Rmu_sc0004989.1_g000001

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004989.1
OG0008683
Physical Location & Seq
Forward (+)
1105 .. 7158
6054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004989.1_g000001.1.cds

Sequence Viewer

Length: 2478 bp
atgggaaagccaactgggaagaagaaggaccatgatgtgcagaagcctgggaatgctgcgagtaagcagagcaaagctgcggatcgaaactcgaaagccttggatgaagacactgcaatcttcatcaacatgtcccaagagctcagagaagaaggtaacaagctcttccagaagcgtgacagtgaaggcgcaatgttgaagtatgagaaagcgctcaaactactgcccaagaaccatattgatgttgctcatttacatagtaacatggctgcttgttatatgcaaatgggtctcggtgaatatcctcgggcaatcaacgaatgcaatttggcattagaggtgtcaccgaggtacagtaaagcactactgagaaggtcgaggtgttatgaggctttgaataggttggatttggcattgagggatgttaataccgttttgagtatggagccgaataatctaaatgcattggagattttggagagcgtcaagacggtgatgagcgaaaaggggattgtttttgatgagaaagaaattggtattgctaatgtacagcagatccctgctgctcgttttcgtaaagtggtgaaagagaagctaaagaagaagaagggtaagagggttgaggagaagacagaggacaaggctgttgtggaagaaaatgtgagagttattaaggacaaagaagtggtaacaaagactgttgaggaagagaaagcagtcgtaaagcatgttgaggaagagaaacaagtcatgaatcatgtggaagagaagcatattaaggaggaaaccatagttactaagacagtcaagttggtgtttggagaggatataagatgggcacagtttccactgagttgtagcgtgaggctgctcagggagatagttagggatcgatttcctggcttgaagggtgttctggtgaagtatagagatcaagagggtgacttggttacaatcactactacggatgagttgaggttggctgaatcatcatgtgatatgaggggatcacttagattttttataacagaagtcggtgctgatcaagaaccaatttatgagggatcaagtgaagaggaagtgcgtaaggaagatccaaagccaagtaatgttgttgagaatggggacagtgggaatcacacagaagtaggaaaggggtctacgacttgtgttgaggactggattatccagtttgcaagattgttcaagaaccatgttgggttcgattctgattcatacttggatcttcacgaacttggggtgaagctatactctgaggcaatggaggacactgtaacacttgatgatgctcaagaactttttgacattgctgcaattaagttccaagagatggcggctttggcattgttcaattggggaaatgtccacatgtctaaggcaaggaagcgggcatccttgccagaagatgcttcaagggaaaccgttattgaacagattaaggctgtatatgaatgggcacagaaagagtataagaaggcagagagtaggtatgaagaagctgtgaaaataaagccagacttctacgaaggttatcttgcactcggccaacaacagtttgaacaagcaaagctttcttggtactatgcgattggaagcaagattgagttacaaactgagccttcttcagaggtgcttcagctttataacaaggctgaggacagcatggacaagggaatgctgttgtgggaggagatggaagagcggcgtctaaatggactctccaaagaagataagtataaagcccaattgcagaaaatgggattggatggcctatttgaggaggtgtctgctgatgaagctgcagaacaggctgcaaatatgaagtcacaaatatacctcctgtggggaaccttgctgtatgagcgttctgttgtggaatataagctagagctaccaacttgggaggaatgtttagaagttgccattgagaagtttgaactttgtggagcgtctccaacagatatagctgtgatgataaagaatcactgttccaatgaaactgcagtggaaggtatggggttcaaaattgatgagataatacaggcgtggaatgaaatgtatgatgctaagagttttccttattcaaaagtttgcaggctgcatggctgcacaggtattttagtttttgtcaatggactgttgtgtgcgtcttcttgtttgctttgggagcaacaaagggaagacatggtgttcaatatgttagagttgggggcactagaggaggaaaatgccaatttgttgctccaacaagaaaaagaggacaatttgaaagctttattggaaggtcacaatctacaagtttcggtactaaaggaggaggacaaacaattgcaaatgccagaagaggaagaagccaattcattgctccaacaagaggaaaatggcaacctacaagctttggtaatggtggaggacaagctcttccaagtattggaagtggaaggggacaatatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0008150 GO:0032886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

825

Amino Acids

94.07

Weight (kDa)

