Rh2DG038900

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014535
Physical Location & Seq
Forward (+)
2712657 .. 2715053
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG038900.1

Sequence Viewer

Length: 1227 bp
ATGGGGAGCTATCTTTTTCTAGTTTTTCTTGGGACAGTTTCTGTGTTTACTGCATGTTCAGAACATGAACATGCAAACGCTACAATGGCTACTTTTAATGTCTTGGACTATGGAGCTGTCAGTGGTGGCCACATAGACAATGCACTGGCATTCTTGGATGCATGGAATGCAACATGTAGCTCAGAAGCTGAAAACTCGACTCTAATCATTCCCGGAAGGAGATTTCTTGTAAATCCAACCACTTTCAATGGTCCATGCAAAGCCCAAAGCATCAATTTTGTGATATTAGGGAGACTTTTGGCACCAAAGTCACCTGAAGCATGGAAGGACCTTGATCCAGGTCAATGGCTAGCATTTCATGGTGTTAATGGACTCATGGTTGCTGGCCCTGGTACTATTAATGGAAGAGGAAAGCGCTGGTGGAATCAATCTTGCAGATATCATCCAAAACTGGAGGGATGCACTTCACGGGCTCCAACGGCTTTGAAGGTTCTATCATGCAAGAAAAGCTGTCTTAGAGATATTCACTTTATAAACAGCTCCCAAGCCCATGTCTTAATAGAAGGGTGTGATGGAATCAAGATTGAGAATCTTATGATTGAAGCTCCAGGAAATAGTCCCAACACTGATGGGATTCACATTCAGTCCTCTCAAAATGTCATTATCAACAACACCATCATAGGCTCTGGTGATGATTGTATATCAATTGGAGACCATATATCCAATATTGCCATCTCATATATTAAATGTGGCCCTGGTCATGGAATAAGCATTGGAAGCTTAGGGAAATCCGGGAATGTCGTTCATGTAGTGAACATTCATGTCAGTAAGGTTACCCTCCAAGGAACTACGAACGGAGCTCGGATCAAGACATGGCAGGTTGGCAGGGGTTCTGTTCGAGGGGTCAGATTCGAAGATTTCTTTCTGGAGTCTGTTAAGAACCCCATAATCATCGATCAAAACTACTGCGAAGTTAGGGGTGCCTGCGAGGAACTGCCGACTGGTGTTCACATAAGTGATGTCTCCTTCAAAAACTTCTATGGAACATCGAGTACCAATATCGCCATCAATCTGAATTGCAGTCGCTCCGTTCCTTGCACCGGAATATTGTTGAAGTCGGTATACCTTCGATCTGCTATAGTTGGACAGAATGTAACTTCTAGTTGCAGAAATGCCTACGGCATTGCCTTTGGATTAGTTCAGCCAAGCCCCTGTGTCCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

408

Amino Acids

43.83

Weight (kDa)

