Rw2G003090

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014535
Physical Location & Seq
Forward (+)
2936958 .. 2938988
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003090.1

Sequence Viewer

Length: 1236 bp
ATGCATTTGCAGGGGAGCTATCTTTTTCTAGTTTTTCTTGGGACAGTTTCTGTGTTTACTGCATGTTCAGAACATGAACATGCAAACGCTACAATGGCTACTTTTAATGTCTTGGACTATGGAGCTGTCAGTGGTGGCCACATAGACAATGCACTGGCATTCTTGGATGCATGGAATGCAACATGTAGCTCAGAAGCTGAAAACTCGACTCTAATCATTCCCGGAAGGAGATTTCTTGTAAATCCAACCACTTTCAATGGTCCATGCAAAGCCCAAAGCATCAATTTTGTGATATTAGGGAGACTTTTGGCACCAAAGTCACCTGAAGCATGGAAGGACCTTGATCCAGGTCAATGGCTAGCATTTCATGGTGTTAATGGACTCATGGTTGCTGGCCCTGGTACTATTAATGGAAGAGGAAAGCGCTGGTGGAATCAATCTTGCAGATATCATCCAAAACTGGAGGGATGCACTTCACGGGCTCCAACGGCTTTGAAGGTTCTATCATGCAAGAAAAGCTGTCTTAGAGATATTCACTTTATAAACAGCTCCCAAGCCCATGTCTTAATAGAAGGGTGTGATGGAATCAAGATTGAGAATCTTATGATTGAAGCTCCAGGAAATAGTCCCAACACTGATGGGATTCACATTCAGTCCTCTCAAAATGTCATTATCAACAACACCATCATAGGCTCTGGTGATGATTGTATATCAATTGGAGACCATATATCCAATATTGCCATCTCATATATTAAATGTGGCCCTGGTCATGGAATAAGCATTGGAAGCTTAGGGAAATCCGGGAATGTCGTTCATGTAGTGAACATTCATGTCAGTAAGGTTACCCTCCAAGGAACTACGAACGGAGCTCGGATCAAGACATGGCAGGTTGGCAGGGGTTCTGTTCGAGGGGTCAGATTCGAAGATTTCTTTCTGGAGTCTGTTAAGAACCCCATAATCATCGATCAAAACTACTGCGAAGTTAGGGGTGCCTGCGAGGAACTGCCGACTGGTGTTCACATAAGTGATGTCTCCTTCAAAAACTTCTATGGAACATCGAGTACCAATATCGCCATCAATCTGAATTGCAGTCGCTCCGTTCCTTGCACCGGAATATTGTTGAAGTCGGTATACCTTCGATCTGCTATAGTTGGACAGAATGTAACTTCTAGTTGCAGAAATGCCTACGGCATTGCCTTTGGATTAGTTCGGCCAAGCCCCTGTGTCCAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

411

Amino Acids

44.24

Weight (kDa)

