pycom15g25210

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012508
Physical Location & Seq
Reverse (-)
19588150 .. 19590879
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2346 bp
ATGAGCTCCCAAAGCCTCCATTTTTTACCATGCCTTCTTCTTCTTCTTCTCTCGGTCCAATCTTCTGCTCTCGACAGAGATGGACTTATCTTACTTTCTTTCAAGTTCTCTATCCTCCGAGACCCTCAGTCCGTTCTTGCAACCTGGAATTCGTACGACGAGACGCCGTGTTCATGGAGAGGCGTCGTTTGCGCGGCGCCGCCGACACTATCGTCGGAAAACTACCCAAGAGTCGTAGGTTTGAATCTTCCCAACTCTCAGCTTCTGGGTTCCATCCCTGCCAACCTTGGCATGATCGATCACTTTCAAAGTCTCGATCTTTCCAACAATTCCCTCAACGGGTCGCTCCCACAGTCGCTCTTCAACGCCACGAAGCTTCGGGTTCTTGATTTGTCGTACAATTTAATCTCCGGCGAGTTGCCGGACGCGGTGTCGCAGCTCACCAACCTCCAGAGTCTCAACCTCTCCGACAATGCCTTGGCGGGAAAATTGCCCATCAATCTGTCTTCACTGCCCTCTCTAACGATCATTTCTTTGAAGAATAATCATTTCTCCGGCGGCCTTCCCAGCGGGTTCGAATCCGTTCAGGTTTTGGATCTGTCTTCGAATCTGATCAACGGCTCCCTGCCGTCTGATTTCGGCGGCAACAGTCTGAGTTACTTAAACGTCTCCCACAACGGAATTTCCGGAAAAATCCCACCGCAGTTTTCCGAGAGAATTCCAGGTAACGCCAAAACCGACTTATCTGTCAACAATCTCACCGGCGAACTCCCGGAATCTCAGGTGTTCTTGAACCAAGAAACTGGGTCTTTTACCGACAATCTTGATCTGTGTGGCCCACCGACCAAGAACCCATGTCCGATTCCTTCTTCTCCTTCCTCTCCTTCCTCTTCACCCAATGCTTCGTCTCCGAATTCTCCTCCGGCAATTGCTGCAATTCCCAGGAACCCAGAAGCCGTTTCGCCGATGTCTGCAAATGAGCATTCGCAGCAGAGCCAGACCGGGCTCAGACCGGCCGCCATTGTCGGAATTGTAGCCGGCGACATAGCCGGAAGCGGAATCCTCGTGCTGATTTTCCTGTACATTTACAGATTGAAGAAGAAGAAGAGAACCAAGGCCGAGATCACAGCAACGCTGAAGAAAGAAGCTAATACGAATTCAACGCCGGTAGTCAACGACTGGTCGTCTTCCTCGTCAGAATCGAAAGGGTTCACCAGATGGTCGTGCTTGAGAAAGCGAGGCGAAGACGAAGACAGCTCCGACACGACAGCCTCCGACTCCGAAGAACATCAGACGGAGCAGAATCCCCAAAAGAGTCACGAAATCAAACGGCAGCAAGAGCAGGAGCAGAGCAAAGGCGGGACCTTGGTCACCGTCGATGGCGCAAAGGAGCTAGAACTGGAGACACTGCTCAAAGCCTCGGCGTACATTCTGGGCGCCACCGGTTCGAGCATAATGTACAAGGCAGTGCTCGAAGACGGAAGCTCGTTGGCAGTTCGGAGGATCGGGGAGCAGAGTGTGGACCGGTTCAAGGATTTCGAGAACCAAATCAAGCTCGTGGCCAAATTGGTTCACCCGAACCTGGTTCGAATTCGTGGATTCTATTGGGGGGTTGATGAGAAGCTCATCATCTATGATTTCGTCCCAAATGGCAGCCTCGCAAACGCTCGTTACAGGAAGGTGGGCTCATCGCCTTGTCATCTACCGTGGGAAGCGAGGCTCAAGATAGCGAAAGGGGTTGCCCGTGGGCTCGCGTACCTCCACGAGAAGAAGCACGTCCATGGGAACCTCAAGCCTACCAACATTCTCCTAGATGTGGACATGGAGCCCCGGATTGGTGATTTTGGACTCGAGAGACTTTTGTCCGGCGACACAAGTTACAAAATGGGTAGCTCGACAAGAAATTTTGGAAGCAAGAGATCAACGACTTCGCGGGATGGCTTTCAGGACTTTGGACTAGGACCTAGTCCAGGTCCAAGTCCAAGCCCTAGCTCCATGGGCACATCGCCTTATCATTGCCCCGAGTCGCTTCGGAGCCTAAAGCCCAACCCAAAGTGGGATGTGTACTCATTTGGGGTGATATTGCTTGAGCTGCTAACCGGGAAAGTTGTGGTTGTGGATGAGGCAGGGCTTGGAGTGGATGATAGCTGCAAGGCCTTCAGGATGGCAGATATGGCAATTAGAGGTGAATTGGAGGGCAAAGAGGAGGCCTTGTTGTCCTTGTTCAAGTTAGGGTACAATTGTGCGTCGCCTGTCCCACAAAAGAGACCAACAATGAAAGAAGCCCTACAAGTCCTGGGGAAATTCCCATCTTCTCCTTCATCATCATATTATTGTGCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

