pycom15g11650

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008683
Physical Location & Seq
Reverse (-)
7897518 .. 7899631
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11650.2

Sequence Viewer

Length: 2031 bp
ATGTCCTATGAACTCAAAGAAGAAGGTAACAAGCTCTATCAGAAGCACGATCACGAAGGTGCGATGTTGAAGTATGAAAAAGCCCTCAAACTCCTTCCACAGAATCATTTTGAAGTTGCTCATCTGCATAGCAGTATGGCAGCATGTTATATACAAATGGGTATTGGGGAATATCCTCGAGCTATCAACGAATGTAATTTAGCGTTAGAGGTATCGCCGAAGTACAGTAAAGCACTGTTAAAAAGAGCATATTGTTATGAGGCTTTGAATAGGTTGGATTTGGCATTGAGAGATGTTAATGCTGTGCTGAGTATGGAACCGAATAATCTAAGTGCGTTGGAGATTTTGGCGAGTGTGAAGAGGGTGATGAATGAAAAGGGTATTGTTTATGATGAGAAAGAGATTGGTATTGCTAATGTACAGAAGCCTGCTGCTGCTCGTTTTCGTAAGGTGGTGAAAGAGAAGTTGAAGAAGAAGGGTAAGAAAGTTGAGCAGAAGATGGAGGATAAGGTCGTTGTGGAAGAGAAGGTGAGTGCTGATAAGGATAAAGAAGTGGTTACAAAGGCTGTTGAGGAAGATAAAGTGGTTACGAAGCACGTTGAGGAAGAGAAACTGGTTACAAAACATGTGGAGGAAGAAAAAGTAGTTGCAAGGCCAGTGAAGCTGGTGTTTGGAGAGGATATAAGGTGGGCACAACTGCCAGCGAATTGTAGCATGGGGTTGGCAAGGGAGATTGTTAGGGATCGATTTCCTGGCTTGAAGGGTGTTCTTGTTAAGTATAGAGATCAAGAGGGTGATTTGGTTACAATCACTACCACCGATGAGTTGAGGATAGCTGAATCTTCCTGTGATATGCAGGGCTCACTTAGATTATTCATATCAGAAGTCAGTCCTGATCAAGAACCAATTTACGAGGGATTGAGTGATGGGGAGGTGAGTATGGAAGACAAAAAACCAAGTAATGTTGTAGAGAATGGGGATACTGAAAACGATAGGGTAGTGGAAAAAAGGTCTACTTCTGTTGAGGATTGGGTTATCCAGTTTGCACGATTGTTCAAGAACCATGTTGGGTTCGATTCTGATTCGTATTTGGATCTTCATGAGCTTGGTGTGAAGCTATACTCAGAGGCAATGGAGGACACTGTAACACTTGAGGATGCTCAAGAACTTTTTGACATTGCTGCAGATAAGTTCCAAGAAATGGCGGCTTTGGCATTGTTTAATTGGGGAAATGTCCACATGTCTAGGGCAAGGAAACGAGTTTCCTTTCCTGAAAATGCTTCAAGGGAAACCATTACTGAACTGATCAAGGCTGGCTATGACTGGGCAAAGAAAGAGTATAAGAAGGCAGAGGGTAGGTACGAAGAGGCTGTGAAAATAAAACCCGACTTTTATGAAGGTTATCTTGCTCTGGGGCAACAACAGTTTGACCAAGCAAAGCTTTGTTGGTACTATGCTATTGGAAGCAAGAATGAGCTAGAAACAGGGCCTTCTTCGGAGGTGCTTCAGCTTTATAACAAGGCTGAGGACAGCATGGACAGGGGCATGCTGATGTGGGAGGAGATGGAAGAGCGGCGTCTAAATGGACTGTCCAAAGAAGAAAAATATAACACCCAATTGCAGAAATTGGGTTTGGATGGCCTAATTCAAGAGGTATCTGCTGATGAAGCTGCAGAGCATGCTGCAAATATGAAGTCACAGACATACCTCTTGTGGGGAACCTTGCTGTACGAGCGTTCCGTTGTGGAATATAAGTTAGGGCTACCTACTTGGGAGGAATGTTTAGAAGTTTCTGTTGAAAAGTTTGAACTTTGTGGAGCTTCTCCAACAGATATTGCCGTGATGATAAAGAACCACTGTTCCAATGAAACTGCATTGGAAGGTTTAGGGTTCAAAATTGATGAGATAATACAGGCATGGAATGAAATGTATGATGCTAAAAGGTGGCAATTTGGTGTTCCCTCTTTCCGGCTTGAGCCATTGTTGCGTCGGCAAGTTCCAAAACTTCATTCTATCTTGGAGCATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0008150 GO:0032886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

