAGTGTCTGAGGATAGTAATATGCATTCAGGAAATGAAGTTTTGGCAACAAACCAGTTGGAAATGCAGCTTGACCCTGCTGTACAAAAACGGAAGAAGAAAGTGAGAAGAAAGAAAATTCAGAGAAAATTATCTAATGAGAACGAAGAGCTTCTATTTCAAGAGCTGTTCAAAGAGTTTTCTGCTGATATGGGAAAATATTGGTGTCAACGGTACCTATTATTCTCCAGATACGATGATGGTATAAAAATGGATGAGGAAGGATGGTTCTCTGTCACTCCAGAGCTTCTAGCTAGGCATCATGCAGAAAGATGTGGTAGTGACGTCATCATTGACTGTTTTACTGGAGTTGGTGGGAATTCCATCCAATTTGCACAGATAAGCAAACATGTAATTGCAATTGATATTGATCGATCGAAGATTGATTATGCACAGCATAATGCTGCCATCTATGGGGTTGATGACAGGATAGATTTCATAAAGGGGGACTTTTTCCGCCTGGCACCAAAGCTGAAGGCAGACACGGTCTTTTTGTCACCCCCGTGGGGAGGACCTGATTATGCAAAAGTAGAGACATACGACATGAAGACAATGCTTAAGCCACATGATGGATATTTTCTCTTTAACATTGCAAAGGAAGTTGCTTCTAGGATTGTCATGTTTCTCCCAAGAAATGTTGATATCAACCAATTAGCAGAGATCGCTCTATCGGGGTCTCAGCCATGGTCACTAGAGGTCGAGAAAAACTTCTTAAATGGCAAGTTGAAGGGGATAACTGCTTACTTCAGTGACATGGCCAGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

777

Amino Acids

86.35

Weight (kDa)

4.49

Isoelectric Point (pI)

40.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TRM5-TYW2_MTfase	PF02475	615 - 687	1.5e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_15	PF09445	617 - 771	5.9e-44	RNA cap guanine-N2 methyltransferase
Methyltransf_31	PF13847	617 - 698	3.3e-07	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1262
AatII	GACGTC	1 cut(s)	1851
Acc36I	ACCTGC	1 cut(s)	1262
Acc65I	GGTACC	1 cut(s)	1737
AccB1I	GGYRCC	2 cut(s)	1737, 2026
AccB7I	CCANNNNNTGG	1 cut(s)	2132
AciI	CCGC	8 cut(s)	36, 108, 131, 413, 596, 779, 867, 2020
AclWI	GGATC	1 cut(s)	1305
AcoI	YGGCCR	3 cut(s)	12, 519, 2319
AcsI	RAATTY	4 cut(s)	425, 463, 1641, 1882
AcuI	CTGAAG	4 cut(s)	266, 1035, 2057, 2293
AcyI	GRCGYC	1 cut(s)	1848
AfaI	GTAC	3 cut(s)	1221, 1608, 1739
AfiI	CCNNNNNNNGG	7 cut(s)	377, 956, 961, 1977, 2069, 2072, 2132
AflII	CTTAAG	1 cut(s)	2120
AflIII	ACRYGT	1 cut(s)	1912
AjnI	CCWGG	4 cut(s)	540, 955, 1293, 2022
AleI	CACNNNNGTG	1 cut(s)	2065
AloI	GAACNNNNNNTCC	2 cut(s)	1775, 1807
Alw21I	GWGCWC	3 cut(s)	49, 576, 605
Alw26I	GTCTC	5 cut(s)	184, 665, 864, 2090, 2244
AlwI	GGATC	1 cut(s)	1305
AlwNI	CAGNNNCTG	1 cut(s)	653
AoxI	GGCC	5 cut(s)	12, 379, 519, 565, 2319
ApeKI	GCWGC	6 cut(s)	410, 639, 653, 1500, 1591, 1967
