pycom15g15490

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014535
Physical Location & Seq
Forward (+)
10781427 .. 10783247
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGCAGGGGGGCTATTTTTATCTGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGGGGTCGTTAGTTTCCGCCGCCATTGCTTGTTCAACCGAACCTGCAAATCTAACGACTTTTGATGTCAGAGACTTCGGAGCTATCAGTGCCGGAGGCAACATTGATAATTCCGAAGCATTCTTGCTGGCTTGGAATGCAACATGCAACTCAGAATCTGAAATTTCTACCTTGATCGTTCCACGACGGAAATTTCTGGTTAATCCGGTCGTCTTCGGCGGTCCATGCAAGCCAAAAATCATAAATTTTTTGATACTAGGGAGACTTTTGGCACCAAACTCACCTGAAGCATGGAAGGAGCTTGACCAAAGTCAATGGCTAGCTTTCAAACGAGTCAGCGGGCTCAAGGTGGTTGGCCCTGGCAGAATCAATGGAAGAGGCAAAGGCTGGTGGGATCAGTCCTGCAGAGACCACCATGGCCTGGTACATTTTCAAATGCAGCCGCCCGGATGCACTGTATTGGCCCCAACTGCAGTGAAGTTTGTGTCGTGTAACAACAGCAGCATTAGTGAGGTTCATTTCGTAAATAGCTCTCAAGTCCATGTCTTAATAGAAGGGTGTGAAGGAATTCACATAGAGAATGTGCTGATCGAAGCTCCGGAAAGAAGCCCCAACACTGACGGAATTCACATTTCTGCCTCTCACTACGTGTTTGTCACTAATGCAATTATAGCAACGGTTGACATTGATACGAAATTAATGATTTCATTAACAGGCGATGATTGTGTTTCCATTGGAGACCATACATCCAATATAGTCGTCTCGTACGTTAAATGCGGCCCCGGCTACGGAATCAGCATTGGCAGCTTAGGGAGGAGTGGGAATTTTGTTCAGGTGGAGAACATTCATGTAACTCAGGTCTACCTCCAAGGAACCACCAACGGAGCTCGAATCAAGACATGGCAGGTACCGAATGAAAACCCGAAATTTCGGAAACAAATTCGAGAGACATTCTCATTCAATCTTCAGTGTTTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

346

Amino Acids

37.68

Weight (kDa)