782

Amino Acids

84.36

Weight (kDa)

6.69

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 64 1.4e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 103 - 160 1.3e-08 Leucine rich repeat
LRR_8 PF13855 125 - 184 4.2e-07 Leucine rich repeat
Pkinase PF00069 478 - 636 2.6e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 632 1.1e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 650 - 764 1.3e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012508)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67280
fragaria_vesca FvH4_1g16750 FvH4_1g16750
malus_domestica MD02G1178600.v1.1 MD15G1288500.v1.1
prunus_persica Prupe.6G229200_v2.0.a1
pyrus_communis pycom02g14470 pycom15g25210
rosa_chinensis RchiOBHm_Chr2g0106651
rosa_laevigata RLG00000017526
rosa_multiflora Rmu_co8446759.1_g000001 Rmu_sc0004352.1_g000001
rosa_roxburghii Rroxscaffold_2G00136920
rosa_rugosa Rorug02G0137100
rosa_samantha Rh2AG188500 Rh2BG199500 Rh2CG193500 Rh2DG194700
rosa_wichuraiana Rw2G014810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 211, 746
AccB1I GGYRCC 2 cut(s) 196, 1436
AccII CGCG 4 cut(s) 194, 428, 1754, 1933
AccIII TCCGGA 1 cut(s) 686
AclWI GGATC 2 cut(s) 603, 1511
AcoI YGGCCR 2 cut(s) 1014, 1560
AcsI RAATTY 8 cut(s) 148, 681, 717, 913, 1156, 1590, 1903, 2303
AcuI CTGAAG 2 cut(s) 1157, 2143
AcyI GRCGYC 4 cut(s) 164, 183, 197, 1437
AfaI GTAC 8 cut(s) 155, 398, 1082, 1427, 1460, 1757, 2066, 2237
AfiI CCNNNNNNNGG 7 cut(s) 339, 1531, 1582, 1816, 1835, 1970, 2056
AgeI ACCGGT 2 cut(s) 1442, 1524
AhdI GACNNNNNGTC 3 cut(s) 127, 430, 1183
AjiI CACGTC 1 cut(s) 1777
AjnI CCWGG 6 cut(s) 143, 721, 941, 1581, 1969, 2295
Alw21I GWGCWC 2 cut(s) 8, 1473
Alw26I GTCTC 9 cut(s) 114, 155, 317, 461, 673, 912, 1397, 1849, 2260
AlwI GGATC 2 cut(s) 603, 1511
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 1850, 2021
Aor13HI TCCGGA 1 cut(s) 686
AoxI GGCC 7 cut(s) 559, 835, 1014, 1116, 1560, 2154, 2208
ApeKI GCWGC 7 cut(s) 436, 932, 988, 1333, 1653, 2092, 2148
ApoI RAATTY 8 cut(s) 148, 681, 717, 913, 1156, 1590, 1903, 2303
AsiGI ACCGGT 2 cut(s) 1442, 1524
Asp700I GAANNNNTTC 2 cut(s) 582, 1208