ApoI	RAATTY	4 cut(s)	425, 463, 1641, 1882
AseI	ATTAAT	1 cut(s)	692
Asp700I	GAANNNNTTC	3 cut(s)	78, 1674, 2270
Asp718I	GGTACC	1 cut(s)	1737
AspLEI	GCGC	2 cut(s)	166, 243
AspS9I	GGNCC	3 cut(s)	379, 565, 2075
AsuC2I	CCSGG	1 cut(s)	1144
AsuHPI	GGTGA	7 cut(s)	16, 61, 494, 610, 695, 872, 2052
AvaII	GGWCC	1 cut(s)	2075
BaeGI	GKGCMC	1 cut(s)	437
BalI	TGGCCA	1 cut(s)	2321
BanI	GGYRCC	2 cut(s)	1737, 2026
BanII	GRGCYC	2 cut(s)	49, 605
BbsI	GAAGAC	1 cut(s)	2117
Bbv12I	GWGCWC	3 cut(s)	49, 576, 605
BbvI	GCAGC	6 cut(s)	422, 626, 640, 1512, 1603, 1954
BceAI	ACGGC	2 cut(s)	121, 506
BciT130I	CCWGG	4 cut(s)	542, 957, 1295, 2024
BciVI	GTATCC	1 cut(s)	957
BclI	TGATCA	1 cut(s)	703
BcnI	CCSGG	1 cut(s)	1144
BcoDI	GTCTC	5 cut(s)	184, 665, 864, 2090, 2244
BfaI	CTAG	6 cut(s)	50, 1308, 1814, 1818, 2172, 2255
BfrI	CTTAAG	1 cut(s)	2120
BfuAI	ACCTGC	1 cut(s)	1262
BfuI	GTATCC	1 cut(s)	957
BglII	AGATCT	1 cut(s)	1137
BisI	GCNGC	7 cut(s)	109, 411, 640, 654, 1501, 1592, 1968
BlpI	GCTNAGC	1 cut(s)	643
BlsI	GCNGC	7 cut(s)	110, 412, 641, 655, 1502, 1593, 1969
Bme1390I	CCNGG	5 cut(s)	542, 957, 1144, 1295, 2024
Bme18I	GGWCC	1 cut(s)	2075
BmgT120I	GGNCC	3 cut(s)	379, 565, 2075
BmiI	GGNNCC	4 cut(s)	291, 566, 1739, 2028
BmrFI	CCNGG	5 cut(s)	542, 957, 1144, 1295, 2024
BmsI	GCATC	9 cut(s)	475, 526, 607, 640, 973, 983, 1280, 1465, 1831
BpiI	GAAGAC	1 cut(s)	2117
BpmI	CTGGAG	3 cut(s)	1735, 1788, 1890
Bpu1102I	GCTNAGC	1 cut(s)	643
BpuEI	CTTGAG	1 cut(s)	1089
BpuMI	CCSGG	1 cut(s)	1144
Bsa29I	ATCGAT	1 cut(s)	1936
BsaBI	GATNNNNATC	1 cut(s)	1932
BsaHI	GRCGYC	1 cut(s)	1848
BsaI	GGTCTC	1 cut(s)	2244
BsaJI	CCNNGG	7 cut(s)	9, 540, 541, 955, 1143, 2066, 2246
BsaWI	WCCGGW	1 cut(s)	1216
Bsc4I	CCNNNNNNNGG	7 cut(s)	377, 956, 961, 1977, 2069, 2072, 2132
Bse1I	ACTGG	7 cut(s)	24, 176, 484, 878, 1579, 1873, 2322
Bse3DI	GCAATG	4 cut(s)	291, 670, 1264, 2151
Bse8I	GATNNNNATC	1 cut(s)	1932
BseBI	CCWGG	4 cut(s)	542, 957, 1295, 2024
BseCI	ATCGAT	1 cut(s)	1936
BseDI	CCNNGG	7 cut(s)	9, 540, 541, 955, 1143, 2066, 2246
BseGI	GGATG	4 cut(s)	882, 1783, 1793, 1887
BseJI	GATNNNNATC	1 cut(s)	1932
BseLI	CCNNNNNNNGG	7 cut(s)	377, 956, 961, 1977, 2069, 2072, 2132
