pycom15g07630

RNA cap guanine-N2 methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014673
Physical Location & Seq
Reverse (-)
4714979 .. 4716359
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGTCTGATCCCACTTTGTCAACTGTCACTGTAAGCAGATGTCCGGTACAGTCGGATGAAATCAATGAGAACAATAACACCTGCAACGATGGGAACCTATCACGCTTCTTATCAGATGTCCAGGATCATATCGCTAGTATCAGAAACAAAATCAAGCAGTTTTCTTTGCATGGTGCCCACAGTATCAGAAACAAAATCAAGCAGTTGATTTCTTATGATGTCTGCAACAGTGGTTTGCAATTAATTCTTGCTGAACAGATTGATGAACAAAATACTATTGAACTCAATAATGAGCCTAATGAACCTAATTTTTGTGAGGAAACTCTCAAAGATTGCGAAGCTTTTGACACGTTTCAAATACTAAATACAAGCAGCTTGTCCTATACATACACTGACGAAGTATCTGAGGATAGTAATATGCATTCAGGAAATGAAGTTTTGGCAACAAACCAGTTGGAAATGCAGCTTGACCCTGCTGTACAAAAACGGAAGAAGAAAGTGAGAAGAAAGAAAATTCAGAGAAAGTTATCTAATGAAAACGGAGAGTTGTTCATAGAGTTTTCTGCTGATATGGAAAAATATTGGTATCAACGGTACCTATTATTCTCCAGATACGATGATGGTATAAAAATGGATGAGGAAGGATGGTTCTCTGTCACTCCAGAGCTTCTAGCTAGGCATCATGCAGAACGATGTGGTAGTGATGTCATCATTGATTGTTTTACTGGAGTTGGTGGGAATTCCATCCAATTTGCACAGATAAGCAAACATGTAATTGCAATTGACATTGATCGATCCTTATACGAAGATTGA

Protein Analysis

271

Amino Acids

30.95

Weight (kDa)

