Rroxscaffold_2G00152660

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014535
Physical Location & Seq
Reverse (-)
89546380 .. 89551834
5455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152660.1

Sequence Viewer

Length: 2673 bp
ATGACAGCCTCCAGCGGGGGTGTGGCAGTTCCGGTGAATGTTGACAAAAGGTACATAATGCAGCGAGGACGTTCCGGGTTCTATACCTATGCCATCTTTGAGCGTCTTGAAGGGTGGCCGGGAGGGGACATGGAGACACTTAGAGTGGCTTACAAGCTTCATCAAGCCAAGTTTCGATATATGGCGCTATCGGACACTAGGCAAAGGTTCATGCCAACAGCCGATGACCGAGCACACGGTAGGCCTCTTGCATTCAAAGAAGCTGTTCTACTGAACAATAAGACAAGCAGTCCTGAATTTGAAGGGGAGGTGGATGATAAGTATCAGTACTCGGCCGAGGACAAAGATATCAAGGTGCATGGTTGGATTTCTATTGATGACCAATCACCACCGGTGGGATTTTGGATGATCACACCTAGTGATGAGTTTCGTATAGGTGGTCCTTTCAAGCAGGACCTCACCTCTCACGTTGGACCAACTTCCCTAACTGTGTTTTCTTCTCATCATTATGTTGCTCGAAACTCAGCTAATAGTCCTTCAACGATCCGTACACTATGGAACAATGCCAAAGATCAAATGCTTGAAGAAGTCAAAAGTTGGCCATACAATTTCATTAGTTCTCACGACTTTCCGTCTTCAGATCAACGTGGATCAGTTTCGGGCCAGTTACTAGTACATGATCGATACATTGAAAGTCTTCCTTGGACAAGTTTGGCTTACGTAGGATTGGCTGCACCCGGAGAAGTTGGATCATGGCAAAGGGAAAGCAAGGGATACCAATTTTGGACTCAAACCGATGAAAAAGGCTATTTCCTCATTGAAAATATTAGACCCGGTAACTATAATTTGTATGCATCTGTTCCAGGGATAGTTGGAGACTACAAATATGAGGTTGACATTACAATTGAACCAGGAAGTAAAATCCAATTGGCCAGTATTACTTACAAACCTCCGAGAGCCGGTCCTACATTGTGGGAAATTGGCATTCCTGATCGCACAGCTGCGGAGTTCTACATACCGGATCCATATCCTACTCTCATGAACAAGTTGTACAAGGGGAGTCGGTTGGACAAGTTTAGGCAATACGGGTATGTAGGAACTACATGGCGGATACAGTTTGAACTCAACAATGTGGTCAATCCCGGAAACTATACACTTCAACTGGCCTTGGCATCAGCCACTTATGCAGAATTGCAAGTTCGGTTTAACAATTTGAATGCCAAACCACCTCTTTTTTCGACAGGGCTAATAGGTGACGACAATGCTATTGCAAGACATGGAATTCATGGCTTATACTGGTTATGGAGTATTGATGTACCGAGCACTCTACTACGCGAAGGAAGCAACACCATCTATCTTACTCAGTCCAGAGATGGCAATTCACCTTTCCAAGGAGTCATTATTTGTTTCCTAAAAGAACTTATGAGAAATCATCTAGCAGCTTATGTGAGTTCTTTGATTTCTGATATATATATAACTGTCAAGCACTATTTGGAATTTGAAGAGGATAACATAAAGTTCAGCTTCCTTTTGGTGGTGTCAATTCCACAAATGATGGGGAGCTATCTTTTTCTAGTTTTTCTTGGGACAGTTTCTGTGTTTACTGCATGTTCAGAACATGAACATGCAAACGCTACAATGGCTACTTTTAATGTCTTGGACTATGGAGCTGTCAGTGGTGGCCACATAGACAATGCACTGGCATTCTTGGATGCATGGAATGCAACATGTAGCTCAGAAGCTGAAAACTCGACTCTAATCATTCCCAGAAGGAGATTTCTTGTAAATCCAACCACTTTCAATGGTCCATGCAAAGCCCAAAGCATCAATTTTGTGATATTAGGGAGACTTTTGGCACCAAAGTCACCTGAAGCATGGAAGGACCTTGATCCAGGTCAATGGCTAGCATTTCATGGTGTTAATGGACTCAAGGTTGCTGGCCCTGGTACTATTAATGGAAGAGGAAAGCGCTGGTGGAATCAATCTTGCAGATATCATCCAAAACTGGAGGGATGCACTTCACGGGCTCCAACGGCTTTGAAGGTTCTTTCATGCAAGAAAAGCTGTCTTAGAGATATTCACTTTATAAACAGCTCCCAAGCCCATGTCTTAATAGAGGGGTGTGATGGAATCAAGATTGAGAATCTTATGATTGAAGCTCCAGGAAATAGTCCCAACACTGATGGGATTCACATTCAGTCCTCTCAAAATGTCATTATCAACAACACCATCATAGGCTCTGGTGATGATTGTATATCAATTGGAGACCATATATCCAATATTGCCATCTCATATATTAAATGTGGCCCTGGTCATGGAATAAGCATTGGAAGCTTAGGGAAATCCGGGAATGTCGTTCATGTAGAGAACATTCATGTCAGTAAGGTTACCCTCCAAGGAACTACGAACGGAGCTCGGATCAAGACATGGCAGGTTGGCAGGGGTTCTGTTCGAGGGGTCAGATTCGAAGATTTCTTTCTGGAGTCTGTTAAGAACCCCATAATCATCGATCAAAACTACTGCGAAGTTAGGGGTGCCTGCGAGGAACTGCCGACTGGTGTTCACATAAGTGATGTCTCCTTCAAAAAACTTCTATGGAACATCGAGTACCAATATCGCCATCAATCTGAATTGCAGTCGCTCCATATGCCTACGGCATTGCCTTTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

890

Amino Acids

99.31

Weight (kDa)

6.54

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 3 - 104 6.6e-37 Rhamnogalacturonate lyase family
fn3_3 PF14686 239 - 310 3.5e-24 Polysaccharide lyase family 4, domain II
CBM-like PF14683 323 - 468 7.5e-33 Polysaccharide lyase family 4, domain III
Glyco_hydro_28 PF00295 580 - 863 2.1e-77 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80140
fragaria_vesca FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310 FvH4_1g03310
prunus_persica Prupe.7G242600_v2.0.a1
pyrus_communis pycom15g15490
rosa_chinensis RchiOBHm_Chr2g0088471
rosa_laevigata RLG00000015957
rosa_roxburghii Rroxscaffold_2G00152660
rosa_rugosa Rorug01G0484300
rosa_samantha Rh2AG039400 Rh2BG038200 Rh2CG039500 Rh2DG038900
rosa_wichuraiana Rw2G003090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2087
Acc36I ACCTGC 1 cut(s) 2422
AccB1I GGYRCC 2 cut(s) 1855, 2534
AccII CGCG 1 cut(s) 1335
AciI CCGC 3 cut(s) 15, 1004, 1108
AclWI GGATC 7 cut(s) 538, 658, 757, 1016, 1029, 1883, 2426
AcoI YGGCCR 5 cut(s) 116, 333, 599, 930, 1681
AcsI RAATTY 3 cut(s) 296, 1281, 1496
AcuI CTGAAG 2 cut(s) 621, 1890
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 8 cut(s) 53, 329, 550, 675, 1052, 1317, 1948, 2609
AfeI AGCGCT 1 cut(s) 1970
AfiI CCNNNNNNNGG 6 cut(s) 15, 395, 959, 1391, 1534, 2315
AflIII ACRYGT 1 cut(s) 1727
AgeI ACCGGT 1 cut(s) 391
AhdI GACNNNNNGTC 2 cut(s) 288, 631
AhlI ACTAGT 1 cut(s) 670
AjnI CCWGG 6 cut(s) 862, 910, 1891, 1942, 2161, 2308
AleI CACNNNNGTG 1 cut(s) 2568
