pycom15g11750

hydroxyproline O-arabinosyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013842
Physical Location & Seq
Reverse (-)
7957380 .. 7959689
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1092 bp
ATGGGTTGTGGAAATTTCTTCTTCACCATACTGGTGACCTTCTCCGTAGCTCTAATCACGTATAACATCATAATCTCAGCCAATTCGCCACTCCAGCAGGACCTTCCAGGTCCTTCGAGATCATCATTGTCGATTGGTGTGGACCCCATCATCCAGATGCCATTTCACAGATCGCGAGGAAAATCGGGGAGATCCAAGCGACTGTTCCATACGGCGGTGACGGCCTCCGACTCCGTCTACAACACGTGGCAGTGCAGGATCATGTATTACTGGTTCAAGAAGTTTCAGAACGAACCCGGTTCGGAGATGGGCGGGTTCACTAGGATCTTGCATAATGGGAAGCCGGACAAGTACATGGATGAAATCCCAACTTTTATTGCCCAGCCTTTGCCTGCTGGAATGGATCGGGGTTATATAGTTCTCAACAGACCATGGGCATTTGTGCAATGGCTTCAGCAAGCAGACATCAAAGAAGATTATATACTGATGTCGGAGCCTGATCATGTGATTGTCAAGCCCATACCAAACTTGTCCACTGACGGGCTTGGAGTCGCGTTTCCTTTCTTCTACATTGAACCGAAAAGGTACGAGTCAGTGCTCCGGAAATACTTCCCTGAGAACAAGGGACCAATAACTAACATTGATCCAATTGGAAATTCGCCCGTCATTGTTGGGAAGGAATCTCTTAAGAAGATTGCTCCGACCTGGATGAATGTTTCTTTGGCAATGAAGAAGGACCCTGAAGCAGACAAAGCTTTTGGATGGGTGCTTGAAATGTATGCTTATGCTGTTGCATCCGCTCACCATGGTGTTGGTAATATCTTGTACAAGGATTTCATGCTTCAGCCTCCATGGGATAAAGAAATTGGCAAGAAGTTCATAATTCATTTCACTTATGGATGCGACTATAATATGAAGGGTGAACTAACATATGGGAAAATTGGAGAATGGAGATTTGACAAACGATCTTATGGTACTGTTGCACCGCCTAGAAACCTCCCTATGCCTCCACATGGTGTTCCAGAAAGCGTGGTGACGCTGGTGAAAATGGTGAATGAAGCGACAGCAAACATTCCGAATTGGGGGGAATAG

Protein Analysis

364

Amino Acids

41.15

Weight (kDa)