4.86

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_15 PF09445 236 - 266 3e-06 RNA cap guanine-N2 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34310 FvH4_2g34310
malus_domestica MD15G1081500.v1.1
prunus_persica Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis pycom15g07620 pycom15g07630
rosa_chinensis RchiOBHm_Chr6g0308051
rosa_laevigata RLG00000010672
rosa_multiflora Rmu_sc0002624.1_g000003
rosa_roxburghii Rroxscaffold_7G00160630
rosa_rugosa Rorug06G0363400
rosa_samantha Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana Rw6G041220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 89
Acc65I GGTACC 1 cut(s) 594
AccB1I GGYRCC 2 cut(s) 173, 594
AclWI GGATC 3 cut(s) 2, 132, 789
AcsI RAATTY 2 cut(s) 513, 739
AfaI GTAC 3 cut(s) 48, 480, 596
AflIII ACRYGT 2 cut(s) 348, 769
AgsI TTSAA 2 cut(s) 281, 356
AjnI CCWGG 1 cut(s) 120
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 5 cut(s) 341, 375, 466, 667, 674
AluI AGCT 5 cut(s) 341, 375, 466, 667, 674
AlwI GGATC 3 cut(s) 2, 132, 789
ApeKI GCWGC 2 cut(s) 372, 463
ApoI RAATTY 2 cut(s) 513, 739
AseI ATTAAT 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 594
BaeGI GKGCMC 1 cut(s) 178
BanI GGYRCC 2 cut(s) 173, 594
BbvI GCAGC 2 cut(s) 384, 475
BccI CCATC 4 cut(s) 83, 614, 639, 752
BciT130I CCWGG 1 cut(s) 122
BfaI CTAG 3 cut(s) 135, 671, 675
BfuAI ACCTGC 1 cut(s) 89
BisI GCNGC 2 cut(s) 373, 464
BlsI GCNGC 2 cut(s) 374, 465
Bme1390I CCNGG 1 cut(s) 122
BmiI GGNNCC 3 cut(s) 95, 175, 596
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 688
BpmI CTGGAG 3 cut(s) 592, 645, 747
Bsa29I ATCGAT 1 cut(s) 793
BsaWI WCCGGW 1 cut(s) 43
Bse1I ACTGG 2 cut(s) 451, 730
BseBI CCWGG 1 cut(s) 122
BseCI ATCGAT 1 cut(s) 793
BseGI GGATG 4 cut(s) 61, 640, 650, 744
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 451, 730
BseSI GKGCMC 1 cut(s) 178
BseXI GCAGC 2 cut(s) 384, 475
BshNI GGYRCC 2 cut(s) 173, 594
BshVI ATCGAT 1 cut(s) 793
BsiSI CCGG 1 cut(s) 44
BsmI GAATGC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 4 cut(s) 7, 124, 790, 794
BspCNI CTCAG 1 cut(s) 397
BspDI ATCGAT 1 cut(s) 793
BspLI GGNNCC 3 cut(s) 95, 175, 596
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 2, 132, 789
BspT107I GGYRCC 2 cut(s) 173, 594
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 2 cut(s) 451, 730
BssMI GATC 4 cut(s) 7, 124, 790, 794
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 6 cut(s) 25, 31, 51, 182, 230, 594
BstAUI TGTACA 1 cut(s) 478
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 4 cut(s) 61, 640, 650, 744
BstKTI GATC 4 cut(s) 10, 127, 793, 797
BstMBI GATC 4 cut(s) 7, 124, 790, 794
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 384, 475
Bsu15I ATCGAT 1 cut(s) 793
BsuTUI ATCGAT 1 cut(s) 793
BtsCI GGATG 4 cut(s) 61, 640, 650, 744
BtsIMutI CAGTG 3 cut(s) 27, 235, 390
BveI ACCTGC 1 cut(s) 89
ClaI ATCGAT 1 cut(s) 793
Csp6I GTAC 3 cut(s) 47, 479, 595
CviAII CATG 3 cut(s) 170, 683, 770
CviJI RGCY 6 cut(s) 295, 341, 375, 466, 667, 674
CviKI_1 RGCY 6 cut(s) 295, 341, 375, 466, 667, 674
CviQI GTAC 3 cut(s) 47, 479, 595
DdeI CTNAG 1 cut(s) 405
DpnI GATC 4 cut(s) 9, 126, 792, 796
DpnII GATC 4 cut(s) 7, 124, 790, 794
EcoRI GAATTC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 423
FaeI CATG 3 cut(s) 173, 686, 773
FatI CATG 3 cut(s) 169, 682, 769
Fnu4HI GCNGC 2 cut(s) 373, 464
FokI GGATG 4 cut(s) 68, 647, 657, 731
Fsp4HI GCNGC 2 cut(s) 373, 464
FspBI CTAG 3 cut(s) 135, 671, 675
GluI GCNGC 2 cut(s) 373, 464
GsuI CTGGAG 3 cut(s) 592, 645, 747
HapII CCGG 1 cut(s) 44
Hin1II CATG 3 cut(s) 173, 686, 773
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 339
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 7 cut(s) 7, 55, 115, 143, 188, 406, 519
Hpy188III TCNNGA 3 cut(s) 426, 609, 662
Hpy8I GTNNAC 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 635
HpyCH4III ACNGT 6 cut(s) 25, 31, 51, 182, 230, 594
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 9 cut(s) 84, 169, 225, 238, 421, 463, 686, 755, 779
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 3 cut(s) 173, 686, 773
KpnI GGTACC 1 cut(s) 598
Kzo9I GATC 4 cut(s) 7, 124, 790, 794
Lsp1109I GCAGC 2 cut(s) 384, 475
LweI GCATC 1 cut(s) 688
MaeI CTAG 3 cut(s) 135, 671, 675
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 25, 655
MalI GATC 4 cut(s) 9, 126, 792, 796
MboI GATC 4 cut(s) 7, 124, 790, 794
MboII GAAGA 3 cut(s) 502, 505, 516
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 8 cut(s) 239, 243, 307, 513, 739, 749, 774, 780
MmeI TCCRAC 2 cut(s) 33, 435
MnlI CCTC 3 cut(s) 310, 400, 631
Mph1103I ATGCAT 1 cut(s) 423
MseI TTAA 1 cut(s) 242
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 1 cut(s) 122
NdeII GATC 4 cut(s) 7, 124, 790, 794
NlaIII CATG 3 cut(s) 173, 686, 773
NlaIV GGNNCC 3 cut(s) 95, 175, 596
NmuCI GTSAC 2 cut(s) 25, 655
NsiI ATGCAT 1 cut(s) 423
NspI RCATGY 1 cut(s) 773
PaqCI CACCTGC 1 cut(s) 89
PciI ACATGT 1 cut(s) 769
PctI GAATGC 1 cut(s) 421
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 2 cut(s) 374, 465
PscI ACATGT 1 cut(s) 769
PshBI ATTAAT 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 95, 175, 596
RsaI GTAC 3 cut(s) 48, 480, 596
RsaNI GTAC 3 cut(s) 47, 479, 595
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 2 cut(s) 373, 464
Sau3AI GATC 4 cut(s) 7, 124, 790, 794
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 1 cut(s) 688
Sse9I AATT 8 cut(s) 239, 243, 307, 513, 739, 749, 774, 780
SspI AATATT 1 cut(s) 581
SspMI CTAG 3 cut(s) 135, 671, 675
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 6 cut(s) 25, 31, 51, 182, 230, 594
TaiI ACGT 1 cut(s) 353
TaqI TCGA 1 cut(s) 793
TasI AATT 8 cut(s) 239, 243, 307, 513, 739, 749, 774, 780
TatI WGTACW 1 cut(s) 478
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 3 cut(s) 34, 235, 397
TseFI GTSAC 2 cut(s) 25, 655
TseI GCWGC 2 cut(s) 372, 463
Tsp45I GTSAC 2 cut(s) 25, 655
TspDTI ATGAA 6 cut(s) 72, 279, 315, 447, 541, 549
TspGWI ACGGA 2 cut(s) 502, 555
TspRI CASTG 3 cut(s) 34, 235, 397
VspI ATTAAT 1 cut(s) 242
XapI RAATTY 2 cut(s) 513, 739
XceI RCATGY 1 cut(s) 773
XspI CTAG 3 cut(s) 135, 671, 675
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.