pycom15g22220

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009122
Physical Location & Seq
Reverse (-)
16323215 .. 16324279
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGGGAGCAAGTTTGAGTGGCTCCAACAACAACATCCCAGCCCCAAACAACACATTCCAAACTATTCTCACCAAGTTCAAGTTGAAAAAGCTCAACATTATTGATCTTGTTGCACTATCTGGAAGCCACACCATTGGAAATGCCAGGTGCACCACCTTCAGGCAGAGGCTCTACAACCAGTCCGGCAACGGCCTGGCCGATTCCACCCTCGACCAGTCCTACGCCGCCCAGCTTAGAACGCGGTGCCCGAGATCCGGCGGGGACCAGAACCTGTTCTTCCTGGACTTTGTGAGCCCCACAAAGTTCGACAACAGCTACTTCAAGAACTTGCTGGCCTCCAAGGGACTTCTGAGCTCGGACGAAGTTCTGGTCACGAAAAGTGAGGTGTCGAAGAAGTTGGTGCAGCAGTATGCAGAGAACAACGAGCTTTTCTTTGAGCAGTTTGCGAAGTCCATGGTTAAGATGGGGAATCTTTCTCCGCTTACTGGGTCGATGGGAGAGATTAGGAAGCGGTGCAGGAAGGTTAACTCTTAG

Protein Analysis

178

Amino Acids

19.6

Weight (kDa)