BseMI	GCAATG	4 cut(s)	291, 670, 1264, 2151
BseMII	CTCAG	5 cut(s)	462, 657, 783, 1524, 2255
BseNI	ACTGG	7 cut(s)	24, 176, 484, 878, 1579, 1873, 2322
BseSI	GKGCMC	1 cut(s)	437
BseXI	GCAGC	6 cut(s)	422, 626, 640, 1512, 1603, 1954
BseYI	CCCAGC	1 cut(s)	166
Bsh1285I	CGRYCG	1 cut(s)	1940
BshFI	GGCC	5 cut(s)	14, 381, 521, 567, 2321
BshNI	GGYRCC	2 cut(s)	1737, 2026
BshVI	ATCGAT	1 cut(s)	1936
BsiEI	CGRYCG	1 cut(s)	1940
BsiHKAI	GWGCWC	3 cut(s)	49, 576, 605
BsiSI	CCGG	2 cut(s)	1143, 1217
BslFI	GGGAC	3 cut(s)	136, 1492, 2024
BslI	CCNNNNNNNGG	7 cut(s)	377, 956, 961, 1977, 2069, 2072, 2132
BsmAI	GTCTC	5 cut(s)	184, 665, 864, 2090, 2244
BsmFI	GGGAC	3 cut(s)	136, 1492, 2024
BsmI	GAATGC	4 cut(s)	166, 243, 1273, 1549
BsnI	GGCC	5 cut(s)	14, 381, 521, 567, 2321
Bso31I	GGTCTC	1 cut(s)	2244
Bsp1286I	GDGCHC	4 cut(s)	49, 437, 576, 605
Bsp1407I	TGTACA	1 cut(s)	1606
Bsp143I	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
Bsp1720I	GCTNAGC	1 cut(s)	643
Bsp19I	CCATGG	2 cut(s)	9, 2246
BspACI	CCGC	8 cut(s)	36, 108, 131, 413, 596, 779, 867, 2020
BspANI	GGCC	5 cut(s)	14, 381, 521, 567, 2321
BspCNI	CTCAG	5 cut(s)	463, 656, 784, 1525, 2254
BspDI	ATCGAT	1 cut(s)	1936
BspHI	TCATGA	1 cut(s)	1132
BspLI	GGNNCC	4 cut(s)	291, 566, 1739, 2028
BspMI	ACCTGC	1 cut(s)	1262
BspPI	GGATC	1 cut(s)	1305
BspQI	GCTCTTC	2 cut(s)	18, 1665
BspT107I	GGYRCC	2 cut(s)	1737, 2026
BspTI	CTTAAG	1 cut(s)	2120
BspTNI	GGTCTC	1 cut(s)	2244
BsrDI	GCAATG	4 cut(s)	291, 670, 1264, 2151
BsrGI	TGTACA	1 cut(s)	1606
BsrI	ACTGG	7 cut(s)	24, 176, 484, 878, 1579, 1873, 2322
BssECI	CCNNGG	7 cut(s)	9, 540, 541, 955, 1143, 2066, 2246
BssMI	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
BssNI	GRCGYC	1 cut(s)	1848
BssT1I	CCWWGG	2 cut(s)	9, 2246
Bst2UI	CCWGG	4 cut(s)	542, 957, 1295, 2024
Bst4CI	ACNGT	9 cut(s)	260, 860, 1162, 1198, 1204, 1358, 1737, 1862, 2050
Bst6I	CTCTTC	4 cut(s)	18, 65, 197, 1665
BstACI	GRCGYC	1 cut(s)	1848
BstAFI	CTTAAG	1 cut(s)	2120
BstAPI	GCANNNNNTGC	2 cut(s)	416, 653
BstAUI	TGTACA	1 cut(s)	1606
BstC8I	GCNNGC	1 cut(s)	100
BstDEI	CTNAG	6 cut(s)	471, 643, 709, 792, 1533, 2241
BstDSI	CCRYGG	3 cut(s)	9, 2066, 2246
