pycom15g13860

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014164
Physical Location & Seq
Forward (+)
9463989 .. 9465134
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGCATCCCAAACTTCAAAATTTCCAAATCTTCCTGCAATCTCCCGATCTCCAAATCCCAACCCAAACCCTAACCCTATCCCCAATTCAGACCCCAACTCTCCGCCACCTCAAGCTCTCAATTTTCTCCCGTGTCCTACCGTCGCTTTACTTCACCCTCAATGGTAAGCCCCTAAACGACTCCACTCCGCTTTTCGATTCCCAAATTACCCCTCTTTCTACTCTGATTTTACGGATTAGAGCTCTTGGAGGTGGCGGCGATGGCGGCGCGACGGGAGCGGAGTCTCGCGACTGCTATCTCAAGATGTACGCGGAGAAGAAGCCCGATAAGGTTGACCCGAACGAGCAGAGGCTTTCGAAGTGGCTCAATTGTGCGCTGTCGAACGAACCATTGAAGGAGCCGTGCGTAATGGATTTTCTGGGGAACGTGTTTAACAAGGAGGCTCTTGTGGAGGCGTTGTTGGGGAAGAAGGTGCCCAAGGCGTTTGGGCATATAAAGGGGTTGAAGGACATGATCAGTATTCATCTTACCCCAATTACTGGGGCCGAATTCAATCGCCAATCCGTTGCTGGGCCGCGGTTTCAGTGCCCCATTACAGGGGTCGAGTTCAACGGTAAGTGTAAGTTTGTGGGATTAAGAACTTGTGGGCATGTGTTGAGTTCAAAGGCTTTGAAGGAGATAAAGTCGTCGACTTGCCTTGTTTGCCATGTGGGATTTTCAGAGGCGGATAAGATTGTGATTAATGGGAATGAAGAGGAAGTTGTGGAGTTGAGGGAGAGGATGGAGGCTGAGAAGGCGAAAGGGAGAGTGAAGAAGGTGAAGAAGACGAAAAATGGGAATGCTGGTGTGAATGGCGAAGAGGAGGGTGTGGGTTTGGAGGCCTCACGGTTGAGTGGTACCAAGCATGGTATTGATGCTAGGGCTGTGGAGAAGGTTTCAGCAAAGGTAGAGGTAAATGGGAAGGTTGTGAATGTTGGAGCTGATGTGAAGGGTGCAAGTAATGGTGGGGCAAAGAGGTTCAAGGCGGTGGATATAGCCCCTGCTAATGCTACTAAGGAAGTGTATGCGTCCATCTTCACGTCGTCAAGGAAGAAGGATTTCAAGGAAACATTTTCTTGCAGATCTCTCCCACTTGGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

382

Amino Acids

41.42

Weight (kDa)

