pycom15g20030

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007281
Physical Location & Seq
Reverse (-)
14316300 .. 14327258
10959 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2898 bp
ATGGAGAAATTTGCAGAAAGATATTGTGCAGATAATCCAGGTCTTTTCAAAAATGCAGACACTGCATACATTTTAGCATACGCGGTTATAATATTGAATACTGATGCGCATAATCCAATGGTGTGGCCAAAAATGTCCAAGTCAGATTTTATACTCATGAATGCTGCGGATGATGCAGAAGAGTGTGCTCCAACTGAACTTCTGGAAGAAATCTATTTTTCAATTGTTAAAGAAGAGATAAAGATGAAAAATGAGACTGCTGGTCTTGAGAAAAGTGGAAAATATAAGCGAGAAGGTGAAGAGAGAGGCCGCCTTGTCAGTATCCTTAATCTGGCTCTTCCCACAAGTGCGCTGTCCGTTGATACTAAGTCTGAAAGTGAAGCCATCATTAAGAAAACACAAGCTATATTTCGGAACCAAGGATCAAAAAGAGGGGTCTTCTATACTGCACAGCAGTTAGAGCTAGTAAGGCCTATGGTTGAAGCTGTAGGTTGGCCTTTGCTCGCTACTTTTTCTGTTACAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGTATGGAGGGATTGAAAGCTGGAATACACATTACACATGTTCTCGGAATGAATACCATGCGATATGCCTTTTTAACATCTCTGGTCAGATTTACTTTCTTGCATGCTCCGAAGGAAATGCGTAGTAAAAATGTGGAAGCACTGCGAACTCTACTATCTCTTTGTGACTCAGGGACTGGCTCCCTTCGAGATACATGGAATGCAGTTTTAGAATGTGTTTCTCGGCTTGAGTTCGTTACTTCAAATCCCTCTATAGCAGCAACAGTCATGCAAGGATCGAACCAAATCTCCAAAGATGCTCTTCTTCAATCTTTAAGGGAGTTGGCTGGAAAACCTTCTGAACAAGTATTTGTGAATAGCGTTCAGCTTCCTAGTGATTCTGTAGTGGAGTTCTTCACTGCACTCTGTGGTGTATCAGCTGAAGAACTAAAACAAACTCCAGCTCGTGTGTTTAGCTTGCAAAAACTTGTTGAGATCAGCTACTACAACATGGCTCGTATACGCATGGTCTGGGCTAGAATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCATCATGATGAAAAAATTGCTATGTATGCAATAGATTCCCTGAGGCAGCTTGGTATGAAGTATCTGGAGCGGGCGGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAACCTTTTGTTGTTCTTATGAGCAATAGTCGAAGTGAAACCATAAGGAGCTTAATAGTTGACTGCATCGTTCAAATGATAAAATCCAAAATAGGGAGCATCAAGTCTGGATGGCGTAACGTTTTCATGATTTTTACAGCTGCTGCAGATGATGAATTGGAATCAATTGTTGAGAGTGCATTTGAAAATGTAGAACAGGTTATCTTGGAACACTTCGATCGAGTAGTTGGATATTGTTTCATGGACTGTGTCAACTGTCTTATCAGGTTTGCTAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTATTGCGCATATGTGAGGATCGTCTGGCAGAGGGTCTTATACCTGGTGGTGCTTTGAGGCCTATTGACGTAAATGTGGATACAACCTTTGATGTGACTGAACATTACTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCAGATCCAAGACCAGAGGTCAGAAGCTGTGCGCTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTAGCAAATTCTCATCAGCATTTTGGGAGAGCATTTTTCATCGGGTCTTGTTTCCAATATTCGATCATGTGAGACATGTTGGAAAGGAAAGCTCGGTTTCCTCTGATGATGTATGGTTCCGTGAAACAAGCATTCACTCGCTGCAGTTGCTTTGCAACCTTTTCAACACGTTTTATAAGGAAGTATGTTTCATGTTGCCACCCCTACTCAGTCTACTTTTGGATTGTGCCAAAAAGACTGATCAAGCAGTTGTTTCATTATCTCTGGGTGCACTTGTGCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGCGACTGGGATACATTGTTGAAAAGCATAAGAGATGCTTTGTACACAACGCAACCACTTGAACTGCTAAACGCTTTGGGATTTGAGAATCTGAAGAACAGTAATAGGGTGCTAACCGGAGATTTAGAGGCCAATTCAGGTGATAGCCCTTCCATAAAGTCTGATTATGAGGGAGTGGATAGCCGTCAATTTGAATTCAGTGATAATGGAAGAAATCCAGTATCGGTTAAACAAAACTCGGGGGTGCAAATGAACACGGATGGATCTGAAGGCCTTCCATCACCATCAGGAAGTGCTTCAAAATCTGCTGAAGGTCGAAGCCTCCAACGGAGTCAAACAATAGGTCAAAGGATTATGGACAATCTCTTTCTTAGAAATCTTACTTCTAAGCCTAAGGCTTTTCCATCCGAGGTTTCAGTACCATCTTCTCCAATTAGGGTGCCTGAGGCTGTCGAACCTGATATCAGAGATGAGGAGGAAAGTTCACTGTTGGGAACTTGTAGGGGCAAATGCATCACTCAACTTTTACTTCTTGGTGCCATTGATGGTATTCAGAAGAAATACTGGAGCAACTTGAAAACACCACAAAAGATTGCTATTATGGATATTTTGCTGTCTGCATTAGAGTTCGCAGCTTCGTACAATTCATATACCAATCTCAGAACACGCATGCACCAAGTTCATGATGAGAGGCCACCTCTGAATCTTCTCCGCCAAGAATTAGCAGGAACTTGTATATATCTGGATCGATCTTACAGAAAGCAACCTCCGGGTTTAGTGCTGACAAAGAGGGAGAAACTAATGGTGAAGAGAAAGTCGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

966

Amino Acids

108.17

Weight (kDa)

6.01

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7 PF01369 1 - 80 3.3e-24 Sec7 domain
Sec7-like_HDS PF09324 393 - 475 2.3e-27 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 549 - 614 1.2e-07 C-terminal region of Mon2 protein
BIG2_C PF20252 863 - 938 3.4e-06 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 89, 1932
Acc16I TGCGCA 2 cut(s) 108, 1553
AccB1I GGYRCC 2 cut(s) 2509, 2606
AccBSI CCGCTC 1 cut(s) 1189
AccI GTMKAC 2 cut(s) 1057, 1969
AccII CGCG 1 cut(s) 83
AciI CCGC 6 cut(s) 83, 167, 310, 1189, 1193, 2782
AclI AACGTT 1 cut(s) 1350
AclWI GGATC 6 cut(s) 430, 841, 1572, 1688, 2341, 2823
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 3 cut(s) 8, 1763, 2264
AcuI CTGAAG 4 cut(s) 999, 2183, 2358, 2400
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 3 cut(s) 2114, 2490, 2711
AfiI CCNNNNNNNGG 3 cut(s) 331, 1323, 1666
AflIII ACRYGT 3 cut(s) 595, 1831, 1923
AhdI GACNNNNNGTC 2 cut(s) 261, 1706
AjnI CCWGG 2 cut(s) 37, 1588
AjuI GAANNNNNNNTTGG 4 cut(s) 1196, 1209, 1228, 1241
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
Alw21I GWGCWC 2 cut(s) 190, 2029
Alw26I GTCTC 2 cut(s) 248, 1822
Alw44I GTGCAC 1 cut(s) 2025
AlwI GGATC 6 cut(s) 430, 841, 1572, 1688, 2341, 2823
AlwNI CAGNNNCTG 4 cut(s) 62, 734, 1373, 1716
Ama87I CYCGRG 1 cut(s) 2308
AoxI GGCC 8 cut(s) 125, 307, 470, 494, 1604, 2199, 2341, 2762
ApaLI GTGCAC 1 cut(s) 2025
ApeKI GCWGC 7 cut(s) 164, 815, 1165, 1370, 1373, 1897, 2702
