RLG00000015957

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014535
Physical Location & Seq
Forward (+)
2748544 .. 2750475
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015957

Sequence Viewer

Length: 1137 bp
ATGGCTACTTTTAACGTCTTGGACTATGGAGCTGTCAGTGGTGGCCACATAGACAATGCACTGGCATTCTTGGATGCATGGAATGCAACATGTAGCTCAGAAGCTGAAAACTCAACTCTAATAATTCCCAGAAGGAGATTTCTTGTAAATCCAATCACTTTCAATGGTCCATGCAAAGCCCAAAGCATCAATTTTATATTAGGGAGACTTTTGGCACCAAAGTCACCTGAAGCATGGAAGGACCTTGATCCAGGTCAATGGCTAGCATTTCATGGTGTTAATGGACTCAAGGTTGCTGGCCCTGGTACTATTAATGGAAGAGGAAAGCGCTGGTGGAATCAATCTTGCAGATATCATCCAAAACTGGGATGCACTTCACGGGCTCCAACGGCTTTGAAGGTTCTATCATGCAAGAAAAGCTGTCTTAGAGATATTCACTTTATAAACAGCTCCCAAGCCCATGTCTTAATAGAGGGGTGTGATGGAATCAAGATTGAGAATCTTATGATTGAAGCTCCAGGAAATAGTCCCAACACTGATGGGATTCACATTCAGTCCTCTCAAAATGTCATTATCAACAACACCATCATAGGCTCTGGTGATGATTGTATATCAATTGGAGACCATATATCCAATATTGCCATCTCATATATTAAATGTGGCCCTGGTCATGGAATAAGCATTGGAAGCTTAGGGAAATCCGGGAATGTCGTTCATGTAGAGAACATTCATGTCAGTAAGGTTACCCTCCAAGGAACTACGAACGGAGCTCGGATCAAGACATGGCAGGTTGGCAGGGGTTCTGTTCGAGGGGTCAGATTCGAAGATTTCTTTCTGGAGTCTGTTAAGAACCCCATAATCATCGATCAAAACTACTGCGAAGTTAGGGGTGCCTGCGAGGAACTGCCGACTGGTGTTCACATAAGTGATGTCTCCTTCAAAAACTTCTATGGAACATCGAGTACTAATATCGCCATCAATCTGAATTGCAGTCGCTCCGTTCCTTGCACTGGAATATTGTTGAAGTCGGTATACCTTCGATCTGCTATAGTTGGACAGAATGTAACTTCTAGTTGCAGAAATGCCTACGGCATTGCCTTTGGATTAGTTCGGCCAAGCCCCTGTATCCTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

379

Amino Acids

40.75

Weight (kDa)