AspLEI GCGC 4 cut(s) 194, 199, 1385, 1439
AspS9I GGNCC 6 cut(s) 55, 836, 1362, 1522, 1961, 1973
AsuC2I CCSGG 4 cut(s) 773, 1003, 1831, 2101
AsuHPI GGTGA 9 cut(s) 433, 751, 885, 1204, 1363, 1565, 1850, 2089, 2198
AsuII TTCGAA 3 cut(s) 576, 605, 1588
AvaI CYCGRG 2 cut(s) 1850, 2021
AvaII GGWCC 5 cut(s) 55, 1362, 1522, 1961, 1973
BaeGI GKGCMC 2 cut(s) 2003, 2341
BaeI ACNNNNGTAYC 2 cut(s) 2227, 2260
BalI TGGCCA 1 cut(s) 1562
BanI GGYRCC 2 cut(s) 196, 1436
BanII GRGCYC 5 cut(s) 8, 1008, 1688, 1752, 1830
BauI CACGAG 3 cut(s) 1064, 1556, 1763
BbsI GAAGAC 6 cut(s) 498, 594, 1179, 1251, 1257, 1482
Bbv12I GWGCWC 2 cut(s) 8, 1473
BbvI GCAGC 7 cut(s) 448, 919, 1000, 1345, 1665, 2079, 2135
BccI CCATC 8 cut(s) 74, 281, 503, 1212, 1373, 1931, 2158, 2317
BceAI ACGGC 5 cut(s) 151, 613, 634, 941, 1346
BciT130I CCWGG 6 cut(s) 145, 723, 943, 1583, 1971, 2297
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 4 cut(s) 773, 1003, 1831, 2101
BcoDI GTCTC 9 cut(s) 114, 155, 317, 461, 673, 912, 1397, 1849, 2260
BfaI CTAG 5 cut(s) 1394, 1811, 1958, 1965, 1989
BfoI RGCGCY 2 cut(s) 200, 1440
Bme18I GGWCC 5 cut(s) 55, 1362, 1522, 1961, 1973
BmeRI GACNNNNNGTC 3 cut(s) 127, 430, 1183
BmeT110I CYCGRG 2 cut(s) 1850, 2021
BmgBI CACGTC 1 cut(s) 1777
BmgT120I GGNCC 6 cut(s) 55, 836, 1362, 1522, 1961, 1973
BmiI GGNNCC 9 cut(s) 198, 271, 622, 947, 1363, 1438, 1787, 1827, 2036
BmrI ACTGGG 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 813
BoxI GACNNNNGTC 2 cut(s) 1367, 1861
BpiI GAAGAC 6 cut(s) 498, 594, 1179, 1251, 1257, 1482
BpmI CTGGAG 2 cut(s) 434, 1421
Bpu14I TTCGAA 3 cut(s) 576, 605, 1588
BpuEI CTTGAG 4 cut(s) 1249, 1706, 1775, 2108
BpuMI CCSGG 4 cut(s) 773, 1003, 1831, 2101
Bsa29I ATCGAT 1 cut(s) 297
BsaHI GRCGYC 4 cut(s) 164, 183, 197, 1437
BsaI GGTCTC 2 cut(s) 114, 2260
BsaWI WCCGGW 3 cut(s) 686, 1442, 1524
BsaXI ACNNNNNCTCC 2 cut(s) 2198, 2228
Bsc4I CCNNNNNNNGG 7 cut(s) 339, 1531, 1582, 1816, 1835, 1970, 2056
Bse118I RCCGGY 6 cut(s) 761, 1012, 1037, 1165, 1442, 1524
Bse1I ACTGG 3 cut(s) 808, 1184, 1404
Bse3DI GCAATG 1 cut(s) 2014
BseAI TCCGGA 1 cut(s) 686
BseBI CCWGG 6 cut(s) 145, 723, 943, 1583, 1971, 2297
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 6 cut(s) 273, 1942, 2065, 2125, 2146, 2169
BseLI CCNNNNNNNGG 7 cut(s) 339, 1531, 1582, 1816, 1835, 1970, 2056
BseMI GCAATG 1 cut(s) 2014
BseMII CTCAG 5 cut(s) 140, 272, 644, 794, 1021