ApoI RAATTY 3 cut(s) 8, 1763, 2264
ArsI GACNNNNNNTTYG 2 cut(s) 2398, 2430
Asp700I GAANNNNTTC 3 cut(s) 892, 2343, 2365
AspLEI GCGC 4 cut(s) 109, 352, 1554, 1723
AsuC2I CCSGG 1 cut(s) 2841
AsuHPI GGTGA 5 cut(s) 308, 545, 2222, 2343, 2887
AvaI CYCGRG 1 cut(s) 2308
AxyI CCTNAGG 3 cut(s) 1160, 2463, 2514
BaeGI GKGCMC 1 cut(s) 2029
BalI TGGCCA 1 cut(s) 127
BanI GGYRCC 2 cut(s) 2509, 2606
BauI CACGAG 1 cut(s) 1002
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 2 cut(s) 190, 2029
BbvI GCAGC 7 cut(s) 151, 827, 1177, 1357, 1360, 1884, 2714
BccI CCATC 8 cut(s) 392, 1335, 2324, 2356, 2362, 2482, 2500, 2609
BceAI ACGGC 1 cut(s) 2238
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BciT130I CCWGG 2 cut(s) 39, 1590
BciVI GTATCC 3 cut(s) 332, 1618, 2074
BclI TGATCA 1 cut(s) 1996
BcnI CCSGG 1 cut(s) 2841
BcoDI GTCTC 2 cut(s) 248, 1822
BfaI CTAG 3 cut(s) 464, 930, 1074
BfmI CTRYAG 5 cut(s) 486, 810, 939, 1374, 1898
BfuI GTATCC 3 cut(s) 332, 1618, 2074
BisI GCNGC 8 cut(s) 165, 310, 816, 1166, 1371, 1374, 1898, 2703
BlsI GCNGC 8 cut(s) 166, 311, 817, 1167, 1372, 1375, 1899, 2704
Bme1390I CCNGG 3 cut(s) 39, 1590, 2841
BmeRI GACNNNNNGTC 2 cut(s) 261, 1706
BmeT110I CYCGRG 1 cut(s) 2308
BmiI GGNNCC 6 cut(s) 416, 739, 1658, 1874, 2511, 2608
BmrFI CCNGG 3 cut(s) 39, 1590, 2841
BmrI ACTGGG 1 cut(s) 2086
BmsI GCATC 8 cut(s) 94, 163, 844, 1305, 1338, 2044, 2095, 2592
BmuI ACTGGG 1 cut(s) 2086
BpiI GAAGAC 1 cut(s) 430
BplI GAGNNNNNCTC 2 cut(s) 2752, 2784
BpmI CTGGAG 3 cut(s) 981, 1205, 2656
BpuEI CTTGAG 2 cut(s) 287, 806
BpuMI CCSGG 1 cut(s) 2841
Bsa29I ATCGAT 1 cut(s) 2818
BsaJI CCNNGG 2 cut(s) 418, 2478
BsaWI WCCGGW 1 cut(s) 2186
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 1323, 1666
Bse1I ACTGG 5 cut(s) 739, 1658, 2081, 2288, 2639
Bse21I CCTNAGG 3 cut(s) 1160, 2463, 2514
BseBI CCWGG 2 cut(s) 39, 1590
BseCI ATCGAT 1 cut(s) 2818
BseDI CCNNGG 2 cut(s) 418, 2478
BseGI GGATG 4 cut(s) 175, 1346, 2335, 2474
BseLI CCNNNNNNNGG 3 cut(s) 331, 1323, 1666
BseMII CTCAG 5 cut(s) 741, 1151, 1978, 2505, 2743
BseNI ACTGG 5 cut(s) 739, 1658, 2081, 2288, 2639
BseRI GAGGAG 2 cut(s) 542, 2558
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 7 cut(s) 151, 827, 1177, 1357, 1360, 1884, 2714
BseYI CCCAGC 1 cut(s) 1112
BsgI GTGCAG 3 cut(s) 48, 432, 942
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 1450
