pycom15g22130

RTE1-HOMOLOG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005598
Physical Location & Seq
Forward (+)
16230735 .. 16231414
680 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGCTGTATTGTGTGGACACCCCTTCCTGTTGTTTCATGGTTGATTCCTTTTGTTGGCCACATTGGCATATGCAGAGAAGATGGTGTGATTTGGACTTTGCGGGGCCCAATTTCGTGTGCGTGGATAACTTTGCTTTTGGAGCAGCGACTTGCTACATCCAAATAAGCAAAGAAAAGTGTTGCGCTATTCCCGATCCGTCTGAGTACCTAAGTGAGGATCAATATGGGCAGAATGAACCTGGAAGCGACATAAAGACATGGGATGATGCGCTCCGGAAGGGCACTCAGGAATTCCAACCCCGAGCTTACAACCTTTTTACCTGCAATTCCCACTCCTTTGTAGCCAACAACCTAAACAGGTTAGGCTTTCGGTCTGGTGGGTGGAATGTGGTGAACCTTGCAGCTCTGATCTTTCTCAAGGATCAGTGGGTGAGCACAGCAGCCGTGGTGCAATCCTTACTACCGTTTTTAATAACATTCTGTCTCGGAGTTGCATTAGCAGGTTCAACCTTCCTAAAGTACTTAGGTTTCTTCACCGCCTTTCTTGTCGGTTGGTTTCTTCTTGGTACGTACTGCTCCAAGAGATTGATCCAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
DUF778

Protein Analysis

200

Amino Acids

22.55

Weight (kDa)

5.86

Isoelectric Point (pI)