9.44

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 84 - 365 3e-75 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 896
AccB1I GGYRCC 2 cut(s) 472, 896
AccB7I CCANNNNNTGG 1 cut(s) 539
AccBSI CCGCTC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 689
AccII CGCG 4 cut(s) 269, 288, 311, 577
AcsI RAATTY 2 cut(s) 19, 548
AfaI GTAC 2 cut(s) 308, 898
AfiI CCNNNNNNNGG 4 cut(s) 539, 570, 596, 597
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 9 cut(s) 17, 394, 505, 553, 610, 663, 673, 1021, 1102
AjiI CACGTC 1 cut(s) 1080
AluBI AGCT 3 cut(s) 115, 242, 980
AluI AGCT 3 cut(s) 115, 242, 980
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 288
AoxI GGCC 3 cut(s) 543, 572, 879
ApoI RAATTY 2 cut(s) 19, 548
AseI ATTAAT 1 cut(s) 741
Asp700I GAANNNNTTC 1 cut(s) 1097
Asp718I GGTACC 1 cut(s) 896
AspLEI GCGC 2 cut(s) 269, 376
AspS9I GGNCC 2 cut(s) 543, 572
AsuHPI GGTGA 2 cut(s) 145, 829
AsuII TTCGAA 1 cut(s) 356
BaeGI GKGCMC 2 cut(s) 477, 590
BanI GGYRCC 2 cut(s) 472, 896
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 1 cut(s) 244
BccI CCATC 3 cut(s) 254, 775, 1079
BceAI ACGGC 1 cut(s) 385
BclI TGATCA 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 918
BglII AGATCT 1 cut(s) 1121
BisI GCNGC 3 cut(s) 256, 265, 575
BlsI GCNGC 3 cut(s) 257, 266, 576
BmgBI CACGTC 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 543, 572
BmiI GGNNCC 4 cut(s) 399, 474, 544, 898
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 13, 904
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 830
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 95, 284
BsaJI CCNNGG 2 cut(s) 477, 575
BsaXI ACNNNNNCTCC 6 cut(s) 443, 473, 668, 698, 969, 999
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 570, 596, 597
Bse1I ACTGG 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 477, 575
BseGI GGATG 2 cut(s) 4, 786
BseLI CCNNNNNNNGG 4 cut(s) 539, 570, 596, 597
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 2 cut(s) 477, 590
BseYI CCCAGC 1 cut(s) 569
Bsh1236I CGCG 4 cut(s) 269, 288, 311, 577
BshFI GGCC 3 cut(s) 545, 574, 881
BshNI GGYRCC 2 cut(s) 472, 896
BsiHKAI GWGCWC 1 cut(s) 244
BslI CCNNNNNNNGG 4 cut(s) 539, 570, 596, 597
BsmAI GTCTC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 3 cut(s) 545, 574, 881
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 3 cut(s) 244, 477, 590
Bsp143I GATC 3 cut(s) 46, 513, 1121
Bsp68I TCGCGA 1 cut(s) 288
BspANI GGCC 3 cut(s) 545, 574, 881
BspCNI CTCAG 1 cut(s) 781
BspFNI CGCG 4 cut(s) 269, 288, 311, 577
BspLI GGNNCC 4 cut(s) 399, 474, 544, 898
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 472, 896
BsrBI CCGCTC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 477, 575
BssMI GATC 3 cut(s) 46, 513, 1121
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 141, 614, 888
Bst6I CTCTTC 2 cut(s) 747, 852
BstBI TTCGAA 1 cut(s) 356
BstDEI CTNAG 2 cut(s) 789, 1053
BstDSI CCRYGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 4, 786
BstFNI CGCG 4 cut(s) 269, 288, 311, 577
BstHHI GCGC 2 cut(s) 269, 376
BstKTI GATC 3 cut(s) 49, 516, 1124
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 3 cut(s) 46, 513, 1121
BstMWI GCNNNNNNNGC 5 cut(s) 261, 264, 275, 702, 794
BstNSI RCATGY 1 cut(s) 653
BstSLI GKGCMC 2 cut(s) 477, 590
BstUI CGCG 4 cut(s) 269, 288, 311, 577
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BsuRI GGCC 3 cut(s) 545, 574, 881
BtgI CCRYGG 1 cut(s) 575
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 1080
BtsCI GGATG 2 cut(s) 4, 786
BtsIMutI CAGTG 1 cut(s) 590
BtuMI TCGCGA 1 cut(s) 288
CfoI GCGC 2 cut(s) 269, 376
Cfr13I GGNCC 2 cut(s) 543, 572
Cfr42I CCGCGG 1 cut(s) 578
CseI GACGC 1 cut(s) 1056
Csp6I GTAC 2 cut(s) 307, 897
CviAII CATG 4 cut(s) 511, 650, 707, 905
CviQI GTAC 2 cut(s) 307, 897
DdeI CTNAG 2 cut(s) 789, 1053
DpnI GATC 3 cut(s) 48, 515, 1123
DpnII GATC 3 cut(s) 46, 513, 1121
Eam1104I CTCTTC 2 cut(s) 747, 852
EarI CTCTTC 2 cut(s) 747, 852
EciI GGCGGA 2 cut(s) 92, 740
Ecl136II GAGCTC 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 477
Eco147I AGGCCT 1 cut(s) 881
Eco24I GRGCYC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 548