BshFI GGCC 8 cut(s) 127, 309, 472, 496, 1606, 2201, 2343, 2764
BshNI GGYRCC 2 cut(s) 2509, 2606
BshVI ATCGAT 1 cut(s) 2818
BsiEI CGRYCG 1 cut(s) 1450
BsiHKAI GWGCWC 2 cut(s) 190, 2029
BsiHKCI CYCGRG 1 cut(s) 2308
BsiSI CCGG 2 cut(s) 2187, 2840
BslFI GGGAC 1 cut(s) 745
BslI CCNNNNNNNGG 3 cut(s) 331, 1323, 1666
BsmAI GTCTC 2 cut(s) 248, 1822
BsmFI GGGAC 1 cut(s) 745
BsmI GAATGC 3 cut(s) 166, 763, 1887
BsnI GGCC 8 cut(s) 127, 309, 472, 496, 1606, 2201, 2343, 2764
BsoBI CYCGRG 1 cut(s) 2308
Bsp1286I GDGCHC 2 cut(s) 190, 2029
Bsp1407I TGTACA 1 cut(s) 2112
BspACI CCGC 6 cut(s) 83, 167, 310, 1189, 1193, 2782
BspANI GGCC 8 cut(s) 127, 309, 472, 496, 1606, 2201, 2343, 2764
BspCNI CTCAG 5 cut(s) 740, 1152, 1977, 2506, 2742
BspDI ATCGAT 1 cut(s) 2818
BspFNI CGCG 1 cut(s) 83
BspHI TCATGA 4 cut(s) 156, 1123, 1356, 2752
BspLI GGNNCC 6 cut(s) 416, 739, 1658, 1874, 2511, 2608
BspMAI CTGCAG 2 cut(s) 1378, 1902
BspPI GGATC 6 cut(s) 430, 841, 1572, 1688, 2341, 2823
BspQI GCTCTTC 2 cut(s) 342, 864
BspT107I GGYRCC 2 cut(s) 2509, 2606
BsrBI CCGCTC 1 cut(s) 1189
BsrGI TGTACA 1 cut(s) 2112
BsrI ACTGG 5 cut(s) 739, 1658, 2081, 2288, 2639
BssECI CCNNGG 2 cut(s) 418, 2478
BssNAI GTATAC 1 cut(s) 1058
BssSI CACGAG 1 cut(s) 1002
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 1058
Bst2BI CACGAG 1 cut(s) 1002
Bst2UI CCWGG 2 cut(s) 39, 1590
Bst4CI ACNGT 6 cut(s) 823, 1478, 1487, 1523, 2171, 2559
Bst6I CTCTTC 6 cut(s) 174, 228, 294, 342, 864, 2873
BstAPI GCANNNNNTGC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 2112
BstC8I GCNNGC 6 cut(s) 504, 663, 1016, 1191, 1668, 2741
BstDEI CTNAG 9 cut(s) 366, 727, 1160, 1964, 2441, 2457, 2463, 2514, 2729
BstF5I GGATG 4 cut(s) 175, 1346, 2335, 2474
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 4 cut(s) 109, 352, 1554, 1723
BstMAI GTCTC 2 cut(s) 248, 1822
BstMCI CGRYCG 1 cut(s) 1450
BstMWI GCNNNNNNNGC 7 cut(s) 62, 173, 460, 469, 1145, 1199, 1903
BstNI CCWGG 2 cut(s) 39, 1590
BstNSI RCATGY 4 cut(s) 599, 665, 1835, 2743
BstSCI CCNGG 3 cut(s) 37, 1588, 2839
BstSFI CTRYAG 5 cut(s) 486, 810, 939, 1374, 1898
BstSLI GKGCMC 1 cut(s) 2029
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 7 cut(s) 151, 827, 1177, 1357, 1360, 1884, 2714
BstV2I GAAGAC 1 cut(s) 430
BstX2I RGATCY 2 cut(s) 1693, 2333
BstXI CCANNNNNNTGG 1 cut(s) 123
BstYI RGATCY 2 cut(s) 1693, 2333
BstZ17I GTATAC 1 cut(s) 1058
Bsu15I ATCGAT 1 cut(s) 2818