BstEII	GGTNACC	1 cut(s)	4
BstF5I	GGATG	4 cut(s)	882, 1783, 1793, 1887
BstHHI	GCGC	2 cut(s)	166, 243
BstKTI	GATC	7 cut(s)	505, 706, 1140, 1300, 1936, 1940, 2226
BstMAI	GTCTC	5 cut(s)	184, 665, 864, 2090, 2244
BstMBI	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
BstMCI	CGRYCG	1 cut(s)	1940
BstMWI	GCNNNNNNNGC	7 cut(s)	44, 108, 247, 416, 636, 653, 2225
BstNI	CCWGG	4 cut(s)	542, 957, 1295, 2024
BstNSI	RCATGY	1 cut(s)	1916
BstPI	GGTNACC	1 cut(s)	4
BstSCI	CCNGG	5 cut(s)	540, 955, 1142, 1293, 2022
BstSLI	GKGCMC	1 cut(s)	437
BstV1I	GCAGC	6 cut(s)	422, 626, 640, 1512, 1603, 1954
BstV2I	GAAGAC	1 cut(s)	2117
BstX2I	RGATCY	1 cut(s)	1137
BstYI	RGATCY	1 cut(s)	1137
Bsu15I	ATCGAT	1 cut(s)	1936
BsuI	GTATCC	1 cut(s)	957
BsuRI	GGCC	5 cut(s)	14, 381, 521, 567, 2321
BsuTUI	ATCGAT	1 cut(s)	1936
BtgI	CCRYGG	3 cut(s)	9, 2066, 2246
BtgZI	GCGATG	1 cut(s)	126
BtsCI	GGATG	4 cut(s)	882, 1783, 1793, 1887
BtsIMutI	CAGTG	7 cut(s)	437, 1038, 1158, 1200, 1363, 1518, 2317
BveI	ACCTGC	1 cut(s)	1262
Cac8I	GCNNGC	1 cut(s)	100
CaiI	CAGNNNCTG	1 cut(s)	653
CciI	TCATGA	1 cut(s)	1132
CfoI	GCGC	2 cut(s)	166, 243
Cfr13I	GGNCC	3 cut(s)	379, 565, 2075
ClaI	ATCGAT	1 cut(s)	1936
Csp6I	GTAC	3 cut(s)	1220, 1607, 1738
CviQI	GTAC	3 cut(s)	1220, 1607, 1738
DdeI	CTNAG	6 cut(s)	471, 643, 709, 792, 1533, 2241
DpnI	GATC	7 cut(s)	504, 705, 1139, 1299, 1935, 1939, 2225
DpnII	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
EaeI	YGGCCR	3 cut(s)	12, 519, 2319
Eam1104I	CTCTTC	4 cut(s)	18, 65, 197, 1665
EarI	CTCTTC	4 cut(s)	18, 65, 197, 1665
EciI	GGCGGA	2 cut(s)	146, 2009
Ecl136II	GAGCTC	2 cut(s)	47, 603
Eco130I	CCWWGG	2 cut(s)	9, 2246
Eco24I	GRGCYC	2 cut(s)	49, 605
Eco31I	GGTCTC	1 cut(s)	2244
Eco32I	GATATC	1 cut(s)	2206
Eco47I	GGWCC	1 cut(s)	2075
Eco53kI	GAGCTC	2 cut(s)	47, 603
Eco57I	CTGAAG	4 cut(s)	266, 1035, 2057, 2293
Eco91I	GGTNACC	1 cut(s)	4
EcoICRI	GAGCTC	2 cut(s)	47, 603
EcoO109I	RGGNCCY	1 cut(s)	2075
EcoO65I	GGTNACC	1 cut(s)	4
EcoRI	GAATTC	1 cut(s)	1882
EcoRII	CCWGG	4 cut(s)	540, 955, 1293, 2022
EcoRV	GATATC	1 cut(s)	2206
EcoT14I	CCWWGG	2 cut(s)	9, 2246
EcoT22I	ATGCAT	2 cut(s)	1273, 1551
EcoT38I	GRGCYC	2 cut(s)	49, 605