BseNI ACTGG 3 cut(s) 808, 1184, 1404
BseRI GAGGAG 2 cut(s) 909, 2219
BseSI GKGCMC 2 cut(s) 2003, 2341
BseX3I CGGCCG 1 cut(s) 1014
BseXI GCAGC 7 cut(s) 448, 919, 1000, 1345, 1665, 2079, 2135
BseYI CCCAGC 1 cut(s) 566
Bsh1236I CGCG 4 cut(s) 194, 428, 1754, 1933
Bsh1285I CGRYCG 1 cut(s) 1017
BshFI GGCC 7 cut(s) 561, 837, 1016, 1118, 1562, 2156, 2210
BshNI GGYRCC 2 cut(s) 196, 1436
BshTI ACCGGT 2 cut(s) 1442, 1524
BshVI ATCGAT 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 1017
BsiHKAI GWGCWC 2 cut(s) 8, 1473
BsiHKCI CYCGRG 2 cut(s) 1850, 2021
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 3 cut(s) 1375, 1628, 2240
BslI CCNNNNNNNGG 7 cut(s) 339, 1531, 1582, 1816, 1835, 1970, 2056
BsmAI GTCTC 9 cut(s) 114, 155, 317, 461, 673, 912, 1397, 1849, 2260
BsmBI CGTCTC 3 cut(s) 155, 673, 912
BsmFI GGGAC 3 cut(s) 1375, 1628, 2240
BsmI GAATGC 1 cut(s) 982
BsnI GGCC 7 cut(s) 561, 837, 1016, 1118, 1562, 2156, 2210
Bso31I GGTCTC 2 cut(s) 114, 2260
BsoBI CYCGRG 2 cut(s) 1850, 2021
Bsp119I TTCGAA 3 cut(s) 576, 605, 1588
Bsp1286I GDGCHC 8 cut(s) 8, 1008, 1473, 1688, 1752, 1830, 2003, 2341
Bsp13I TCCGGA 1 cut(s) 686
Bsp1407I TGTACA 2 cut(s) 1080, 1458
Bsp19I CCATGG 2 cut(s) 1780, 1995
BspANI GGCC 7 cut(s) 561, 837, 1016, 1118, 1562, 2156, 2210
BspCNI CTCAG 5 cut(s) 139, 271, 645, 793, 1020
BspDI ATCGAT 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 686
BspFNI CGCG 4 cut(s) 194, 428, 1754, 1933
BspLI GGNNCC 9 cut(s) 198, 271, 622, 947, 1363, 1438, 1787, 1827, 2036
BspPI GGATC 2 cut(s) 603, 1511
BspQI GCTCTTC 1 cut(s) 365
BspT104I TTCGAA 3 cut(s) 576, 605, 1588
BspT107I GGYRCC 2 cut(s) 196, 1436
BspTNI GGTCTC 2 cut(s) 114, 2260
BsrDI GCAATG 1 cut(s) 2014
BsrFI RCCGGY 6 cut(s) 761, 1012, 1037, 1165, 1442, 1524
BsrGI TGTACA 2 cut(s) 1080, 1458
BsrI ACTGG 3 cut(s) 808, 1184, 1404
BssAI RCCGGY 6 cut(s) 761, 1012, 1037, 1165, 1442, 1524
BssNI GRCGYC 4 cut(s) 164, 183, 197, 1437
BssSI CACGAG 3 cut(s) 1064, 1556, 1763
BssT1I CCWWGG 6 cut(s) 286, 477, 1113, 1365, 1780, 1995
Bst2BI CACGAG 3 cut(s) 1064, 1556, 1763
Bst2UI CCWGG 6 cut(s) 145, 723, 943, 1583, 1971, 2297
Bst4CI ACNGT 4 cut(s) 354, 650, 1375, 1707
Bst6I CTCTTC 3 cut(s) 365, 895, 1100
BstACI GRCGYC 4 cut(s) 164, 183, 197, 1437
BstAPI GCANNNNNTGC 1 cut(s) 932
BstAUI TGTACA 2 cut(s) 1080, 1458
BstBI TTCGAA 3 cut(s) 576, 605, 1588
BstC8I GCNNGC 2 cut(s) 1039, 1752
