pycom15g23820

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015376
Physical Location & Seq
Forward (+)
17960498 .. 17963165
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGGCCATGGGGTATAATTCTTGCACTGCGGCCTGCGTAGGGTTTGTTCTTTTGCTCGTTTTTAATCAGAGTTATTTGGGAAATGCTGCTGATCAAGGTCAAGGGTTAGGAGCTTCTTTTATTTTTGGCGATTCTCTGGTTGATGCTGGAAACAATAATTATTTACCAACGTTGTCCAAGGCGAATATGGTTCCAAACGGGATCGATTTTAAAGCATCTGGAGGAAAACCTACCGGAAGGTTTACCAATGGTAGAACCATTGGTGATATAGTTGGAGAAGAATTGGGGCAACCAAACTATGCACTCCCATATTTGTCACCAAATGCCACGGGAAAGGCCCTATTGGTTGGGGTGAATTATGCGTCAGGAGGGGCAGGGATTTTGAATGCAACAGGAAGAATATTTATTAATAGGGTGGGAATGGATATCCAAGTCGATTTTTTCAACCAGACAAGGAAGCAGATTGACAAGGTATTGGGCCCATCACAGGCAAAAGAGTACATTATGAAAAGATCCATTTTTTCAATCACAGTTGGGGCCAATGACTTTTTGAACAATTATCTCCTACCAGTCCTCTCCATTGGAACAAGAATTTCTCAGAGCCCAGATGCTTTTATTGATGATTTGATCTCTCACTTCAGAGTCCAACTTACTAGACTTTATCAATTGGATGCTCGAAAATTTGTGATTGGAAGTATTGGACCTATTGGCTGCATACCATACCAAAAGACCATAAATCAACTGAATCCAGACCAGTGTGTGGAGTTGCCAAATAAGCTAGCTCTTCAGTACAATGGCCGGTTGAAGGGCTTGGTTGCTGAACTGAATGAGAACCTTCCAGGTTCAACATTTGTTTATGCAAACGTGTATGATCTAGTTATGGACGTCATTACGAATTATGAAAAATATGGATTTACAACAGCAAGTGTAGCATGCTGTGGAAATGGTGGCCAATACGCGGGTATAATTCCATGTGGCCCACAATCCAGTCTCTGCAATGATCGGTTGATGCATGTTTTCTGGGATCCCTACCATCCAAGTGATGCTGCCAACCTTATACTCGCCAAAAAATTGCTCGATGGAGACACAAAAATAATTTATCCAATGAATCTCAGACAACTTCGAGACCATGGATTGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.17

Weight (kDa)

7.51

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 358 3.1e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 888
AccB7I CCANNNNNTGG 2 cut(s) 760, 1135
AccII CGCG 1 cut(s) 959
AciI CCGC 2 cut(s) 29, 959
AclI AACGTT 1 cut(s) 170
AclWI GGATC 4 cut(s) 209, 507, 1019, 1032
AcoI YGGCCR 3 cut(s) 3, 796, 949
AcsI RAATTY 2 cut(s) 591, 680
AcuI CTGAAG 2 cut(s) 622, 770
AcyI GRCGYC 1 cut(s) 885
AfaI GTAC 2 cut(s) 500, 791
AfiI CCNNNNNNNGG 6 cut(s) 39, 487, 760, 805, 958, 1135
AflIII ACRYGT 1 cut(s) 864
AgsI TTSAA 6 cut(s) 385, 445, 525, 553, 805, 846
AjnI CCWGG 1 cut(s) 838
AluBI AGCT 3 cut(s) 113, 778, 782
AluI AGCT 3 cut(s) 113, 778, 782
Alw26I GTCTC 3 cut(s) 995, 1077, 1119
AlwI GGATC 4 cut(s) 209, 507, 1019, 1032
AlwNI CAGNNNCTG 1 cut(s) 993
AoxI GGCC 8 cut(s) 3, 30, 336, 478, 537, 796, 949, 976
ApaI GGGCCC 1 cut(s) 482
ApeKI GCWGC 3 cut(s) 86, 711, 1046
ApoI RAATTY 2 cut(s) 591, 680
AseI ATTAAT 1 cut(s) 408
AspS9I GGNCC 6 cut(s) 337, 478, 479, 537, 701, 977
AsuHPI GGTGA 3 cut(s) 275, 309, 364
AsuNHI GCTAGC 1 cut(s) 778
AvaII GGWCC 1 cut(s) 701
BaeGI GKGCMC 1 cut(s) 482
BalI TGGCCA 2 cut(s) 5, 951
BamHI GGATCC 1 cut(s) 1024
BanII GRGCYC 2 cut(s) 482, 605
BbvI GCAGC 3 cut(s) 73, 698, 1033
BccI CCATC 3 cut(s) 490, 1041, 1073
BciT130I CCWGG 1 cut(s) 840
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 3 cut(s) 995, 1077, 1119
BfaI CTAG 3 cut(s) 654, 779, 875
BisI GCNGC 4 cut(s) 30, 87, 712, 1047
BlsI GCNGC 4 cut(s) 31, 88, 713, 1048