ErhI	CCWWGG	2 cut(s)	9, 2246
FalI	AAGNNNNNCTT	6 cut(s)	340, 372, 729, 761, 1997, 2029
FaqI	GGGAC	3 cut(s)	136, 1492, 2024
FauI	CCCGC	2 cut(s)	43, 406
FauNDI	CATATG	1 cut(s)	989
FbaI	TGATCA	1 cut(s)	703
Fnu4HI	GCNGC	7 cut(s)	109, 411, 640, 654, 1501, 1592, 1968
FokI	GGATG	4 cut(s)	889, 1790, 1800, 1874
FriOI	GRGCYC	2 cut(s)	49, 605
Fsp4HI	GCNGC	7 cut(s)	109, 411, 640, 654, 1501, 1592, 1968
FspBI	CTAG	6 cut(s)	50, 1308, 1814, 1818, 2172, 2255
GlaI	GCGC	2 cut(s)	165, 242
GluI	GCNGC	7 cut(s)	109, 411, 640, 654, 1501, 1592, 1968
GsaI	CCCAGC	1 cut(s)	170
GsuI	CTGGAG	3 cut(s)	1735, 1788, 1890
HaeIII	GGCC	5 cut(s)	14, 381, 521, 567, 2321
HapII	CCGG	2 cut(s)	1143, 1217
HhaI	GCGC	2 cut(s)	166, 243
Hin1I	GRCGYC	1 cut(s)	1848
Hin6I	GCGC	2 cut(s)	164, 241
HinP1I	GCGC	2 cut(s)	164, 241
HincII	GTYRAC	3 cut(s)	1165, 1194, 1733
HindII	GTYRAC	3 cut(s)	1165, 1194, 1733
HinfI	GANTC	7 cut(s)	389, 677, 794, 890, 1043, 1082, 1094
HpaII	CCGG	2 cut(s)	1143, 1217
HphI	GGTGA	7 cut(s)	16, 61, 494, 610, 695, 872, 2052
Hpy166II	GTNNAC	3 cut(s)	1165, 1194, 1733
Hpy8I	GTNNAC	3 cut(s)	1165, 1194, 1733
Hpy99I	CGWCG	3 cut(s)	107, 527, 1241
HpyAV	CCTTC	5 cut(s)	303, 440, 1778, 2032, 2284
HpyCH4III	ACNGT	9 cut(s)	260, 860, 1162, 1198, 1204, 1358, 1737, 1862, 2050
HpyCH4IV	ACGT	3 cut(s)	270, 625, 1848
HpyF10VI	GCNNNNNNNGC	7 cut(s)	44, 108, 247, 416, 636, 653, 2225
HpyF3I	CTNAG	6 cut(s)	471, 643, 709, 792, 1533, 2241
HpySE526I	ACGT	3 cut(s)	270, 625, 1848
Hsp92I	GRCGYC	1 cut(s)	1848
HspAI	GCGC	2 cut(s)	164, 241
KpnI	GGTACC	1 cut(s)	1741
Ksp22I	TGATCA	1 cut(s)	703
Kzo9I	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
LguI	GCTCTTC	2 cut(s)	18, 1665
LmnI	GCTCC	4 cut(s)	142, 281, 289, 697
Lsp1109I	GCAGC	6 cut(s)	422, 626, 640, 1512, 1603, 1954
LweI	GCATC	9 cut(s)	475, 526, 607, 640, 973, 983, 1280, 1465, 1831
MaeI	CTAG	6 cut(s)	50, 1308, 1814, 1818, 2172, 2255
MaeII	ACGT	3 cut(s)	270, 625, 1848
MaeIII	GTNAC	9 cut(s)	4, 341, 548, 1198, 1798, 1844, 2058, 2250, 2312
MalI	GATC	7 cut(s)	504, 705, 1139, 1299, 1935, 1939, 2225
MboI	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
MfeI	CAATTG	1 cut(s)	1923
MflI	RGATCY	1 cut(s)	1137