8.85

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 69 - 361 2.9e-128 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25265
fragaria_vesca FvH4_2g28340
malus_domestica MD08G1153200.v1.1 MD15G1130300.v1.1
prunus_persica Prupe.1G485800_v2.0.a1
pyrus_communis pycom08g13260 pycom15g11750
rosa_chinensis RchiOBHm_Chr6g0297261
rosa_laevigata RLG00000011616
rosa_multiflora Rmu_sc0004535.1_g000002 Rmu_sc0007115.1_g000001
rosa_roxburghii Rroxscaffold_7G00170700
rosa_rugosa Rorug06G0270100
rosa_samantha Rh6BG389600 Rh6CG394800 Rh6DG381500
rosa_wichuraiana Rw6G033240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 800
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 175, 554
AccIII TCCGGA 1 cut(s) 600
AciI CCGC 4 cut(s) 215, 312, 798, 986
AclWI GGATC 5 cut(s) 186, 266, 332, 411, 638
AcsI RAATTY 2 cut(s) 13, 655
AcuI CTGAAG 3 cut(s) 437, 762, 827
AcvI CACGTG 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 4 cut(s) 353, 587, 827, 976
AfiI CCNNNNNNNGG 4 cut(s) 214, 540, 1013, 1082
AflII CTTAAG 1 cut(s) 686
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 3 cut(s) 277, 575, 773
AjnI CCWGG 2 cut(s) 106, 704
AjuI GAANNNNNNNTTGG 6 cut(s) 188, 220, 640, 672, 704, 736
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 2 cut(s) 50, 755
AluI AGCT 2 cut(s) 50, 755
Alw21I GWGCWC 1 cut(s) 600
AlwI GGATC 5 cut(s) 186, 266, 332, 411, 638
Aor13HI TCCGGA 1 cut(s) 600
AoxI GGCC 1 cut(s) 222
ApoI RAATTY 2 cut(s) 13, 655
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
Asp700I GAANNNNTTC 1 cut(s) 608
AspS9I GGNCC 5 cut(s) 100, 110, 142, 626, 736
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 8 cut(s) 16, 46, 229, 794, 932, 1045, 1054, 1063
AvaII GGWCC 5 cut(s) 100, 110, 142, 626, 736
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbrPI CACGTG 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 600
BccI CCATC 3 cut(s) 155, 301, 756
BceAI ACGGC 2 cut(s) 228, 237
BciT130I CCWGG 2 cut(s) 108, 706
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 1 cut(s) 297
BfaI CTAG 2 cut(s) 321, 990
BfrI CTTAAG 1 cut(s) 686
Bme1390I CCNGG 3 cut(s) 108, 297, 706
Bme18I GGWCC 5 cut(s) 100, 110, 142, 626, 736
BmgT120I GGNCC 5 cut(s) 100, 110, 142, 626, 736
BmiI GGNNCC 4 cut(s) 144, 495, 627, 738
BmrFI CCNGG 3 cut(s) 108, 297, 706
BmsI GCATC 3 cut(s) 147, 803, 890
BpmI CTGGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 2 cut(s) 60, 246
BsaJI CCNNGG 3 cut(s) 431, 805, 851
BsaWI WCCGGW 1 cut(s) 600
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 540, 1013, 1082
Bse1I ACTGG 2 cut(s) 36, 275
Bse3DI GCAATG 2 cut(s) 452, 732
BseAI TCCGGA 1 cut(s) 600
BseBI CCWGG 2 cut(s) 108, 706
BseDI CCNNGG 3 cut(s) 431, 805, 851
BseGI GGATG 6 cut(s) 150, 364, 714, 767, 794, 905
BseLI CCNNNNNNNGG 4 cut(s) 214, 540, 1013, 1082
BseMI GCAATG 2 cut(s) 452, 732
BseMII CTCAG 2 cut(s) 90, 606
BseNI ACTGG 2 cut(s) 36, 275
BseYI CCCAGC 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 2 cut(s) 175, 554
BshFI GGCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 600
BsiSI CCGG 3 cut(s) 297, 344, 601
BslFI GGGAC 1 cut(s) 639
BslI CCNNNNNNNGG 4 cut(s) 214, 540, 1013, 1082
BsmFI GGGAC 1 cut(s) 639
BsnI GGCC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 600
Bsp13I TCCGGA 1 cut(s) 600
Bsp1407I TGTACA 1 cut(s) 825
Bsp143I GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
Bsp19I CCATGG 3 cut(s) 431, 805, 851
Bsp68I TCGCGA 1 cut(s) 175
BspACI CCGC 4 cut(s) 215, 312, 798, 986
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 2 cut(s) 89, 607
BspEI TCCGGA 1 cut(s) 600