677

Amino Acids

77.25

Weight (kDa)

5.2

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccBSI CCGCTC 1 cut(s) 1573
AccI GTMKAC 1 cut(s) 1013
AciI CCGC 2 cut(s) 1205, 1573
AclWI GGATC 2 cut(s) 750, 1101
AcuI CTGAAG 1 cut(s) 1490
AcyI GRCGYC 1 cut(s) 1576
AfaI GTAC 5 cut(s) 224, 420, 1361, 1451, 1730
AfiI CCNNNNNNNGG 3 cut(s) 1201, 1714, 1968
AflIII ACRYGT 2 cut(s) 625, 1239
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 4 cut(s) 1616, 1648, 1993, 2025
AleI CACNNNNGTG 1 cut(s) 57
AloI GAACNNNNNNTCC 2 cut(s) 1844, 1876
AlwI GGATC 2 cut(s) 750, 1101
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 3 cut(s) 653, 1487, 1639
ApeKI GCWGC 6 cut(s) 140, 431, 434, 1181, 1670, 1682
ArsI GACNNNNNNTTYG 2 cut(s) 1679, 1711
AspS9I GGNCC 1 cut(s) 1487
AsuHPI GGTGA 5 cut(s) 376, 466, 541, 806, 946
AvaI CYCGRG 1 cut(s) 177
BaeGI GKGCMC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 951
BbvCI CCTCAGC 1 cut(s) 1524
BbvI GCAGC 6 cut(s) 152, 418, 421, 1168, 1657, 1669
BccI CCATC 4 cut(s) 493, 920, 1558, 1631
BceAI ACGGC 1 cut(s) 1823
BciT130I CCWGG 1 cut(s) 753
BciVI GTATCC 1 cut(s) 973
BclI TGATCA 2 cut(s) 895, 1305
BfaI CTAG 2 cut(s) 1245, 1478
BfmI CTRYAG 2 cut(s) 1182, 1671
BfuI GTATCC 1 cut(s) 973
BisI GCNGC 8 cut(s) 141, 432, 435, 1182, 1206, 1574, 1671, 1683
BlsI GCNGC 8 cut(s) 142, 433, 436, 1183, 1207, 1575, 1672, 1684
Bme1390I CCNGG 1 cut(s) 753
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 1487
BmiI GGNNCC 2 cut(s) 318, 1720
BmrFI CCNGG 1 cut(s) 753
BmrI ACTGGG 1 cut(s) 1333
BmsI GCATC 2 cut(s) 1147, 1924
BmuI ACTGGG 1 cut(s) 1333
BpiI GAAGAC 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 1524
BpuEI CTTGAG 3 cut(s) 1146, 1172, 1994
Bsa29I ATCGAT 1 cut(s) 745
BsaHI GRCGYC 1 cut(s) 1576
Bsc4I CCNNNNNNNGG 3 cut(s) 1201, 1714, 1968
Bse1I ACTGG 4 cut(s) 618, 656, 1039, 1328
Bse3DI GCAATG 2 cut(s) 1137, 1176
BseBI CCWGG 1 cut(s) 753
BseCI ATCGAT 1 cut(s) 745
BseGI GGATG 2 cut(s) 1162, 1642
BseLI CCNNNNNNNGG 3 cut(s) 1201, 1714, 1968
BseMI GCAATG 2 cut(s) 1137, 1176
BseMII CTCAG 3 cut(s) 299, 1137, 1515
BseNI ACTGG 4 cut(s) 618, 656, 1039, 1328
BseRI GAGGAG 1 cut(s) 1574
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 6 cut(s) 152, 418, 421, 1168, 1657, 1669
BshFI GGCC 3 cut(s) 655, 1489, 1641
BshVI ATCGAT 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 1969
BslI CCNNNNNNNGG 3 cut(s) 1201, 1714, 1968