Alw21I GWGCWC 3 cut(s) 235, 1325, 2416
Alw26I GTCTC 5 cut(s) 128, 870, 1840, 2259, 2581
AlwI GGATC 7 cut(s) 538, 658, 757, 1016, 1029, 1883, 2426
AlwNI CAGNNNCTG 2 cut(s) 1595, 1742
Aor51HI AGCGCT 1 cut(s) 1970
ApeKI GCWGC 4 cut(s) 61, 731, 1001, 1439
ApoI RAATTY 3 cut(s) 296, 1281, 1496
AseI ATTAAT 1 cut(s) 1953
AsiGI ACCGGT 1 cut(s) 391
Asp700I GAANNNNTTC 2 cut(s) 264, 696
AspLEI GCGC 2 cut(s) 187, 1971
AspS9I GGNCC 9 cut(s) 440, 454, 473, 661, 962, 1805, 1882, 1940, 2306
AsuC2I CCSGG 6 cut(s) 76, 120, 738, 834, 1143, 2347
AsuHPI GGTGA 7 cut(s) 46, 378, 451, 1265, 1374, 1857, 2255
AsuII TTCGAA 1 cut(s) 2466
AsuNHI GCTAGC 1 cut(s) 1903
AvaII GGWCC 6 cut(s) 440, 454, 473, 962, 1805, 1882
BalI TGGCCA 3 cut(s) 601, 932, 1683
BamHI GGATCC 1 cut(s) 1021
BanI GGYRCC 2 cut(s) 1855, 2534
BanII GRGCYC 2 cut(s) 2029, 2416
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BbsI GAAGAC 2 cut(s) 627, 689
Bbv12I GWGCWC 3 cut(s) 235, 1325, 2416
BbvI GCAGC 4 cut(s) 73, 718, 988, 1451
BccI CCATC 9 cut(s) 101, 1358, 1367, 1549, 2120, 2177, 2237, 2294, 2628
BceAI ACGGC 2 cut(s) 2049, 2670
BciT130I CCWGG 6 cut(s) 864, 912, 1893, 1944, 2163, 2310
BciVI GTATCC 2 cut(s) 767, 1104
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 6 cut(s) 76, 120, 738, 834, 1143, 2347
BcoDI GTCTC 5 cut(s) 128, 870, 1840, 2259, 2581
BcuI ACTAGT 1 cut(s) 670
BfaI CTAG 6 cut(s) 198, 417, 671, 1436, 1574, 1904
BfoI RGCGCY 2 cut(s) 188, 1972
BfuAI ACCTGC 1 cut(s) 2422
BfuI GTATCC 2 cut(s) 767, 1104
BisI GCNGC 4 cut(s) 62, 732, 1002, 1440
BlsI GCNGC 4 cut(s) 63, 733, 1003, 1441
BmcAI AGTACT 1 cut(s) 329
Bme18I GGWCC 6 cut(s) 440, 454, 473, 962, 1805, 1882
BmeRI GACNNNNNGTC 2 cut(s) 288, 631
BmgT120I GGNCC 9 cut(s) 440, 454, 473, 661, 962, 1805, 1882, 1940, 2306
BmiI GGNNCC 4 cut(s) 1023, 1857, 2028, 2536
BmsI GCATC 5 cut(s) 863, 1181, 1702, 1833, 2003
BmtI GCTAGC 1 cut(s) 1907
BpiI GAAGAC 2 cut(s) 627, 689
BpmI CTGGAG 3 cut(s) 2027, 2145, 2501
Bpu10I CCTNAGC 1 cut(s) 2335
Bpu14I TTCGAA 1 cut(s) 2466
BpuEI CTTGAG 1 cut(s) 1913
BpuMI CCSGG 6 cut(s) 76, 120, 738, 834, 1143, 2347
Bsa29I ATCGAT 2 cut(s) 682, 2508
BsaAI YACGTR 1 cut(s) 721
BsaBI GATNNNNATC 2 cut(s) 321, 1026
BsaI GGTCTC 1 cut(s) 2259
BsaJI CCNNGG 8 cut(s) 336, 701, 863, 1167, 1390, 1942, 2308, 2395