Bme1390I CCNGG 1 cut(s) 840
Bme18I GGWCC 1 cut(s) 701
BmgT120I GGNCC 6 cut(s) 337, 478, 479, 537, 701, 977
BmiI GGNNCC 4 cut(s) 192, 480, 538, 1026
BmrFI CCNGG 1 cut(s) 840
BmsI GCATC 6 cut(s) 133, 224, 598, 661, 999, 1033
BmtI GCTAGC 1 cut(s) 782
BpmI CTGGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 4 cut(s) 6, 177, 327, 1129
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 487, 760, 805, 958, 1135
Bse118I RCCGGY 1 cut(s) 798
Bse1I ACTGG 3 cut(s) 569, 754, 987
Bse3DI GCAATG 1 cut(s) 1003
BseBI CCWGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 4 cut(s) 6, 177, 327, 1129
BseGI GGATG 2 cut(s) 676, 1033
BseLI CCNNNNNNNGG 6 cut(s) 39, 487, 760, 805, 958, 1135
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 2 cut(s) 611, 1126
BseNI ACTGG 3 cut(s) 569, 754, 987
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 3 cut(s) 73, 698, 1033
Bsh1236I CGCG 1 cut(s) 959
BshFI GGCC 8 cut(s) 5, 32, 338, 480, 539, 798, 951, 978
BshVI ATCGAT 1 cut(s) 204
BsiSI CCGG 2 cut(s) 234, 799
BslI CCNNNNNNNGG 6 cut(s) 39, 487, 760, 805, 958, 1135
BsmAI GTCTC 3 cut(s) 995, 1077, 1119
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 8 cut(s) 5, 32, 338, 480, 539, 798, 951, 978
Bso31I GGTCTC 1 cut(s) 1119
Bsp120I GGGCCC 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 482, 605
Bsp143I GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
Bsp19I CCATGG 2 cut(s) 6, 1129
BspACI CCGC 2 cut(s) 29, 959
BspANI GGCC 8 cut(s) 5, 32, 338, 480, 539, 798, 951, 978
BspCNI CTCAG 2 cut(s) 610, 1125
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 4 cut(s) 192, 480, 538, 1026
BspOI GCTAGC 1 cut(s) 782
BspPI GGATC 4 cut(s) 209, 507, 1019, 1032
BspQI GCTCTTC 1 cut(s) 789
BspTNI GGTCTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 1003
BsrFI RCCGGY 1 cut(s) 798
BsrI ACTGG 3 cut(s) 569, 754, 987
BssAI RCCGGY 1 cut(s) 798
BssECI CCNNGG 4 cut(s) 6, 177, 327, 1129
BssMI GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
BssNI GRCGYC 1 cut(s) 885
BssT1I CCWWGG 3 cut(s) 6, 177, 1129
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 1 cut(s) 532
Bst6I CTCTTC 1 cut(s) 789
BstACI GRCGYC 1 cut(s) 885
BstC8I GCNNGC 3 cut(s) 34, 780, 934
BstDEI CTNAG 2 cut(s) 597, 1112
BstDSI CCRYGG 3 cut(s) 6, 327, 1129
BstF5I GGATG 2 cut(s) 676, 1033
BstFNI CGCG 1 cut(s) 959
BstKTI GATC 7 cut(s) 94, 204, 515, 630, 874, 1003, 1027
BstMAI GTCTC 3 cut(s) 995, 1077, 1119
BstMBI GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
BstMWI GCNNNNNNNGC 2 cut(s) 775, 929
BstNI CCWGG 1 cut(s) 840
BstNSI RCATGY 2 cut(s) 936, 1016
BstSCI CCNGG 1 cut(s) 838
BstSLI GKGCMC 1 cut(s) 482
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 3 cut(s) 73, 698, 1033
BstX2I RGATCY 2 cut(s) 512, 1024
BstYI RGATCY 2 cut(s) 512, 1024
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 8 cut(s) 5, 32, 338, 480, 539, 798, 951, 978
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 3 cut(s) 6, 327, 1129