BsnI GGCC 3 cut(s) 655, 1489, 1641
BsoBI CYCGRG 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 694, 863
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
BspACI CCGC 2 cut(s) 1205, 1573
BspANI GGCC 3 cut(s) 655, 1489, 1641
BspCNI CTCAG 3 cut(s) 300, 1136, 1516
BspDI ATCGAT 1 cut(s) 745
BspHI TCATGA 1 cut(s) 1099
BspLI GGNNCC 2 cut(s) 318, 1720
BspMAI CTGCAG 2 cut(s) 1186, 1675
BspPI GGATC 2 cut(s) 750, 1101
BspQI GCTCTTC 1 cut(s) 1563
BsrBI CCGCTC 1 cut(s) 1573
BsrDI GCAATG 2 cut(s) 1137, 1176
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 4 cut(s) 618, 656, 1039, 1328
BssMI GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
BssNI GRCGYC 1 cut(s) 1576
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 6 cut(s) 227, 237, 1144, 1425, 1590, 1859
Bst6I CTCTTC 5 cut(s) 353, 516, 600, 1359, 1563
BstACI GRCGYC 1 cut(s) 1576
BstAPI GCANNNNNTGC 1 cut(s) 1679
BstAUI TGTACA 1 cut(s) 418
BstC8I GCNNGC 6 cut(s) 429, 702, 1315, 1547, 1680, 2025
BstDEI CTNAG 5 cut(s) 308, 329, 866, 1123, 1524
BstF5I GGATG 2 cut(s) 1162, 1642
BstKTI GATC 6 cut(s) 52, 745, 787, 898, 1096, 1308
BstMBI GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
BstMWI GCNNNNNNNGC 6 cut(s) 661, 1211, 1667, 1679, 1732, 1984
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 6 cut(s) 147, 629, 1243, 1549, 1682, 2027
BstSCI CCNGG 1 cut(s) 751
BstSFI CTRYAG 2 cut(s) 1182, 1671
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 6 cut(s) 152, 418, 421, 1168, 1657, 1669
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 745
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 3 cut(s) 655, 1489, 1641
BsuTUI ATCGAT 1 cut(s) 745
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 2 cut(s) 1162, 1642
BtsIMutI CAGTG 4 cut(s) 233, 663, 1140, 1855
Cac8I GCNNGC 6 cut(s) 429, 702, 1315, 1547, 1680, 2025
CciI TCATGA 1 cut(s) 1099
Cfr13I GGNCC 1 cut(s) 1487
ClaI ATCGAT 1 cut(s) 745
CseI GACGC 2 cut(s) 1565, 1976
Csp6I GTAC 5 cut(s) 223, 419, 1360, 1450, 1729
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviQI GTAC 5 cut(s) 223, 419, 1360, 1450, 1729
DdeI CTNAG 5 cut(s) 308, 329, 866, 1123, 1524
DpnI GATC 6 cut(s) 51, 744, 786, 897, 1095, 1307
DpnII GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
Eam1104I CTCTTC 5 cut(s) 353, 516, 600, 1359, 1563
EarI CTCTTC 5 cut(s) 353, 516, 600, 1359, 1563
Eco24I GRGCYC 1 cut(s) 863