MhlI	GDGCHC	4 cut(s)	49, 437, 576, 605
MlsI	TGGCCA	1 cut(s)	2321
MluNI	TGGCCA	1 cut(s)	2321
MlyI	GAGTC	2 cut(s)	803, 1037
MmeI	TCCRAC	1 cut(s)	1563
MnlI	CCTC	9 cut(s)	127, 198, 301, 409, 754, 1528, 1774, 2066, 2251
Mox20I	TGGCCA	1 cut(s)	2321
Mph1103I	ATGCAT	2 cut(s)	1273, 1551
MroXI	GAANNNNTTC	3 cut(s)	78, 1674, 2270
MscI	TGGCCA	1 cut(s)	2321
MseI	TTAA	5 cut(s)	692, 1490, 2121, 2148, 2276
MslI	CAYNNNNRTG	3 cut(s)	1172, 1227, 2065
Msp20I	TGGCCA	1 cut(s)	2321
MspA1I	CMGCKG	2 cut(s)	98, 413
MspCI	CTTAAG	1 cut(s)	2120
MspI	CCGG	2 cut(s)	1143, 1217
MspR9I	CCNGG	5 cut(s)	542, 957, 1144, 1295, 2024
MunI	CAATTG	1 cut(s)	1923
Mva1269I	GAATGC	4 cut(s)	166, 243, 1273, 1549
MvaI	CCWGG	4 cut(s)	542, 957, 1295, 2024
MwoI	GCNNNNNNNGC	7 cut(s)	44, 108, 247, 416, 636, 653, 2225
NciI	CCSGG	1 cut(s)	1144
NcoI	CCATGG	2 cut(s)	9, 2246
NdeI	CATATG	1 cut(s)	989
NdeII	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
NlaIV	GGNNCC	4 cut(s)	291, 566, 1739, 2028
NmuCI	GTSAC	8 cut(s)	4, 548, 1198, 1798, 1844, 2058, 2250, 2312
NsiI	ATGCAT	2 cut(s)	1273, 1551
NspI	RCATGY	1 cut(s)	1916
OliI	CACNNNNGTG	1 cut(s)	2065
PagI	TCATGA	1 cut(s)	1132
PaqCI	CACCTGC	1 cut(s)	1262
PasI	CCCWGGG	1 cut(s)	541
PciI	ACATGT	1 cut(s)	1912
PciSI	GCTCTTC	2 cut(s)	18, 1665
PctI	GAATGC	4 cut(s)	166, 243, 1273, 1549
PdmI	GAANNNNTTC	3 cut(s)	78, 1674, 2270
PfeI	GAWTC	5 cut(s)	389, 677, 890, 1082, 1094
PflFI	GACNNNGTC	1 cut(s)	2048
PflMI	CCANNNNNTGG	1 cut(s)	2132
PfoI	TCCNGGA	1 cut(s)	1293
PkrI	GCNGC	7 cut(s)	110, 412, 641, 655, 1502, 1593, 1969
Ple19I	CGATCG	1 cut(s)	1940
PleI	GAGTC	2 cut(s)	802, 1037
PpsI	GAGTC	2 cut(s)	802, 1037
PpuMI	RGGWCCY	1 cut(s)	2075
PscI	ACATGT	1 cut(s)	1912
PshBI	ATTAAT	1 cut(s)	692
Psp124BI	GAGCTC	2 cut(s)	49, 605
Psp5II	RGGWCCY	1 cut(s)	2075
Psp6I	CCWGG	4 cut(s)	540, 955, 1293, 2022
PspEI	GGTNACC	1 cut(s)	4
PspFI	CCCAGC	1 cut(s)	166
PspGI	CCWGG	4 cut(s)	540, 955, 1293, 2022
PspN4I	GGNNCC	4 cut(s)	291, 566, 1739, 2028
PspPI	GGNCC	3 cut(s)	379, 565, 2075
PspPPI	RGGWCCY	1 cut(s)	2075
PstNI	CAGNNNCTG	1 cut(s)	653
PsuI	RGATCY	1 cut(s)	1137
PsyI	GACNNNGTC	1 cut(s)	2048
PvuI	CGATCG	1 cut(s)	1940