BtsCI GGATG 2 cut(s) 676, 1033
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 761
Cac8I GCNNGC 3 cut(s) 34, 780, 934
CaiI CAGNNNCTG 1 cut(s) 993
Cfr10I RCCGGY 1 cut(s) 798
Cfr13I GGNCC 6 cut(s) 337, 478, 479, 537, 701, 977
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 1 cut(s) 351
Csp6I GTAC 2 cut(s) 499, 790
CviAII CATG 5 cut(s) 7, 933, 972, 1013, 1130
CviQI GTAC 2 cut(s) 499, 790
DdeI CTNAG 2 cut(s) 597, 1112
DpnI GATC 7 cut(s) 93, 203, 514, 629, 873, 1002, 1026
DpnII GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
DraI TTTAAA 1 cut(s) 211
EaeI YGGCCR 3 cut(s) 3, 796, 949
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Eco130I CCWWGG 3 cut(s) 6, 177, 1129
Eco24I GRGCYC 2 cut(s) 482, 605
Eco31I GGTCTC 1 cut(s) 1119
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 701
Eco57I CTGAAG 2 cut(s) 622, 770
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 838
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 3 cut(s) 6, 177, 1129
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 2 cut(s) 482, 605
ErhI CCWWGG 3 cut(s) 6, 177, 1129
FaeI CATG 5 cut(s) 10, 936, 975, 1016, 1133
FatI CATG 5 cut(s) 6, 932, 971, 1012, 1129
FauI CCCGC 1 cut(s) 952
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 4 cut(s) 30, 87, 712, 1047
FokI GGATG 2 cut(s) 683, 1020
FriOI GRGCYC 2 cut(s) 482, 605
Fsp4HI GCNGC 4 cut(s) 30, 87, 712, 1047
FspBI CTAG 3 cut(s) 654, 779, 875
GluI GCNGC 4 cut(s) 30, 87, 712, 1047
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 8 cut(s) 5, 32, 338, 480, 539, 798, 951, 978
HapII CCGG 2 cut(s) 234, 799
HgaI GACGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 885
Hin1II CATG 5 cut(s) 10, 936, 975, 1016, 1133
HinfI GANTC 4 cut(s) 131, 642, 745, 1108
HpaII CCGG 2 cut(s) 234, 799
HphI GGTGA 3 cut(s) 275, 309, 364
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 4 cut(s) 69, 600, 641, 1115
Hpy188III TCNNGA 4 cut(s) 219, 366, 749, 1124
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 231, 799, 845
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4IV ACGT 3 cut(s) 170, 864, 885
HpyCH4V TGCA 7 cut(s) 24, 302, 389, 714, 860, 996, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 775, 929
HpyF3I CTNAG 2 cut(s) 597, 1112
HpySE526I ACGT 3 cut(s) 170, 864, 885
Hsp92I GRCGYC 1 cut(s) 885
Hsp92II CATG 5 cut(s) 10, 936, 975, 1016, 1133
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 3 cut(s) 73, 698, 1033
LweI GCATC 6 cut(s) 133, 224, 598, 661, 999, 1033
MaeI CTAG 3 cut(s) 654, 779, 875
MaeII ACGT 3 cut(s) 170, 864, 885
MaeIII GTNAC 1 cut(s) 315
MalI GATC 7 cut(s) 93, 203, 514, 629, 873, 1002, 1026
MboI GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
MboII GAAGA 3 cut(s) 290, 408, 776
MfeI CAATTG 1 cut(s) 665
MflI RGATCY 2 cut(s) 512, 1024
MhlI GDGCHC 2 cut(s) 482, 605
MlsI TGGCCA 2 cut(s) 5, 951
MluNI TGGCCA 2 cut(s) 5, 951