BsaWI WCCGGW 3 cut(s) 31, 391, 1018
Bsc4I CCNNNNNNNGG 6 cut(s) 15, 395, 959, 1391, 1534, 2315
Bse118I RCCGGY 2 cut(s) 391, 959
Bse1I ACTGG 7 cut(s) 664, 933, 1167, 1301, 1704, 2010, 2560
Bse3DI GCAATG 1 cut(s) 2657
Bse8I GATNNNNATC 2 cut(s) 321, 1026
BseBI CCWGG 6 cut(s) 864, 912, 1893, 1944, 2163, 2310
BseCI ATCGAT 2 cut(s) 682, 2508
BseDI CCNNGG 8 cut(s) 336, 701, 863, 1167, 1390, 1942, 2308, 2395
BseGI GGATG 5 cut(s) 319, 411, 1717, 1996, 2018
BseJI GATNNNNATC 2 cut(s) 321, 1026
BseLI CCNNNNNNNGG 6 cut(s) 15, 395, 959, 1391, 1534, 2315
BseMI GCAATG 1 cut(s) 2657
BseMII CTCAG 3 cut(s) 537, 1376, 1749
BseNI ACTGG 7 cut(s) 664, 933, 1167, 1301, 1704, 2010, 2560
BseX3I CGGCCG 1 cut(s) 333
BseXI GCAGC 4 cut(s) 73, 718, 988, 1451
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 1335
Bsh1285I CGRYCG 1 cut(s) 336
BshNI GGYRCC 2 cut(s) 1855, 2534
BshTI ACCGGT 1 cut(s) 391
BshVI ATCGAT 2 cut(s) 682, 2508
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 3 cut(s) 235, 1325, 2416
BslFI GGGAC 3 cut(s) 140, 1600, 2157
BslI CCNNNNNNNGG 6 cut(s) 15, 395, 959, 1391, 1534, 2315
BsmAI GTCTC 5 cut(s) 128, 870, 1840, 2259, 2581
BsmFI GGGAC 3 cut(s) 140, 1600, 2157
BsmI GAATGC 5 cut(s) 251, 984, 1222, 1703, 1726
Bso31I GGTCTC 1 cut(s) 2259
Bsp119I TTCGAA 1 cut(s) 2466
Bsp1286I GDGCHC 4 cut(s) 235, 1325, 2029, 2416
Bsp1407I TGTACA 1 cut(s) 1050
BspACI CCGC 3 cut(s) 15, 1004, 1108
BspCNI CTCAG 3 cut(s) 536, 1375, 1748
BspDI ATCGAT 2 cut(s) 682, 2508
BspFNI CGCG 1 cut(s) 1335
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 4 cut(s) 1023, 1857, 2028, 2536
BspMI ACCTGC 1 cut(s) 2422
BspOI GCTAGC 1 cut(s) 1907
BspPI GGATC 7 cut(s) 538, 658, 757, 1016, 1029, 1883, 2426
BspT104I TTCGAA 1 cut(s) 2466
BspT107I GGYRCC 2 cut(s) 1855, 2534
BspTNI GGTCTC 1 cut(s) 2259
BsrDI GCAATG 1 cut(s) 2657
BsrFI RCCGGY 2 cut(s) 391, 959
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 7 cut(s) 664, 933, 1167, 1301, 1704, 2010, 2560
BssAI RCCGGY 2 cut(s) 391, 959
BssECI CCNNGG 8 cut(s) 336, 701, 863, 1167, 1390, 1942, 2308, 2395
BssT1I CCWWGG 4 cut(s) 701, 1167, 1390, 2395
Bst2UI CCWGG 6 cut(s) 864, 912, 1893, 1944, 2163, 2310
Bst4CI ACNGT 5 cut(s) 239, 490, 1116, 1480, 1591
Bst6I CTCTTC 2 cut(s) 1497, 1954