8.3

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 392 3.8e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80140
fragaria_vesca FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310
prunus_persica Prupe.7G242600_v2.0.a1
pyrus_communis pycom15g15490
rosa_chinensis RchiOBHm_Chr2g0088471
rosa_laevigata RLG00000015957
rosa_roxburghii Rroxscaffold_2G00152660
rosa_rugosa Rorug01G0484300
rosa_samantha Rh2AG039400 Rh2BG038200 Rh2CG039500 Rh2DG038900
rosa_wichuraiana Rw2G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 868
AccB1I GGYRCC 2 cut(s) 301, 980
AccI GTMKAC 1 cut(s) 1122
AclWI GGATC 2 cut(s) 329, 872
AcoI YGGCCR 1 cut(s) 127
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 2 cut(s) 394, 1054
AfeI AGCGCT 1 cut(s) 416
AfiI CCNNNNNNNGG 2 cut(s) 761, 1100
AflIII ACRYGT 1 cut(s) 173
AgsI TTSAA 5 cut(s) 247, 487, 602, 1030, 1114
AjnI CCWGG 4 cut(s) 337, 388, 607, 754
AleI CACNNNNGTG 1 cut(s) 1014
AluBI AGCT 9 cut(s) 9, 116, 180, 188, 510, 540, 605, 780, 860
AluI AGCT 9 cut(s) 9, 116, 180, 188, 510, 540, 605, 780, 860
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 3 cut(s) 286, 705, 1027
AlwI GGATC 2 cut(s) 329, 872
AlwNI CAGNNNCTG 2 cut(s) 41, 188
Aor51HI AGCGCT 1 cut(s) 416
AoxI GGCC 3 cut(s) 127, 385, 751
AseI ATTAAT 1 cut(s) 399
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 251, 328, 386, 752
AsuC2I CCSGG 2 cut(s) 213, 793
AsuHPI GGTGA 2 cut(s) 303, 701
AsuII TTCGAA 1 cut(s) 912
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 2 cut(s) 251, 328
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 2 cut(s) 301, 980
BanII GRGCYC 2 cut(s) 475, 862
BarI GAAGNNNNNNTAC 2 cut(s) 1106, 1138
Bbv12I GWGCWC 1 cut(s) 862
BccI CCATC 5 cut(s) 566, 623, 683, 740, 1073
BceAI ACGGC 2 cut(s) 495, 1195
BciT130I CCWGG 4 cut(s) 339, 390, 609, 756
BcnI CCSGG 2 cut(s) 213, 793
BcoDI GTCTC 3 cut(s) 286, 705, 1027
BfaI CTAG 3 cut(s) 20, 350, 1161
BfmI CTRYAG 1 cut(s) 1137
BfoI RGCGCY 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 868
Bme1390I CCNGG 6 cut(s) 213, 339, 390, 609, 756, 793
Bme18I GGWCC 2 cut(s) 251, 328
BmgT120I GGNCC 4 cut(s) 251, 328, 386, 752
BmiI GGNNCC 3 cut(s) 303, 474, 982
BmrFI CCNGG 6 cut(s) 213, 339, 390, 609, 756, 793
BmsI GCATC 3 cut(s) 148, 279, 449
BmtI GCTAGC 1 cut(s) 353
BpmI CTGGAG 3 cut(s) 473, 591, 947
Bpu10I CCTNAGC 1 cut(s) 781
Bpu14I TTCGAA 1 cut(s) 912
BpuMI CCSGG 2 cut(s) 213, 793
Bsa29I ATCGAT 1 cut(s) 954
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 3 cut(s) 388, 754, 841
BsaWI WCCGGW 1 cut(s) 1100
Bsc4I CCNNNNNNNGG 2 cut(s) 761, 1100
Bse1I ACTGG 3 cut(s) 150, 456, 1006
Bse3DI GCAATG 1 cut(s) 1182
BseBI CCWGG 4 cut(s) 339, 390, 609, 756
BseCI ATCGAT 1 cut(s) 954
BseDI CCNNGG 3 cut(s) 388, 754, 841
BseGI GGATG 3 cut(s) 163, 442, 464
BseLI CCNNNNNNNGG 2 cut(s) 761, 1100
BseMI GCAATG 1 cut(s) 1182
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 3 cut(s) 150, 456, 1006
BshFI GGCC 3 cut(s) 129, 387, 753
BshNI GGYRCC 2 cut(s) 301, 980
BshVI ATCGAT 1 cut(s) 954
BsiHKAI GWGCWC 1 cut(s) 862
BsiSI CCGG 3 cut(s) 213, 792, 1101
BslFI GGGAC 2 cut(s) 46, 603
BslI CCNNNNNNNGG 2 cut(s) 761, 1100
BsmAI GTCTC 3 cut(s) 286, 705, 1027
BsmFI GGGAC 2 cut(s) 46, 603
BsmI GAATGC 2 cut(s) 149, 172
BsnI GGCC 3 cut(s) 129, 387, 753
Bso31I GGTCTC 1 cut(s) 705
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 2 cut(s) 475, 862