4.94

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1025, 1665
AccB7I CCANNNNNTGG 2 cut(s) 1351, 2452
AccBSI CCGCTC 1 cut(s) 1723
AccI GTMKAC 1 cut(s) 1160
AciI CCGC 4 cut(s) 80, 1355, 1408, 1723
AclWI GGATC 7 cut(s) 90, 550, 897, 1015, 1072, 1088, 1251
AcoI YGGCCR 1 cut(s) 1564
AcuI CTGAAG 2 cut(s) 1629, 1640
AcyI GRCGYC 1 cut(s) 1726
AfaI GTAC 4 cut(s) 353, 549, 1601, 2328
AfeI AGCGCT 1 cut(s) 213
AfiI CCNNNNNNNGG 5 cut(s) 1351, 1798, 1864, 2395, 2452
AflIII ACRYGT 2 cut(s) 129, 1389
AjnI CCWGG 2 cut(s) 46, 898
AjuI GAANNNNNNNTTGG 4 cut(s) 1766, 1798, 2282, 2314
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 296, 1977
AlwI GGATC 7 cut(s) 90, 550, 897, 1015, 1072, 1088, 1251
Ama87I CYCGRG 1 cut(s) 306
Aor51HI AGCGCT 1 cut(s) 213
AoxI GGCC 2 cut(s) 1564, 1789
ArsI GACNNNNNNTTYG 2 cut(s) 1829, 1861
AspLEI GCGC 2 cut(s) 191, 214
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 7 cut(s) 308, 336, 505, 595, 931, 953, 1273
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 3 cut(s) 841, 1480, 2236
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 4 cut(s) 114, 635, 2163, 2208
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 1674
BccI CCATC 4 cut(s) 828, 1345, 1708, 1781
BciT130I CCWGG 2 cut(s) 48, 900
BclI TGATCA 1 cut(s) 1042
BcoDI GTCTC 2 cut(s) 296, 1977
BfaI CTAG 2 cut(s) 1907, 2237
BfmI CTRYAG 2 cut(s) 1821, 2022
BfoI RGCGCY 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 48, 900
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 447, 1870
BmrFI CCNGG 2 cut(s) 48, 900
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 4 cut(s) 1297, 1417, 1421, 2074
BmuI ACTGGG 1 cut(s) 24
BpiI GAAGAC 4 cut(s) 114, 635, 2163, 2208
Bpu10I CCTNAGC 2 cut(s) 872, 1674
BpuEI CTTGAG 1 cut(s) 1296
Bsa29I ATCGAT 1 cut(s) 892
BsaHI GRCGYC 1 cut(s) 1726
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 47, 99, 305, 347
Bsc4I CCNNNNNNNGG 5 cut(s) 1351, 1798, 1864, 2395, 2452
Bse1I ACTGG 3 cut(s) 19, 1184, 1189
Bse3DI GCAATG 4 cut(s) 198, 1287, 1326, 2381
BseBI CCWGG 2 cut(s) 48, 900
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 4 cut(s) 47, 99, 305, 347
BseGI GGATG 5 cut(s) 109, 427, 973, 1412, 1792
BseLI CCNNNNNNNGG 5 cut(s) 1351, 1798, 1864, 2395, 2452
BseMI GCAATG 4 cut(s) 198, 1287, 1326, 2381
BseMII CTCAG 7 cut(s) 157, 359, 842, 886, 1266, 1626, 1665
BseNI ACTGG 3 cut(s) 19, 1184, 1189
BseRI GAGGAG 5 cut(s) 638, 1724, 1814, 2255, 2351
BseSI GKGCMC 3 cut(s) 841, 1480, 2236
BsgI GTGCAG 2 cut(s) 59, 2113
BshFI GGCC 2 cut(s) 1566, 1791
BshVI ATCGAT 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 306
BslFI GGGAC 2 cut(s) 118, 1139
BslI CCNNNNNNNGG 5 cut(s) 1351, 1798, 1864, 2395, 2452
BsmAI GTCTC 2 cut(s) 296, 1977
BsmBI CGTCTC 1 cut(s) 1977
BsmFI GGGAC 2 cut(s) 118, 1139
BsmI GAATGC 3 cut(s) 58, 326, 1701
BsnI GGCC 2 cut(s) 1566, 1791
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 4 cut(s) 144, 841, 1480, 2236
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
BspACI CCGC 4 cut(s) 80, 1355, 1408, 1723
BspANI GGCC 2 cut(s) 1566, 1791
BspCNI CTCAG 7 cut(s) 156, 360, 843, 885, 1267, 1627, 1666
BspDI ATCGAT 1 cut(s) 892
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 2 cut(s) 447, 1870
BspMAI CTGCAG 2 cut(s) 1825, 2026
BspPI GGATC 7 cut(s) 90, 550, 897, 1015, 1072, 1088, 1251