8.87

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 34 - 362 7.5e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80140
fragaria_vesca FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310
prunus_persica Prupe.7G242600_v2.0.a1
pyrus_communis pycom15g15490
rosa_chinensis RchiOBHm_Chr2g0088471
rosa_laevigata RLG00000015957
rosa_roxburghii Rroxscaffold_2G00152660
rosa_rugosa Rorug01G0484300
rosa_samantha Rh2AG039400 Rh2BG038200 Rh2CG039500 Rh2DG038900
rosa_wichuraiana Rw2G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 2 cut(s) 214, 890
AccI GTMKAC 1 cut(s) 1032
AclWI GGATC 2 cut(s) 242, 782
AcoI YGGCCR 2 cut(s) 43, 1112
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 307, 964
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 3 cut(s) 365, 671, 1010
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 5 cut(s) 163, 397, 512, 940, 1024
AjnI CCWGG 4 cut(s) 250, 301, 517, 664
AleI CACNNNNGTG 1 cut(s) 924
AluBI AGCT 8 cut(s) 32, 96, 104, 420, 450, 515, 690, 770
AluI AGCT 8 cut(s) 32, 96, 104, 420, 450, 515, 690, 770
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 3 cut(s) 199, 615, 937
AlwI GGATC 2 cut(s) 242, 782
AlwNI CAGNNNCTG 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 4 cut(s) 43, 298, 661, 1112
AseI ATTAAT 1 cut(s) 312
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 4 cut(s) 167, 241, 299, 662
AsuC2I CCSGG 1 cut(s) 703
AsuHPI GGTGA 2 cut(s) 216, 611
AsuII TTCGAA 1 cut(s) 822
AsuNHI GCTAGC 1 cut(s) 262
AvaII GGWCC 2 cut(s) 167, 241
BalI TGGCCA 1 cut(s) 45
BanI GGYRCC 2 cut(s) 214, 890
BanII GRGCYC 2 cut(s) 385, 772
BarI GAAGNNNNNNTAC 2 cut(s) 1016, 1048
Bbv12I GWGCWC 1 cut(s) 772
BccI CCATC 5 cut(s) 476, 533, 593, 650, 983
BceAI ACGGC 2 cut(s) 405, 1105
BciT130I CCWGG 4 cut(s) 252, 303, 519, 666
BciVI GTATCC 1 cut(s) 1136
BcnI CCSGG 1 cut(s) 703
BcoDI GTCTC 3 cut(s) 199, 615, 937
BfaI CTAG 2 cut(s) 263, 1071
BfmI CTRYAG 1 cut(s) 1047
BfoI RGCGCY 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 778
BfuI GTATCC 1 cut(s) 1136
BmcAI AGTACT 1 cut(s) 964
Bme1390I CCNGG 5 cut(s) 252, 303, 519, 666, 703
Bme18I GGWCC 2 cut(s) 167, 241
BmgT120I GGNCC 4 cut(s) 167, 241, 299, 662
BmiI GGNNCC 3 cut(s) 216, 384, 892
BmrFI CCNGG 5 cut(s) 252, 303, 519, 666, 703
BmrI ACTGGG 1 cut(s) 374
BmsI GCATC 3 cut(s) 64, 195, 359
BmtI GCTAGC 1 cut(s) 266
BmuI ACTGGG 1 cut(s) 374
BpmI CTGGAG 2 cut(s) 501, 857
Bpu10I CCTNAGC 1 cut(s) 691
Bpu14I TTCGAA 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 703
Bsa29I ATCGAT 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 3 cut(s) 301, 664, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 671, 1010
Bse1I ACTGG 4 cut(s) 66, 369, 916, 1015
Bse3DI GCAATG 1 cut(s) 1092
BseBI CCWGG 4 cut(s) 252, 303, 519, 666
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 3 cut(s) 301, 664, 751
BseGI GGATG 3 cut(s) 79, 355, 374
BseLI CCNNNNNNNGG 3 cut(s) 365, 671, 1010
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 4 cut(s) 66, 369, 916, 1015
BshFI GGCC 4 cut(s) 45, 300, 663, 1114
BshNI GGYRCC 2 cut(s) 214, 890
BshVI ATCGAT 1 cut(s) 864
BsiHKAI GWGCWC 1 cut(s) 772
BsiSI CCGG 1 cut(s) 702
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 3 cut(s) 365, 671, 1010
BsmAI GTCTC 3 cut(s) 199, 615, 937
BsmFI GGGAC 1 cut(s) 513
BsmI GAATGC 2 cut(s) 65, 88
BsnI GGCC 4 cut(s) 45, 300, 663, 1114
Bso31I GGTCTC 1 cut(s) 615
Bsp119I TTCGAA 1 cut(s) 822