Bsu36I CCTNAGG 3 cut(s) 1160, 2463, 2514
BsuI GTATCC 3 cut(s) 332, 1618, 2074
BsuRI GGCC 8 cut(s) 127, 309, 472, 496, 1606, 2201, 2343, 2764
BsuTUI ATCGAT 1 cut(s) 2818
BtsCI GGATG 4 cut(s) 175, 1346, 2335, 2474
BtsI GCAGTG 3 cut(s) 60, 698, 954
BtsIMutI CAGTG 5 cut(s) 60, 698, 954, 2275, 2555
Cac8I GCNNGC 6 cut(s) 504, 663, 1016, 1191, 1668, 2741
CaiI CAGNNNCTG 4 cut(s) 62, 734, 1373, 1716
CciI TCATGA 4 cut(s) 156, 1123, 1356, 2752
CfoI GCGC 4 cut(s) 109, 352, 1554, 1723
ClaI ATCGAT 1 cut(s) 2818
CsiI ACCWGGT 1 cut(s) 1588
Csp6I GTAC 3 cut(s) 2113, 2489, 2710
CviQI GTAC 3 cut(s) 2113, 2489, 2710
DdeI CTNAG 9 cut(s) 366, 727, 1160, 1964, 2441, 2457, 2463, 2514, 2729
DraIII CACNNNGTG 1 cut(s) 965
DriI GACNNNNNGTC 2 cut(s) 261, 1706
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 6 cut(s) 174, 228, 294, 342, 864, 2873
Eam1105I GACNNNNNGTC 2 cut(s) 261, 1706
EarI CTCTTC 6 cut(s) 174, 228, 294, 342, 864, 2873
EciI GGCGGA 2 cut(s) 1208, 2771
Eco130I CCWWGG 1 cut(s) 418
Eco147I AGGCCT 3 cut(s) 472, 1606, 2343
Eco32I GATATC 1 cut(s) 2533
Eco57I CTGAAG 4 cut(s) 999, 2183, 2358, 2400
Eco81I CCTNAGG 3 cut(s) 1160, 2463, 2514
Eco88I CYCGRG 1 cut(s) 2308
EcoRI GAATTC 1 cut(s) 2264
EcoRII CCWGG 2 cut(s) 37, 1588
EcoRV GATATC 1 cut(s) 2533
EcoT14I CCWWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 2585
ErhI CCWWGG 1 cut(s) 418
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 2092, 2124
FaqI GGGAC 1 cut(s) 745
FauI CCCGC 1 cut(s) 1182
FauNDI CATATG 1 cut(s) 1556
FbaI TGATCA 1 cut(s) 1996
FblI GTMKAC 2 cut(s) 1057, 1969
Fnu4HI GCNGC 8 cut(s) 165, 310, 816, 1166, 1371, 1374, 1898, 2703
FokI GGATG 4 cut(s) 182, 1353, 2342, 2461
Fsp4HI GCNGC 8 cut(s) 165, 310, 816, 1166, 1371, 1374, 1898, 2703
FspAI RTGCGCAY 1 cut(s) 108
FspBI CTAG 3 cut(s) 464, 930, 1074
FspI TGCGCA 2 cut(s) 108, 1553
GlaI GCGC 4 cut(s) 108, 351, 1553, 1722
GluI GCNGC 8 cut(s) 165, 310, 816, 1166, 1371, 1374, 1898, 2703
GsaI CCCAGC 1 cut(s) 1116
GsuI CTGGAG 3 cut(s) 981, 1205, 2656
HaeIII GGCC 8 cut(s) 127, 309, 472, 496, 1606, 2201, 2343, 2764
HapII CCGG 2 cut(s) 2187, 2840
HhaI GCGC 4 cut(s) 109, 352, 1554, 1723
Hin6I GCGC 4 cut(s) 107, 350, 1552, 1721
HinP1I GCGC 4 cut(s) 107, 350, 1552, 1721
HincII GTYRAC 2 cut(s) 1291, 1483
HindII GTYRAC 2 cut(s) 1291, 1483
HinfI GANTC 8 cut(s) 725, 935, 1117, 1154, 1391, 2158, 2401, 2773
HpaII CCGG 2 cut(s) 2187, 2840
