pycom15g13360

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005062
Physical Location & Seq
Forward (+)
9128404 .. 9130082
1679 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 312 bp
ATGGCCATGTATATCCGTGTTAAGCGTAGCAAGACAACTTACTTTATCCAGTGTGAACCAACTGAGACAGGTTTAGATATTAAGCAGAAATTGCATGATCTTACTGATCAACCGGTCAATGATCAGCGCTTGATCTTAGTCAGTACCGGGGAAGTATTGGAGGATTCAAAGACGTTGGCAGATCAGAAGGTTGAAAATGATGCCGTTGTTGCATTGGCCTTCAGAAAAGATGACAATGAGTTTGAAGAGGTCAACATTGTACGACCAGACGACTTCTATCTGTCTCGTGATGCAGATGCTGGCAATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04066 ko04120 ko05200 ko05211 map04066 map04120 map05200 map05211
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

104

Amino Acids

11.85

Weight (kDa)

4.52

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 5 - 76 8.5e-13 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 205
AfaI GTAC 2 cut(s) 145, 261
AfeI AGCGCT 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 3 cut(s) 168, 194, 245
Alw26I GTCTC 2 cut(s) 59, 288
AlwNI CAGNNNCTG 1 cut(s) 299
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 2 cut(s) 3, 216
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 148
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 285
BceAI ACGGC 1 cut(s) 188
BclI TGATCA 2 cut(s) 106, 121
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 59, 288
BfoI RGCGCY 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 3 cut(s) 190, 280, 286
BpuMI CCSGG 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 112
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 49
BshFI GGCC 2 cut(s) 5, 218
BshTI ACCGGT 1 cut(s) 112
BsiSI CCGG 2 cut(s) 113, 147
BsmAI GTCTC 2 cut(s) 59, 288
BsnI GGCC 2 cut(s) 5, 218
Bsp143I GATC 5 cut(s) 97, 106, 121, 132, 181
BspANI GGCC 2 cut(s) 5, 218
BspCNI CTCAG 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 5 cut(s) 97, 106, 121, 132, 181
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst6I CTCTTC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 2 cut(s) 63, 136
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 100, 109, 124, 135, 184
BstMAI GTCTC 2 cut(s) 59, 288
BstMBI GATC 5 cut(s) 97, 106, 121, 132, 181
BstMWI GCNNNNNNNGC 2 cut(s) 91, 209
BstSCI CCNGG 1 cut(s) 146
BsuRI GGCC 2 cut(s) 5, 218
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 112
Csp6I GTAC 2 cut(s) 144, 260
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 2 cut(s) 7, 95
CviJI RGCY 2 cut(s) 5, 218
CviKI_1 RGCY 2 cut(s) 5, 218
CviQI GTAC 2 cut(s) 144, 260
DdeI CTNAG 2 cut(s) 63, 136
DpnI GATC 5 cut(s) 99, 108, 123, 134, 183
DpnII GATC 5 cut(s) 97, 106, 121, 132, 181
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco47III AGCGCT 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 205
FaeI CATG 2 cut(s) 10, 98
FaiI YATR 3 cut(s) 8, 12, 96
FatI CATG 2 cut(s) 6, 94
FbaI TGATCA 2 cut(s) 106, 121
GlaI GCGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 2 cut(s) 5, 218
HapII CCGG 2 cut(s) 113, 147
HhaI GCGC 1 cut(s) 129
Hin1II CATG 2 cut(s) 10, 98
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 164
HpaII CCGG 2 cut(s) 113, 147
Hpy166II GTNNAC 2 cut(s) 56, 253
Hpy188I TCNGA 2 cut(s) 186, 224
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 2 cut(s) 56, 253
HpyAV CCTTC 2 cut(s) 181, 229
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 3 cut(s) 94, 212, 293
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 209
HpyF3I CTNAG 2 cut(s) 63, 136
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 2 cut(s) 10, 98
HspAI GCGC 1 cut(s) 127
Ksp22I TGATCA 2 cut(s) 106, 121
Kzo9I GATC 5 cut(s) 97, 106, 121, 132, 181
LpnPI CCDG 6 cut(s) 54, 62, 126, 160, 279, 285
LweI GCATC 3 cut(s) 190, 280, 286
MaeII ACGT 1 cut(s) 173
MalI GATC 5 cut(s) 99, 108, 123, 134, 183
MboI GATC 5 cut(s) 97, 106, 121, 132, 181
MboII GAAGA 1 cut(s) 257
MfeI CAATTG 1 cut(s) 304
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 89, 304
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 154, 241
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 21, 81
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 113, 147
MspR9I CCNGG 1 cut(s) 148
MunI CAATTG 1 cut(s) 304
MwoI GCNNNNNNNGC 2 cut(s) 91, 209
NciI CCSGG 1 cut(s) 148
NdeII GATC 5 cut(s) 97, 106, 121, 132, 181
NlaIII CATG 2 cut(s) 10, 98
PfeI GAWTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 299
RsaI GTAC 2 cut(s) 145, 261
RsaNI GTAC 2 cut(s) 144, 260
SaqAI TTAA 2 cut(s) 21, 81
Sau3AI GATC 5 cut(s) 97, 106, 121, 132, 181
ScrFI CCNGG 1 cut(s) 148
SetI ASST 4 cut(s) 73, 176, 192, 252
SfaNI GCATC 3 cut(s) 190, 280, 286
Sse9I AATT 2 cut(s) 89, 304
StyD4I CCNGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 176
TasI AATT 2 cut(s) 89, 304
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 21, 81
Tru9I TTAA 2 cut(s) 21, 81
TscAI CASTG 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 5
TspRI CASTG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.