BspFNI CGCG 2 cut(s) 175, 554
BspLI GGNNCC 4 cut(s) 144, 495, 627, 738
BspPI GGATC 5 cut(s) 186, 266, 332, 411, 638
BspTI CTTAAG 1 cut(s) 686
BsrBI CCGCTC 1 cut(s) 800
BsrDI GCAATG 2 cut(s) 452, 732
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 2 cut(s) 36, 275
BssECI CCNNGG 3 cut(s) 431, 805, 851
BssMI GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
BssT1I CCWWGG 3 cut(s) 431, 805, 851
Bst2UI CCWGG 2 cut(s) 108, 706
Bst4CI ACNGT 2 cut(s) 204, 979
BstAFI CTTAAG 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 825
BstBAI YACGTR 2 cut(s) 60, 246
BstC8I GCNNGC 2 cut(s) 393, 459
BstDEI CTNAG 2 cut(s) 76, 615
BstDSI CCRYGG 3 cut(s) 431, 805, 851
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 6 cut(s) 150, 364, 714, 767, 794, 905
BstFNI CGCG 2 cut(s) 175, 554
BstKTI GATC 9 cut(s) 122, 173, 194, 261, 327, 406, 502, 646, 968
BstMBI GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
BstMWI GCNNNNNNNGC 3 cut(s) 94, 221, 752
BstNI CCWGG 2 cut(s) 108, 706
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 3 cut(s) 106, 295, 704
BstUI CGCG 2 cut(s) 175, 554
BstX2I RGATCY 2 cut(s) 191, 324
BstXI CCANNNNNNTGG 1 cut(s) 812
BstYI RGATCY 2 cut(s) 191, 324
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 3 cut(s) 431, 805, 851
BtsCI GGATG 6 cut(s) 150, 364, 714, 767, 794, 905
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 3 cut(s) 257, 534, 600
BtuMI TCGCGA 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 393, 459
Cfr13I GGNCC 5 cut(s) 100, 110, 142, 626, 736
CseI GACGC 1 cut(s) 1045
Csp6I GTAC 4 cut(s) 352, 586, 826, 975
CviAII CATG 8 cut(s) 262, 355, 432, 503, 806, 838, 852, 1013
CviQI GTAC 4 cut(s) 352, 586, 826, 975
DdeI CTNAG 2 cut(s) 76, 615
DpnI GATC 9 cut(s) 121, 172, 193, 260, 326, 405, 501, 645, 967
DpnII GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
DraIII CACNNNGTG 1 cut(s) 1016
Eco130I CCWWGG 3 cut(s) 431, 805, 851
Eco47I GGWCC 5 cut(s) 100, 110, 142, 626, 736
Eco57I CTGAAG 3 cut(s) 437, 762, 827
Eco72I CACGTG 1 cut(s) 246
Eco91I GGTNACC 1 cut(s) 34
EcoO109I RGGNCCY 3 cut(s) 100, 110, 736
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 106, 704
EcoT14I CCWWGG 3 cut(s) 431, 805, 851
ErhI CCWWGG 3 cut(s) 431, 805, 851
FaeI CATG 8 cut(s) 265, 358, 435, 506, 809, 841, 855, 1016
FaqI GGGAC 1 cut(s) 639
FatI CATG 8 cut(s) 261, 354, 431, 502, 805, 837, 851, 1012
FauI CCCGC 1 cut(s) 305
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 237
FokI GGATG 6 cut(s) 137, 371, 721, 774, 781, 912
FspBI CTAG 2 cut(s) 321, 990
GsaI CCCAGC 1 cut(s) 385
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 224
HapII CCGG 3 cut(s) 297, 344, 601
HgaI GACGC 1 cut(s) 1045
Hin1II CATG 8 cut(s) 265, 358, 435, 506, 809, 841, 855, 1016
HindIII AAGCTT 1 cut(s) 753
HinfI GANTC 4 cut(s) 230, 549, 590, 680
HpaII CCGG 3 cut(s) 297, 344, 601
HphI GGTGA 8 cut(s) 16, 46, 229, 794, 932, 1045, 1054, 1063
Hpy166II GTNNAC 5 cut(s) 142, 238, 318, 534, 923
Hpy188I TCNGA 6 cut(s) 229, 288, 304, 493, 702, 1077
Hpy188III TCNNGA 6 cut(s) 117, 154, 174, 277, 601, 1022
Hpy8I GTNNAC 5 cut(s) 142, 238, 318, 534, 923
HpyAV CCTTC 6 cut(s) 49, 113, 123, 670, 727, 910
HpyCH4III ACNGT 2 cut(s) 204, 979
HpyCH4IV ACGT 2 cut(s) 59, 245
HpyCH4V TGCA 5 cut(s) 255, 331, 445, 794, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 221, 752
HpyF3I CTNAG 2 cut(s) 76, 615
HpySE526I ACGT 2 cut(s) 59, 245
Hsp92II CATG 8 cut(s) 265, 358, 435, 506, 809, 841, 855, 1016
Kpn2I TCCGGA 1 cut(s) 600
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