BstAPI GCANNNNNTGC 1 cut(s) 1721
BstAUI TGTACA 1 cut(s) 1050
BstBAI YACGTR 1 cut(s) 721
BstBI TTCGAA 1 cut(s) 2466
BstC8I GCNNGC 3 cut(s) 1905, 1939, 2539
BstDEI CTNAG 6 cut(s) 140, 523, 1362, 1735, 2069, 2335
BstEII GGTNACC 1 cut(s) 2386
BstENI CCTNNNNNAGG 1 cut(s) 1389
BstF5I GGATG 5 cut(s) 319, 411, 1717, 1996, 2018
BstFNI CGCG 1 cut(s) 1335
BstH2I RGCGCY 2 cut(s) 188, 1972
BstHHI GCGC 2 cut(s) 187, 1971
BstMAI GTCTC 5 cut(s) 128, 870, 1840, 2259, 2581
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 8 cut(s) 1184, 1341, 1640, 1721, 2033, 2061, 2331, 2647
BstNI CCWGG 6 cut(s) 864, 912, 1893, 1944, 2163, 2310
BstNSI RCATGY 3 cut(s) 1611, 1628, 1731
BstPI GGTNACC 1 cut(s) 2386
BstSNI TACGTA 1 cut(s) 721
BstUI CGCG 1 cut(s) 1335
BstV1I GCAGC 4 cut(s) 73, 718, 988, 1451
BstV2I GAAGAC 2 cut(s) 627, 689
BstX2I RGATCY 1 cut(s) 1021
BstXI CCANNNNNNTGG 1 cut(s) 1899
BstYI RGATCY 1 cut(s) 1021
BstZI CGGCCG 1 cut(s) 333
Bsu15I ATCGAT 2 cut(s) 682, 2508
BsuI GTATCC 2 cut(s) 767, 1104
BsuTUI ATCGAT 2 cut(s) 682, 2508
BtsCI GGATG 5 cut(s) 319, 411, 1717, 1996, 2018
BtsIMutI CAGTG 3 cut(s) 1681, 1697, 2178
BveI ACCTGC 1 cut(s) 2422
Cac8I GCNNGC 3 cut(s) 1905, 1939, 2539
CaiI CAGNNNCTG 2 cut(s) 1595, 1742
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 2 cut(s) 187, 1971
Cfr10I RCCGGY 2 cut(s) 391, 959
Cfr13I GGNCC 9 cut(s) 440, 454, 473, 661, 962, 1805, 1882, 1940, 2306
ClaI ATCGAT 2 cut(s) 682, 2508
CseI GACGC 1 cut(s) 92
Csp6I GTAC 8 cut(s) 52, 328, 549, 674, 1051, 1316, 1947, 2608
CspAI ACCGGT 1 cut(s) 391
CviQI GTAC 8 cut(s) 52, 328, 549, 674, 1051, 1316, 1947, 2608
DdeI CTNAG 6 cut(s) 140, 523, 1362, 1735, 2069, 2335
DraIII CACNNNGTG 1 cut(s) 419
DriI GACNNNNNGTC 2 cut(s) 288, 631
EaeI YGGCCR 5 cut(s) 116, 333, 599, 930, 1681
EagI CGGCCG 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 1497, 1954
Eam1105I GACNNNNNGTC 2 cut(s) 288, 631
EarI CTCTTC 2 cut(s) 1497, 1954
EciI GGCGGA 1 cut(s) 1123
Ecl136II GAGCTC 1 cut(s) 2414
EclXI CGGCCG 1 cut(s) 333
Eco105I TACGTA 1 cut(s) 721
Eco130I CCWWGG 4 cut(s) 701, 1167, 1390, 2395
Eco147I AGGCCT 1 cut(s) 244
Eco24I GRGCYC 2 cut(s) 2029, 2416
Eco31I GGTCTC 1 cut(s) 2259
Eco32I GATATC 2 cut(s) 349, 1994
Eco47I GGWCC 6 cut(s) 440, 454, 473, 962, 1805, 1882