MlyI GAGTC 1 cut(s) 651
MmeI TCCRAC 2 cut(s) 253, 670
MnlI CCTC 3 cut(s) 215, 362, 584
Mox20I TGGCCA 2 cut(s) 5, 951
Mph1103I ATGCAT 1 cut(s) 1014
MscI TGGCCA 2 cut(s) 5, 951
MseI TTAA 3 cut(s) 63, 210, 408
MslI CAYNNNNRTG 1 cut(s) 1038
Msp20I TGGCCA 2 cut(s) 5, 951
MspI CCGG 2 cut(s) 234, 799
MspR9I CCNGG 1 cut(s) 840
MunI CAATTG 1 cut(s) 665
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 1 cut(s) 840
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 2 cut(s) 775, 929
NcoI CCATGG 2 cut(s) 6, 1129
NdeII GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
NheI GCTAGC 1 cut(s) 778
NlaIII CATG 5 cut(s) 10, 936, 975, 1016, 1133
NlaIV GGNNCC 4 cut(s) 192, 480, 538, 1026
NmuCI GTSAC 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 2 cut(s) 936, 1016
PaeI GCATGC 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 789
PctI GAATGC 1 cut(s) 391
PfeI GAWTC 3 cut(s) 131, 745, 1108
PflMI CCANNNNNTGG 2 cut(s) 760, 1135
PkrI GCNGC 4 cut(s) 31, 88, 713, 1048
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
PshBI ATTAAT 1 cut(s) 408
Psp1406I AACGTT 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 838
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 4 cut(s) 192, 480, 538, 1026
PspOMI GGGCCC 1 cut(s) 478
PspPI GGNCC 6 cut(s) 337, 478, 479, 537, 701, 977
PsrI GAACNNNNNNTAC 2 cut(s) 30, 62
PstNI CAGNNNCTG 1 cut(s) 993
PsuI RGATCY 2 cut(s) 512, 1024
RsaI GTAC 2 cut(s) 500, 791
RsaNI GTAC 2 cut(s) 499, 790
RseI CAYNNNNRTG 1 cut(s) 1038
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 3 cut(s) 63, 210, 408
SatI GCNGC 4 cut(s) 30, 87, 712, 1047
Sau3AI GATC 7 cut(s) 91, 201, 512, 627, 871, 1000, 1024
Sau96I GGNCC 6 cut(s) 337, 478, 479, 537, 701, 977
SchI GAGTC 1 cut(s) 651
ScrFI CCNGG 1 cut(s) 840
SduI GDGCHC 2 cut(s) 482, 605
SfaNI GCATC 6 cut(s) 133, 224, 598, 661, 999, 1033
SinI GGWCC 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 1038
SphI GCATGC 1 cut(s) 936
SsiI CCGC 2 cut(s) 29, 959
SspI AATATT 1 cut(s) 402
SspMI CTAG 3 cut(s) 654, 779, 875
StyD4I CCNGG 1 cut(s) 838
StyI CCWWGG 3 cut(s) 6, 177, 1129
TaaI ACNGT 1 cut(s) 532
TaiI ACGT 3 cut(s) 173, 867, 888
TaqI TCGA 5 cut(s) 204, 435, 676, 1077, 1123
TatI WGTACW 2 cut(s) 498, 789
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 3 cut(s) 131, 745, 1108
Tru1I TTAA 3 cut(s) 63, 210, 408
Tru9I TTAA 3 cut(s) 63, 210, 408
TscAI CASTG 2 cut(s) 31, 761
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 3 cut(s) 86, 711, 1046
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 3 cut(s) 521, 915, 1121
TspRI CASTG 2 cut(s) 31, 761
Van91I CCANNNNNTGG 2 cut(s) 760, 1135
VpaK11BI GGWCC 1 cut(s) 701
VspI ATTAAT 1 cut(s) 408
XapI RAATTY 2 cut(s) 591, 680
XceI RCATGY 2 cut(s) 936, 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 3 cut(s) 654, 779, 875
ZraI GACGTC 1 cut(s) 886
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.