6.55

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 71 - 321 1.6e-50 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80140
fragaria_vesca FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310
prunus_persica Prupe.7G242600_v2.0.a1
pyrus_communis pycom15g15490
rosa_chinensis RchiOBHm_Chr2g0088471
rosa_laevigata RLG00000015957
rosa_roxburghii Rroxscaffold_2G00152660
rosa_rugosa Rorug01G0484300
rosa_samantha Rh2AG039400 Rh2BG038200 Rh2CG039500 Rh2DG038900
rosa_wichuraiana Rw2G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 109, 952
Acc65I GGTACC 1 cut(s) 964
AccB1I GGYRCC 2 cut(s) 328, 964
AccB7I CCANNNNNTGG 1 cut(s) 478
AccI GTMKAC 1 cut(s) 918
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 6 cut(s) 75, 78, 276, 396, 500, 834
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 8 cut(s) 219, 248, 301, 624, 681, 880, 983, 996
AcuI CTGAAG 2 cut(s) 363, 1007
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 3 cut(s) 483, 824, 966
AfiI CCNNNNNNNGG 2 cut(s) 478, 845
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 4 cut(s) 93, 385, 491, 1018
AjnI CCWGG 2 cut(s) 415, 477
AluBI AGCT 7 cut(s) 140, 358, 380, 588, 653, 864, 944
AluI AGCT 7 cut(s) 140, 358, 380, 588, 653, 864, 944
Alw21I GWGCWC 1 cut(s) 946
Alw26I GTCTC 6 cut(s) 123, 313, 459, 789, 823, 998
AlwI GGATC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 4 cut(s) 412, 475, 519, 835
ApeKI GCWGC 3 cut(s) 496, 558, 861
ApoI RAATTY 8 cut(s) 219, 248, 301, 624, 681, 880, 983, 996
AseI ATTAAT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 624
Asp718I GGTACC 1 cut(s) 964
AspS9I GGNCC 4 cut(s) 278, 413, 520, 836
AsuC2I CCSGG 2 cut(s) 504, 840
AsuHPI GGTGA 1 cut(s) 330
AsuNHI GCTAGC 1 cut(s) 376
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 2 cut(s) 328, 964
BanII GRGCYC 2 cut(s) 402, 946
BbsI GAAGAC 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 946
BbvI GCAGC 3 cut(s) 508, 570, 873
BciT130I CCWGG 2 cut(s) 417, 479
BcnI CCSGG 2 cut(s) 504, 840
BcoDI GTCTC 6 cut(s) 123, 313, 459, 789, 823, 998
BfaI CTAG 2 cut(s) 314, 377
BfmI CTRYAG 2 cut(s) 460, 528
BfuAI ACCTGC 2 cut(s) 109, 952
BisI GCNGC 6 cut(s) 78, 497, 500, 559, 835, 862
BlsI GCNGC 6 cut(s) 79, 498, 501, 560, 836, 863
Bme1390I CCNGG 4 cut(s) 417, 479, 504, 840
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 278, 413, 520, 836
BmiI GGNNCC 5 cut(s) 330, 522, 838, 931, 966
BmrFI CCNGG 4 cut(s) 417, 479, 504, 840
BmsI GCATC 1 cut(s) 497
BmtI GCTAGC 1 cut(s) 380
BoxI GACNNNNGTC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 262
BplI GAGNNNNNCTC 2 cut(s) 995, 1027
Bpu10I CCTNAGC 1 cut(s) 865
BpuEI CTTGAG 2 cut(s) 386, 576
BpuMI CCSGG 2 cut(s) 504, 840
BsaAI YACGTR 1 cut(s) 706
BsaI GGTCTC 2 cut(s) 459, 789
BsaJI CCNNGG 4 cut(s) 415, 472, 838, 925
BsaWI WCCGGW 2 cut(s) 262, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 845
Bse3DI GCAATG 1 cut(s) 81
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 2 cut(s) 417, 479
BseDI CCNNGG 4 cut(s) 415, 472, 838, 925
BseGI GGATG 2 cut(s) 512, 803
BseLI CCNNNNNNNGG 2 cut(s) 478, 845
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 2 cut(s) 222, 926
BseRI GAGGAG 1 cut(s) 886
BseXI GCAGC 3 cut(s) 508, 570, 873
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 4 cut(s) 414, 477, 521, 837
BshNI GGYRCC 2 cut(s) 328, 964
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 946
BsiSI CCGG 5 cut(s) 150, 263, 504, 656, 840
BsiWI CGTACG 1 cut(s) 822
BslI CCNNNNNNNGG 2 cut(s) 478, 845
BsmAI GTCTC 6 cut(s) 123, 313, 459, 789, 823, 998