Eco47III AGCGCT 1 cut(s) 1970
Eco52I CGGCCG 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 2414
Eco57I CTGAAG 2 cut(s) 621, 1890
Eco91I GGTNACC 1 cut(s) 2386
EcoICRI GAGCTC 1 cut(s) 2414
EcoNI CCTNNNNNAGG 1 cut(s) 1389
EcoO109I RGGNCCY 2 cut(s) 454, 1882
EcoO65I GGTNACC 1 cut(s) 2386
EcoRI GAATTC 1 cut(s) 1281
EcoRII CCWGG 6 cut(s) 862, 910, 1891, 1942, 2161, 2308
EcoRV GATATC 2 cut(s) 349, 1994
EcoT14I CCWWGG 4 cut(s) 701, 1167, 1390, 2395
EcoT22I ATGCAT 2 cut(s) 856, 1717
EcoT38I GRGCYC 2 cut(s) 2029, 2416
ErhI CCWWGG 4 cut(s) 701, 1167, 1390, 2395
FalI AAGNNNNNCTT 6 cut(s) 685, 717, 700, 732, 1508, 1540
FaqI GGGAC 3 cut(s) 140, 1600, 2157
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 1 cut(s) 2646
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 4 cut(s) 62, 732, 1002, 1440
FokI GGATG 5 cut(s) 326, 418, 1724, 1983, 2025
FriOI GRGCYC 2 cut(s) 2029, 2416
Fsp4HI GCNGC 4 cut(s) 62, 732, 1002, 1440
FspBI CTAG 6 cut(s) 198, 417, 671, 1436, 1574, 1904
GlaI GCGC 2 cut(s) 186, 1970
GluI GCNGC 4 cut(s) 62, 732, 1002, 1440
GsuI CTGGAG 3 cut(s) 2027, 2145, 2501
HaeII RGCGCY 2 cut(s) 188, 1972
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 187, 1971
Hin6I GCGC 2 cut(s) 185, 1969
HinP1I GCGC 2 cut(s) 185, 1969
HincII GTYRAC 2 cut(s) 43, 895
HindII GTYRAC 2 cut(s) 43, 895
HindIII AAGCTT 2 cut(s) 155, 2332
HphI GGTGA 7 cut(s) 46, 378, 451, 1265, 1374, 1857, 2255
Hpy166II GTNNAC 5 cut(s) 43, 551, 895, 1602, 2563
Hpy188I TCNGA 9 cut(s) 193, 640, 954, 1465, 1615, 1738, 2418, 2462, 2629
Hpy188III TCNNGA 9 cut(s) 107, 293, 623, 989, 1039, 1368, 2134, 2422, 2480
Hpy8I GTNNAC 5 cut(s) 43, 551, 895, 1602, 2563
HpyAV CCTTC 8 cut(s) 104, 296, 546, 1331, 1764, 1873, 2035, 2590
HpyCH4III ACNGT 5 cut(s) 239, 490, 1116, 1480, 1591
HpyCH4IV ACGT 4 cut(s) 70, 468, 646, 720
HpyF10VI GCNNNNNNNGC 8 cut(s) 1184, 1341, 1640, 1721, 2033, 2061, 2331, 2647
HpyF3I CTNAG 6 cut(s) 140, 523, 1362, 1735, 2069, 2335
HpySE526I ACGT 4 cut(s) 70, 468, 646, 720
HspAI GCGC 2 cut(s) 185, 1969
Ksp22I TGATCA 1 cut(s) 408
LmnI GCTCC 7 cut(s) 1560, 1667, 2032, 2099, 2164, 2411, 2646
Lsp1109I GCAGC 4 cut(s) 73, 718, 988, 1451
LweI GCATC 5 cut(s) 863, 1181, 1702, 1833, 2003
MaeI CTAG 6 cut(s) 198, 417, 671, 1436, 1574, 1904