Eco57I CTGAAG 1 cut(s) 1490
Eco88I CYCGRG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 1487
EcoRII CCWGG 1 cut(s) 751
EcoT38I GRGCYC 1 cut(s) 863
FalI AAGNNNNNCTT 6 cut(s) 1000, 1032, 1389, 1421, 1425, 1457
FbaI TGATCA 2 cut(s) 895, 1305
FblI GTMKAC 1 cut(s) 1013
Fnu4HI GCNGC 8 cut(s) 141, 432, 435, 1182, 1206, 1574, 1671, 1683
FokI GGATG 2 cut(s) 1169, 1649
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 8 cut(s) 141, 432, 435, 1182, 1206, 1574, 1671, 1683
FspBI CTAG 2 cut(s) 1245, 1478
GluI GCNGC 8 cut(s) 141, 432, 435, 1182, 1206, 1574, 1671, 1683
HaeIII GGCC 3 cut(s) 655, 1489, 1641
HapII CCGG 1 cut(s) 1969
HgaI GACGC 2 cut(s) 1565, 1976
Hin1I GRCGYC 1 cut(s) 1576
HindIII AAGCTT 1 cut(s) 1439
HinfI GANTC 4 cut(s) 103, 839, 1076, 1082
HpaII CCGG 1 cut(s) 1969
HphI GGTGA 5 cut(s) 376, 466, 541, 806, 946
Hpy166II GTNNAC 2 cut(s) 1014, 1237
Hpy188I TCNGA 5 cut(s) 42, 883, 1081, 1126, 1498
Hpy188III TCNNGA 9 cut(s) 53, 788, 893, 899, 1057, 1100, 1163, 1271, 1649
Hpy8I GTNNAC 2 cut(s) 1014, 1237
Hpy99I CGWCG 1 cut(s) 1992
HpyCH4III ACNGT 6 cut(s) 227, 237, 1144, 1425, 1590, 1859
HpyCH4IV ACGT 1 cut(s) 597
HpyCH4V TGCA 9 cut(s) 127, 650, 856, 1046, 1184, 1621, 1673, 1685, 1874
HpyF10VI GCNNNNNNNGC 6 cut(s) 661, 1211, 1667, 1679, 1732, 1984
HpyF3I CTNAG 5 cut(s) 308, 329, 866, 1123, 1524
HpySE526I ACGT 1 cut(s) 597
Hsp92I GRCGYC 1 cut(s) 1576
Ksp22I TGATCA 2 cut(s) 895, 1305
Kzo9I GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
LguI GCTCTTC 1 cut(s) 1563
LmnI GCTCC 2 cut(s) 1817, 2020
Lsp1109I GCAGC 6 cut(s) 152, 418, 421, 1168, 1657, 1669
LweI GCATC 2 cut(s) 1147, 1924
MaeI CTAG 2 cut(s) 1245, 1478
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 7 cut(s) 26, 556, 586, 616, 802, 1144, 1695
MalI GATC 6 cut(s) 51, 744, 786, 897, 1095, 1307
MbiI CCGCTC 1 cut(s) 1573
MboI GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
MfeI CAATTG 1 cut(s) 1616
MflI RGATCY 1 cut(s) 1093
MhlI GDGCHC 2 cut(s) 694, 863
MluCI AATT 9 cut(s) 196, 706, 906, 1222, 1616, 1625, 1644, 1896, 1949
MmeI TCCRAC 3 cut(s) 255, 318, 1850
MseI TTAA 4 cut(s) 239, 297, 774, 1221
MslI CAYNNNNRTG 2 cut(s) 57, 1550
MspI CCGG 1 cut(s) 1969
MspR9I CCNGG 1 cut(s) 753
MunI CAATTG 1 cut(s) 1616
MvaI CCWGG 1 cut(s) 753
MwoI GCNNNNNNNGC 6 cut(s) 661, 1211, 1667, 1679, 1732, 1984
NdeII GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
NlaIV GGNNCC 2 cut(s) 318, 1720