BstDEI CTNAG 5 cut(s) 126, 258, 653, 780, 1007
BstDSI CCRYGG 4 cut(s) 1706, 1744, 1780, 1995
BstEII GGTNACC 1 cut(s) 1369
BstENI CCTNNNNNAGG 1 cut(s) 1968
BstF5I GGATG 6 cut(s) 273, 1942, 2065, 2125, 2146, 2169
BstFNI CGCG 4 cut(s) 194, 428, 1754, 1933
BstH2I RGCGCY 2 cut(s) 200, 1440
BstHHI GCGC 4 cut(s) 194, 199, 1385, 1439
BstMAI GTCTC 9 cut(s) 114, 155, 317, 461, 673, 912, 1397, 1849, 2260
BstMCI CGRYCG 1 cut(s) 1017
BstMWI GCNNNNNNNGC 9 cut(s) 12, 189, 567, 932, 988, 1339, 1998, 2092, 2174
BstNI CCWGG 6 cut(s) 145, 723, 943, 1583, 1971, 2297
BstPAI GACNNNNGTC 2 cut(s) 1367, 1861
BstPI GGTNACC 1 cut(s) 1369
BstSLI GKGCMC 2 cut(s) 2003, 2341
BstUI CGCG 4 cut(s) 194, 428, 1754, 1933
BstV1I GCAGC 7 cut(s) 448, 919, 1000, 1345, 1665, 2079, 2135
BstV2I GAAGAC 6 cut(s) 498, 594, 1179, 1251, 1257, 1482
BstX2I RGATCY 1 cut(s) 595
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 1 cut(s) 595
BstZI CGGCCG 1 cut(s) 1014
Bsu15I ATCGAT 1 cut(s) 297
BsuRI GGCC 7 cut(s) 561, 837, 1016, 1118, 1562, 2156, 2210
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 4 cut(s) 1706, 1744, 1780, 1995
BtgZI GCGATG 2 cut(s) 1674, 1989
BtrI CACGTC 1 cut(s) 1777
BtsCI GGATG 6 cut(s) 273, 1942, 2065, 2125, 2146, 2169
BtsI GCAGTG 3 cut(s) 509, 1406, 1473
BtsIMutI CAGTG 3 cut(s) 509, 1406, 1473
Cac8I GCNNGC 2 cut(s) 1039, 1752
CaiI CAGNNNCTG 1 cut(s) 265
CfoI GCGC 4 cut(s) 194, 199, 1385, 1439
Cfr10I RCCGGY 6 cut(s) 761, 1012, 1037, 1165, 1442, 1524
Cfr13I GGNCC 6 cut(s) 55, 836, 1362, 1522, 1961, 1973
ClaI ATCGAT 1 cut(s) 297
CseI GACGC 4 cut(s) 172, 172, 434, 2235
CsiI ACCWGGT 1 cut(s) 1581
Csp6I GTAC 8 cut(s) 154, 397, 1081, 1426, 1459, 1756, 2065, 2236
CspAI ACCGGT 2 cut(s) 1442, 1524
CviAII CATG 7 cut(s) 30, 174, 292, 855, 1781, 1822, 1996
CviQI GTAC 8 cut(s) 154, 397, 1081, 1426, 1459, 1756, 2065, 2236
DdeI CTNAG 5 cut(s) 126, 258, 653, 780, 1007
DinI GGCGCC 2 cut(s) 198, 1438
DrdI GACNNNNNNGTC 2 cut(s) 211, 746
DriI GACNNNNNGTC 3 cut(s) 127, 430, 1183
DseDI GACNNNNNNGTC 2 cut(s) 211, 746
EaeI YGGCCR 2 cut(s) 1014, 1560
EagI CGGCCG 1 cut(s) 1014
Eam1104I CTCTTC 3 cut(s) 365, 895, 1100
Eam1105I GACNNNNNGTC 3 cut(s) 127, 430, 1183
EarI CTCTTC 3 cut(s) 365, 895, 1100
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 1014
Eco130I CCWWGG 6 cut(s) 286, 477, 1113, 1365, 1780, 1995
Eco147I AGGCCT 2 cut(s) 2156, 2210
