pycom15g12770

Protein of unknown function (DUF2996)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006674
Physical Location & Seq
Forward (+)
8744736 .. 8745994
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGATAGGCCAAGAGAAGAGAAGATGGTTTTATTTCAGTGGCGAATTAAGCTCCAAATTGATCACCAAGCAAGCAAGCATGGCTTCGGCTATTCTTTCTCTGCCCAAACCATCTCCTCCGCAGGTTCAGCTTCATGCTTTTCAATGTCGACGACGACGACAGCCAAATGGAAAGCATTTGGCAGAATTCTCAGCTCTCCCCAAGTCATCAGCCCGCCTTCCTTTTCGAAGGAAGACCTCAAACCATGAAATGATGAAAAGTAAAGTGGTAGCATCTCAAAGAAAAAGAAACATTGCCTGGTCTGCTTTGCAAGAATCATCCACTTCCACCGCTGATGCTGCTGATACAAGTACGAAGGAGGTTAGAACAGCTGATCAAAAAGCAGCAGCTCCGGCGAAGCCTAAAGTTGCAGCGAAAGCTCCAGTGAAGGCGTTGCCTCAAATGATGGAGGAGGACGTTATCCCGCCATTGAAAGCAATACTCCAAACTCAAGATGAACTCTCTGATATTGAGCTTTGTTTTCAAGACAACAGGCTGGAAGGTTCCTTTATAAAGAAGGGCAATACCTACTCGTTTTGGGTCTTCTTTCCCAGTGGAGTCCTCACAGGTCCAAAGGGGTTTTCGCTGTCTTCGTATGGCTCAGAAGCGAGCACTGTTGAGCCTTTTCTCGTTGATGAGAAGAAAATCACAGCAAAACATATTGTCTTTTGGGTTGAAAAGCGTTTAGCCGCTCAAGGAATCATTCCTGTCTGGAAATTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2996

Protein Analysis

255

Amino Acids

28.34

Weight (kDa)