BspQI GCTCTTC 3 cut(s) 170, 1713, 2447
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 1723
BsrDI GCAATG 4 cut(s) 198, 1287, 1326, 2381
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 3 cut(s) 19, 1184, 1189
BssECI CCNNGG 4 cut(s) 47, 99, 305, 347
BssMI GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
BssNI GRCGYC 1 cut(s) 1726
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 48, 900
Bst6I CTCTTC 8 cut(s) 170, 702, 732, 759, 1068, 1713, 2358, 2447
BstACI GRCGYC 1 cut(s) 1726
BstAUI TGTACA 1 cut(s) 547
BstC8I GCNNGC 2 cut(s) 1410, 2117
BstENI CCTNNNNNAGG 1 cut(s) 1796
BstF5I GGATG 5 cut(s) 109, 427, 973, 1412, 1792
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 191, 214
BstKTI GATC 9 cut(s) 85, 558, 892, 934, 1010, 1045, 1067, 1096, 1246
BstMAI GTCTC 2 cut(s) 296, 1977
BstMBI GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
BstMWI GCNNNNNNNGC 5 cut(s) 1361, 1817, 1829, 1882, 2188
BstNI CCWGG 2 cut(s) 48, 900
BstNSI RCATGY 3 cut(s) 133, 731, 1393
BstSCI CCNGG 2 cut(s) 46, 898
BstSFI CTRYAG 2 cut(s) 1821, 2022
BstSLI GKGCMC 3 cut(s) 841, 1480, 2236
BstV2I GAAGAC 4 cut(s) 114, 635, 2163, 2208
BstX2I RGATCY 3 cut(s) 555, 1093, 1243
BstYI RGATCY 3 cut(s) 555, 1093, 1243
Bsu15I ATCGAT 1 cut(s) 892
BsuRI GGCC 2 cut(s) 1566, 1791
BsuTUI ATCGAT 1 cut(s) 892
BtsCI GGATG 5 cut(s) 109, 427, 973, 1412, 1792
BtsI GCAGTG 2 cut(s) 111, 2031
BtsIMutI CAGTG 7 cut(s) 111, 187, 848, 1135, 1290, 2005, 2031
Cac8I GCNNGC 2 cut(s) 1410, 2117
CciI TCATGA 1 cut(s) 750
CfoI GCGC 2 cut(s) 191, 214
Cfr13I GGNCC 1 cut(s) 28
ClaI ATCGAT 1 cut(s) 892
CseI GACGC 4 cut(s) 472, 1715, 1959, 2157
Csp6I GTAC 4 cut(s) 352, 548, 1600, 2327
CviQI GTAC 4 cut(s) 352, 548, 1600, 2327
DpnI GATC 9 cut(s) 84, 557, 891, 933, 1009, 1044, 1066, 1095, 1245
DpnII GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
EaeI YGGCCR 1 cut(s) 1564
Eam1104I CTCTTC 8 cut(s) 170, 702, 732, 759, 1068, 1713, 2358, 2447
EarI CTCTTC 8 cut(s) 170, 702, 732, 759, 1068, 1713, 2358, 2447
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 28
Eco47III AGCGCT 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 2 cut(s) 1629, 1640
Eco88I CYCGRG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 142
EcoNI CCTNNNNNAGG 1 cut(s) 1796
EcoRII CCWGG 2 cut(s) 46, 898
EcoT14I CCWWGG 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 1977
FalI AAGNNNNNCTT 6 cut(s) 1523, 1555, 1539, 1571, 1575, 1607
FaqI GGGAC 2 cut(s) 118, 1139
FauI CCCGC 1 cut(s) 1401
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 1160
FokI GGATG 5 cut(s) 116, 434, 980, 1399, 1799
FriOI GRGCYC 1 cut(s) 144
FspBI CTAG 2 cut(s) 1907, 2237
GlaI GCGC 2 cut(s) 190, 213
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 2 cut(s) 1566, 1791
HgaI GACGC 4 cut(s) 472, 1715, 1959, 2157
HhaI GCGC 2 cut(s) 191, 214
Hin1I GRCGYC 1 cut(s) 1726
Hin6I GCGC 2 cut(s) 189, 212
HinP1I GCGC 2 cut(s) 189, 212
HindIII AAGCTT 3 cut(s) 1589, 2292, 2415
HinfI GANTC 7 cut(s) 754, 986, 1135, 1226, 1232, 1737, 2002
HphI GGTGA 7 cut(s) 308, 336, 505, 595, 931, 953, 1273
Hpy166II GTNNAC 2 cut(s) 1161, 1387
Hpy188I TCNGA 4 cut(s) 146, 1231, 1276, 1648
Hpy188III TCNNGA 8 cut(s) 169, 487, 751, 935, 1046, 1207, 1250, 1313
Hpy8I GTNNAC 2 cut(s) 1161, 1387