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 4 cut(s) 514, 653, 710, 908
FaiI YATR 8 cut(s) 492, 494, 512, 651, 708, 906, 1034, 1065
FatI CATG 4 cut(s) 510, 649, 706, 904
FbaI TGATCA 1 cut(s) 513
FblI GTMKAC 1 cut(s) 689
Fnu4HI GCNGC 3 cut(s) 256, 265, 575
FokI GGATG 1 cut(s) 793
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 3 cut(s) 256, 265, 575
FspBI CTAG 1 cut(s) 918
GlaI GCGC 2 cut(s) 268, 375
GluI GCNGC 3 cut(s) 256, 265, 575
GsaI CCCAGC 1 cut(s) 573
HaeIII GGCC 3 cut(s) 545, 574, 881
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 2 cut(s) 269, 376
Hin1II CATG 4 cut(s) 514, 653, 710, 908
Hin6I GCGC 2 cut(s) 267, 374
HinP1I GCGC 2 cut(s) 267, 374
HincII GTYRAC 2 cut(s) 334, 690
HindII GTYRAC 2 cut(s) 334, 690
HinfI GANTC 3 cut(s) 179, 197, 281
HphI GGTGA 2 cut(s) 145, 829
Hpy166II GTNNAC 2 cut(s) 334, 690
Hpy188I TCNGA 3 cut(s) 90, 225, 721
Hpy188III TCNNGA 3 cut(s) 44, 287, 301
Hpy8I GTNNAC 2 cut(s) 334, 690
Hpy99I CGWCG 4 cut(s) 145, 274, 691, 1084
HpyCH4III ACNGT 3 cut(s) 141, 614, 888
HpyCH4IV ACGT 2 cut(s) 426, 1079
HpyCH4V TGCA 4 cut(s) 4, 37, 995, 1119
HpyF10VI GCNNNNNNNGC 5 cut(s) 261, 264, 275, 702, 794
HpyF3I CTNAG 2 cut(s) 789, 1053
HpySE526I ACGT 2 cut(s) 426, 1079
Hsp92II CATG 4 cut(s) 514, 653, 710, 908
HspAI GCGC 2 cut(s) 267, 374
KpnI GGTACC 1 cut(s) 900
Ksp22I TGATCA 1 cut(s) 513
KspI CCGCGG 1 cut(s) 578
Kzo9I GATC 3 cut(s) 46, 513, 1121
LmnI GCTCC 3 cut(s) 275, 397, 977
LpnPI CCDG 7 cut(s) 47, 404, 525, 555, 582, 828, 1053
LweI GCATC 2 cut(s) 13, 904
MaeI CTAG 1 cut(s) 918
MaeII ACGT 2 cut(s) 426, 1079
MalI GATC 3 cut(s) 48, 515, 1123
MbiI CCGCTC 1 cut(s) 278
MboI GATC 3 cut(s) 46, 513, 1121
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 3 cut(s) 244, 477, 590
MluCI AATT 7 cut(s) 19, 84, 120, 204, 367, 534, 548
MlyI GAGTC 2 cut(s) 173, 290
MmeI TCCRAC 1 cut(s) 955
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1097
MseI TTAA 3 cut(s) 432, 635, 741
MspA1I CMGCKG 1 cut(s) 577
MunI CAATTG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 4 cut(s) 269, 288, 311, 577
MwoI GCNNNNNNNGC 5 cut(s) 261, 264, 275, 702, 794
NdeII GATC 3 cut(s) 46, 513, 1121
NlaIII CATG 4 cut(s) 514, 653, 710, 908
NlaIV GGNNCC 4 cut(s) 399, 474, 544, 898
NruI TCGCGA 1 cut(s) 288
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 356
PceI AGGCCT 1 cut(s) 881
PctI GAATGC 1 cut(s) 844
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 3 cut(s) 257, 266, 576
PleI GAGTC 2 cut(s) 173, 289
PpsI GAGTC 2 cut(s) 173, 289
PshBI ATTAAT 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 569
PspN4I GGNNCC 4 cut(s) 399, 474, 544, 898
PspPI GGNCC 2 cut(s) 543, 572
PsuI RGATCY 1 cut(s) 1121
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 2 cut(s) 308, 898
RsaNI GTAC 2 cut(s) 307, 897
SacI GAGCTC 1 cut(s) 244
SacII CCGCGG 1 cut(s) 578
SalI GTCGAC 1 cut(s) 688
SaqAI TTAA 3 cut(s) 432, 635, 741
SatI GCNGC 3 cut(s) 256, 265, 575
Sau3AI GATC 3 cut(s) 46, 513, 1121
Sau96I GGNCC 2 cut(s) 543, 572
SchI GAGTC 2 cut(s) 173, 290
SduI GDGCHC 3 cut(s) 244, 477, 590
SfaNI GCATC 2 cut(s) 13, 904
Sfr303I CCGCGG 1 cut(s) 578
SfuI TTCGAA 1 cut(s) 356
SgrBI CCGCGG 1 cut(s) 578
SmlI CTYRAG 2 cut(s) 110, 299
SmoI CTYRAG 2 cut(s) 110, 299
Sse9I AATT 7 cut(s) 19, 84, 120, 204, 367, 534, 548
SseBI AGGCCT 1 cut(s) 881
SspMI CTAG 1 cut(s) 918
SstI GAGCTC 1 cut(s) 244
StuI AGGCCT 1 cut(s) 881
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 141, 614, 888
TaiI ACGT 2 cut(s) 429, 1082
TaqI TCGA 5 cut(s) 195, 356, 380, 603, 689
TasI AATT 7 cut(s) 19, 84, 120, 204, 367, 534, 548
TauI GCSGC 3 cut(s) 258, 267, 577
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 3 cut(s) 432, 635, 741
Tru9I TTAA 3 cut(s) 432, 635, 741
TscAI CASTG 1 cut(s) 590
TspDTI ATGAA 2 cut(s) 512, 765
TspGWI ACGGA 2 cut(s) 247, 553
TspRI CASTG 1 cut(s) 590
Van91I CCANNNNNTGG 1 cut(s) 539
VspI ATTAAT 1 cut(s) 741
XapI RAATTY 2 cut(s) 19, 548
XceI RCATGY 1 cut(s) 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 1 cut(s) 918
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.