9.88

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2996 PF11210 146 - 245 1.2e-13 Protein of unknown function (DUF2996)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 551
Acc36I ACCTGC 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 731
AccI GTMKAC 1 cut(s) 148
AciI CCGC 5 cut(s) 119, 214, 330, 464, 729
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 352
AgsI TTSAA 4 cut(s) 143, 472, 524, 716
AjnI CCWGG 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 604, 636
AluBI AGCT 7 cut(s) 51, 130, 194, 371, 389, 419, 514
AluI AGCT 7 cut(s) 51, 130, 194, 371, 389, 419, 514
Alw21I GWGCWC 1 cut(s) 653
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 4 cut(s) 338, 383, 386, 410
ApoI RAATTY 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 608
AsuHPI GGTGA 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 608
BbsI GAAGAC 3 cut(s) 239, 574, 621
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 4 cut(s) 325, 395, 398, 422
BccI CCATC 3 cut(s) 18, 118, 439
BciT130I CCWGG 1 cut(s) 298
BclI TGATCA 2 cut(s) 60, 373
BfaI CTAG 1 cut(s) 763
BfuAI ACCTGC 1 cut(s) 112
BisI GCNGC 5 cut(s) 339, 384, 387, 411, 729
BlsI GCNGC 5 cut(s) 340, 385, 388, 412, 730
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 608
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 585
BmsI GCATC 2 cut(s) 281, 325
BmuI ACTGGG 1 cut(s) 585
BpiI GAAGAC 3 cut(s) 239, 574, 621
BpmI CTGGAG 1 cut(s) 405
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 474, 717
Bse1I ACTGG 2 cut(s) 422, 591
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 298
BseGI GGATG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 2 cut(s) 204, 654
BseNI ACTGG 2 cut(s) 422, 591
BseRI GAGGAG 2 cut(s) 105, 464
BseXI GCAGC 4 cut(s) 325, 395, 398, 422
BshFI GGCC 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 653
BsiSI CCGG 1 cut(s) 392
BsnI GGCC 1 cut(s) 9
Bsp119I TTCGAA 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 2 cut(s) 60, 373
BspACI CCGC 5 cut(s) 119, 214, 330, 464, 729
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 2 cut(s) 203, 653
BspLI GGNNCC 1 cut(s) 544
BspMI ACCTGC 1 cut(s) 112
BspT104I TTCGAA 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 2 cut(s) 422, 591
BssMI GATC 2 cut(s) 60, 373
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 1 cut(s) 655
Bst6I CTCTTC 1 cut(s) 11
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 72, 76, 214, 649
BstDEI CTNAG 2 cut(s) 190, 640
BstF5I GGATG 1 cut(s) 317
BstKTI GATC 2 cut(s) 63, 376
BstMBI GATC 2 cut(s) 60, 373
BstMWI GCNNNNNNNGC 7 cut(s) 48, 80, 127, 302, 338, 392, 416
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 325, 395, 398, 422
BstV2I GAAGAC 3 cut(s) 239, 574, 621
BsuRI GGCC 1 cut(s) 9
BtsCI GGATG 1 cut(s) 317
BtsIMutI CAGTG 4 cut(s) 43, 429, 598, 651
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 4 cut(s) 72, 76, 214, 649
Cfr13I GGNCC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 351
CviAII CATG 3 cut(s) 79, 134, 245
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 2 cut(s) 190, 640
DpnI GATC 2 cut(s) 62, 375
DpnII GATC 2 cut(s) 60, 373
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 296
FaeI CATG 3 cut(s) 82, 137, 248
FaiI YATR 6 cut(s) 80, 135, 246, 551, 636, 699
FalI AAGNNNNNCTT 2 cut(s) 67, 99
FatI CATG 3 cut(s) 78, 133, 244
FauI CCCGC 2 cut(s) 221, 471
FbaI TGATCA 2 cut(s) 60, 373
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 5 cut(s) 339, 384, 387, 411, 729
FokI GGATG 1 cut(s) 304
Fsp4HI GCNGC 5 cut(s) 339, 384, 387, 411, 729
FspBI CTAG 1 cut(s) 763
GluI GCNGC 5 cut(s) 339, 384, 387, 411, 729
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 392
Hin1II CATG 3 cut(s) 82, 137, 248
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HinfI GANTC 3 cut(s) 314, 597, 738
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 2 cut(s) 505, 643
Hpy188III TCNNGA 3 cut(s) 491, 524, 751
Hpy8I GTNNAC 1 cut(s) 149
Hpy99I CGWCG 3 cut(s) 153, 156, 159
HpyAV CCTTC 6 cut(s) 222, 227, 349, 421, 533, 550
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 310, 410
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 80, 127, 302, 338, 392, 416
HpyF3I CTNAG 2 cut(s) 190, 640
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 3 cut(s) 82, 137, 248
Ksp22I TGATCA 2 cut(s) 60, 373
Kzo9I GATC 2 cut(s) 60, 373
LmnI GCTCC 3 cut(s) 56, 394, 424
Lsp1109I GCAGC 4 cut(s) 325, 395, 398, 422
LweI GCATC 2 cut(s) 281, 325
MaeI CTAG 1 cut(s) 763
MaeII ACGT 1 cut(s) 456
MalI GATC 2 cut(s) 62, 375
MbiI CCGCTC 1 cut(s) 731
MboI GATC 2 cut(s) 60, 373
MboII GAAGA 6 cut(s) 28, 33, 244, 574, 621, 691
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 4 cut(s) 44, 56, 185, 755
MlyI GAGTC 1 cut(s) 606
MnlI CCTC 7 cut(s) 126, 247, 352, 442, 445, 447, 611
MseI TTAA 1 cut(s) 47
MspA1I CMGCKG 2 cut(s) 332, 371
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 298
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 7 cut(s) 48, 80, 127, 302, 338, 392, 416
NdeII GATC 2 cut(s) 60, 373
NlaIII CATG 3 cut(s) 82, 137, 248
NlaIV GGNNCC 1 cut(s) 544
NspV TTCGAA 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 154, 629
PfeI GAWTC 2 cut(s) 314, 738
PkrI GCNGC 5 cut(s) 340, 385, 388, 412, 730
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
PsiI TTATAA 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SalI GTCGAC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 5 cut(s) 339, 384, 387, 411, 729
Sau3AI GATC 2 cut(s) 60, 373
Sau96I GGNCC 1 cut(s) 608
SchI GAGTC 1 cut(s) 606
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 2 cut(s) 281, 325
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 1 cut(s) 608
SmlI CTYRAG 2 cut(s) 489, 732
SmoI CTYRAG 2 cut(s) 489, 732
Sse9I AATT 4 cut(s) 44, 56, 185, 755
SsiI CCGC 5 cut(s) 119, 214, 330, 464, 729
SspMI CTAG 1 cut(s) 763
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 148, 226
TasI AATT 4 cut(s) 44, 56, 185, 755
TauI GCSGC 1 cut(s) 731
TfiI GAWTC 2 cut(s) 314, 738
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 4 cut(s) 43, 429, 598, 658
TseI GCWGC 4 cut(s) 338, 383, 386, 410
TspDTI ATGAA 4 cut(s) 122, 261, 269, 510
TspRI CASTG 4 cut(s) 43, 429, 598, 658
VpaK11BI GGWCC 1 cut(s) 608
XapI RAATTY 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.