MaeII ACGT 4 cut(s) 70, 468, 646, 720
MaeIII GTNAC 5 cut(s) 666, 836, 1253, 1863, 2386
MboII GAAGA 7 cut(s) 489, 596, 627, 689, 1514, 1971, 2480
MfeI CAATTG 3 cut(s) 903, 926, 2259
MflI RGATCY 1 cut(s) 1021
MhlI GDGCHC 4 cut(s) 235, 1325, 2029, 2416
MlsI TGGCCA 3 cut(s) 601, 932, 1683
MluNI TGGCCA 3 cut(s) 601, 932, 1683
MlyI GAGTC 6 cut(s) 781, 1069, 1404, 1747, 1920, 2492
MmeI TCCRAC 7 cut(s) 344, 451, 727, 853, 1047, 1814, 2054
Mox20I TGGCCA 3 cut(s) 601, 932, 1683
Mph1103I ATGCAT 2 cut(s) 856, 1717
MroXI GAANNNNTTC 2 cut(s) 264, 696
MscI TGGCCA 3 cut(s) 601, 932, 1683
MseI TTAA 7 cut(s) 1206, 1650, 1920, 1953, 2111, 2298, 2490
MslI CAYNNNNRTG 3 cut(s) 507, 1623, 2568
Msp20I TGGCCA 3 cut(s) 601, 932, 1683
MspA1I CMGCKG 2 cut(s) 15, 1001
MunI CAATTG 3 cut(s) 903, 926, 2259
Mva1269I GAATGC 5 cut(s) 251, 984, 1222, 1703, 1726
MvaI CCWGG 6 cut(s) 864, 912, 1893, 1944, 2163, 2310
MvnI CGCG 1 cut(s) 1335
MwoI GCNNNNNNNGC 8 cut(s) 1184, 1341, 1640, 1721, 2033, 2061, 2331, 2647
NciI CCSGG 6 cut(s) 76, 120, 738, 834, 1143, 2347
NdeI CATATG 1 cut(s) 2646
NheI GCTAGC 1 cut(s) 1903
NlaIV GGNNCC 4 cut(s) 1023, 1857, 2028, 2536
NmeAIII GCCGAG 2 cut(s) 311, 361
NmuCI GTSAC 2 cut(s) 1253, 1863
NsiI ATGCAT 2 cut(s) 856, 1717
NspI RCATGY 3 cut(s) 1611, 1628, 1731
NspV TTCGAA 1 cut(s) 2466
OliI CACNNNNGTG 1 cut(s) 2568
PagI TCATGA 1 cut(s) 1038
PceI AGGCCT 1 cut(s) 244
PciI ACATGT 1 cut(s) 1727
PctI GAATGC 5 cut(s) 251, 984, 1222, 1703, 1726
PdmI GAANNNNTTC 2 cut(s) 264, 696
PfeI GAWTC 5 cut(s) 1978, 2130, 2143, 2188, 2463
PfoI TCCNGGA 3 cut(s) 1141, 2161, 2345
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 4 cut(s) 63, 733, 1003, 1441
PleI GAGTC 6 cut(s) 781, 1068, 1403, 1747, 1920, 2491
PpsI GAGTC 6 cut(s) 781, 1068, 1403, 1747, 1920, 2491
Ppu21I YACGTR 1 cut(s) 721
PpuMI RGGWCCY 2 cut(s) 454, 1882
PscI ACATGT 1 cut(s) 1727
PshBI ATTAAT 1 cut(s) 1953
PsiI TTATAA 1 cut(s) 2087
Psp124BI GAGCTC 1 cut(s) 2416
Psp5II RGGWCCY 2 cut(s) 454, 1882
Psp6I CCWGG 6 cut(s) 862, 910, 1891, 1942, 2161, 2308
PspEI GGTNACC 1 cut(s) 2386
PspGI CCWGG 6 cut(s) 862, 910, 1891, 1942, 2161, 2308
PspN4I GGNNCC 4 cut(s) 1023, 1857, 2028, 2536
PspPI GGNCC 9 cut(s) 440, 454, 473, 661, 962, 1805, 1882, 1940, 2306
PspPPI RGGWCCY 2 cut(s) 454, 1882