9.74

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 8 - 140 4.9e-36 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccII CGCG 1 cut(s) 241
AciI CCGC 5 cut(s) 225, 241, 258, 479, 511
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 142
AfiI CCNNNNNNNGG 3 cut(s) 159, 254, 485
AgsI TTSAA 3 cut(s) 79, 85, 322
AjnI CCWGG 3 cut(s) 143, 192, 279
AluBI AGCT 5 cut(s) 91, 232, 315, 354, 427
AluI AGCT 5 cut(s) 91, 232, 315, 354, 427
Alw21I GWGCWC 2 cut(s) 152, 356
Alw44I GTGCAC 1 cut(s) 148
AlwI GGATC 1 cut(s) 246
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 3 cut(s) 190, 195, 333
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 403
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
Asp700I GAANNNNTTC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 2 cut(s) 152, 248
BanI GGYRCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 296, 356
Bbv12I GWGCWC 2 cut(s) 152, 356
BbvI GCAGC 1 cut(s) 415
BccI CCATC 2 cut(s) 457, 487
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 3 cut(s) 145, 194, 281
BisI GCNGC 2 cut(s) 225, 404
BlsI GCNGC 2 cut(s) 226, 405
Bme1390I CCNGG 3 cut(s) 145, 194, 281
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 3 cut(s) 22, 245, 263
BmrFI CCNGG 3 cut(s) 145, 194, 281
BmrI ACTGGG 1 cut(s) 495
BmuI ACTGGG 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 339, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 254, 485
Bse1I ACTGG 3 cut(s) 178, 214, 490
BseBI CCWGG 3 cut(s) 145, 194, 281
BseDI CCNNGG 2 cut(s) 339, 453
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 3 cut(s) 159, 254, 485
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 3 cut(s) 178, 214, 490
BseSI GKGCMC 2 cut(s) 152, 248
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 2 cut(s) 37, 228
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 3 cut(s) 192, 197, 335
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 2 cut(s) 152, 356
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 2 cut(s) 183, 255
BslFI GGGAC 2 cut(s) 275, 357
BslI CCNNNNNNNGG 3 cut(s) 159, 254, 485
BsmFI GGGAC 2 cut(s) 275, 357
BsnI GGCC 3 cut(s) 192, 197, 335
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 4 cut(s) 152, 248, 296, 356
Bsp143I GATC 2 cut(s) 103, 251
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 5 cut(s) 225, 241, 258, 479, 511
BspANI GGCC 3 cut(s) 192, 197, 335
BspCNI CTCAG 1 cut(s) 342
BspFNI CGCG 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 22, 245, 263
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 243
BsrI ACTGG 3 cut(s) 178, 214, 490
BssECI CCNNGG 2 cut(s) 339, 453
BssMI GATC 2 cut(s) 103, 251
BssT1I CCWWGG 2 cut(s) 339, 453
Bst2UI CCWGG 3 cut(s) 145, 194, 281
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 3 cut(s) 233, 350, 531
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 241
BstKTI GATC 2 cut(s) 106, 254
BstMBI GATC 2 cut(s) 103, 251
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 3 cut(s) 145, 194, 281
BstSCI CCNGG 3 cut(s) 143, 192, 279
BstSLI GKGCMC 2 cut(s) 152, 248
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 415
BstX2I RGATCY 1 cut(s) 251
BstXI CCANNNNNNTGG 1 cut(s) 134
BstYI RGATCY 1 cut(s) 251
BsuRI GGCC 3 cut(s) 192, 197, 335
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 333
CaiI CAGNNNCTG 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 262
CviAII CATG 1 cut(s) 454
DdeI CTNAG 3 cut(s) 233, 350, 531
DpnI GATC 2 cut(s) 105, 253
DpnII GATC 2 cut(s) 103, 251
EaeI YGGCCR 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 339, 453
Eco24I GRGCYC 2 cut(s) 296, 356
Eco47I GGWCC 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 354
EcoRII CCWGG 3 cut(s) 143, 192, 279
EcoT14I CCWWGG 2 cut(s) 339, 453
EcoT38I GRGCYC 2 cut(s) 296, 356
ErhI CCWWGG 2 cut(s) 339, 453
FaeI CATG 1 cut(s) 457
FaiI YATR 2 cut(s) 411, 455
FaqI GGGAC 2 cut(s) 275, 357
FatI CATG 1 cut(s) 453
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 2 cut(s) 225, 404
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 2 cut(s) 296, 356
Fsp4HI GCNGC 2 cut(s) 225, 404
GluI GCNGC 2 cut(s) 225, 404
GsaI CCCAGC 2 cut(s) 41, 232
HaeIII GGCC 3 cut(s) 192, 197, 335
HapII CCGG 2 cut(s) 183, 255
Hin1II CATG 1 cut(s) 457
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 2 cut(s) 200, 469
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 2 cut(s) 183, 255
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 150, 526
Hpy188I TCNGA 2 cut(s) 351, 358
Hpy188III TCNNGA 3 cut(s) 120, 322, 373
Hpy8I GTNNAC 2 cut(s) 150, 526
HpyAV CCTTC 2 cut(s) 166, 514
HpyCH4V TGCA 5 cut(s) 113, 150, 403, 413, 516
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 3 cut(s) 233, 350, 531
Hsp92II CATG 1 cut(s) 457
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 2 cut(s) 103, 251
LmnI GCTCC 2 cut(s) 5, 26
Lsp1109I GCAGC 1 cut(s) 415
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 105, 253
MboI GATC 2 cut(s) 103, 251
MboII GAAGA 2 cut(s) 268, 403
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 4 cut(s) 152, 248, 296, 356
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 4 cut(s) 159, 218, 346, 376
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 2 cut(s) 459, 525
MspI CCGG 2 cut(s) 183, 255
MspR9I CCNGG 3 cut(s) 145, 194, 281
MvaI CCWGG 3 cut(s) 145, 194, 281
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 453
NdeII GATC 2 cut(s) 103, 251
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 3 cut(s) 22, 245, 263
NmuCI GTSAC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 245
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 2 cut(s) 200, 469
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 2 cut(s) 226, 405
Psp124BI GAGCTC 1 cut(s) 356
Psp6I CCWGG 3 cut(s) 143, 192, 279
PspFI CCCAGC 2 cut(s) 37, 228
PspGI CCWGG 3 cut(s) 143, 192, 279
PspN4I GGNNCC 3 cut(s) 22, 245, 263
PspPI GGNCC 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 251
SacI GAGCTC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 459, 525
SatI GCNGC 2 cut(s) 225, 404
Sau3AI GATC 2 cut(s) 103, 251
Sau96I GGNCC 1 cut(s) 262
ScrFI CCNGG 3 cut(s) 145, 194, 281
SduI GDGCHC 4 cut(s) 152, 248, 296, 356
SinI GGWCC 1 cut(s) 262
SsiI CCGC 5 cut(s) 225, 241, 258, 479, 511
SstI GAGCTC 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 143, 192, 279
StyI CCWWGG 2 cut(s) 339, 453
TaqI TCGA 4 cut(s) 210, 306, 389, 491
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 2 cut(s) 200, 469
Tru1I TTAA 2 cut(s) 459, 525
Tru9I TTAA 2 cut(s) 459, 525
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 370
VneI GTGCAC 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.