BsmBI CGTCTC 1 cut(s) 823
BsmI GAATGC 2 cut(s) 176, 199
BsnI GGCC 4 cut(s) 414, 477, 521, 837
Bso31I GGTCTC 2 cut(s) 459, 789
Bsp1286I GDGCHC 2 cut(s) 402, 946
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 3 cut(s) 231, 451, 645
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 6 cut(s) 75, 78, 276, 396, 500, 834
BspANI GGCC 4 cut(s) 414, 477, 521, 837
BspCNI CTCAG 2 cut(s) 221, 925
BspEI TCCGGA 1 cut(s) 655
BspLI GGNNCC 5 cut(s) 330, 522, 838, 931, 966
BspMAI CTGCAG 2 cut(s) 464, 532
BspMI ACCTGC 2 cut(s) 109, 952
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 2 cut(s) 328, 964
BspTNI GGTCTC 2 cut(s) 459, 789
BsrDI GCAATG 1 cut(s) 81
BssECI CCNNGG 4 cut(s) 415, 472, 838, 925
BssMI GATC 3 cut(s) 231, 451, 645
BssT1I CCWWGG 2 cut(s) 472, 925
Bst2UI CCWGG 2 cut(s) 417, 479
Bst4CI ACNGT 2 cut(s) 514, 736
Bst6I CTCTTC 1 cut(s) 427
BstBAI YACGTR 1 cut(s) 706
BstC8I GCNNGC 4 cut(s) 186, 287, 378, 398
BstDEI CTNAG 3 cut(s) 208, 865, 912
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 512, 803
BstKTI GATC 3 cut(s) 234, 454, 648
BstMAI GTCTC 6 cut(s) 123, 313, 459, 789, 823, 998
BstMBI GATC 3 cut(s) 231, 451, 645
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 8 cut(s) 83, 146, 194, 282, 527, 728, 840, 861
BstNI CCWGG 2 cut(s) 417, 479
BstNSI RCATGY 1 cut(s) 204
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 4 cut(s) 415, 477, 502, 838
BstSFI CTRYAG 2 cut(s) 460, 528
BstV1I GCAGC 3 cut(s) 508, 570, 873
BstV2I GAAGAC 1 cut(s) 262
BsuRI GGCC 4 cut(s) 414, 477, 521, 837
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 2 cut(s) 512, 803
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 151, 510, 537, 672, 1031
BveI ACCTGC 2 cut(s) 109, 952
Cac8I GCNNGC 4 cut(s) 186, 287, 378, 398
CaiI CAGNNNCTG 1 cut(s) 215
Cfr13I GGNCC 4 cut(s) 278, 413, 520, 836
Csp6I GTAC 3 cut(s) 482, 823, 965
CviAII CATG 7 cut(s) 201, 282, 348, 473, 599, 905, 957
CviQI GTAC 3 cut(s) 482, 823, 965
DdeI CTNAG 3 cut(s) 208, 865, 912
DpnI GATC 3 cut(s) 233, 453, 647
DpnII GATC 3 cut(s) 231, 451, 645
DraIII CACNNNGTG 1 cut(s) 706
Eam1104I CTCTTC 1 cut(s) 427
EarI CTCTTC 1 cut(s) 427
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 944
Eco130I CCWWGG 2 cut(s) 472, 925
Eco24I GRGCYC 2 cut(s) 402, 946
Eco31I GGTCTC 2 cut(s) 459, 789
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 944
Eco57I CTGAAG 2 cut(s) 363, 1007
EcoICRI GAGCTC 1 cut(s) 944
EcoRI GAATTC 2 cut(s) 624, 681
EcoRII CCWGG 2 cut(s) 415, 477
EcoT14I CCWWGG 2 cut(s) 472, 925
EcoT38I GRGCYC 2 cut(s) 402, 946
ErhI CCWWGG 2 cut(s) 472, 925
Esp3I CGTCTC 1 cut(s) 823
FaeI CATG 7 cut(s) 204, 285, 351, 476, 602, 908, 960
FatI CATG 7 cut(s) 200, 281, 347, 472, 598, 904, 956
FauI CCCGC 1 cut(s) 389
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 6 cut(s) 78, 497, 500, 559, 835, 862
FokI GGATG 2 cut(s) 519, 790
FriOI GRGCYC 2 cut(s) 402, 946
Fsp4HI GCNGC 6 cut(s) 78, 497, 500, 559, 835, 862
FspBI CTAG 2 cut(s) 314, 377
GluI GCNGC 6 cut(s) 78, 497, 500, 559, 835, 862
HaeIII GGCC 4 cut(s) 414, 477, 521, 837
HapII CCGG 5 cut(s) 150, 263, 504, 656, 840
Hin1II CATG 7 cut(s) 204, 285, 351, 476, 602, 908, 960
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 5 cut(s) 212, 390, 423, 849, 948
HpaII CCGG 5 cut(s) 150, 263, 504, 656, 840
HphI GGTGA 1 cut(s) 330
Hpy166II GTNNAC 2 cut(s) 739, 919
Hpy188I TCNGA 6 cut(s) 128, 137, 172, 211, 217, 990
Hpy188III TCNNGA 3 cut(s) 656, 952, 1001
Hpy8I GTNNAC 2 cut(s) 739, 919
Hpy99I CGWCG 1 cut(s) 246
HpyAV CCTTC 3 cut(s) 346, 605, 614
HpyCH4III ACNGT 2 cut(s) 514, 736