HphI GGTGA 5 cut(s) 308, 545, 2222, 2343, 2887
Hpy166II GTNNAC 7 cut(s) 1058, 1291, 1483, 1970, 2027, 2115, 2555
Hpy8I GTNNAC 7 cut(s) 1058, 1291, 1483, 1970, 2027, 2115, 2555
HpyCH4III ACNGT 6 cut(s) 823, 1478, 1487, 1523, 2171, 2559
HpyCH4IV ACGT 3 cut(s) 1350, 1614, 1925
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 173, 460, 469, 1145, 1199, 1903
HpyF3I CTNAG 9 cut(s) 366, 727, 1160, 1964, 2441, 2457, 2463, 2514, 2729
HpySE526I ACGT 3 cut(s) 1350, 1614, 1925
HspAI GCGC 4 cut(s) 107, 350, 1552, 1721
Ksp22I TGATCA 1 cut(s) 1996
LguI GCTCTTC 2 cut(s) 342, 864
LmnI GCTCC 7 cut(s) 193, 670, 743, 1186, 1278, 1326, 2637
Lsp1109I GCAGC 7 cut(s) 151, 827, 1177, 1357, 1360, 1884, 2714
LweI GCATC 8 cut(s) 94, 163, 844, 1305, 1338, 2044, 2095, 2592
MabI ACCWGGT 1 cut(s) 1588
MaeI CTAG 3 cut(s) 464, 930, 1074
MaeII ACGT 3 cut(s) 1350, 1614, 1925
MaeIII GTNAC 5 cut(s) 517, 722, 793, 1346, 1639
MbiI CCGCTC 1 cut(s) 1189
MfeI CAATTG 2 cut(s) 222, 1395
MflI RGATCY 2 cut(s) 1693, 2333
MhlI GDGCHC 2 cut(s) 190, 2029
MlsI TGGCCA 1 cut(s) 127
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 3 cut(s) 719, 1126, 2410
MmeI TCCRAC 5 cut(s) 215, 1438, 1816, 2029, 2419
Mox20I TGGCCA 1 cut(s) 127
Mph1103I ATGCAT 1 cut(s) 2585
MroXI GAANNNNTTC 3 cut(s) 892, 2343, 2365
MscI TGGCCA 1 cut(s) 127
MseI TTAA 8 cut(s) 228, 327, 390, 632, 872, 1230, 1283, 2298
MslI CAYNNNNRTG 1 cut(s) 1125
Msp20I TGGCCA 1 cut(s) 127
MspA1I CMGCKG 3 cut(s) 977, 1112, 1370
MspI CCGG 2 cut(s) 2187, 2840
MspR9I CCNGG 3 cut(s) 39, 1590, 2841
MunI CAATTG 2 cut(s) 222, 1395
Mva1269I GAATGC 3 cut(s) 166, 763, 1887
MvaI CCWGG 2 cut(s) 39, 1590
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 7 cut(s) 62, 173, 460, 469, 1145, 1199, 1903
NciI CCSGG 1 cut(s) 2841
NdeI CATATG 1 cut(s) 1556
NlaIV GGNNCC 6 cut(s) 416, 739, 1658, 1874, 2511, 2608
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 2 cut(s) 722, 1639
NsbI TGCGCA 2 cut(s) 108, 1553
NsiI ATGCAT 1 cut(s) 2585
NspI RCATGY 4 cut(s) 599, 665, 1835, 2743
PaeI GCATGC 2 cut(s) 665, 2743
PagI TCATGA 4 cut(s) 156, 1123, 1356, 2752
PceI AGGCCT 3 cut(s) 472, 1606, 2343
PciI ACATGT 2 cut(s) 595, 1831
PciSI GCTCTTC 2 cut(s) 342, 864
PctI GAATGC 3 cut(s) 166, 763, 1887
PdmI GAANNNNTTC 3 cut(s) 892, 2343, 2365
PfeI GAWTC 5 cut(s) 935, 1154, 1391, 2158, 2773
PflFI GACNNNGTC 1 cut(s) 1478
PkrI GCNGC 8 cut(s) 166, 311, 817, 1167, 1372, 1375, 1899, 2704