Eco24I GRGCYC 5 cut(s) 8, 1008, 1688, 1752, 1830
Eco31I GGTCTC 2 cut(s) 114, 2260
Eco47I GGWCC 5 cut(s) 55, 1362, 1522, 1961, 1973
Eco52I CGGCCG 1 cut(s) 1014
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 1157, 2143
Eco88I CYCGRG 2 cut(s) 1850, 2021
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 1968
EcoO109I RGGNCCY 2 cut(s) 1362, 1961
EcoO65I GGTNACC 1 cut(s) 1369
EcoRI GAATTC 5 cut(s) 148, 717, 913, 1156, 1590
EcoRII CCWGG 6 cut(s) 143, 721, 941, 1581, 1969, 2295
EcoT14I CCWWGG 6 cut(s) 286, 477, 1113, 1365, 1780, 1995
EcoT38I GRGCYC 5 cut(s) 8, 1008, 1688, 1752, 1830
EgeI GGCGCC 2 cut(s) 198, 1438
EheI GGCGCC 2 cut(s) 198, 1438
ErhI CCWWGG 6 cut(s) 286, 477, 1113, 1365, 1780, 1995
Esp3I CGTCTC 3 cut(s) 155, 673, 912
FaeI CATG 7 cut(s) 33, 177, 295, 858, 1784, 1825, 1999
FaqI GGGAC 3 cut(s) 1375, 1628, 2240
FatI CATG 7 cut(s) 29, 173, 291, 854, 1780, 1821, 1995
FauI CCCGC 4 cut(s) 475, 563, 1352, 1926
FbaI TGATCA 1 cut(s) 612
FokI GGATG 6 cut(s) 260, 1949, 2072, 2132, 2153, 2176
FriOI GRGCYC 5 cut(s) 8, 1008, 1688, 1752, 1830
FspBI CTAG 5 cut(s) 1394, 1811, 1958, 1965, 1989
GlaI GCGC 4 cut(s) 193, 198, 1384, 1438
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 2 cut(s) 434, 1421
HaeII RGCGCY 2 cut(s) 200, 1440
HaeIII GGCC 7 cut(s) 561, 837, 1016, 1118, 1562, 2156, 2210
HgaI GACGC 4 cut(s) 172, 172, 434, 2235
HhaI GCGC 4 cut(s) 194, 199, 1385, 1439
Hin1I GRCGYC 4 cut(s) 164, 183, 197, 1437
Hin1II CATG 7 cut(s) 33, 177, 295, 858, 1784, 1825, 1999
Hin6I GCGC 4 cut(s) 192, 197, 1383, 1437
HinP1I GCGC 4 cut(s) 192, 197, 1383, 1437
HincII GTYRAC 2 cut(s) 751, 1174
HindII GTYRAC 2 cut(s) 751, 1174
HindIII AAGCTT 1 cut(s) 374
HphI GGTGA 9 cut(s) 433, 751, 885, 1204, 1363, 1565, 1850, 2089, 2198
Hpy166II GTNNAC 7 cut(s) 751, 1174, 1212, 1522, 1573, 1819, 2065
Hpy8I GTNNAC 7 cut(s) 751, 1174, 1212, 1522, 1573, 1819, 2065
Hpy99I CGWCG 5 cut(s) 161, 188, 217, 1379, 2251
HpyAV CCTTC 8 cut(s) 44, 572, 876, 885, 894, 1672, 2167, 2328
HpyCH4III ACNGT 4 cut(s) 354, 650, 1375, 1707
HpyCH4IV ACGT 2 cut(s) 666, 1776
HpyCH4V TGCA 4 cut(s) 140, 935, 974, 2151
HpyF10VI GCNNNNNNNGC 9 cut(s) 12, 189, 567, 932, 988, 1339, 1998, 2092, 2174
HpyF3I CTNAG 5 cut(s) 126, 258, 653, 780, 1007
HpySE526I ACGT 2 cut(s) 666, 1776
Hsp92I GRCGYC 4 cut(s) 164, 183, 197, 1437
Hsp92II CATG 7 cut(s) 33, 177, 295, 858, 1784, 1825, 1999