HpyCH4IV ACGT 2 cut(s) 705, 825
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 146, 194, 282, 527, 728, 840, 861
HpyF3I CTNAG 3 cut(s) 208, 865, 912
HpySE526I ACGT 2 cut(s) 705, 825
Hsp92II CATG 7 cut(s) 204, 285, 351, 476, 602, 908, 960
Kpn2I TCCGGA 1 cut(s) 655
KpnI GGTACC 1 cut(s) 968
Kzo9I GATC 3 cut(s) 231, 451, 645
LmnI GCTCC 4 cut(s) 137, 355, 658, 941
Lsp1109I GCAGC 3 cut(s) 508, 570, 873
LweI GCATC 1 cut(s) 497
MaeI CTAG 2 cut(s) 314, 377
MaeII ACGT 2 cut(s) 705, 825
MaeIII GTNAC 3 cut(s) 548, 712, 907
MalI GATC 3 cut(s) 233, 453, 647
MboI GATC 3 cut(s) 231, 451, 645
MhlI GDGCHC 2 cut(s) 402, 946
MlyI GAGTC 1 cut(s) 399
MnlI CCTC 6 cut(s) 146, 428, 562, 706, 864, 932
MroI TCCGGA 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 5 cut(s) 258, 605, 755, 767, 828
MspA1I CMGCKG 1 cut(s) 396
MspI CCGG 5 cut(s) 150, 263, 504, 656, 840
MspR9I CCNGG 4 cut(s) 417, 479, 504, 840
Mva1269I GAATGC 2 cut(s) 176, 199
MvaI CCWGG 2 cut(s) 417, 479
MwoI GCNNNNNNNGC 8 cut(s) 83, 146, 194, 282, 527, 728, 840, 861
NciI CCSGG 2 cut(s) 504, 840
NcoI CCATGG 1 cut(s) 472
NdeII GATC 3 cut(s) 231, 451, 645
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 7 cut(s) 204, 285, 351, 476, 602, 908, 960
NlaIV GGNNCC 5 cut(s) 330, 522, 838, 931, 966
NmuCI GTSAC 1 cut(s) 712
NspI RCATGY 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PctI GAATGC 2 cut(s) 176, 199
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 4 cut(s) 212, 423, 849, 948
Pfl23II CGTACG 1 cut(s) 822
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 6 cut(s) 79, 498, 501, 560, 836, 863
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Ppu21I YACGTR 1 cut(s) 706
PshAI GACNNNNGTC 1 cut(s) 366
PshBI ATTAAT 1 cut(s) 755
Psp124BI GAGCTC 1 cut(s) 946
Psp6I CCWGG 2 cut(s) 415, 477
PspGI CCWGG 2 cut(s) 415, 477
PspLI CGTACG 1 cut(s) 822
PspN4I GGNNCC 5 cut(s) 330, 522, 838, 931, 966
PspPI GGNCC 4 cut(s) 278, 413, 520, 836
PstI CTGCAG 2 cut(s) 464, 532
PstNI CAGNNNCTG 1 cut(s) 215
RsaI GTAC 3 cut(s) 483, 824, 966
RsaNI GTAC 3 cut(s) 482, 823, 965
SacI GAGCTC 1 cut(s) 946
SaqAI TTAA 5 cut(s) 258, 605, 755, 767, 828
SatI GCNGC 6 cut(s) 78, 497, 500, 559, 835, 862
Sau3AI GATC 3 cut(s) 231, 451, 645
Sau96I GGNCC 4 cut(s) 278, 413, 520, 836
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 4 cut(s) 417, 479, 504, 840
SduI GDGCHC 2 cut(s) 402, 946
SfaNI GCATC 1 cut(s) 497
SfcI CTRYAG 2 cut(s) 460, 528
SinI GGWCC 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 401, 591
SmoI CTYRAG 2 cut(s) 401, 591
SsiI CCGC 6 cut(s) 75, 78, 276, 396, 500, 834
SspMI CTAG 2 cut(s) 314, 377
SstI GAGCTC 1 cut(s) 946
StyD4I CCNGG 4 cut(s) 415, 477, 502, 838
StyI CCWWGG 2 cut(s) 472, 925
TaaI ACNGT 2 cut(s) 514, 736
TaiI ACGT 2 cut(s) 708, 828
TaqI TCGA 3 cut(s) 648, 946, 1000
TauI GCSGC 3 cut(s) 80, 502, 837
TfiI GAWTC 4 cut(s) 212, 423, 849, 948
Tru1I TTAA 5 cut(s) 258, 605, 755, 767, 828
Tru9I TTAA 5 cut(s) 258, 605, 755, 767, 828
TscAI CASTG 5 cut(s) 151, 517, 537, 679, 1031
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 3 cut(s) 496, 558, 861
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 4 cut(s) 563, 753, 893, 987
TspGWI ACGGA 4 cut(s) 259, 693, 861, 954
TspRI CASTG 5 cut(s) 151, 517, 537, 679, 1031
Van91I CCANNNNNTGG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 8 cut(s) 219, 248, 301, 624, 681, 880, 983, 996
XceI RCATGY 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 918
XmnI GAANNNNTTC 1 cut(s) 624
XspI CTAG 2 cut(s) 314, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.