PsrI GAACNNNNNNTAC 4 cut(s) 1034, 1066, 2591, 2623
PstNI CAGNNNCTG 2 cut(s) 1595, 1742
PsuI RGATCY 1 cut(s) 1021
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 8 cut(s) 53, 329, 550, 675, 1052, 1317, 1948, 2609
RsaNI GTAC 8 cut(s) 52, 328, 549, 674, 1051, 1316, 1947, 2608
RseI CAYNNNNRTG 3 cut(s) 507, 1623, 2568
SacI GAGCTC 1 cut(s) 2416
SaqAI TTAA 7 cut(s) 1206, 1650, 1920, 1953, 2111, 2298, 2490
SatI GCNGC 4 cut(s) 62, 732, 1002, 1440
Sau96I GGNCC 9 cut(s) 440, 454, 473, 661, 962, 1805, 1882, 1940, 2306
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 6 cut(s) 781, 1069, 1404, 1747, 1920, 2492
SduI GDGCHC 4 cut(s) 235, 1325, 2029, 2416
SfaNI GCATC 5 cut(s) 863, 1181, 1702, 1833, 2003
SfuI TTCGAA 1 cut(s) 2466
SgrAI CRCCGGYG 1 cut(s) 391
SinI GGWCC 6 cut(s) 440, 454, 473, 962, 1805, 1882
SmiMI CAYNNNNRTG 3 cut(s) 507, 1623, 2568
SmlI CTYRAG 1 cut(s) 1928
SmoI CTYRAG 1 cut(s) 1928
SnaBI TACGTA 1 cut(s) 721
SpeI ACTAGT 1 cut(s) 670
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 3 cut(s) 15, 1004, 1108
SspI AATATT 2 cut(s) 826, 2281
SspMI CTAG 6 cut(s) 198, 417, 671, 1436, 1574, 1904
SstI GAGCTC 1 cut(s) 2416
StuI AGGCCT 1 cut(s) 244
StyI CCWWGG 4 cut(s) 701, 1167, 1390, 2395
TaaI ACNGT 5 cut(s) 239, 490, 1116, 1480, 1591
TaiI ACGT 4 cut(s) 73, 471, 649, 723
TaqI TCGA 9 cut(s) 175, 517, 682, 1238, 1751, 2452, 2466, 2508, 2604
TaqII GACCGA 1 cut(s) 243
TatI WGTACW 3 cut(s) 327, 673, 1050
TfiI GAWTC 5 cut(s) 1978, 2130, 2143, 2188, 2463
Tru1I TTAA 7 cut(s) 1206, 1650, 1920, 1953, 2111, 2298, 2490
Tru9I TTAA 7 cut(s) 1206, 1650, 1920, 1953, 2111, 2298, 2490
TscAI CASTG 3 cut(s) 1681, 1704, 2185
TseFI GTSAC 2 cut(s) 1253, 1863
TseI GCWGC 4 cut(s) 61, 731, 1001, 1439
Tsp45I GTSAC 2 cut(s) 1253, 1863
TspGWI ACGGA 3 cut(s) 536, 621, 2424
TspRI CASTG 3 cut(s) 1681, 1704, 2185
VpaK11BI GGWCC 6 cut(s) 440, 454, 473, 962, 1805, 1882
VspI ATTAAT 1 cut(s) 1953
XagI CCTNNNNNAGG 1 cut(s) 1389
XapI RAATTY 3 cut(s) 296, 1281, 1496
XceI RCATGY 3 cut(s) 1611, 1628, 1731
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 2 cut(s) 264, 696
XspI CTAG 6 cut(s) 198, 417, 671, 1436, 1574, 1904
ZrmI AGTACT 1 cut(s) 329
Zsp2I ATGCAT 2 cut(s) 856, 1717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.