HspAI GCGC 4 cut(s) 192, 197, 1383, 1437
KasI GGCGCC 2 cut(s) 196, 1436
Kpn2I TCCGGA 1 cut(s) 686
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 612
LguI GCTCTTC 1 cut(s) 365
Lsp1109I GCAGC 7 cut(s) 448, 919, 1000, 1345, 1665, 2079, 2135
MabI ACCWGGT 1 cut(s) 1581
MaeI CTAG 5 cut(s) 1394, 1811, 1958, 1965, 1989
MaeII ACGT 2 cut(s) 666, 1776
MaeIII GTNAC 6 cut(s) 656, 725, 1316, 1369, 1670, 1877
MfeI CAATTG 2 cut(s) 927, 2239
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 8 cut(s) 8, 1008, 1473, 1688, 1752, 1830, 2003, 2341
MlsI TGGCCA 1 cut(s) 1562
MluNI TGGCCA 1 cut(s) 1562
Mly113I GGCGCC 2 cut(s) 197, 1437
MlyI GAGTC 6 cut(s) 240, 463, 1271, 1324, 1842, 2033
MmeI TCCRAC 6 cut(s) 195, 348, 492, 1006, 1284, 1299
Mox20I TGGCCA 1 cut(s) 1562
MroI TCCGGA 1 cut(s) 686
MroNI GCCGGC 1 cut(s) 1037
MroXI GAANNNNTTC 2 cut(s) 582, 1208
MscI TGGCCA 1 cut(s) 1562
MseI TTAA 3 cut(s) 404, 662, 2344
MslI CAYNNNNRTG 1 cut(s) 1779
Msp20I TGGCCA 1 cut(s) 1562
MspA1I CMGCKG 1 cut(s) 570
MteI GCGCNGCGC 1 cut(s) 195
MunI CAATTG 2 cut(s) 927, 2239
Mva1269I GAATGC 1 cut(s) 982
MvaI CCWGG 6 cut(s) 145, 723, 943, 1583, 1971, 2297
MvnI CGCG 4 cut(s) 194, 428, 1754, 1933
MwoI GCNNNNNNNGC 9 cut(s) 12, 189, 567, 932, 988, 1339, 1998, 2092, 2174
NaeI GCCGGC 1 cut(s) 1039
NarI GGCGCC 2 cut(s) 197, 1437
NciI CCSGG 4 cut(s) 773, 1003, 1831, 2101
NcoI CCATGG 2 cut(s) 1780, 1995
NgoMIV GCCGGC 1 cut(s) 1037
NlaIII CATG 7 cut(s) 33, 177, 295, 858, 1784, 1825, 1999
NlaIV GGNNCC 9 cut(s) 198, 271, 622, 947, 1363, 1438, 1787, 1827, 2036
NmeAIII GCCGAG 2 cut(s) 1144, 1400
NmuCI GTSAC 2 cut(s) 1316, 1369
NspV TTCGAA 3 cut(s) 576, 605, 1588
PaeR7I CTCGAG 1 cut(s) 1850
PceI AGGCCT 2 cut(s) 2156, 2210
PciSI GCTCTTC 1 cut(s) 365
PcsI WCGNNNNNNNCGW 3 cut(s) 735, 1190, 1199
PctI GAATGC 1 cut(s) 982
PdiI GCCGGC 1 cut(s) 1039
PdmI GAANNNNTTC 2 cut(s) 582, 1208
PfeI GAWTC 9 cut(s) 244, 578, 607, 776, 862, 1059, 1199, 1303, 1599
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 1965
PfoI TCCNGGA 1 cut(s) 771
PinAI ACCGGT 2 cut(s) 1442, 1524
PleI GAGTC 6 cut(s) 239, 462, 1271, 1323, 1842, 2032
PluTI GGCGCC 2 cut(s) 200, 1440
PpsI GAGTC 6 cut(s) 239, 462, 1271, 1323, 1842, 2032
PpuMI RGGWCCY 2 cut(s) 1362, 1961
PshAI GACNNNNGTC 2 cut(s) 1367, 1861
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 2 cut(s) 1362, 1961
Psp6I CCWGG 6 cut(s) 143, 721, 941, 1581, 1969, 2295