Ple19I CGATCG 1 cut(s) 1450
PleI GAGTC 3 cut(s) 719, 1125, 2409
PpsI GAGTC 3 cut(s) 719, 1125, 2409
PscI ACATGT 2 cut(s) 595, 1831
PsiI TTATAA 2 cut(s) 89, 1932
Psp1406I AACGTT 1 cut(s) 1350
Psp6I CCWGG 2 cut(s) 37, 1588
PspFI CCCAGC 1 cut(s) 1112
PspGI CCWGG 2 cut(s) 37, 1588
PspN4I GGNNCC 6 cut(s) 416, 739, 1658, 1874, 2511, 2608
PstI CTGCAG 2 cut(s) 1378, 1902
PstNI CAGNNNCTG 4 cut(s) 62, 734, 1373, 1716
PsuI RGATCY 2 cut(s) 1693, 2333
PsyI GACNNNGTC 1 cut(s) 1478
PvuI CGATCG 1 cut(s) 1450
PvuII CAGCTG 3 cut(s) 977, 1112, 1370
RsaI GTAC 3 cut(s) 2114, 2490, 2711
RsaNI GTAC 3 cut(s) 2113, 2489, 2710
RseI CAYNNNNRTG 1 cut(s) 1125
SapI GCTCTTC 2 cut(s) 342, 864
SaqAI TTAA 8 cut(s) 228, 327, 390, 632, 872, 1230, 1283, 2298
SatI GCNGC 8 cut(s) 165, 310, 816, 1166, 1371, 1374, 1898, 2703
SchI GAGTC 3 cut(s) 719, 1126, 2410
ScrFI CCNGG 3 cut(s) 39, 1590, 2841
SduI GDGCHC 2 cut(s) 190, 2029
SexAI ACCWGGT 1 cut(s) 1588
SfaNI GCATC 8 cut(s) 94, 163, 844, 1305, 1338, 2044, 2095, 2592
SfcI CTRYAG 5 cut(s) 486, 810, 939, 1374, 1898
SmiMI CAYNNNNRTG 1 cut(s) 1125
SmlI CTYRAG 2 cut(s) 266, 785
SmoI CTYRAG 2 cut(s) 266, 785
SphI GCATGC 2 cut(s) 665, 2743
SseBI AGGCCT 3 cut(s) 472, 1606, 2343
SsiI CCGC 6 cut(s) 83, 167, 310, 1189, 1193, 2782
SspI AATATT 2 cut(s) 93, 1815
SspMI CTAG 3 cut(s) 464, 930, 1074
StuI AGGCCT 3 cut(s) 472, 1606, 2343
StyD4I CCNGG 3 cut(s) 37, 1588, 2839
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 6 cut(s) 823, 1478, 1487, 1523, 2171, 2559
TaiI ACGT 3 cut(s) 1353, 1617, 1928
TatI WGTACW 1 cut(s) 2112
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 5 cut(s) 935, 1154, 1391, 2158, 2773
Tru1I TTAA 8 cut(s) 228, 327, 390, 632, 872, 1230, 1283, 2298
Tru9I TTAA 8 cut(s) 228, 327, 390, 632, 872, 1230, 1283, 2298
TscAI CASTG 5 cut(s) 67, 705, 961, 2275, 2562
TseFI GTSAC 2 cut(s) 722, 1639
TseI GCWGC 7 cut(s) 164, 815, 1165, 1370, 1373, 1897, 2702
Tsp45I GTSAC 2 cut(s) 722, 1639
TspGWI ACGGA 4 cut(s) 346, 1865, 2342, 2413
TspRI CASTG 5 cut(s) 67, 705, 961, 2275, 2562
Tth111I GACNNNGTC 1 cut(s) 1478
VneI GTGCAC 1 cut(s) 2025
XapI RAATTY 3 cut(s) 8, 1763, 2264
XceI RCATGY 4 cut(s) 599, 665, 1835, 2743
XmiI GTMKAC 2 cut(s) 1057, 1969
XmnI GAANNNNTTC 3 cut(s) 892, 2343, 2365
XspI CTAG 3 cut(s) 464, 930, 1074
Zsp2I ATGCAT 1 cut(s) 2585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.