Bsp143I GATC 4 cut(s) 334, 864, 955, 1130
BspANI GGCC 3 cut(s) 129, 387, 753
BspCNI CTCAG 1 cut(s) 194
BspDI ATCGAT 1 cut(s) 954
BspLI GGNNCC 3 cut(s) 303, 474, 982
BspMI ACCTGC 1 cut(s) 868
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 329, 872
BspT104I TTCGAA 1 cut(s) 912
BspT107I GGYRCC 2 cut(s) 301, 980
BspTNI GGTCTC 1 cut(s) 705
BsrDI GCAATG 1 cut(s) 1182
BsrI ACTGG 3 cut(s) 150, 456, 1006
BssECI CCNNGG 3 cut(s) 388, 754, 841
BssMI GATC 4 cut(s) 334, 864, 955, 1130
BssNAI GTATAC 1 cut(s) 1123
BssT1I CCWWGG 1 cut(s) 841
Bst1107I GTATAC 1 cut(s) 1123
Bst2UI CCWGG 4 cut(s) 339, 390, 609, 756
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 3 cut(s) 351, 385, 985
BstDEI CTNAG 3 cut(s) 181, 515, 781
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 3 cut(s) 163, 442, 464
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 4 cut(s) 337, 867, 958, 1133
BstMAI GTCTC 3 cut(s) 286, 705, 1027
BstMBI GATC 4 cut(s) 334, 864, 955, 1130
BstMWI GCNNNNNNNGC 5 cut(s) 86, 167, 479, 507, 777
BstNI CCWGG 4 cut(s) 339, 390, 609, 756
BstNSI RCATGY 3 cut(s) 57, 74, 177
BstPI GGTNACC 1 cut(s) 832
BstSCI CCNGG 6 cut(s) 211, 337, 388, 607, 754, 791
BstSFI CTRYAG 1 cut(s) 1137
BstXI CCANNNNNNTGG 1 cut(s) 345
BstZ17I GTATAC 1 cut(s) 1123
Bsu15I ATCGAT 1 cut(s) 954
BsuRI GGCC 3 cut(s) 129, 387, 753
BsuTUI ATCGAT 1 cut(s) 954
BtsCI GGATG 3 cut(s) 163, 442, 464
BtsIMutI CAGTG 3 cut(s) 127, 143, 624
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 3 cut(s) 351, 385, 985
CaiI CAGNNNCTG 2 cut(s) 41, 188
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 4 cut(s) 251, 328, 386, 752
ClaI ATCGAT 1 cut(s) 954
Csp6I GTAC 2 cut(s) 393, 1053
CviQI GTAC 2 cut(s) 393, 1053
DdeI CTNAG 3 cut(s) 181, 515, 781
DpnI GATC 4 cut(s) 336, 866, 957, 1132
DpnII GATC 4 cut(s) 334, 864, 955, 1130
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 841
Eco24I GRGCYC 2 cut(s) 475, 862
Eco31I GGTCTC 1 cut(s) 705
Eco32I GATATC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 251, 328
Eco47III AGCGCT 1 cut(s) 416
Eco53kI GAGCTC 1 cut(s) 860
Eco57I CTGAAG 1 cut(s) 336
Eco91I GGTNACC 1 cut(s) 832
EcoICRI GAGCTC 1 cut(s) 860
EcoO109I RGGNCCY 1 cut(s) 328
EcoO65I GGTNACC 1 cut(s) 832
EcoRII CCWGG 4 cut(s) 337, 388, 607, 754
EcoRV GATATC 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 841
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 2 cut(s) 475, 862
ErhI CCWWGG 1 cut(s) 841
FaqI GGGAC 2 cut(s) 46, 603
FblI GTMKAC 1 cut(s) 1122
FokI GGATG 3 cut(s) 170, 429, 471
FriOI GRGCYC 2 cut(s) 475, 862
FspBI CTAG 3 cut(s) 20, 350, 1161
GlaI GCGC 1 cut(s) 416
GsuI CTGGAG 3 cut(s) 473, 591, 947
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 3 cut(s) 129, 387, 753
HapII CCGG 3 cut(s) 213, 792, 1101
HhaI GCGC 1 cut(s) 417
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 778
HinfI GANTC 8 cut(s) 199, 372, 424, 576, 589, 634, 909, 929
HpaII CCGG 3 cut(s) 213, 792, 1101
HphI GGTGA 2 cut(s) 303, 701
Hpy166II GTNNAC 4 cut(s) 48, 814, 1009, 1123
Hpy188I TCNGA 6 cut(s) 61, 184, 864, 908, 1074, 1226
Hpy188III TCNNGA 3 cut(s) 580, 868, 926
Hpy8I GTNNAC 4 cut(s) 48, 814, 1009, 1123
HpyAV CCTTC 6 cut(s) 210, 319, 481, 557, 1036, 1136
HpyCH4III ACNGT 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 167, 479, 507, 777
HpyF3I CTNAG 3 cut(s) 181, 515, 781
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 4 cut(s) 334, 864, 955, 1130