LmnI GCTCC 3 cut(s) 493, 603, 703
LweI GCATC 3 cut(s) 147, 803, 890
MaeI CTAG 2 cut(s) 321, 990
MaeII ACGT 2 cut(s) 59, 245
MaeIII GTNAC 3 cut(s) 34, 217, 1033
MalI GATC 9 cut(s) 121, 172, 193, 260, 326, 405, 501, 645, 967
MbiI CCGCTC 1 cut(s) 800
MboI GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
MboII GAAGA 6 cut(s) 10, 13, 485, 556, 703, 742
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 2 cut(s) 191, 324
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 8 cut(s) 13, 82, 648, 655, 864, 882, 939, 1078
MlyI GAGTC 3 cut(s) 224, 558, 599
MmeI TCCRAC 3 cut(s) 252, 471, 725
MnlI CCTC 5 cut(s) 170, 235, 858, 1007, 1017
MroI TCCGGA 1 cut(s) 600
MroXI GAANNNNTTC 1 cut(s) 608
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 3 cut(s) 32, 155, 807
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 3 cut(s) 297, 344, 601
MspR9I CCNGG 3 cut(s) 108, 297, 706
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 108, 706
MvnI CGCG 2 cut(s) 175, 554
MwoI GCNNNNNNNGC 3 cut(s) 94, 221, 752
NciI CCSGG 1 cut(s) 297
NcoI CCATGG 3 cut(s) 431, 805, 851
NdeI CATATG 1 cut(s) 931
NdeII GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
NlaIII CATG 8 cut(s) 265, 358, 435, 506, 809, 841, 855, 1016
NlaIV GGNNCC 4 cut(s) 144, 495, 627, 738
NmuCI GTSAC 3 cut(s) 34, 217, 1033
NruI TCGCGA 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 807
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 1 cut(s) 680
PflFI GACNNNGTC 1 cut(s) 233
PleI GAGTC 3 cut(s) 224, 557, 598
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
PpsI GAGTC 3 cut(s) 224, 557, 598
Ppu21I YACGTR 2 cut(s) 60, 246
PpuMI RGGWCCY 3 cut(s) 100, 110, 736
Psp5II RGGWCCY 3 cut(s) 100, 110, 736
Psp6I CCWGG 2 cut(s) 106, 704
PspCI CACGTG 1 cut(s) 246
PspEI GGTNACC 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 381
PspGI CCWGG 2 cut(s) 106, 704
PspN4I GGNNCC 4 cut(s) 144, 495, 627, 738
PspPI GGNCC 5 cut(s) 100, 110, 142, 626, 736
PspPPI RGGWCCY 3 cut(s) 100, 110, 736
PsrI GAACNNNNNNTAC 2 cut(s) 257, 289
PsuI RGATCY 2 cut(s) 191, 324
PsyI GACNNNGTC 1 cut(s) 233
RruI TCGCGA 1 cut(s) 175
RsaI GTAC 4 cut(s) 353, 587, 827, 976
RsaNI GTAC 4 cut(s) 352, 586, 826, 975
RseI CAYNNNNRTG 3 cut(s) 32, 155, 807
SaqAI TTAA 1 cut(s) 687
Sau3AI GATC 9 cut(s) 119, 170, 191, 258, 324, 403, 499, 643, 965
Sau96I GGNCC 5 cut(s) 100, 110, 142, 626, 736
SchI GAGTC 3 cut(s) 224, 558, 599
ScrFI CCNGG 3 cut(s) 108, 297, 706
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 3 cut(s) 147, 803, 890
SinI GGWCC 5 cut(s) 100, 110, 142, 626, 736
SmiMI CAYNNNNRTG 3 cut(s) 32, 155, 807
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 8 cut(s) 13, 82, 648, 655, 864, 882, 939, 1078
SsiI CCGC 4 cut(s) 215, 312, 798, 986
SspMI CTAG 2 cut(s) 321, 990
StyD4I CCNGG 3 cut(s) 106, 295, 704
StyI CCWWGG 3 cut(s) 431, 805, 851
TaaI ACNGT 2 cut(s) 204, 979
TaiI ACGT 2 cut(s) 62, 248
TaqI TCGA 2 cut(s) 116, 131
TasI AATT 8 cut(s) 13, 82, 648, 655, 864, 882, 939, 1078
TatI WGTACW 2 cut(s) 351, 825
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 3 cut(s) 257, 541, 600
TseFI GTSAC 3 cut(s) 34, 217, 1033
Tsp45I GTSAC 3 cut(s) 34, 217, 1033
TspDTI ATGAA 8 cut(s) 375, 725, 743, 826, 868, 875, 929, 1071
TspGWI ACGGA 2 cut(s) 34, 223
TspRI CASTG 3 cut(s) 257, 541, 600
Tth111I GACNNNGTC 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 686
VpaK11BI GGWCC 5 cut(s) 100, 110, 142, 626, 736
XapI RAATTY 2 cut(s) 13, 655
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 2 cut(s) 321, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.