HpyF10VI GCNNNNNNNGC 5 cut(s) 1361, 1817, 1829, 1882, 2188
Hsp92I GRCGYC 1 cut(s) 1726
HspAI GCGC 2 cut(s) 189, 212
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
LguI GCTCTTC 3 cut(s) 170, 1713, 2447
LmnI GCTCC 5 cut(s) 445, 1967, 2188, 2268, 2391
LweI GCATC 4 cut(s) 1297, 1417, 1421, 2074
MaeI CTAG 2 cut(s) 1907, 2237
MalI GATC 9 cut(s) 84, 557, 891, 933, 1009, 1044, 1066, 1095, 1245
MbiI CCGCTC 1 cut(s) 1723
MboI GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
MfeI CAATTG 3 cut(s) 1372, 1766, 2348
MflI RGATCY 3 cut(s) 555, 1093, 1243
MhlI GDGCHC 4 cut(s) 144, 841, 1480, 2236
MlyI GAGTC 1 cut(s) 1731
MmeI TCCRAC 4 cut(s) 384, 2000, 2290, 2413
Mph1103I ATGCAT 1 cut(s) 466
MseI TTAA 5 cut(s) 426, 672, 777, 1338, 1458
MslI CAYNNNNRTG 2 cut(s) 128, 240
MspR9I CCNGG 2 cut(s) 48, 900
MunI CAATTG 3 cut(s) 1372, 1766, 2348
Mva1269I GAATGC 3 cut(s) 58, 326, 1701
MvaI CCWGG 2 cut(s) 48, 900
MwoI GCNNNNNNNGC 5 cut(s) 1361, 1817, 1829, 1882, 2188
NdeII GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
NlaIV GGNNCC 2 cut(s) 447, 1870
NmeAIII GCCGAG 1 cut(s) 1542
NmuCI GTSAC 5 cut(s) 176, 342, 941, 1845, 2306
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 3 cut(s) 133, 731, 1393
PagI TCATGA 1 cut(s) 750
PciI ACATGT 2 cut(s) 129, 1389
PciSI GCTCTTC 3 cut(s) 170, 1713, 2447
PctI GAATGC 3 cut(s) 58, 326, 1701
PfeI GAWTC 6 cut(s) 754, 986, 1135, 1226, 1232, 2002
PflMI CCANNNNNTGG 2 cut(s) 1351, 2452
PleI GAGTC 1 cut(s) 1731
PpsI GAGTC 1 cut(s) 1731
PscI ACATGT 2 cut(s) 129, 1389
PsiI TTATAA 2 cut(s) 1025, 1665
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 46, 898
PspGI CCWGG 2 cut(s) 46, 898
PspN4I GGNNCC 2 cut(s) 447, 1870
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 1825, 2026
PsuI RGATCY 3 cut(s) 555, 1093, 1243
RsaI GTAC 4 cut(s) 353, 549, 1601, 2328
RsaNI GTAC 4 cut(s) 352, 548, 1600, 2327
RseI CAYNNNNRTG 2 cut(s) 128, 240
SacI GAGCTC 1 cut(s) 144
SapI GCTCTTC 3 cut(s) 170, 1713, 2447
SaqAI TTAA 5 cut(s) 426, 672, 777, 1338, 1458
Sau3AI GATC 9 cut(s) 82, 555, 889, 931, 1007, 1042, 1064, 1093, 1243
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 1731
ScrFI CCNGG 2 cut(s) 48, 900
SduI GDGCHC 4 cut(s) 144, 841, 1480, 2236
SfaNI GCATC 4 cut(s) 1297, 1417, 1421, 2074
SfcI CTRYAG 2 cut(s) 1821, 2022
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 128, 240
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SsiI CCGC 4 cut(s) 80, 1355, 1408, 1723
SspMI CTAG 2 cut(s) 1907, 2237
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 46, 898
StyI CCWWGG 1 cut(s) 99
TaqI TCGA 5 cut(s) 85, 92, 377, 892, 1224
TatI WGTACW 1 cut(s) 547
TauI GCSGC 2 cut(s) 1358, 1726
TfiI GAWTC 6 cut(s) 754, 986, 1135, 1226, 1232, 2002
Tru1I TTAA 5 cut(s) 426, 672, 777, 1338, 1458
Tru9I TTAA 5 cut(s) 426, 672, 777, 1338, 1458
TscAI CASTG 7 cut(s) 118, 187, 855, 1135, 1297, 2012, 2031
TseFI GTSAC 5 cut(s) 176, 342, 941, 1845, 2306
Tsp45I GTSAC 5 cut(s) 176, 342, 941, 1845, 2306
TspGWI ACGGA 1 cut(s) 980
TspRI CASTG 7 cut(s) 118, 187, 855, 1135, 1297, 2012, 2031
Van91I CCANNNNNTGG 2 cut(s) 1351, 2452
VpaK11BI GGWCC 1 cut(s) 28
XagI CCTNNNNNAGG 1 cut(s) 1796
XceI RCATGY 3 cut(s) 133, 731, 1393
XmiI GTMKAC 1 cut(s) 1160
XspI CTAG 2 cut(s) 1907, 2237
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.