Bsp1286I GDGCHC 2 cut(s) 385, 772
Bsp143I GATC 4 cut(s) 247, 774, 865, 1040
BspANI GGCC 4 cut(s) 45, 300, 663, 1114
BspCNI CTCAG 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 864
BspLI GGNNCC 3 cut(s) 216, 384, 892
BspMI ACCTGC 1 cut(s) 778
BspOI GCTAGC 1 cut(s) 266
BspPI GGATC 2 cut(s) 242, 782
BspT104I TTCGAA 1 cut(s) 822
BspT107I GGYRCC 2 cut(s) 214, 890
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 1092
BsrI ACTGG 4 cut(s) 66, 369, 916, 1015
BssECI CCNNGG 3 cut(s) 301, 664, 751
BssMI GATC 4 cut(s) 247, 774, 865, 1040
BssNAI GTATAC 1 cut(s) 1033
BssT1I CCWWGG 1 cut(s) 751
Bst1107I GTATAC 1 cut(s) 1033
Bst2UI CCWGG 4 cut(s) 252, 303, 519, 666
Bst6I CTCTTC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 3 cut(s) 264, 298, 895
BstDEI CTNAG 3 cut(s) 97, 425, 691
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 3 cut(s) 79, 355, 374
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 4 cut(s) 250, 777, 868, 1043
BstMAI GTCTC 3 cut(s) 199, 615, 937
BstMBI GATC 4 cut(s) 247, 774, 865, 1040
BstMWI GCNNNNNNNGC 4 cut(s) 83, 389, 417, 687
BstNI CCWGG 4 cut(s) 252, 303, 519, 666
BstNSI RCATGY 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 5 cut(s) 250, 301, 517, 664, 701
BstSFI CTRYAG 1 cut(s) 1047
BstXI CCANNNNNNTGG 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 1033
Bsu15I ATCGAT 1 cut(s) 864
BsuI GTATCC 1 cut(s) 1136
BsuRI GGCC 4 cut(s) 45, 300, 663, 1114
BsuTUI ATCGAT 1 cut(s) 864
BtsCI GGATG 3 cut(s) 79, 355, 374
BtsIMutI CAGTG 4 cut(s) 43, 59, 534, 1008
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 3 cut(s) 264, 298, 895
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 4 cut(s) 167, 241, 299, 662
ClaI ATCGAT 1 cut(s) 864
Csp6I GTAC 2 cut(s) 306, 963
CviQI GTAC 2 cut(s) 306, 963
DdeI CTNAG 3 cut(s) 97, 425, 691
DpnI GATC 4 cut(s) 249, 776, 867, 1042
DpnII GATC 4 cut(s) 247, 774, 865, 1040
EaeI YGGCCR 2 cut(s) 43, 1112
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Ecl136II GAGCTC 1 cut(s) 770
Eco130I CCWWGG 1 cut(s) 751
Eco24I GRGCYC 2 cut(s) 385, 772
Eco31I GGTCTC 1 cut(s) 615
Eco32I GATATC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 167, 241
Eco47III AGCGCT 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 770
Eco57I CTGAAG 1 cut(s) 249
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 770
EcoO109I RGGNCCY 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 4 cut(s) 250, 301, 517, 664
EcoRV GATATC 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 751
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 2 cut(s) 385, 772
ErhI CCWWGG 1 cut(s) 751
FaqI GGGAC 1 cut(s) 513
FblI GTMKAC 1 cut(s) 1032
FokI GGATG 3 cut(s) 86, 342, 381
FriOI GRGCYC 2 cut(s) 385, 772
FspBI CTAG 2 cut(s) 263, 1071
GlaI GCGC 1 cut(s) 329
GsuI CTGGAG 2 cut(s) 501, 857
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 4 cut(s) 45, 300, 663, 1114
HapII CCGG 1 cut(s) 702
HhaI GCGC 1 cut(s) 330
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 7 cut(s) 285, 337, 486, 499, 544, 819, 839
HpaII CCGG 1 cut(s) 702
HphI GGTGA 2 cut(s) 216, 611
Hpy166II GTNNAC 2 cut(s) 919, 1033
Hpy188I TCNGA 5 cut(s) 100, 774, 818, 984, 1136
Hpy188III TCNNGA 3 cut(s) 490, 778, 836
Hpy8I GTNNAC 2 cut(s) 919, 1033
HpyAV CCTTC 5 cut(s) 126, 232, 391, 946, 1046
HpyCH4IV ACGT 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 389, 417, 687
HpyF3I CTNAG 3 cut(s) 97, 425, 691
HpySE526I ACGT 1 cut(s) 15