NmuCI GTSAC 1 cut(s) 1695
NspI RCATGY 6 cut(s) 147, 629, 1243, 1549, 1682, 2027
OliI CACNNNNGTG 1 cut(s) 57
PaeI GCATGC 3 cut(s) 1549, 1682, 2027
PaeR7I CTCGAG 1 cut(s) 177
PagI TCATGA 1 cut(s) 1099
PciI ACATGT 2 cut(s) 625, 1239
PciSI GCTCTTC 1 cut(s) 1563
PcsI WCGNNNNNNNCGW 1 cut(s) 1737
PfeI GAWTC 4 cut(s) 103, 839, 1076, 1082
PflMI CCANNNNNTGG 1 cut(s) 1201
PkrI GCNGC 8 cut(s) 142, 433, 436, 1183, 1207, 1575, 1672, 1684
PscI ACATGT 2 cut(s) 625, 1239
PsiI TTATAA 1 cut(s) 1515
Psp6I CCWGG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 2 cut(s) 318, 1720
PspPI GGNCC 1 cut(s) 1487
PspXI VCTCGAGB 1 cut(s) 177
PstI CTGCAG 2 cut(s) 1186, 1675
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 5 cut(s) 224, 420, 1361, 1451, 1730
RsaNI GTAC 5 cut(s) 223, 419, 1360, 1450, 1729
RseI CAYNNNNRTG 2 cut(s) 57, 1550
SapI GCTCTTC 1 cut(s) 1563
SaqAI TTAA 4 cut(s) 239, 297, 774, 1221
SatI GCNGC 8 cut(s) 141, 432, 435, 1182, 1206, 1574, 1671, 1683
Sau3AI GATC 6 cut(s) 49, 742, 784, 895, 1093, 1305
Sau96I GGNCC 1 cut(s) 1487
ScrFI CCNGG 1 cut(s) 753
SduI GDGCHC 2 cut(s) 694, 863
SfaNI GCATC 2 cut(s) 1147, 1924
SfcI CTRYAG 2 cut(s) 1182, 1671
Sfr274I CTCGAG 1 cut(s) 177
SlaI CTCGAG 1 cut(s) 177
SmiMI CAYNNNNRTG 2 cut(s) 57, 1550
SmlI CTYRAG 4 cut(s) 177, 1151, 1161, 1973
SmoI CTYRAG 4 cut(s) 177, 1151, 1161, 1973
SphI GCATGC 3 cut(s) 1549, 1682, 2027
Sse9I AATT 9 cut(s) 196, 706, 906, 1222, 1616, 1625, 1644, 1896, 1949
SsiI CCGC 2 cut(s) 1205, 1573
SspMI CTAG 2 cut(s) 1245, 1478
StyD4I CCNGG 1 cut(s) 751
TaaI ACNGT 6 cut(s) 227, 237, 1144, 1425, 1590, 1859
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 178, 745, 1074
TasI AATT 9 cut(s) 196, 706, 906, 1222, 1616, 1625, 1644, 1896, 1949
TatI WGTACW 2 cut(s) 222, 418
TauI GCSGC 2 cut(s) 1208, 1576
TfiI GAWTC 4 cut(s) 103, 839, 1076, 1082
Tru1I TTAA 4 cut(s) 239, 297, 774, 1221
Tru9I TTAA 4 cut(s) 239, 297, 774, 1221
TscAI CASTG 4 cut(s) 240, 663, 1147, 1862
TseFI GTSAC 1 cut(s) 1695
TseI GCWGC 6 cut(s) 140, 431, 434, 1181, 1670, 1682
Tsp45I GTSAC 1 cut(s) 1695
TspGWI ACGGA 1 cut(s) 1729
TspRI CASTG 4 cut(s) 240, 663, 1147, 1862
Van91I CCANNNNNTGG 1 cut(s) 1201
XceI RCATGY 6 cut(s) 147, 629, 1243, 1549, 1682, 2027
XhoI CTCGAG 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 1013
XspI CTAG 2 cut(s) 1245, 1478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.