LmnI GCTCC 7 cut(s) 6, 113, 478, 545, 610, 857, 1091
LweI GCATC 3 cut(s) 148, 279, 449
MaeI CTAG 3 cut(s) 20, 350, 1161
MaeIII GTNAC 3 cut(s) 309, 832, 1153
MalI GATC 4 cut(s) 336, 866, 957, 1132
MboI GATC 4 cut(s) 334, 864, 955, 1130
MboII GAAGA 2 cut(s) 417, 926
MfeI CAATTG 1 cut(s) 705
MhlI GDGCHC 2 cut(s) 475, 862
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 4 cut(s) 274, 705, 1075, 1220
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 3 cut(s) 193, 366, 938
MmeI TCCRAC 3 cut(s) 260, 500, 1123
MnlI CCTC 6 cut(s) 401, 448, 658, 848, 893, 982
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 163
MscI TGGCCA 1 cut(s) 129
MseI TTAA 6 cut(s) 96, 366, 399, 557, 744, 936
MslI CAYNNNNRTG 2 cut(s) 69, 1014
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 3 cut(s) 213, 792, 1101
MspR9I CCNGG 6 cut(s) 213, 339, 390, 609, 756, 793
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 2 cut(s) 149, 172
MvaI CCWGG 4 cut(s) 339, 390, 609, 756
MwoI GCNNNNNNNGC 5 cut(s) 86, 167, 479, 507, 777
NciI CCSGG 2 cut(s) 213, 793
NdeII GATC 4 cut(s) 334, 864, 955, 1130
NheI GCTAGC 1 cut(s) 349
NlaIV GGNNCC 3 cut(s) 303, 474, 982
NmuCI GTSAC 1 cut(s) 309
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 3 cut(s) 57, 74, 177
NspV TTCGAA 1 cut(s) 912
OliI CACNNNNGTG 1 cut(s) 1014
PciI ACATGT 1 cut(s) 173
PctI GAATGC 2 cut(s) 149, 172
PfeI GAWTC 5 cut(s) 424, 576, 589, 634, 909
PfoI TCCNGGA 3 cut(s) 211, 607, 791
PleI GAGTC 3 cut(s) 193, 366, 937
PpsI GAGTC 3 cut(s) 193, 366, 937
PpuMI RGGWCCY 1 cut(s) 328
PscI ACATGT 1 cut(s) 173
PshBI ATTAAT 1 cut(s) 399
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 862
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 4 cut(s) 337, 388, 607, 754
PspEI GGTNACC 1 cut(s) 832
PspGI CCWGG 4 cut(s) 337, 388, 607, 754
PspN4I GGNNCC 3 cut(s) 303, 474, 982
PspPI GGNCC 4 cut(s) 251, 328, 386, 752
PspPPI RGGWCCY 1 cut(s) 328
PsrI GAACNNNNNNTAC 2 cut(s) 1036, 1068
PstNI CAGNNNCTG 2 cut(s) 41, 188
RsaI GTAC 2 cut(s) 394, 1054
RsaNI GTAC 2 cut(s) 393, 1053
RseI CAYNNNNRTG 2 cut(s) 69, 1014
SacI GAGCTC 1 cut(s) 862
SaqAI TTAA 6 cut(s) 96, 366, 399, 557, 744, 936
Sau3AI GATC 4 cut(s) 334, 864, 955, 1130
Sau96I GGNCC 4 cut(s) 251, 328, 386, 752
SchI GAGTC 3 cut(s) 193, 366, 938
ScrFI CCNGG 6 cut(s) 213, 339, 390, 609, 756, 793
SduI GDGCHC 2 cut(s) 475, 862
SfaNI GCATC 3 cut(s) 148, 279, 449
SfcI CTRYAG 1 cut(s) 1137
SfuI TTCGAA 1 cut(s) 912
SinI GGWCC 2 cut(s) 251, 328
SmiMI CAYNNNNRTG 2 cut(s) 69, 1014
Sse9I AATT 4 cut(s) 274, 705, 1075, 1220
SspI AATATT 2 cut(s) 727, 1107
SspMI CTAG 3 cut(s) 20, 350, 1161
SstI GAGCTC 1 cut(s) 862
StyD4I CCNGG 6 cut(s) 211, 337, 388, 607, 754, 791
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 6 cut(s) 197, 898, 912, 954, 1049, 1129
TasI AATT 4 cut(s) 274, 705, 1075, 1220
TfiI GAWTC 5 cut(s) 424, 576, 589, 634, 909
Tru1I TTAA 6 cut(s) 96, 366, 399, 557, 744, 936
Tru9I TTAA 6 cut(s) 96, 366, 399, 557, 744, 936
TscAI CASTG 3 cut(s) 127, 150, 631
TseFI GTSAC 1 cut(s) 309
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 4 cut(s) 81, 347, 794, 809
TspGWI ACGGA 2 cut(s) 870, 1078
TspRI CASTG 3 cut(s) 127, 150, 631
VpaK11BI GGWCC 2 cut(s) 251, 328
VspI ATTAAT 1 cut(s) 399
XceI RCATGY 3 cut(s) 57, 74, 177
XmiI GTMKAC 1 cut(s) 1122
XspI CTAG 3 cut(s) 20, 350, 1161
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.