30.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 31 - 140 1.9e-36 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 329, 491
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 2 cut(s) 101, 537
AclWI GGATC 4 cut(s) 188, 225, 429, 583
AcoI YGGCCR 1 cut(s) 56
AcsI RAATTY 1 cut(s) 290
AfaI GTAC 4 cut(s) 206, 521, 568, 572
AfiI CCNNNNNNNGG 2 cut(s) 54, 214
AgsI TTSAA 1 cut(s) 507
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 2 cut(s) 305, 404
AluI AGCT 2 cut(s) 305, 404
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 4 cut(s) 188, 225, 429, 583
Ama87I CYCGRG 1 cut(s) 300
Aor13HI TCCGGA 1 cut(s) 273
AoxI GGCC 2 cut(s) 56, 104
ApaI GGGCCC 1 cut(s) 108
ApeKI GCWGC 3 cut(s) 143, 401, 440
ApoI RAATTY 1 cut(s) 290
AspLEI GCGC 2 cut(s) 185, 271
AspS9I GGNCC 2 cut(s) 104, 105
AsuHPI GGTGA 3 cut(s) 403, 442, 526
AvaI CYCGRG 1 cut(s) 300
BaeGI GKGCMC 2 cut(s) 108, 284
BalI TGGCCA 1 cut(s) 58
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 155, 413, 452
BccI CCATC 1 cut(s) 75
BceAI ACGGC 1 cut(s) 428
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 488
BfuAI ACCTGC 2 cut(s) 329, 491
BglI GCCNNNNNGGC 1 cut(s) 64
BisI GCNGC 3 cut(s) 144, 402, 441
BlsI GCNGC 3 cut(s) 145, 403, 442
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 1 cut(s) 240
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 104, 105
BmiI GGNNCC 2 cut(s) 105, 106
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 444
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 214
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 2 cut(s) 156, 268
BseLI CCNNNNNNNGG 2 cut(s) 54, 214
BseMII CTCAG 2 cut(s) 192, 299
BseSI GKGCMC 2 cut(s) 108, 284
BseXI GCAGC 3 cut(s) 155, 413, 452
BshFI GGCC 2 cut(s) 58, 106
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 54, 214
BsmAI GTCTC 1 cut(s) 488
BsnI GGCC 2 cut(s) 58, 106
BsoBI CYCGRG 1 cut(s) 300
Bsp120I GGGCCC 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 108, 284, 437
Bsp13I TCCGGA 1 cut(s) 273
Bsp143I GATC 5 cut(s) 193, 217, 408, 421, 588
BspACI CCGC 2 cut(s) 101, 537
BspANI GGCC 2 cut(s) 58, 106
BspCNI CTCAG 2 cut(s) 193, 298
BspEI TCCGGA 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 105, 106
BspMI ACCTGC 2 cut(s) 329, 491
BspPI GGATC 4 cut(s) 188, 225, 429, 583
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 5 cut(s) 193, 217, 408, 421, 588
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 570
BstDEI CTNAG 4 cut(s) 201, 209, 285, 523
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 2 cut(s) 156, 268
BstHHI GCGC 2 cut(s) 185, 271
BstKTI GATC 5 cut(s) 196, 220, 411, 424, 591
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 5 cut(s) 193, 217, 408, 421, 588
BstMWI GCNNNNNNNGC 2 cut(s) 64, 140
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 238
BstSLI GKGCMC 2 cut(s) 108, 284
BstSNI TACGTA 1 cut(s) 570
BstV1I GCAGC 3 cut(s) 155, 413, 452
BsuRI GGCC 2 cut(s) 58, 106
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 156, 268
BtsIMutI CAGTG 1 cut(s) 431
BveI ACCTGC 2 cut(s) 329, 491
CfoI GCGC 2 cut(s) 185, 271
Cfr13I GGNCC 2 cut(s) 104, 105
Csp6I GTAC 4 cut(s) 205, 520, 567, 571
CviAII CATG 2 cut(s) 37, 258
CviJI RGCY 7 cut(s) 58, 106, 305, 344, 366, 404, 443
CviKI_1 RGCY 7 cut(s) 58, 106, 305, 344, 366, 404, 443
CviQI GTAC 4 cut(s) 205, 520, 567, 571
DdeI CTNAG 4 cut(s) 201, 209, 285, 523
DpnI GATC 5 cut(s) 195, 219, 410, 423, 590
DpnII GATC 5 cut(s) 193, 217, 408, 421, 588
EaeI YGGCCR 1 cut(s) 56
Eco105I TACGTA 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 2 cut(s) 40, 261
FaiI YATR 6 cut(s) 38, 69, 71, 225, 251, 259
FatI CATG 2 cut(s) 36, 257
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 144, 402, 441
FokI GGATG 2 cut(s) 143, 275
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 3 cut(s) 144, 402, 441
GlaI GCGC 2 cut(s) 184, 270
GluI GCNGC 3 cut(s) 144, 402, 441
HaeIII GGCC 2 cut(s) 58, 106
HapII CCGG 1 cut(s) 274
HhaI GCGC 2 cut(s) 185, 271
Hin1II CATG 2 cut(s) 40, 261
Hin6I GCGC 2 cut(s) 183, 269
HinP1I GCGC 2 cut(s) 183, 269
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 274
HphI GGTGA 3 cut(s) 403, 442, 526
Hpy166II GTNNAC 2 cut(s) 16, 394
Hpy188I TCNGA 3 cut(s) 202, 408, 488
Hpy188III TCNNGA 3 cut(s) 191, 274, 287
Hpy8I GTNNAC 2 cut(s) 16, 394
HpyAV CCTTC 3 cut(s) 33, 271, 520
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4IV ACGT 1 cut(s) 569
HpyCH4V TGCA 5 cut(s) 73, 324, 401, 451, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 140
HpyF3I CTNAG 4 cut(s) 201, 209, 285, 523
HpySE526I ACGT 1 cut(s) 569
Hsp92II CATG 2 cut(s) 40, 261
HspAI GCGC 2 cut(s) 183, 269
Kpn2I TCCGGA 1 cut(s) 273
Kzo9I GATC 5 cut(s) 193, 217, 408, 421, 588
LmnI GCTCC 3 cut(s) 140, 276, 581
LpnPI CCDG 9 cut(s) 40, 225, 252, 272, 287, 334, 343, 360, 486
Lsp1109I GCAGC 3 cut(s) 155, 413, 452
LweI GCATC 1 cut(s) 256
MaeII ACGT 1 cut(s) 569
MalI GATC 5 cut(s) 195, 219, 410, 423, 590
MboI GATC 5 cut(s) 193, 217, 408, 421, 588
MboII GAAGA 3 cut(s) 90, 523, 551
MfeI CAATTG 1 cut(s) 593
MhlI GDGCHC 3 cut(s) 108, 284, 437
MlsI TGGCCA 1 cut(s) 58
MluCI AATT 4 cut(s) 109, 290, 325, 593
MluNI TGGCCA 1 cut(s) 58
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 1 cut(s) 208
Mox20I TGGCCA 1 cut(s) 58
MroI TCCGGA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 58
MseI TTAA 1 cut(s) 470
Msp20I TGGCCA 1 cut(s) 58
MspI CCGG 1 cut(s) 274
MspR9I CCNGG 1 cut(s) 240
MunI CAATTG 1 cut(s) 593
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 64, 140
NdeI CATATG 1 cut(s) 69
NdeII GATC 5 cut(s) 193, 217, 408, 421, 588
NlaIII CATG 2 cut(s) 40, 261
NlaIV GGNNCC 2 cut(s) 105, 106
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 3 cut(s) 145, 403, 442
Ppu21I YACGTR 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 105, 106
PspOMI GGGCCC 1 cut(s) 104
PspPI GGNCC 2 cut(s) 104, 105
RsaI GTAC 4 cut(s) 206, 521, 568, 572
RsaNI GTAC 4 cut(s) 205, 520, 567, 571
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 3 cut(s) 144, 402, 441
Sau3AI GATC 5 cut(s) 193, 217, 408, 421, 588
Sau96I GGNCC 2 cut(s) 104, 105
ScaI AGTACT 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 240
SduI GDGCHC 3 cut(s) 108, 284, 437
SfaNI GCATC 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SnaBI TACGTA 1 cut(s) 570
Sse9I AATT 4 cut(s) 109, 290, 325, 593
SsiI CCGC 2 cut(s) 101, 537
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 465
TaiI ACGT 1 cut(s) 572
TaqII GACCGA 1 cut(s) 360
TasI AATT 4 cut(s) 109, 290, 325, 593
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 1 cut(s) 431
TseI GCWGC 3 cut(s) 143, 401, 440
TspDTI ATGAA 2 cut(s) 25, 249
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 431
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.