8.44

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 65 - 395 3.9e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80140
fragaria_vesca FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310
prunus_persica Prupe.7G242600_v2.0.a1
pyrus_communis pycom15g15490
rosa_chinensis RchiOBHm_Chr2g0088471
rosa_laevigata RLG00000015957
rosa_roxburghii Rroxscaffold_2G00152660
rosa_rugosa Rorug01G0484300
rosa_samantha Rh2AG039400 Rh2BG038200 Rh2CG039500 Rh2DG038900
rosa_wichuraiana Rw2G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 877
AccB1I GGYRCC 2 cut(s) 310, 989
AccI GTMKAC 1 cut(s) 1131
AclWI GGATC 2 cut(s) 338, 881
AcoI YGGCCR 2 cut(s) 136, 1211
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 2 cut(s) 403, 1063
AfeI AGCGCT 1 cut(s) 425
AfiI CCNNNNNNNGG 2 cut(s) 770, 1109
AflIII ACRYGT 1 cut(s) 182
AgsI TTSAA 5 cut(s) 256, 496, 611, 1039, 1123
AjnI CCWGG 4 cut(s) 346, 397, 616, 763
AleI CACNNNNGTG 1 cut(s) 1023
AluBI AGCT 9 cut(s) 18, 125, 189, 197, 519, 549, 614, 789, 869
AluI AGCT 9 cut(s) 18, 125, 189, 197, 519, 549, 614, 789, 869
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 3 cut(s) 295, 714, 1036
AlwI GGATC 2 cut(s) 338, 881
AlwNI CAGNNNCTG 2 cut(s) 50, 197
Aor51HI AGCGCT 1 cut(s) 425
AoxI GGCC 4 cut(s) 136, 394, 760, 1211
AseI ATTAAT 1 cut(s) 408
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 4 cut(s) 260, 337, 395, 761
AsuC2I CCSGG 2 cut(s) 222, 802
AsuHPI GGTGA 2 cut(s) 312, 710
AsuII TTCGAA 1 cut(s) 921
AsuNHI GCTAGC 1 cut(s) 358
AvaII GGWCC 2 cut(s) 260, 337
BalI TGGCCA 1 cut(s) 138
BanI GGYRCC 2 cut(s) 310, 989
BanII GRGCYC 2 cut(s) 484, 871
BarI GAAGNNNNNNTAC 2 cut(s) 1115, 1147
Bbv12I GWGCWC 1 cut(s) 871
BccI CCATC 5 cut(s) 575, 632, 692, 749, 1082
BceAI ACGGC 2 cut(s) 504, 1204
BciT130I CCWGG 4 cut(s) 348, 399, 618, 765
BcnI CCSGG 2 cut(s) 222, 802
BcoDI GTCTC 3 cut(s) 295, 714, 1036
BfaI CTAG 3 cut(s) 29, 359, 1170
BfmI CTRYAG 1 cut(s) 1146
BfoI RGCGCY 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 877
Bme1390I CCNGG 6 cut(s) 222, 348, 399, 618, 765, 802
Bme18I GGWCC 2 cut(s) 260, 337
BmgT120I GGNCC 4 cut(s) 260, 337, 395, 761
BmiI GGNNCC 3 cut(s) 312, 483, 991
BmrFI CCNGG 6 cut(s) 222, 348, 399, 618, 765, 802
BmsI GCATC 3 cut(s) 157, 288, 458
BmtI GCTAGC 1 cut(s) 362
BpmI CTGGAG 3 cut(s) 482, 600, 956
Bpu10I CCTNAGC 1 cut(s) 790
Bpu14I TTCGAA 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 222, 802
Bsa29I ATCGAT 1 cut(s) 963
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 3 cut(s) 397, 763, 850
BsaWI WCCGGW 1 cut(s) 1109
Bsc4I CCNNNNNNNGG 2 cut(s) 770, 1109
Bse1I ACTGG 3 cut(s) 159, 465, 1015
Bse3DI GCAATG 1 cut(s) 1191
BseBI CCWGG 4 cut(s) 348, 399, 618, 765
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 3 cut(s) 397, 763, 850
BseGI GGATG 3 cut(s) 172, 451, 473
BseLI CCNNNNNNNGG 2 cut(s) 770, 1109
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 3 cut(s) 159, 465, 1015
BshFI GGCC 4 cut(s) 138, 396, 762, 1213
BshNI GGYRCC 2 cut(s) 310, 989
BshVI ATCGAT 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 871
BsiSI CCGG 3 cut(s) 222, 801, 1110
BslFI GGGAC 2 cut(s) 55, 612
BslI CCNNNNNNNGG 2 cut(s) 770, 1109
BsmAI GTCTC 3 cut(s) 295, 714, 1036
BsmFI GGGAC 2 cut(s) 55, 612
BsmI GAATGC 2 cut(s) 158, 181
BsnI GGCC 4 cut(s) 138, 396, 762, 1213
Bso31I GGTCTC 1 cut(s) 714
Bsp119I TTCGAA 1 cut(s) 921
Bsp1286I GDGCHC 2 cut(s) 484, 871
Bsp143I GATC 4 cut(s) 343, 873, 964, 1139