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 4 cut(s) 247, 774, 865, 1040
LmnI GCTCC 6 cut(s) 29, 388, 455, 520, 767, 1001
LweI GCATC 3 cut(s) 64, 195, 359
MaeI CTAG 2 cut(s) 263, 1071
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 3 cut(s) 222, 742, 1063
MalI GATC 4 cut(s) 249, 776, 867, 1042
MboI GATC 4 cut(s) 247, 774, 865, 1040
MboII GAAGA 2 cut(s) 330, 836
MfeI CAATTG 1 cut(s) 615
MhlI GDGCHC 2 cut(s) 385, 772
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 4 cut(s) 123, 190, 615, 985
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 2 cut(s) 279, 848
MmeI TCCRAC 2 cut(s) 410, 1033
MnlI CCTC 6 cut(s) 314, 466, 568, 758, 803, 892
Mox20I TGGCCA 1 cut(s) 45
Mph1103I ATGCAT 1 cut(s) 79
MscI TGGCCA 1 cut(s) 45
MseI TTAA 6 cut(s) 12, 279, 312, 467, 654, 846
MslI CAYNNNNRTG 1 cut(s) 924
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 1 cut(s) 702
MspR9I CCNGG 5 cut(s) 252, 303, 519, 666, 703
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 2 cut(s) 65, 88
MvaI CCWGG 4 cut(s) 252, 303, 519, 666
MwoI GCNNNNNNNGC 4 cut(s) 83, 389, 417, 687
NciI CCSGG 1 cut(s) 703
NdeII GATC 4 cut(s) 247, 774, 865, 1040
NheI GCTAGC 1 cut(s) 262
NlaIV GGNNCC 3 cut(s) 216, 384, 892
NmuCI GTSAC 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 924
PciI ACATGT 1 cut(s) 89
PctI GAATGC 2 cut(s) 65, 88
PfeI GAWTC 5 cut(s) 337, 486, 499, 544, 819
PfoI TCCNGGA 2 cut(s) 517, 701
PleI GAGTC 2 cut(s) 279, 847
PpsI GAGTC 2 cut(s) 279, 847
PpuMI RGGWCCY 1 cut(s) 241
PscI ACATGT 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 312
PsiI TTATAA 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 772
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 4 cut(s) 250, 301, 517, 664
PspEI GGTNACC 1 cut(s) 742
PspGI CCWGG 4 cut(s) 250, 301, 517, 664
PspN4I GGNNCC 3 cut(s) 216, 384, 892
PspPI GGNCC 4 cut(s) 167, 241, 299, 662
PspPPI RGGWCCY 1 cut(s) 241
PsrI GAACNNNNNNTAC 2 cut(s) 946, 978
PstNI CAGNNNCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 307, 964
RsaNI GTAC 2 cut(s) 306, 963
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 1 cut(s) 772
SaqAI TTAA 6 cut(s) 12, 279, 312, 467, 654, 846
Sau3AI GATC 4 cut(s) 247, 774, 865, 1040
Sau96I GGNCC 4 cut(s) 167, 241, 299, 662
ScaI AGTACT 1 cut(s) 964
SchI GAGTC 2 cut(s) 279, 848
ScrFI CCNGG 5 cut(s) 252, 303, 519, 666, 703
SduI GDGCHC 2 cut(s) 385, 772
SfaNI GCATC 3 cut(s) 64, 195, 359
SfcI CTRYAG 1 cut(s) 1047
SfuI TTCGAA 1 cut(s) 822
SinI GGWCC 2 cut(s) 167, 241
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 4 cut(s) 123, 190, 615, 985
SspI AATATT 2 cut(s) 637, 1017
SspMI CTAG 2 cut(s) 263, 1071
SstI GAGCTC 1 cut(s) 772
StyD4I CCNGG 5 cut(s) 250, 301, 517, 664, 701
StyI CCWWGG 1 cut(s) 751
TaiI ACGT 1 cut(s) 18
TaqI TCGA 5 cut(s) 808, 822, 864, 959, 1039
TasI AATT 4 cut(s) 123, 190, 615, 985
TatI WGTACW 1 cut(s) 962
TfiI GAWTC 5 cut(s) 337, 486, 499, 544, 819
Tru1I TTAA 6 cut(s) 12, 279, 312, 467, 654, 846
Tru9I TTAA 6 cut(s) 12, 279, 312, 467, 654, 846
TscAI CASTG 4 cut(s) 43, 66, 541, 1015
TseFI GTSAC 1 cut(s) 222
Tsp45I GTSAC 1 cut(s) 222
TspDTI ATGAA 3 cut(s) 260, 704, 719
TspGWI ACGGA 2 cut(s) 780, 988
TspRI CASTG 4 cut(s) 43, 66, 541, 1015
VpaK11BI GGWCC 2 cut(s) 167, 241
VspI ATTAAT 1 cut(s) 312
XceI RCATGY 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 2 cut(s) 263, 1071
ZrmI AGTACT 1 cut(s) 964
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.