PvuII	CAGCTG	1 cut(s)	98
RsaI	GTAC	3 cut(s)	1221, 1608, 1739
RsaNI	GTAC	3 cut(s)	1220, 1607, 1738
RseI	CAYNNNNRTG	3 cut(s)	1172, 1227, 2065
SacI	GAGCTC	2 cut(s)	49, 605
SapI	GCTCTTC	2 cut(s)	18, 1665
SaqAI	TTAA	5 cut(s)	692, 1490, 2121, 2148, 2276
SatI	GCNGC	7 cut(s)	109, 411, 640, 654, 1501, 1592, 1968
Sau3AI	GATC	7 cut(s)	502, 703, 1137, 1297, 1933, 1937, 2223
Sau96I	GGNCC	3 cut(s)	379, 565, 2075
SchI	GAGTC	2 cut(s)	803, 1037
ScrFI	CCNGG	5 cut(s)	542, 957, 1144, 1295, 2024
SduI	GDGCHC	4 cut(s)	49, 437, 576, 605
SfaNI	GCATC	9 cut(s)	475, 526, 607, 640, 973, 983, 1280, 1465, 1831
SinI	GGWCC	1 cut(s)	2075
SmiMI	CAYNNNNRTG	3 cut(s)	1172, 1227, 2065
SmlI	CTYRAG	2 cut(s)	1104, 2120
SmoI	CTYRAG	2 cut(s)	1104, 2120
SsiI	CCGC	8 cut(s)	36, 108, 131, 413, 596, 779, 867, 2020
SspI	AATATT	5 cut(s)	516, 690, 968, 1488, 1724
SspMI	CTAG	6 cut(s)	50, 1308, 1814, 1818, 2172, 2255
SstI	GAGCTC	2 cut(s)	49, 605
StyD4I	CCNGG	5 cut(s)	540, 955, 1142, 1293, 2022
StyI	CCWWGG	2 cut(s)	9, 2246
TaaI	ACNGT	9 cut(s)	260, 860, 1162, 1198, 1204, 1358, 1737, 1862, 2050
TaiI	ACGT	3 cut(s)	273, 628, 1851
TaqI	TCGA	5 cut(s)	336, 1236, 1936, 1940, 2262
TatI	WGTACW	1 cut(s)	1606
TauI	GCSGC	1 cut(s)	111
TfiI	GAWTC	5 cut(s)	389, 677, 890, 1082, 1094
Tru1I	TTAA	5 cut(s)	692, 1490, 2121, 2148, 2276
Tru9I	TTAA	5 cut(s)	692, 1490, 2121, 2148, 2276
TscAI	CASTG	7 cut(s)	437, 1045, 1165, 1207, 1363, 1525, 2317
TseFI	GTSAC	8 cut(s)	4, 548, 1198, 1798, 1844, 2058, 2250, 2312
TseI	GCWGC	6 cut(s)	410, 639, 653, 1500, 1591, 1967
Tsp45I	GTSAC	8 cut(s)	4, 548, 1198, 1798, 1844, 2058, 2250, 2312
TspGWI	ACGGA	2 cut(s)	233, 1630
TspRI	CASTG	7 cut(s)	437, 1045, 1165, 1207, 1363, 1525, 2317
Tth111I	GACNNNGTC	1 cut(s)	2048
Van91I	CCANNNNNTGG	1 cut(s)	2132
Vha464I	CTTAAG	1 cut(s)	2120
VpaK11BI	GGWCC	1 cut(s)	2075
VspI	ATTAAT	1 cut(s)	692
XapI	RAATTY	4 cut(s)	425, 463, 1641, 1882
XceI	RCATGY	1 cut(s)	1916
XcmI	CCANNNNNNNNNTGG	1 cut(s)	16
XmnI	GAANNNNTTC	3 cut(s)	78, 1674, 2270
XspI	CTAG	6 cut(s)	50, 1308, 1814, 1818, 2172, 2255
ZraI	GACGTC	1 cut(s)	1849
Zsp2I	ATGCAT	2 cut(s)	1273, 1551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.