BspANI GGCC 4 cut(s) 138, 396, 762, 1213
BspCNI CTCAG 1 cut(s) 203
BspDI ATCGAT 1 cut(s) 963
BspLI GGNNCC 3 cut(s) 312, 483, 991
BspMI ACCTGC 1 cut(s) 877
BspOI GCTAGC 1 cut(s) 362
BspPI GGATC 2 cut(s) 338, 881
BspT104I TTCGAA 1 cut(s) 921
BspT107I GGYRCC 2 cut(s) 310, 989
BspTNI GGTCTC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 1191
BsrI ACTGG 3 cut(s) 159, 465, 1015
BssECI CCNNGG 3 cut(s) 397, 763, 850
BssMI GATC 4 cut(s) 343, 873, 964, 1139
BssNAI GTATAC 1 cut(s) 1132
BssT1I CCWWGG 1 cut(s) 850
Bst1107I GTATAC 1 cut(s) 1132
Bst2UI CCWGG 4 cut(s) 348, 399, 618, 765
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 409
BstAPI GCANNNNNTGC 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 921
BstC8I GCNNGC 3 cut(s) 360, 394, 994
BstDEI CTNAG 3 cut(s) 190, 524, 790
BstEII GGTNACC 1 cut(s) 841
BstF5I GGATG 3 cut(s) 172, 451, 473
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 4 cut(s) 346, 876, 967, 1142
BstMAI GTCTC 3 cut(s) 295, 714, 1036
BstMBI GATC 4 cut(s) 343, 873, 964, 1139
BstMWI GCNNNNNNNGC 5 cut(s) 95, 176, 488, 516, 786
BstNI CCWGG 4 cut(s) 348, 399, 618, 765
BstNSI RCATGY 3 cut(s) 66, 83, 186
BstPI GGTNACC 1 cut(s) 841
BstSCI CCNGG 6 cut(s) 220, 346, 397, 616, 763, 800
BstSFI CTRYAG 1 cut(s) 1146
BstXI CCANNNNNNTGG 1 cut(s) 354
BstZ17I GTATAC 1 cut(s) 1132
Bsu15I ATCGAT 1 cut(s) 963
BsuRI GGCC 4 cut(s) 138, 396, 762, 1213
BsuTUI ATCGAT 1 cut(s) 963
BtsCI GGATG 3 cut(s) 172, 451, 473
BtsIMutI CAGTG 3 cut(s) 136, 152, 633
BveI ACCTGC 1 cut(s) 877
Cac8I GCNNGC 3 cut(s) 360, 394, 994
CaiI CAGNNNCTG 2 cut(s) 50, 197
CfoI GCGC 1 cut(s) 426
Cfr13I GGNCC 4 cut(s) 260, 337, 395, 761
ClaI ATCGAT 1 cut(s) 963
Csp6I GTAC 2 cut(s) 402, 1062
CviQI GTAC 2 cut(s) 402, 1062
DdeI CTNAG 3 cut(s) 190, 524, 790
DpnI GATC 4 cut(s) 345, 875, 966, 1141
DpnII GATC 4 cut(s) 343, 873, 964, 1139
EaeI YGGCCR 2 cut(s) 136, 1211
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Ecl136II GAGCTC 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 484, 871
Eco31I GGTCTC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 260, 337
Eco47III AGCGCT 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 869
Eco57I CTGAAG 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 841
EcoICRI GAGCTC 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 337
EcoO65I GGTNACC 1 cut(s) 841
EcoRII CCWGG 4 cut(s) 346, 397, 616, 763
EcoRV GATATC 1 cut(s) 449
EcoT14I CCWWGG 1 cut(s) 850
EcoT22I ATGCAT 2 cut(s) 6, 172
EcoT38I GRGCYC 2 cut(s) 484, 871
ErhI CCWWGG 1 cut(s) 850
FaqI GGGAC 2 cut(s) 55, 612
FblI GTMKAC 1 cut(s) 1131
FokI GGATG 3 cut(s) 179, 438, 480
FriOI GRGCYC 2 cut(s) 484, 871
FspBI CTAG 3 cut(s) 29, 359, 1170
GlaI GCGC 1 cut(s) 425
GsuI CTGGAG 3 cut(s) 482, 600, 956
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 4 cut(s) 138, 396, 762, 1213
HapII CCGG 3 cut(s) 222, 801, 1110
HhaI GCGC 1 cut(s) 426
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 787
HinfI GANTC 8 cut(s) 208, 381, 433, 585, 598, 643, 918, 938
HpaII CCGG 3 cut(s) 222, 801, 1110
HphI GGTGA 2 cut(s) 312, 710
Hpy166II GTNNAC 4 cut(s) 57, 823, 1018, 1132
Hpy188I TCNGA 6 cut(s) 70, 193, 873, 917, 1083, 1235
Hpy188III TCNNGA 3 cut(s) 589, 877, 935
Hpy8I GTNNAC 4 cut(s) 57, 823, 1018, 1132
HpyAV CCTTC 6 cut(s) 219, 328, 490, 566, 1045, 1145
HpyCH4III ACNGT 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 176, 488, 516, 786
HpyF3I CTNAG 3 cut(s) 190, 524, 790