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 6 cut(s) 143, 721, 941, 1581, 1969, 2295
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 9 cut(s) 198, 271, 622, 947, 1363, 1438, 1787, 1827, 2036
PspPI GGNCC 6 cut(s) 55, 836, 1362, 1522, 1961, 1973
PspPPI RGGWCCY 2 cut(s) 1362, 1961
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 595
PsyI GACNNNGTC 1 cut(s) 1965
RsaI GTAC 8 cut(s) 155, 398, 1082, 1427, 1460, 1757, 2066, 2237
RsaNI GTAC 8 cut(s) 154, 397, 1081, 1426, 1459, 1756, 2065, 2236
RseI CAYNNNNRTG 1 cut(s) 1779
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 3 cut(s) 404, 662, 2344
Sau96I GGNCC 6 cut(s) 55, 836, 1362, 1522, 1961, 1973
SchI GAGTC 6 cut(s) 240, 463, 1271, 1324, 1842, 2033
SduI GDGCHC 8 cut(s) 8, 1008, 1473, 1688, 1752, 1830, 2003, 2341
SexAI ACCWGGT 1 cut(s) 1581
SfoI GGCGCC 2 cut(s) 198, 1438
Sfr274I CTCGAG 1 cut(s) 1850
SfuI TTCGAA 3 cut(s) 576, 605, 1588
SgrAI CRCCGGYG 1 cut(s) 761
SinI GGWCC 5 cut(s) 55, 1362, 1522, 1961, 1973
SlaI CTCGAG 1 cut(s) 1850
SmiMI CAYNNNNRTG 1 cut(s) 1779
SmlI CTYRAG 5 cut(s) 1228, 1721, 1790, 1850, 2087
SmoI CTYRAG 5 cut(s) 1228, 1721, 1790, 1850, 2087
SseBI AGGCCT 2 cut(s) 2156, 2210
SspDI GGCGCC 2 cut(s) 196, 1436
SspMI CTAG 5 cut(s) 1394, 1811, 1958, 1965, 1989
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 2 cut(s) 2156, 2210
StyI CCWWGG 6 cut(s) 286, 477, 1113, 1365, 1780, 1995
TaaI ACNGT 4 cut(s) 354, 650, 1375, 1707
TaiI ACGT 2 cut(s) 669, 1779
TaqII GACCGA 1 cut(s) 43
TatI WGTACW 3 cut(s) 1080, 1458, 2064
TauI GCSGC 5 cut(s) 197, 202, 561, 645, 1019
TfiI GAWTC 9 cut(s) 244, 578, 607, 776, 862, 1059, 1199, 1303, 1599
Tru1I TTAA 3 cut(s) 404, 662, 2344
Tru9I TTAA 3 cut(s) 404, 662, 2344
TscAI CASTG 3 cut(s) 516, 1413, 1473
TseFI GTSAC 2 cut(s) 1316, 1369
TseI GCWGC 7 cut(s) 436, 932, 988, 1333, 1653, 2092, 2148
Tsp45I GTSAC 2 cut(s) 1316, 1369
TspDTI ATGAA 3 cut(s) 162, 2291, 2310
TspGWI ACGGA 5 cut(s) 121, 571, 693, 1310, 1494
TspRI CASTG 3 cut(s) 516, 1413, 1473
Tth111I GACNNNGTC 1 cut(s) 1965
VpaK11BI GGWCC 5 cut(s) 55, 1362, 1522, 1961, 1973
XagI CCTNNNNNAGG 1 cut(s) 1968
XapI RAATTY 8 cut(s) 148, 681, 717, 913, 1156, 1590, 1903, 2303
XhoI CTCGAG 1 cut(s) 1850
XmnI GAANNNNTTC 2 cut(s) 582, 1208
XspI CTAG 5 cut(s) 1394, 1811, 1958, 1965, 1989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.