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 4 cut(s) 343, 873, 964, 1139
LmnI GCTCC 7 cut(s) 15, 122, 487, 554, 619, 866, 1100
LweI GCATC 3 cut(s) 157, 288, 458
MaeI CTAG 3 cut(s) 29, 359, 1170
MaeIII GTNAC 3 cut(s) 318, 841, 1162
MalI GATC 4 cut(s) 345, 875, 966, 1141
MboI GATC 4 cut(s) 343, 873, 964, 1139
MboII GAAGA 2 cut(s) 426, 935
MfeI CAATTG 1 cut(s) 714
MhlI GDGCHC 2 cut(s) 484, 871
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 4 cut(s) 283, 714, 1084, 1229
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 3 cut(s) 202, 375, 947
MmeI TCCRAC 3 cut(s) 269, 509, 1132
MnlI CCTC 6 cut(s) 410, 457, 667, 857, 902, 991
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 2 cut(s) 6, 172
MscI TGGCCA 1 cut(s) 138
MseI TTAA 6 cut(s) 105, 375, 408, 566, 753, 945
MslI CAYNNNNRTG 2 cut(s) 78, 1023
Msp20I TGGCCA 1 cut(s) 138
MspI CCGG 3 cut(s) 222, 801, 1110
MspR9I CCNGG 6 cut(s) 222, 348, 399, 618, 765, 802
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 2 cut(s) 158, 181
MvaI CCWGG 4 cut(s) 348, 399, 618, 765
MwoI GCNNNNNNNGC 5 cut(s) 95, 176, 488, 516, 786
NciI CCSGG 2 cut(s) 222, 802
NdeII GATC 4 cut(s) 343, 873, 964, 1139
NheI GCTAGC 1 cut(s) 358
NlaIV GGNNCC 3 cut(s) 312, 483, 991
NmuCI GTSAC 1 cut(s) 318
NsiI ATGCAT 2 cut(s) 6, 172
NspI RCATGY 3 cut(s) 66, 83, 186
NspV TTCGAA 1 cut(s) 921
OliI CACNNNNGTG 1 cut(s) 1023
PciI ACATGT 1 cut(s) 182
PctI GAATGC 2 cut(s) 158, 181
PfeI GAWTC 5 cut(s) 433, 585, 598, 643, 918
PfoI TCCNGGA 3 cut(s) 220, 616, 800
PleI GAGTC 3 cut(s) 202, 375, 946
PpsI GAGTC 3 cut(s) 202, 375, 946
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 1 cut(s) 182
PshBI ATTAAT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 4 cut(s) 346, 397, 616, 763
PspEI GGTNACC 1 cut(s) 841
PspGI CCWGG 4 cut(s) 346, 397, 616, 763
PspN4I GGNNCC 3 cut(s) 312, 483, 991
PspPI GGNCC 4 cut(s) 260, 337, 395, 761
PspPPI RGGWCCY 1 cut(s) 337
PsrI GAACNNNNNNTAC 2 cut(s) 1045, 1077
PstNI CAGNNNCTG 2 cut(s) 50, 197
RsaI GTAC 2 cut(s) 403, 1063
RsaNI GTAC 2 cut(s) 402, 1062
RseI CAYNNNNRTG 2 cut(s) 78, 1023
SacI GAGCTC 1 cut(s) 871
SaqAI TTAA 6 cut(s) 105, 375, 408, 566, 753, 945
Sau3AI GATC 4 cut(s) 343, 873, 964, 1139
Sau96I GGNCC 4 cut(s) 260, 337, 395, 761
SchI GAGTC 3 cut(s) 202, 375, 947
ScrFI CCNGG 6 cut(s) 222, 348, 399, 618, 765, 802
SduI GDGCHC 2 cut(s) 484, 871
SfaNI GCATC 3 cut(s) 157, 288, 458
SfcI CTRYAG 1 cut(s) 1146
SfuI TTCGAA 1 cut(s) 921
SinI GGWCC 2 cut(s) 260, 337
SmiMI CAYNNNNRTG 2 cut(s) 78, 1023
Sse9I AATT 4 cut(s) 283, 714, 1084, 1229
SspI AATATT 2 cut(s) 736, 1116
SspMI CTAG 3 cut(s) 29, 359, 1170
SstI GAGCTC 1 cut(s) 871
StyD4I CCNGG 6 cut(s) 220, 346, 397, 616, 763, 800
StyI CCWWGG 1 cut(s) 850
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 6 cut(s) 206, 907, 921, 963, 1058, 1138
TasI AATT 4 cut(s) 283, 714, 1084, 1229
TfiI GAWTC 5 cut(s) 433, 585, 598, 643, 918
Tru1I TTAA 6 cut(s) 105, 375, 408, 566, 753, 945
Tru9I TTAA 6 cut(s) 105, 375, 408, 566, 753, 945
TscAI CASTG 3 cut(s) 136, 159, 640
TseFI GTSAC 1 cut(s) 318
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 4 cut(s) 90, 356, 803, 818
TspGWI ACGGA 2 cut(s) 879, 1087
TspRI CASTG 3 cut(s) 136, 159, 640
VpaK11BI GGWCC 2 cut(s) 260, 337
VspI ATTAAT 1 cut(s) 408
XceI RCATGY 3 cut(s) 66, 83, 186
XmiI GTMKAC 1 cut(s) 1131
XspI CTAG 3 cut(s) 29, 359, 1170
Zsp2I ATGCAT 2 cut(s) 6, 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.